December 2014

Cloning of nis gene and Nisin purification from Lactococcus lactis subsp. lactis Fc2

The present study was focused on nisin produced by Lactococcus lactis subsp. lactis (Fc2 isolate).  The isolate was identified by 16S rRNA using specific universal primer. The obtained cell-free supernatant inhibits the growth of Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Bacillus subtilis by using agar diffusion assay. Nisin structural gene was...

Author(s): D. E. El-hadedy and Eman W. El-gammal

December 2014

Promoting increased Chlorella sorokiniana Shih. et Krauss (Chlorophyta) biomass production using Moringa oleifera Lam. leaf extracts

Chlorella sorokiniana Shih. et Krauss, a unicellular green alga was assayed to assess its to promotion potentials response of aqueous and ethanolic leaf extracts of Moringa oleifera Lam. C. sorokiniana grown in 200 ml aliquots of modified basal medium for two weeks: was treated with the aqueous and ethanolic extracts at 10, 20, 30, 40 and 50% concentrations, respectively. A control was set up without Moringa extract and...

Author(s): NWEZE Nkechinyere Onyekwere and NWAFOR Felix Ifeanyi

December 2014

Phytoextraction potential of cadmium and lead contamination using Melia azedarach and Populus alba seedlings

Vegetative growth, biomass, chemical content and uptake of cadmium (Cd) and lead (Pb) in Melia azedarach L. (chinaberry) and Populus alba L. (white poplar) seedlings were investigated using a 2-year pot experiment. The results indicated that P. alba and M. azedarach are tolerant to contaminated soil by Cd or Pb without any toxicity symptoms. Vegetative growth and chemical properties of M. azedarach are negatively...

Author(s): Khamis, M. H., El-Mahrook, E. M. and Abdelgawad, M. A.

December 2014

Karyotype evolution and species differentiation in the genus Rattus of Manipur, India

Rattus is the most studied genus all over the world but species of the genus are not thoroughly reported from Manipur. The present paper deals with the morphometric, cytotaxonomic and phylogenetic studies of Manipur, India. The different species of Rattus namely Rattus rattus, Rattus brunneusculus, Rattus tanezumi and Rattus nitidus were studied. Berylmys manipulus and Berylmys mackenziei were taken from the genus...

Author(s): Chingangbam Dh., J. M. Laishram, Naorem Brajendra Singh, Loidang Taibangjam and Chingakham Brajakishore

December 2014

Quantification of the gene expression of bell peppers (Capsicum annuum) ripening gene(s) using real -time PCR

Fruits can be divided into two groups according to the regulatory mechanisms underlying their ripening process. The two ripening processes are climacteric and non-climacteric process; bell peppers are part of the non-climacteric plant groups. Bell peppers are members of the Solanacaea family. The Solanacaea family is best known for its fruits around the world. Today’s main focus is targeted to fruit ripening, in...

Author(s): Osama S. Hassan, Fatma Badie and Gehan Safwat

December 2014

Hydrogen 1 (1H) magnetic resonance spectroscopy (MRS) of 3.0T in assessment of steatosis to antiviral therapy for chronic hepatitis C

This study investigates the utility of Hydrogen 1 (1H) magnetic resonance spectroscopy as a noninvasive test for steatosis of response to interferon and ribavirin treatment in patients developing different severity of hepatitis C virus (HCV). Ninety chronic hepatitis C patients undergoing antiviral therapy with interferon and ribavirin underwent 1H MR spectroscopy at 3.0T before treatment, 6 month after the start of...

Author(s): Qian Zhang#,Yonggui Zhang#, Chunyu Zhang, Ping Zhao, Jian Jiao and Jiangbin Wang

December 2014

Determination of aflatoxin B1 in food products in Thailand

Aflatoxin B1 is a secondary metabolite of Aspergillus flavus and Aspergillus parasiticus. It can be formed in commodities before and after harvest. This mycotoxin possesses a variety of toxic effects, potent carcinogen to both animal and human health. Aflatoxin B1 is generally found in feed and food stuff, such as cereal and all products derived from cereals, including processed cereals since it has been proven to be at...

Author(s): K. Charoenpornsook and P. Kavisarasai

December 2014

Callus induction and RAPD analysis of Simarouba glauca DC

Callus induction for somatic embryogenesis from Simarouba glauca DC leaf explants of three genotypes (S. glauca 5, S. glauca 19 and S. glauca 21) was studied. Leaf explants (leaf segments from basal, middle and tip of the leaves) were cultured on two types of nutrient media; SGC1 and SGC2. Both media contained Murashige and Skoog (MS) medium with vitamins: 100 mg/L ascorbic acid, 0.5 mg/L 6-benzylaminopurine (BAP), 0.5...

Author(s): Jyothi Kakuturu , Josekutty PC, Shobha Potlakayala, Mathew Reitzel, Kinza Salim, Subhashini Charyulu, Rafiyat Adeyiga , Shyam Menon, Stephen L. Goldman , Pooja Patel, Michael J. Chorney ,  and Sairam Rudrabhatla

December 2014

Identifying salt stress-responsive transcripts from Roselle (Hibiscus sabdariffa L.) roots by differential display

No previous study has been reported on the salt-modulated gene(s) of roselle (Hibiscus sabdariffa L.). Identifying the potentially novel transcripts responsible for salt stress tolerance in roselle will increase knowledge of the molecular mechanism underlying salt stress responses. In this study, differential display reverse transcriptase PCR (DDRT-PCR) was used to compare the overall differences in gene expression...

Author(s): Bahaeldeen Babiker MOHAMED, Muhammad Naveed SHAHID, Bushra RASHID, Adil JAMAL, Beenish AFTAB, Abdelhafiz Adam DAHAB, Sarfraz KIANI, Muhammad Aleem ASHRAF,  Muhammad Bilal SARWAR, Sameera HASSAN and Tayyab HUSNAIN