November 2016
Characterization of mutant cowpea [Vigna unguiculata (L) Walp] lines using random amplified polymorphic DNAs (RAPDs) and amplified fragment length polymorphism (AFLP) markers
Phylogenetic relationship and polymorphism was detected in 10 cowpea lines comprising of leaf, flower and stem mutants, their putative parents and an exotic accession using 10 random amplified polymorphic DNAs (RAPDs) and three primer combinations of amplified fragment length polymorphism (AFLP) markers. These mutants were earlier obtained through the probable activities of transposable elements. The RAPD and AFLP...
November 2016
Pan-genome analysis of Senegalese and Gambian strains of Bacillus anthracis
Bacillus anthracis is the causative agent of anthrax, and it is classified as “category A” biological weapon. There were six available complete genomes (A0248, Ames, Ames Ancestor, CDC684, H0491 and Sterne). Here, one Gambian and two Senegalese strains (Gmb1, Sen2Col2 and Sen3) were added. In this work, the pan-genome of B. anthracis was studied based on nine strains and using bioinformatics tools as Cluster...
November 2016
RNAi-based silencing of genes encoding the vacuolar-ATPase subunits a and c in pink bollworm (Pectinophora gossypiella)
RNA interference is a post- transcriptional gene regulation mechanism that is predominantly found in eukaryotic organisms. RNAi demonstrated a successful gene silencing in insects that led to the development of novel approaches for insect pest management. In the current study, genes encoding vacuolar ATPase (V-ATPase) subunits a and c from the midgut of pink bollworm, Pectinophora gossypiella, were cloned and sequenced....
November 2016
Genetic analysis of body weight of Takifugu rubripes at different developmental stages
To elucidate the genetic mechanism of growth trait in Takifugu rubripes during ontogeny, developmental genetic analysis of body weight was conducted by mixed genetic model with additive-dominance effects, using complete diallel cross with three different strains of T. rubripes from Laizhou Shandong, Tangshan Hebei and Dalian Liaoning. Unconditional genetic analysis revealed that the unconditional dominance effects with...
November 2016
Diversity of growth hormone gene and its relation with average daily gain in Simmental cattle in West Sumatera Province, Indonesia
This study was aimed to analyse the genetic polymorphism of Growth Hormone (GH) polymorphism of Simmental cattle using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and its relation to average daily gain. The research was conducted in the Padang Mangatas Breeding Centre, Limapuluh Kota district, West Sumatera Province and Biotechnology Laboratory of Faculty of Animal Husbandry,...
November 2016
Hesperidin effects on behavior and locomotor activity of diabetic Wistar rat
Today, there are sufficient validated scientific data that support the existence of relations between diabetes and certain neuropsychiatric disorders, such as behavioral disorders, anxiety, cognitive decline and depression. The objective of this work was to investigate the effect of a natural bioflavonoid, the antioxidant hesperidin on the neuro behavioral alterations and locomotor function in streptozotocine diabetic...
November 2016
Optimization of humidifying procedure in controlled environment for indoor cultivation of Pleurotus pulmonarius
This study investigated mushroom cultivation in controlled indoor environment. For this, the environmental factors including humidity and temperature were controlled by humidifier and ventilation. Four ventilations were installed on tops to bring the air from inside to outside and four small ventilations were installed on side wall along with black filter to bring the air from outside to inside. Four humidifiers...
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