African Journal of Microbiology Research
Subscribe to AJMR
Full Name*
Email Address*

Article Number - 6F11A2E65511


Vol.11(30), pp. 1211-1217 , August 2017
DOI: 10.5897/AJMR2017.8592
ISSN: 1996-0808



Full Length Research Paper

Microbiological monitoring of water and Crassostrea rhizophorae in a mangrove ecosystem in Brazil



Dandara S. Cabral
  • Dandara S. Cabral
  • University of Vila Velha, Avenida Comissário José Dantas de Melo, nº 21, Boa Vista Vila Velha - ES - CEP: 29102-920, Brazil.
  • Google Scholar
Juliano O. Barbirato
  • Juliano O. Barbirato
  • University of Vila Velha, Avenida Comissário José Dantas de Melo, nº 21, Boa Vista Vila Velha - ES - CEP: 29102-920, Brazil.
  • Google Scholar
Clarisse M. Arpini
  • Clarisse M. Arpini
  • University of Vila Velha, Avenida Comissário José Dantas de Melo, nº 21, Boa Vista Vila Velha - ES - CEP: 29102-920, Brazil.
  • Google Scholar
Barbara D. Barcellos
  • Barbara D. Barcellos
  • Federal University of Viçosa. Avenida: Peter Henry Rolfs, s/n - Campus Universitário, Viçosa – Minas Gerais, 36570-900, Brazil.
  • Google Scholar
Katherine F. Ruas
  • Katherine F. Ruas
  • State University do Norte Fluminense Darcy Ribeiro. Avenida Alberto Lamego, 2000 - Parque California, Campos dos Goitacazes – Rio de Janeiro, 28035-200, Brazil.
  • Google Scholar
Leonardo B. Dobbss
  • Leonardo B. Dobbss
  • Federal University of Vales do Jequitinhonha e Mucuri, Institute of Agricultural Sciences, Avenida Vereador Joao Narciso, 1380, 38610-000, Unai, Minas Gerais, Brazil.
  • Google Scholar







 Received: 16 May 2017  Accepted: 31 July 2017  Published: 14 August 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Mangroves are considered as natural nurseries and  classified as permanent protected areas. The study area is the municipal ecological site of Ilha do Lameirão, covering 891.83 ha of mangrove forest. This area  persistently suffers from anthropic pressure. For this reason, there is the need to study and monitor the microbiological characteristics and recognition of  the main factors involved in the degradation of mangroves process. This study was done in the year 2014. Samples were taken every month during  dry and rainy seasons. Two areas (Maria Ortiz and Canal dos Escravos) were chosen based on the degree of their vulnerability  to anthropic pressure. The results show there is a microbiological contamination both in water and Crassostrea rhizophorae tissues, mainly in samples taken from Maria Ortiz. According to the results, the Canal dos Escravos estuary, known as Class 1 is an environment that is able to maintain aquatic communities and fishing activities. In oyster tissues, microorganism strains that indicate the pathogens which cause diseases in humans were identified.

 

Key words: Environmental quality, microbiology, anthropic, pollution, oyster.

American Public Health Association (APHA) (1970). Recommended Procedures for the Examination of Seawater and Shellfish. fourth ed. American Public Health Association, Washington DC, US. 1–47.

 

Brazil (2001). ANVISA. Regulamento técnico sobre os padrõesmicrobiológicos para alimentos. Resolução RDC n.12, de 02 de janeiro de 2001. Ministério da Saúde: Agência Nacional de Vigilância Sanitária, Diário Oficial da União Available at: 

View (Accessed December 2014).

 
 

Brazil (2005). CONAMA. Dispõe sobre a classificação dos corpos de água e diretrizes ambientais para o seu enquadramento, bem como estabelece as condições e padrões de lançamento de efuentes, e dá outras providências. Resolução Conama n. 357, de 17 de março de 2005. Ministério do Meio Ambiente: Conselho Nacional do Meio Ambiente, Diário Oficial da União Available at: 

View (Accessed December 2014).

