African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4837

Full Length Research Paper

Clinical microbiology study of diabetic foot ulcer in Iran; pathogens and antibacterial susceptibility

Nahid Rouhipour1, Alireza Hayatshahi2, Mohsen Khoshniat Nikoo1, Nika Mojahed Yazdi1, Ramin Heshmat1, Mostafa Qorbani3,4, Masoud Mehrannia5, Abolfazl Shojaifard6, Farzaneh Abbasi1, Seyed Mohammad Tavangar7, Mohammad Reza Mohajeri Tehrani1and Bagher Larijani1*
  1Endocrinology, Endocrinology and Metabolism Research Center (EMRC) Tehran University of Medical Sciences, Tehran, Iran. 2Pharmacotherapy Department, Shariati Hospital, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. 3Faculty of Golestan University of Medical Sciences, Iran. 4Epidemiology, Tehran University of Medical Sciences, Tehran, Iran. 5Division of Public Health, Iran University of Medical Sciences, Tehran, Iran. 6Division of Surgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. 7Division of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Email: [email protected]

  •  Accepted: 16 March 2012
  •  Published: 19 July 2012

Abstract

 

The aim of this study was to investigate microbial pathogens and their antibiotic susceptibility profile in infected diabetic foot ulcers in Iranian patients. This was a one-year cross sectional study on diabetic patients with infected diabetic foot ulcer at Shariati Teaching Hospital, Tehran, Iran. Grade of ulcer was determined by Wagner’s criteria. Specimens were obtained from the base of ulcer, deep part of the wound or aspiration and were tested with gram staining and antibacterial susceptibility was determined with both disk diffusion and E-Test methods. Total of 546 pathogens were isolated from 165 ulcers of 149 patients. Gram positive aerobes including Enterococcal species and methicillin resistant Staphylococcus aureus (S. aureus) (21.4 and 19.4%, respectively) were identified as the most common pathogens followed by Gram negative isolates including Escherichia coli and Pseudomonas-aeruginosa (12.6 and 5.4%, respectively). The majority of wounds were classified as Wagner grades 2 and 3 (15.7 and 75.7%). Appropriate empiric treatment to cover both these Gram positive and Gram negative pathogens is crucially important.

 

Key words: Diabetic foot ulcer, Enterococcus, Staphylococcus.

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