African Journal of Environmental Science and Technology
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Article Number - D4258C355970


Vol.12(3), pp. 115-122 , March 2018
https://doi.org/10.5897/AJEST2017.2447
ISSN: 1996-0786


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Full Length Research Paper

Genotoxicity assessment of three industrial effluents using the Allium cepa bioassay



Olusola O. Ibeh
  • Olusola O. Ibeh
  • Genetics and Molecular Biology Unit, Department of Animal and Environmental Biology, Abia State University, Uturu, Nigeria.
  • Google Scholar
Solomon N. Umeham
  • Solomon N. Umeham
  • Hydrobiology and Fisheries Unit, Department of Animal and Environmental Biology, Abia State University, Uturu, Nigeria.
  • Google Scholar







 Received: 30 October 2017  Accepted: 01 January 2018  Published: 31 March 2018

Copyright © 2018 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The Allium cepa assay was employed, in conjunction with physico-chemical analysis, to investigate the potential cytotoxicity and genotoxicity of three industrial effluents (soap, beverage and paint) from the southeast of Nigeria. For in situ monitoring of cytotoxicity level, inhibition of mitotic division was investigated and for genotoxicity evaluation, chromosomal aberration assay was carried out. The results showed certain sample-constituents of the wastewaters (e.g. pH, turbidity) to be at concentrations beyond the maximum permissible limits required by international regulatory authorities. On the basis of the 72 h effective concentration (72 h EC50), the paint effluent was the most toxic while the beverage effluent was the least toxic. The mean root lengths of A. cepa exposed to different concentrations of the industrial effluents, when compared to the control, were shown by Analysis of Variance (ANOVA) to be significantly (p<0.05) concentration dependent. The three industrial effluents were observed to induce chromosomal aberrations, laggards and sticky chromosomes being the most frequently seen. The findings show that a combination of physico-chemical analysis and genotoxicity assay is effective in assessing industrial effluents for the environmental monitoring of pollutants.

Key words: Allium cepa, industrial effluents, soap, beverage, paint, genotoxicity

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APA Ibeh, O. O., & Umeham, S. N. (2018). Genotoxicity assessment of three industrial effluents using the Allium cepa bioassay. African Journal of Environmental Science and Technology , 12(3), 115-122.
Chicago Olusola O. Ibeh and Solomon N. Umeham. "Genotoxicity assessment of three industrial effluents using the Allium cepa bioassay." African Journal of Environmental Science and Technology 12, no. 3 (2018): 115-122.
MLA Olusola O. Ibeh and Solomon N. Umeham. "Genotoxicity assessment of three industrial effluents using the Allium cepa bioassay." African Journal of Environmental Science and Technology 12.3 (2018): 115-122.
   
DOI https://doi.org/10.5897/AJEST2017.2447
URL http://www.academicjournals.org/journal/AJEST/article-abstract/D4258C355970

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