African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 369

Full Length Research Paper

Optimization of amylase production by Aspergillus niger cultivated on yam peels in solid state fermentation using response surface methodology

Samuel Kwatia
  • Samuel Kwatia
  • Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
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Victoria Pearl Dzogbefia
  • Victoria Pearl Dzogbefia
  • Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
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Isaac William Ofosu
  • Isaac William Ofosu
  • Department of Food Science and Technology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
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  •  Received: 17 April 2017
  •  Accepted: 12 June 2017
  •  Published: 31 July 2017

Abstract

The study involved the production of amylase from Aspergillus niger grown on yam peels in solid state fermentation. The process parameters: temperature, pH (initial) and incubation time were optimized for maximum amylase production using central composite design (CCD) of response surface methodology (RSM). Temperature was the most significant (p<0.05) parameter and the maximum interaction occurred between temperature and incubation time. The results of the study indicated that amylase is maximized (30.95 U/ml-min) at optimized levels of 49.53°C, 5.95 and 104 h for temperature, pH (initial) and incubation periods, respectively.

 

Key words: Response surface methodology (RSM), Aspergillus niger, central composite design, solid state fermentation (ssf), amylase, production, optimization.