African Journal of Food Science
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Article Number - B3A8DFA55547


Vol.12(2), pp. 15-20 , February 2018
https://doi.org/10.5897/AJFS2017.1656
ISSN: 1996-0794


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Full Length Research Paper

Validation of the use of spectrophotometer (WYD iodine checker) for the determination of iodine in food salt



Carmelle Mizehoun-Adissoda
  • Carmelle Mizehoun-Adissoda
  • Faculté des Sciences de Santé de Cotonou, Université d'Abomey Calavi, FSS/UAC, 01 BP 188 Cotonou, Bénin.
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Victoire Agueh
  • Victoire Agueh
  • Department of Health Promotion, Regional Institute of Public Health, 01 BP 918 Ouidah, Benin.
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Achille Yemoa
  • Achille Yemoa
  • Laboratoire de Chimie Analytique et Analyse des Médicaments (LCAM), Faculté des Sciences de Santé de Cotonou, Université d'Abomey Calavi, 01 BP 494 Cotonou, Bénin.
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Boris Segla
  • Boris Segla
  • Faculté des Sciences de Santé de Cotonou, Université d'Abomey Calavi, FSS/UAC, 01 BP 188 Cotonou, Bénin.
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Florence Alihonou
  • Florence Alihonou
  • Department of Health Promotion, Regional Institute of Public Health, 01 BP 918 Ouidah, Benin.
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Roger Gerard Josse
  • Roger Gerard Josse
  • Laboratoire de Chimie Analytique et Analyse des Médicaments (LCAM), Faculté des Sciences de Santé de Cotonou, Université d'Abomey Calavi, 01 BP 494 Cotonou, Bénin.
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Dismand Houinato
  • Dismand Houinato
  • Laboratory of Non-Communicable and Neurologic Diseases Epidemiology (LEMACEN), Faculty of Health Science, University of Abomey-Calavi, Cotonou, Benin.
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Jean-Claude Desport
  • Jean-Claude Desport
  • CHU Limoges, Unit of Nutrition, Limoges, France.
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Andre Bigot
  • Andre Bigot
  • Faculté des Sciences de Santé de Cotonou, Université d'Abomey Calavi, FSS/UAC, 01 BP 188 Cotonou, Bénin.
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 Received: 07 September 2017  Accepted: 19 December 2017  Published: 28 February 2018

Copyright © 2018 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The iodine content in food salt is generally determined qualitatively using rapid test kits or quantitatively by iodometric titration (reference method). Spectrophotometric analysis is one of the recent developed quantitative methods, which has the advantage of being simple, robust and more convenient for the laboratory technician. However, there are few comparative studies between this method and the reference method. The aim of this study was to evaluate the agreement between spectrophotometric and iodometric titration methods. From May to October 2013, 117 salt samples were collected in the households of Glazoué’s town (Benin), through a three-stage sampling. Samples were assayed by iodometric titration and by a portable spectrophotometer (WYD Iodine Checker). The agreement between results of the two methods was performed using Bland-Altman plots. The mean levels of iodine in salt samples were 28.2±14.0 and 28.4±14.0 ppm by iodometric titration and spectrophotometry respectively. There is an excellent correlation between the results of both methods (r= 0.97, p< 0.001). The agreement between the two methods gave a mean difference of d= 0.2 ppm, within the limits: d ± 2 sd= -6.2 and 6.5 ppm. This study showed that the spectrophotometric method can replace the iodometric titration for iodine analysis in dietary salt. This method is more convenient, uses simple laboratory procedures and can be popularized.

 

Key words: Spectrophotometry, Iodometric titration, food salt, Iodine.

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APA Mizehoun-Adissoda, C., Agueh, V., Yemoa, A., Segla, B., Alihonou, F., Jossè, R. G., Houinato, D., Desport, J., & Bigot, A. (2018). Validation of the use of spectrophotometer (WYD iodine checker) for the determination of iodine in food salt. African Journal of Food Science, 12(2), 15-20.
Chicago Carmelle Mizehoun-Adissoda,, Victoire Agueh, Achille Yemoa, Boris Segla, Florence Alihonou, Roger G&erard Jossè, Dismand Houinato, Jean-Claude Desport, and Andr&e Bigot. "Validation of the use of spectrophotometer (WYD iodine checker) for the determination of iodine in food salt." African Journal of Food Science 12, no. 2 (2018): 15-20.
MLA Carmelle Mizehoun-Adissoda, et al. "Validation of the use of spectrophotometer (WYD iodine checker) for the determination of iodine in food salt." African Journal of Food Science 12.2 (2018): 15-20.
   
DOI https://doi.org/10.5897/AJFS2017.1656
URL http://www.academicjournals.org/journal/AJFS/article-abstract/B3A8DFA55547

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