African Journal of Biochemistry Research
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Article Number - E8B891356840


Vol.10(1), pp. 7-11 , January 2016
https://doi.org/10.5897/AJBR2015.0830
ISSN: 1996-0778


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Full Length Research Paper

Modulation in membrane lipids of Escherichia coli and Staphylococcus aureus in response to varying NaCl concentrations



Omotoyinbo Oluwasegun Victor
  • Omotoyinbo Oluwasegun Victor
  • Department of Biochemistry, Federal University of Technology, Akure, Ondo State, 340252, Nigeria.
  • Google Scholar
Sanni David Morakinyo*
  • Sanni David Morakinyo*
  • Department of Biochemistry, Federal University of Technology, Akure, Ondo State, 340252, Nigeria.
  • Google Scholar
Olawoye Theophilus
  • Olawoye Theophilus
  • Department of Biochemistry, Federal University of Technology, Akure, Ondo State, 340252, Nigeria.
  • Google Scholar







 Received: 23 March 2015  Accepted: 20 August 2015  Published: 30 January 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The effect of varying NaCl concentrations on membrane lipids of Escherichia coli and Staphylococcus aureus cultivated at 37°C was studied. Lipid profiles of the membrane extracts were resolved by thin layer chromatography (TLC) using polar and neutral lipids solvent systems, with their fatty acid constituents determined via gas chromatography-mass spectrometry (GC-MS). Polar lipids identified were cardiolipin, phosphatidic acid, phosphatidylglycerol, phosphatidylethanolamine and lysophosphati-dylglycerol, while neutral lipids for both microorganisms were steryl-esters, triacylglycerols, diacylglycerols and monoacylglycerols at the different NaCl concentrations of growth however triacylglycerol was not detected in S. aureus at 3% NaCl. Fatty acids for E. coli were C15:0, C16:0 and C18:1 at 0% NaCl but C16:2 was also detected at 3% NaCl. S. aureus fatty acids were C13:0, C14:0, C16:0 and C18:1 for 0% NaCl while at 3% NaCl, additional fatty acids observed include C12:0, C15:0 and C16:1. The lipids expressed were relatively similar; however, their fatty acids differed in constituents and abundance.
 
Key words: NaCl concentration, lipid profiles, fatty acids, membranes, Escherichia coli, Staphylococcus aureus.
 

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APA Omotoyinbo, O. V., Morakinyo, S. D., & Olawoye, T. (2016). Modulation in membrane lipids of Escherichia coli and Staphylococcus aureus in response to varying NaCl concentrations. African Journal of Biochemistry Research , 10(1), 7-11.
Chicago Omotoyinbo Oluwasegun Victor, Sanni David Morakinyo and Olawoye Theophilus. "Modulation in membrane lipids of Escherichia coli and Staphylococcus aureus in response to varying NaCl concentrations." African Journal of Biochemistry Research 10, no. 1 (2016): 7-11.
MLA Omotoyinbo Oluwasegun Victor, Sanni David Morakinyo and Olawoye Theophilus. "Modulation in membrane lipids of Escherichia coli and Staphylococcus aureus in response to varying NaCl concentrations." African Journal of Biochemistry Research 10.1 (2016): 7-11.
   
DOI https://doi.org/10.5897/AJBR2015.0830
URL http://www.academicjournals.org/journal/AJBR/article-abstract/E8B891356840

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