 
 

Caffrey JM, Hollibaugh JT, Mortazavi B (2016). Living oysters and their shells as sites of nitrification and denitrification. Mar. Pollut. Bull. 112:86-90
Crossref

 
 

Corporeau C, Vanderplancke G, Boulais M, Suquet M, Quéré C, Boudry P, Huvet A, Madec, S (2012). Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas. J. Proteomics 75:5554-5563.
Crossref

 
 

Edun OM, Efiuvwevwere BJO (2012). Bacterial Profiles and Physico-chemical Parameters of Water Samples from Different Sites of the New Calabar River, Nigeria. Sustain. Agric. Res. 1(2):162.
Crossref

 
 

Evangelista-Barreto NS, Pereira, AF Silva, RAR, Ferreira LTB (2014). Presence of antimicrobial resistant pathogens in oysters and mussels from Iguape Bay, Maragogipe (Bahia). Rev. Acad. Agrár. Ambient. 12(1):25-34.

 
 

ISO (2015). ISO 16649-3 (2015). Microbiology of the Food Chain – Horizontal Method for the Enumeration of Beta-Glucuronidase-Positive Escherichia coli – Part 3: Detection and most Probable Number Technique Using 5-Bromo-4-Chloro-3-Indolyl-Beta-DGlucuronide. International Organization for Standardization, Geneva.

 
 

Iwamoto M, Ayers T, Mahon BE, Swerdlow DL (2010). Epidemiology of sea food associated infections in the United States. Clin. Microbiol. Rev. 23:399-411.
Crossref

 
 

Kolm HE, Miquelante FA (2011). Indicadores microbiológicos de poluição fecal na desembocadura da Gamboa Olho d'água, Paraná: subsídio para o monitoramento da balneabilidade do Brazil. Publicatio UEPG Biolological and Health Sciences. 17(1):21-35.
Crossref

 
 

Komeily A, Srinivasan RS (2015). A need for balanced approach to neighbourhood sustainability assessments: A critical review and analysis. Sustain. Cities Soc. 18:32-43.
Crossref

 
 

Lee RJ, Younger AD (2003). Determination of the relationship between faecal indicator concentrations and the presence of human pathogenic micro-organisms in shellfish. Molluscan Shellfish Safety, Galicia: Grafinova S.A. 247-252.

 
 

Machado IC, Paula AMR, Buzzo A, Jakabi M, Ristori C, Sakuma H (2001). Study of the occurrence of organic contamination in the Cananéia-Säo Paulo estuary, as subsidy for the extraction, handling and cultivation of the oyster of the swamp (Crassostrea brasiliana): analysis of oyster (soft tissues and intervalvar liquid. Hig. Alim. 15(83):44-48.

 
 

Mok JS, Yoo HD, Kim PH, Yoon HD, Park YC, Lee TS, Kwon JY, Son KT, Lee HJ, Ha KS, Shim, KB, Kim, JH (2015). Bioaccumulation of heavy metals in oysters from the southern coast of Korea: assessment of potential risk to human health. Bull. Environ. Contam. Toxicol. 94:749-755.
Crossref

 
 

Morelli AMF, Vieira RHSF (2003) Indicadores de contaminação fecal para ostra-do-mangue (Crassostrea rhizophorae) comercializadas na Praia do Futuro, Fortaleza, Ceará. Hig. Alim. 17:81-88.

 
 

Moura AC, Assumpção RAB, Bischoff J (2009). Monitoramento físico-químico e microbiológico da água do rio Cascavel durante o período de 2003 a 2006. Arq. Inst. Biol. 76:17-22.

