African Journal of Microbiology Research
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Article Number - 73972C065507

Vol.11(30), pp. 1200-1210 , August 2017
ISSN: 1996-0808

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Full Length Research Paper

Production of novel antifungal compounds from actinomycetes isolated from waste dump soil in Western Uganda

Adamu Almustapha Aliero
  • Adamu Almustapha Aliero
  • Department of Microbiology and Immunology, Kampala International University, Western Campus, Uganda.
  • Google Scholar
Ibrahim Ntulume
  • Ibrahim Ntulume
  • Department of Microbiology and Immunology, Kampala International University, Western Campus, Uganda.
  • Google Scholar
John Odda
  • John Odda
  • Department of Pharmacology, Kampala International University, Western Campus, Uganda.
  • Google Scholar
Matilda Angela Okech
  • Matilda Angela Okech
  • College of Health, Medicine and Life Sciences, Department of Microbiology, St. Augustine International University, Kampala, Uganda.
  • Google Scholar

 Received: 13 May 2017  Accepted: 18 July 2017  Published: 14 August 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0

Drug resistant and opportunistic organisms are a problem to medical health due to the fact that most of the drugs that were used are now not effective. Currently, there is a need to search for new drugs that can enhance the control of these organisms. Actinomycetes and their secondary metabolites can be used as such drugs. This study was designed to isolate actinomycetes producing novel anti-fungal compounds from waste dump soil in Western Uganda. Fifty six (56) actinomycetes were isolated from 22 waste dump soil samples. All isolates were screened using modified spektra - plak method against Candida albicans ATCC1023, Penicillium sp., Aspergillus sp., Fusarium sp. and Rhizopus sp. Eight 8(14.29%) isolates showed antifungal activity to at least one test fungi during primary screening. Two isolates [KBRWDSa (FR) and KBMWDSb6] showed activity to all test fungi. Secondary screening was carried out by growing all 56 isolates in broth and their supernatant was tested for antifungal activity using agar well diffusion method. 11(19.64%) of these isolates showed activity against at least one test fungi with mean zone of inhibition 5.33 to 29.69 mm. Isolate KBMWDSb6 showed a broad spectrum activity against all test fungi. The remaining broths were extracted using ethanol. The ethanol extract at 2.5 mg/ml concentration was also tested for antifungal activity using agar well diffusion method. 13 (23.21%) isolates showed activity against at least one test fungi with mean zone of inhibition 6.33 to 30.67mm. The findings showed that some of these isolates had antifungal activity.


Key words: Waste dump soil, actinomycetes, novel anti-fungal compounds, Western Uganda.

Alireza D, Laleh PY, Rouhollah B, Ahad M, Samad A, Ali RM, Gasanova S, Rahib A (2010). Investigation antibacterial activity of Streptomycetes isolates from soil samples, West of Iran. Afr. J. Microbiol. Res. 4(16):1685-1693.


Arendrup MC (2014). Update on antifungal resistance in Aspergillus and Candida. Clin. Microbiol. Infect. 20(s6):42-48.


Arifuzzaman M, Khatun MR, Rahman H (2010). Isolation and screening of actinomycetes from Sundarbans soil for antibacterial activity. Afr. J. Biotechnol. 9:4615-4619.


Baddley JW, Patel M, Bhavnani SM, Moser SA, Andes DR (2008). Association of fluconazole pharmacodynamics with mortality in patients with candidemia. Antimicrob. Agents Chemother. 52:3022-3028.


Basilio A, Vicente MF, Gorrochategui J, Gonzalez I, Cabella A, Gonzalez A, Genilloud O (2003). Patterns of antimicrobial activities from soil actinomycetes isolated under different conditions of pH and salinity. J. Appl. Microbiol. 95(4):814-823.


Buchanan RE, Gibbons NE, Cowan ST, Holt JG, Liston J, Murary RGE, Niven CF, Ravin AW, Stainer eds. Bergey's RY (1974). Manual of Determinative Bacteriology, eighth edn, Williams &Wilkins Company, Baltimore, U.S.A. pp. 659-881.


CDC-Centre for Disease Control and Prevention (2016). Fungal diseases: Antifungal resistant. Available at: 

View Accessed 16/11/2016


Cheesebrough M (2006). District laboratory practice in tropical countries. Part 2. Low Price edition. Cambridge University Press, London.


Chowdhary A, Agarwal K, Kathuria S, Singh PK, Roy P, Gaur SN, de Hoog GS, Meis JF (2013). Clinical significance of filamentous Basidiomycetes, illustrated by the novel opportunist Ceriporialacerata isolated from the human respiratory tract. J. Clin Microbiol. 51:585-590.


