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Article Number - 57A72DE55259


Vol.11(47), pp. 1669-1675 , December 2017
https://doi.org/10.5897/AJMR2017.8677
ISSN: 1996-0808


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Full Length Research Paper

Genotypic characterization of Escherichia coli strains isolated from dairy cattle environment



Greiciane Franca Bronzato
  • Greiciane Franca Bronzato
  • Veterinary Bacteriology Laboratory, Federal Rural University of Rio de Janeiro, UFRRJ, Rio de Janeiro, Brazil.
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Naiara Miranda Bento Rodrigues
  • Naiara Miranda Bento Rodrigues
  • Veterinary Bacteriology Laboratory, Federal Rural University of Rio de Janeiro, UFRRJ, Rio de Janeiro, Brazil.
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Bruno Rocha Pribul
  • Bruno Rocha Pribul
  • National Reference Laboratory for Enteric Diseases, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
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Gabrielli Stefaninni Santiago
  • Gabrielli Stefaninni Santiago
  • Veterinary Bacteriology Laboratory, Federal Rural University of Rio de Janeiro, UFRRJ, Rio de Janeiro, Brazil.
  • Google Scholar
Irene da Silva Coelho
  • Irene da Silva Coelho
  • Veterinary Bacteriology Laboratory, Federal Rural University of Rio de Janeiro, UFRRJ, Rio de Janeiro, Brazil.
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Miliane Moreira Soares de Souza
  • Miliane Moreira Soares de Souza
  • Veterinary Bacteriology Laboratory, Federal Rural University of Rio de Janeiro, UFRRJ, Rio de Janeiro, Brazil.
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Elina Reinoso
  • Elina Reinoso
  • Microbial Genetics Laboratory, National University of Rio Cuarto, UNRC, Río Cuarto, Argentina.
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Mirta Lasagno
  • Mirta Lasagno
  • Microbial Genetics Laboratory, National University of Rio Cuarto, UNRC, Río Cuarto, Argentina.
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Shana de Mattos de Oliveira Coelho
  • Shana de Mattos de Oliveira Coelho
  • Veterinary Bacteriology Laboratory, Federal Rural University of Rio de Janeiro, UFRRJ, Rio de Janeiro, Brazil.
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 Received: 13 August 2017  Accepted: 15 November 2017  Published: 21 December 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The aim of this study was to characterize the diversity of Escherichia coli strains involved in the dispersion of virulence genes. 152 E. coli strains originated from dairy cattle environment were evaluated through phenotypic and proteomic assays. These samples were investigated for the presence of virulence genes (eaeA, stxI, stxII, ST, LT, eagg, ial) and biofilm related genes (fimH, csgA, flu). Eighteen profiles were obtained and 30 isolates were selected for macrorestriction assay by Pulsed-Field Gel Electrophoresis (PFGE) technique. PFGE patterns of XbaI-digested performed to determine the clonal relatedness of E coli isolates. A total of 27 pulsotypes of E. coli were identified with a low percentage of genetic similarity (≤68%) demonstrating a high genetic diversity in the isolates tested. Furthermore, the presence of biofilm-associated related gene (fimH) can contribute to the dispersion and persistence of this pathogen into the milk environment. Also, all of the pulsotypes (n = 8) with genetic similarity from 95% were present in milk samples, feces and liner were found in different seasons of the year, sharing at least one gene associated with biofilm formation. So the characterization of the genetic diversity of E. coli is important for understanding its dispersion in order to assist in the implementation of measures to prevent the spread of these agents in milk production environment.

 

Key words: circulating clones, diversity, Escherichia coli, persistence, Pulsed-Field Gel Electrophoresis (PFGE), virulence gene.

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APA Bronzato, G. F., Rodrigues, N. M. B., Pribul, B. R., Santiago, G. S., Coelho, I. S., de Souza, M. M. S., Reinoso, E., Lasagno, M., & Coelho, S. M. O. (2017). Genotypic characterization of Escherichia coli strains isolated from dairy cattle environment. African Journal of Microbiology Research, 11(47), 1669-1675.
Chicago Greiciane França Bronzato, Naiara Miranda Bento Rodrigues, Bruno Rocha Pribul, Gabrielli Stefaninni Santiago, Irene da Silva Coelho, Miliane Moreira Soares de Souza, Elina Reinoso, Mirta Lasagno and Shana de Mattos de Oliveira Coelho. "Genotypic characterization of Escherichia coli strains isolated from dairy cattle environment." African Journal of Microbiology Research 11, no. 47 (2017): 1669-1675.
MLA Greiciane Franccedil;a Bronzato, et al. "Genotypic characterization of Escherichia coli strains isolated from dairy cattle environment." African Journal of Microbiology Research 11.47 (2017): 1669-1675.
   
DOI https://doi.org/10.5897/AJMR2017.8677
URL http://www.academicjournals.org/journal/AJMR/article-abstract/57A72DE55259

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