 
 

Ponce E, Khan AA, Cheng CM, Summage-West C and Cerniglia CE (2008). Prevalence and characterization of Salmonella enteric serovar Weltevreden from imported seafood. Food Microbiol. 25:29-35.
Crossref

 
 

Potasman I, Paz A, Odeh M (2002). Infectious outbreaks associated with bivalve shellfish consumption: A worldwide perspective. Clin. Infect. Dis. 35:921-928.
Crossref

 
 

Rampersad FS, Laloo S, La Borde A, Maharaj K, Sookhai L, Teelucksingh J, Reid S, McDougall L, Adesiyun AA (1999). Microbial quality of oysters sold in Western Trinidad and potential health risk to consumers. Epidemiol. Infect. 123:241-250.
Crossref

 
 

Sanchez-Vargas M, Abu-El-Haija MA, Gómez-Duarte OG (2011). Salmonella infections: An update on epidemiology, management, and prevention. Travel. Med. Infect. Dis. 9(6):1-15.
Crossref

 
 

Schaeffer-Novelli Y (1995). Manguezal: ecossistema entre a terra e o mar. São Paulo: Caribbean Ecological Research. pp.13-15.

 
 

Silva AIM, Vieira RHSF, Menezes FGR, Fonteles-Filho A, Torres RCO, Santanna ES (2004). Bacteria of fecal origin in mangrove oysters (Crassostrea rhizophorae) in the Coco River estuary, Ceará State, Brazil. Braz. J. Microbiol. 35:126-130.
Crossref

 
 

Sroczyńska K, Barroso G, Chícharo L (2012). In situ effective clearance rate measurement of mangrove oysters (Crassostrea rhizophorae) in a tropical estuary in Brazil. Ecohydrol. Hydrobiol. 12(4):301-310.
Crossref

 
 

Suplicy FA (2000). Capacidade de suporte nos cultivos de moluscos: está no Decreto, mas poucos sabem exatamente do que se trata. Pan. aquicul. 10:21-24.

 
 

Tulli LMA (2007). Vulnerabilidade à ação antrópica e uso e ocupação do solo para a Estação Ecológica Municipal Ilha do Lameirão, Vitória-ES. (M.Sc. Dissertation Universidade Federal do Espírito Santo. UFES). 72p.

 
 

Vieira RHSF, Atayde MA, Carvalho EMR, Carvalho FCT, Fonteles Filho, AA (2008). Contaminação fecal da ostra Crassostrea rhizophorae e da água de cultivo do Rio Pacoti (Eusébio, Estado do Ceará): Isolamento e Identificação de Escherichia coli e sua susceptibilidade a diferentes antimicrobianos. Braz. J. Vet. Res. Anim. Sci. 45:180-189.
Crossref

 
 

Whitman KA (2004). Finfish and shellfish bacteriology manual: techniques and procedures. Wiley-Blackwell, Iowa, EUA. 258.

 
 

Wilson JG (2002). Productivity, Fisheries and Aquaculture in temperate estuaries. ‎ Estuar. Coast Shelf Sci. 55:953-967.
Crossref

 
 

Zar JH (1999). Biostatistical analysis. Prentice Hall, New Jersey, EUA. 663.

 

 


APA Cabral, D. S., Barbirato, J. O., Arpini, C. M., Barcellos, B. D., Ruas, K. F., & Dobbss, L. B. (2017). Microbiological monitoring of water and Crassostrea rhizophorae in a mangrove ecosystem in Brazil. African Journal of Microbiology Research, 11(30), 1211-1217.
Chicago Dandara S. Cabral,  Juliano O. Barbirato, Clarisse M. Arpini, B&arbara D. Barcellos, Katherine F. Ruas and Leonardo B. Dobbss. "Microbiological monitoring of water and Crassostrea rhizophorae in a mangrove ecosystem in Brazil." African Journal of Microbiology Research 11, no. 30 (2017): 1211-1217.
MLA D, et al. "Microbiological monitoring of water and Crassostrea rhizophorae in a mangrove ecosystem in Brazil." African Journal of Microbiology Research 11.30 (2017): 1211-1217.
   
DOI 10.5897/AJMR2017.8592
URL http://www.academicjournals.org/journal/AJMR/article-abstract/6F11A2E65511

Subscription Form