Ekeke C, Okonwu K (2013). Comparative Study on Fertility Status of Soils of University of Port Harcourt, Nigeria. Res. J. Bot. 8:24-30.


Ensieh S, Maryam N (2016). Isolation of biologically active Actinomycetes from untouched soils: a case study from Karaj district, Iran. Prog. Biol. Sci. 6(1):65-74.


Ensieh S, Mona S, Vida T (2015). Antibacterial activity of some actinomycetes isolated from soils of Alborz province, Iran. Prog. Biol. Sci. 5(2):159-167.


George M, Anjumol A, George G, Mohamed HAA (2012). Distribution and bioactive potential of soil actinomycetes from different ecological habitats. Afr. J. Microbiol. Res. 6(10):2265-2271.


Hotam SC, Jayprakash YAR, Shrivastava SS, Anil KS, Natrajan G (2013). Antibacterial activity of actinomycetes isolated from different soil samples of Sheopur (A city of central India). J. Adv. Pharm. Technol. Res. 4(2):118-123.


Howard SJ, Cerar D, Anderson MJ, Albarrag A, Fisher MC, Pasqualotto AC, Laverdiere M, Arendrup MC, Perlin DS, Denning DW (2009). Frequency and evolution of Azole resistance in Aspergillus fumigatus associated with treatment failure. Emerg. Infect. Dis. 15:1068-1076.


Ismail S, Ban A, Racha A (2015). Testing of Production of Inhibitory Bioactive Compounds by Soil Streptomycetes as Preliminary Screening Programs in UAE for Anti-Cancer and Anti-Bacterial Drugs. Int. J. Curr. Microbiol. Appl. Sci. 4:446-459.


Jagan MY, Sirisha B, Prathyusha K, Pola SR (2014). Isolation, Screening and Characterization of Actinomycetes from Marine Sediments for their Potential to Produce Antifungal Agents. Glob. J. Biol. Agric. Health Sci. 3(4):131-137.


Jemimah NS, Nasimunislam N, Vaishnavi B, Mohanasrinivasan V, Subathra DC (2012). Isolation of Soil actinomycetes inhabiting Amrithi forest for the potential source of bioactive compounds. Asian J. Pharm. Clin. Res. 5:189-192.


Jirayut E, Arjaree N, Srisurang T, Takuya N, Yusuhiro I, Watanalai P (2012). Identification and characterization of soil-isolated Streptomyces SJE177 producing actinomycin. Southeast Asian J. Trop. Med. Public Health 41(5):1177.


Kekuda PTR, Shobha KS, Onkarappa R, Gautham SA, Raghavendra HL (2012). Screening biological activities of a Streptomyces species isolated from soil of Agumbe, Karnataka, India. Int. J. Drug Dev. Res. 4(3):104-114.


Lakshmanaperumalsamy P, Chandramohan D, Natarajan R (1984). Seasonal variation of microbial population from sediments of Vellar estuary, South India. In. 2. Colloque International de Bacteriologie Marine, Brest (France), pp. 1-5.


Lakshmipathy D, Krishnan K (2010). Antagonistic activity of Streptomyces VITDDK1 spp. (GU223091) isolated from the coastal region of Tamil Nadu, India. Pharmacologyoline 1:17-29.


Lortholary O, Desnos-Ollivier M, Sitbon K, Fontanet A, Bretagne S, Dromer F, French Mycosis Study Group (2011). Recent exposure to caspofungin or fluconazole influences the epidemiology of candidemia: a prospective multicenter study involving 2441 patients. Antimicrob. Agents Chem. 55:532-538.


Madigan MT, Martiko JM, Parker J (1997). Antibiotics: Isolation and characterization. In. Brook Biology of Microorganisms, 8th edn. Prentice-Hall International Inc. New Jersey, pp. 440-442.


Masna R, Nisha B, Nisha D, Pappu KM (2016). Isolation of antibiotic producing Actinomycetes from soil of Kathmandu valley and assessment of their antimicrobial activities. Int. J. Microbiol. Allied Sci. 2(4):22-26.


Mincer TJ Jensen PR, Kauffman CA, Fenical W (2002). Widespread and persistent of population of a major actinomycetes taxon in ocean sediments. Appl. Environ. Microbiol. 68(10):5005-5011.


Monisha K, Renu S, Rup L (2011). Selective isolation of rare actinomycetes producing novel antimicrobial compounds. Int J. Adv Biotechnol. Res. 2:357-375.


Morgan J, Meltzer MI, Plikaytis BD, Sofair AN, Huie-White S, Wilcox S, Harrison LH, Seaberg EC, Hajjeh RA, Teutsch SM (2005). Excess mortality, hospital stay, and cost due to candidemia: a case-control study using data from population-based candidemia surveillance. Infect. Control Hosp. Epidemiol. 26:540-547.


