African Journal of Microbiology Research
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Article Number - 73972C065507


Vol.11(30), pp. 1200-1210 , August 2017
DOI: 10.5897/AJMR2017.8588
ISSN: 1996-0808



Full Length Research Paper

Production of novel antifungal compounds from actinomycetes isolated from waste dump soil in Western Uganda



Adamu Almustapha Aliero
  • Adamu Almustapha Aliero
  • Department of Microbiology and Immunology, Kampala International University, Western Campus, Uganda.
  • Google Scholar
Ibrahim Ntulume
  • Ibrahim Ntulume
  • Department of Microbiology and Immunology, Kampala International University, Western Campus, Uganda.
  • Google Scholar
John Odda
  • John Odda
  • Department of Pharmacology, Kampala International University, Western Campus, Uganda.
  • Google Scholar
Matilda Angela Okech
  • Matilda Angela Okech
  • College of Health, Medicine and Life Sciences, Department of Microbiology, St. Augustine International University, Kampala, Uganda.
  • Google Scholar







 Received: 13 May 2017  Accepted: 18 July 2017  Published: 14 August 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Drug resistant and opportunistic organisms are a problem to medical health due to the fact that most of the drugs that were used are now not effective. Currently, there is a need to search for new drugs that can enhance the control of these organisms. Actinomycetes and their secondary metabolites can be used as such drugs. This study was designed to isolate actinomycetes producing novel anti-fungal compounds from waste dump soil in Western Uganda. Fifty six (56) actinomycetes were isolated from 22 waste dump soil samples. All isolates were screened using modified spektra - plak method against Candida albicans ATCC1023, Penicillium sp., Aspergillus sp., Fusarium sp. and Rhizopus sp. Eight 8(14.29%) isolates showed antifungal activity to at least one test fungi during primary screening. Two isolates [KBRWDSa (FR) and KBMWDSb6] showed activity to all test fungi. Secondary screening was carried out by growing all 56 isolates in broth and their supernatant was tested for antifungal activity using agar well diffusion method. 11(19.64%) of these isolates showed activity against at least one test fungi with mean zone of inhibition 5.33 to 29.69 mm. Isolate KBMWDSb6 showed a broad spectrum activity against all test fungi. The remaining broths were extracted using ethanol. The ethanol extract at 2.5 mg/ml concentration was also tested for antifungal activity using agar well diffusion method. 13 (23.21%) isolates showed activity against at least one test fungi with mean zone of inhibition 6.33 to 30.67mm. The findings showed that some of these isolates had antifungal activity.

 

Key words: Waste dump soil, actinomycetes, novel anti-fungal compounds, Western Uganda.

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APA Aliero, A. A., Ntulume, I., Odda, J., & Okech, M. A. (2017). Production of novel antifungal compounds from actinomycetes isolated from waste dump soil in Western Uganda. African Journal of Microbiology Research, 11(30), 1200-1210.
Chicago Adamu Almustapha Aliero, Ibrahim Ntulume, John Odda and Matilda Angela Okech. "Production of novel antifungal compounds from actinomycetes isolated from waste dump soil in Western Uganda." African Journal of Microbiology Research 11, no. 30 (2017): 1200-1210.
MLA Adamu Almustapha Aliero, et al. "Production of novel antifungal compounds from actinomycetes isolated from waste dump soil in Western Uganda." African Journal of Microbiology Research 11.30 (2017): 1200-1210.
   
DOI 10.5897/AJMR2017.8588
URL http://www.academicjournals.org/journal/AJMR/article-abstract/73972C065507

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