African Journal of Microbiology Research
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Article Number - 30241CE55263


Vol.11(47), pp. 1681-1687 , December 2017
DOI: 10.5897/AJMR2017.8715
ISSN: 1996-0808



Full Length Research Paper

Phytochemical screening and antifungal activity of leaves extracts of Luffa cylindrica (Roem)



Mercy Itohan Aboh
  • Mercy Itohan Aboh
  • Department of Microbiology, Human Virology and Biotechnology, National Institute for Pharmaceutical Research and Development, Idu, P. M. B. 21 Garki Abuja, Nigeria.
  • Google Scholar
Peters Oladosu
  • Peters Oladosu
  • Department of Microbiology, Human Virology and Biotechnology, National Institute for Pharmaceutical Research and Development, Idu, P. M. B. 21 Garki Abuja, Nigeria.
  • Google Scholar
Gbonbola Adeshina
  • Gbonbola Adeshina
  • Department of Pharmaceutics and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
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Busayo Olayinka
  • Busayo Olayinka
  • Department of Pharmaceutics and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
  • Google Scholar
Stephen Olayeni Olonitola
  • Stephen Olayeni Olonitola
  • Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
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 Received: 20 September 2017  Accepted: 21 October 2017  Published: 21 December 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Mycosis constitutes a common health problem, especially in developing countries like Nigeria. The current cascade of antifungals are either too toxic or require long term use for total eradication. This study evaluates antifungal activity and phytochemical constituents of Luffa cylindrica leaves extract which were screened for the presence of bioactive phytochemicals and extracted by cold maceration in n-hexane, ethyl acetate, methanol and water. The ethyl acetate extract was further fractionated using bio-assay guided column chromatography. In vitro antifungal activities were investigated against three types of fungi which were, Candida albicans ATCC 2876, Candida tropicalis ATCC 19092, Trichophyton rubrum ATCC 28188; and four clinical isolates of C. albicans, C. tropicalis, Microsporum canis and Epidermophyton flocossum using agar diffusion and micro broth dilution methods. The crude extracts revealed the presence of sterols, saponins, flavonoids, phenols and alkaloids. The ethyl acetate extract produced the strongest antifungal activity with diameter zones of inhibition ranging from 13.00 to 16.00 mm at an exposure concentration of 2500 µg/mL. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values of the ethyl acetate extract against the test fungi were 500 to 1000 µg/mL and 2000 to 4000 µg/mL, respectively. L5 and L6 fractions produced fungal inhibitory activity comparable to the crude ethyl acetate extracts of L. cylindrica with MIC values of 1000 to 2000 µg/mL and 500 to 2000 µg/mL, respectively. The ethyl acetate extracts of L. cylindrica possess antifungal properties that could serve as leads for the development of novel antifungal drugs.

 

Key words: Luffa cylindrica leaves, antifungal, phytochemical screening, minimum inhibitory concentration.

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APA Aboh, M. I., Oladosu, P., Adeshina, G., Olayinka, B., & Olonitola, S. O. (2017). Phytochemical screening and antifungal activity of leaves extracts of Luffa cylindrica (Roem). African Journal of Microbiology Research, 11(47), 1681-1687.
Chicago Mercy Itohan Aboh, Peters Oladosu, Gbonbola Adeshina, Busayo Olayinka and Stephen Olayeni Olonitola. "Phytochemical screening and antifungal activity of leaves extracts of Luffa cylindrica (Roem)." African Journal of Microbiology Research 11, no. 47 (2017): 1681-1687.
MLA Mercy Itohan Aboh, et al. "Phytochemical screening and antifungal activity of leaves extracts of Luffa cylindrica (Roem)." African Journal of Microbiology Research 11.47 (2017): 1681-1687.
   
DOI 10.5897/AJMR2017.8715
URL http://www.academicjournals.org/journal/AJMR/article-abstract/30241CE55263

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