Moselio S, Joshua L (2004). The desk Encyclopedia of Microbiology, Elsevier Academic. Press, California, Amsterdam.


Nalubega Fatuma, Meklat Atika, Mellouk Imène, Chaabane Chaouch Fawzia, Verheecke Caro, Bouras Noureddine, Mokrane Salim, Mathieu Florence and Sabaou Nasserdine (2016). Taxonomy and antagonistic activity of A12, a new Streptomyces strain isolated from Ugandan soil. ElWahat pour les Recherches et les Etudes 9 (1):1 - 11


Oh BJ, Shin JH, Kim MN, Sung H, Lee K, Joo MY, Shin MG, Suh SP, Ryang DW (2011). Biofilm formation and genotyping of Candida haemulonii, Candida pseudohaemulonii, and a proposed new species (Candida auris) isolates from Korea. Med. Mycol. 49:98-102.


Oskay M, Tamer A, Azeri C (2004). Antibacterial activity of some actinomycetes isolated from farming soils of Turkey. Afr. J. Biotechnol. 3:441-446.


Parkes-Ratanshi RB, Achan B, Kambugu A, Meya D, Denning D (2013). Estimated burden of fungal disease in Ugand. 23rd European Congress of Clinical Microbiology and Infectious Diseases. Berlin, Abstract 3635.


Raja A, Prabakaran P (2011). Preliminary screening of antimycobacterial effect of psychrophilic actinomycetes isolated from Manali ice point Himachal Predesh. J. Microbiol. Antimicrob. 3(2):41-46.


Remya M, Vijayakumar R (2008). Isolation and Characterization of marine antagonistic actinomycetes from west coast of India. Med. Biol. 15:13-19.


Rinoy V, Nishamol S, Suchithra R, Jyothy S, Mohamed HD AA (2012). Distribution and Antibacterial Activity of Actinomycetes from Shola Soils of Tropical Montane Forest in Kerala, South India. J. Environ. 1(3):93-99.


Rotich MC, Magiri E, Bii C, Maina N (2017). Bio-Prospecting for Broad Spectrum Antibiotic Producing Actinomycetes Isolated from Virgin Soils in Kericho County, Kenya. Adv. Microbiol. 7:56-70.


Satoh K, Makimura K, Hasumi Y, Nishiyama Y, Uchida K, Yamaguchi H (2009). Candida auris sp. nov., a novel ascomycetous yeast isolated from the external ear canal of an inpatient in a Japanese hospital. Microbiol. Immunol. 53:41-44.


Smith KD, Achan B, Hullsiek KH, McDonald TR, Okagaki LH, Alhadab AA, Akampurira A, Rhein JR, Meya DB, Boulware DR, Nielsen K, ASTRO-CM/COAT Team (2015). Increased antifungal drug resistance in clinical isolates of Cryptococcus neoformans in Uganda. Antimicrob Agents Chemother. Am. Soc. Microbiol. 59:7197-7204.


Sengupta S, Pramanik A, Ghosh A, Bhattacharyya M (2015). Antimicrobial activities of actinomyceties isolated from unexflored regions of Sundarbans mangrove ecosystem. BMC Microbiol. 15(1):170.


Sundaramoorthi C, Vengadesh PK, Gupta S, Karthic K, Tamilsehli V (2011). Production and characterization of antibiotics from soil–isolated actinomycetes. Int. Res. J. Pharm. 2:114-118.


Tara DG, Chringma S, Vishwanath PA, Binod L (2009). Isolation and Characterization of Antibacterial Actinomycetes from Soil Samples of Kalapatthar, Mount Everest Region. Nepal J. Sci. Technol. 10:173-182.


Tiwarty KB (2009). Protocol for actinomycetes studies in RLABB. 2008/9. Central department of microbiology, institution of science and technology. Tribhuven University, kiripur. P 21. (2011). Triple sugar Iron agar. Available 

View.Retrieved 12 November, 2016.



APA Aliero, A. A., Ntulume, I., Odda, J., & Okech, M. A. (2017). Production of novel antifungal compounds from actinomycetes isolated from waste dump soil in Western Uganda. African Journal of Microbiology Research, 11(30), 1200-1210.
Chicago Adamu Almustapha Aliero, Ibrahim Ntulume, John Odda and Matilda Angela Okech. "Production of novel antifungal compounds from actinomycetes isolated from waste dump soil in Western Uganda." African Journal of Microbiology Research 11, no. 30 (2017): 1200-1210.
MLA Adamu Almustapha Aliero, et al. "Production of novel antifungal compounds from actinomycetes isolated from waste dump soil in Western Uganda." African Journal of Microbiology Research 11.30 (2017): 1200-1210.

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