September 2011
Molecular cloning and functional analysis of the follicle-stimulating hormone (FSH) receptor gene promoter from the Jintang black goat
A 762 bp fragment of the 5’-flanking region of the FSHR gene from the Jintang black goat was cloned. The putative initial transcript site was the A at 681 bp and there were 7 putative cis-acting elements and 3 AT-rich regions. The sequence of theFSHR promoter from the Jintang black goat is 99.34% homology to Capra hircus, 32.38% to Gallus gallus and 38.55% to mouse. It could...
September 2011
Assessment of antibiotic susceptibilities, genotypic characteristics and biofilm-forming abilities of Staphylococcus aureus and Salmonella Typhimurium
This study was designed to evaluate the antibiotic susceptibilities, genotypic characteristics and biofilm formation abilities of antibiotic-sensitive Staphylococcus aureus KACC 13236 (SAS), multiple antibiotic-resistant S. aureus CCARM 3080 (SAR), antibiotic-sensitive Salmonella Typhimurium KCCM 40253 (STS) andmultiple...
September 2011
DNA-based species identification for faecal samples: An application on the mammalian survey in Mountain Huangshan Scenic Spot
Noninvasive methods using genetic markers have been suggested as ways to overcome difficulties associated with documenting the presence of elusive species. We presented and assessed the casework of species identification based on faecal samples of terrestrial mammals in well-known Mountain Huangshan Scenic Spot. By using a pair of universal primer (L2513/H2714), a 220-bp amplicon of mitochondrial 16S rRNA...
September 2011
Production and confirmation of clones using gynogenesis in Japanese flounder
Two homozygous clones of Japanese flounder were produced by the gynogenesis that suppresses the cleavage of the eggs in the mitosis and then blocks the extrusion of the second polar body in the meiosis. Microsatellite was used to identify the genetic status of maternal parents and their progenies. 21 polymorphic microsatellite markers were chosen, distributed at the distal region of the 16 linkage group. In...
September 2011
De novo characterization of Lycoris sprengeri transcriptome using Illumina GA II
The Illumina GA II high-throughput sequencing technology was used to establish a transcriptome library from the bulbs of Lycoris sprengeri. A total of 24 million short read with an average length of 90 nt were generated, which produced 98,150 unigenes with a mean length of 385 nt. A total of 45.9% of these unigenes (45,052) were annotated using BLAST (E-value ≤1.0E-5) against the nr, SwissProt, Kyoto...
September 2011
Molecular cloning of endochitinase 33 (ECH33) gene from Trichoderma harzanium
This study was conducted to screen for the presence of ech33 gene in 80 isolates of Trichoderma. Furthermore, using gene specific primers, ech33 gene were cloned into pTZ57R/T from T. harzanium IABT1068. The clone was confirmed through PCR amplification and restriction analysis. The clones were sequenced and analyzed for homology at nucleotide and protein level to find out...
September 2011
An association study between polymorphism of alcohol dehydrogenase (ADH1B), aldehyde dehydrogenase (ALDH2), cytochrome (CYP4502E1), Catechol-O-Methyltransferase (COMT) and 5-hydroxytryptamine transporter (5-HTT) genes in Yunnan Han population with alcohol dependence
Alcohol dependence (AD) is a complex disease resulting from the inheritance of several susceptible genes and multiple environmental determinants. The aim of thisstudy was to identify the genetic risk factors which include alcohol metabolizing genes and neurotransmitter related genes for alcoholism in Yunnan Han population. Eight allelic variants of five genes were genotyped from 332 Yunnan Han...
September 2011
Synthesis and characterization of interspecific trigenomic hybrids and allohexaploids between three cultivated Brassica allotetraploids and wild species Brassica fruticulosa
Interspecific reciprocal crosses between three cultivated Brassica allotetraploidsand one wild species Brassica fruticulosa (FF, 2n =16) were made and the trigenomic hybrids were produced only with embryo rescue. From the crosses withBrassica juncea (AABB, 2n = 36) and Brassica napus (AACC, 2n = 38), hybrids (F.AB, 2n = 26; F.AC, 2n = 27) were obtained...
September 2011
High-frequency shoot regeneration of nodal explants from Tetrastigma hemsleyanum Diels et Gilg: A valuable medicinal plant
This paper describes the shoot regeneration of nodal segments from a medicinalplant, Tetrastigma hemsleyanum Diels et Gilg (Vitaceae). The highest number of shoots (7.27 shoots per explant) was observed in MS medium supplemented with 4 mg/l BA after six weeks of inoculation. 2 mg/l BA in combination with 0.1 mg/l NAA not only induced shoot proliferation...
September 2011
Effects of shading and covering material application for delaying harvest on gray mold disease severity
To delay the harvest of Sultani Cekirdeksiz grape variety and to reduce pre and post-harvest botrytis bunch rot severity, shading and covering material application were tested in 2009 to 2010 growing periods. In this study, grape vines were shaded with shading materials which had three different shading densities (35, 55, and 75% shading density) from veraison period to harvest. The grape vines were...
September 2011
Optimization of breeding methods when introducing multiple resistance genes from American to Chinese wheat
Stripe rust is one of the most destructive diseases of wheat worldwide. Growing resistant cultivars with resistance genes is the most effective method to control this disease. QuLine is a computer tool capable of defining genetic models, breeding strategies and predicting parental selection using known gene information. This paper reports the breeding process for pyramiding resistance genes to stripe rust...
September 2011
Effects of bud loading levels and nitrogen doses on yield, physical and chemical properties of brined grape-leaves
The aim of this study was to investigate the effects of several bud loading levels in winter pruning and nitrogen doses on yield and physical and chemical properties of fresh vine-leaves of grape cultivar “Narince”. Vines trained with bilateral cordon system was pruned to yield 35000 to 53000 buds/ha (16 or 24 buds/vine) above second bud in 2005 to 2006 growing season. Four nitrogen doses (0, 70,...
September 2011
Effect of root-knot nematode and two species of crown gall on antioxidant activity of grape leaves
Agrobacterium tumefaciens and Agrobacterium vitis significantly reduced the entire criteria (galls, embedded stages, final population, Pf/Pi and egg production) of the root-knot nematode, Meloidogyne incognita, when they were in concomitance with grape (Vitis vinifera var) superior roots. Greater suppression in such criteria was observed with high inoculum levels (5X107 cfu/pot) than...
September 2011
Effect of genotypes and culture medium on shoot regeneration and proliferation of Gerbera jamesonii
Gerbera jamesonii is one of the most popular cut flowers in the world and micropropagation is the commercial way for its propagation. This method allows for obtaining large amounts of healthy homogenous plants. Thus, it is necessary to establish efficient micropropagation protocols. The objective of this study was to evaluate the organogenic response of G. jamesonii, orange and pink...
September 2011
Histopathology of gill, liver, muscle and brain of Cyprinus carpio communis L. exposed to sublethal concentration of lead and cadmium
Histological studies in organs like gill, liver, muscle and brain of Cyprinus carpio communis were made to assess tissue damage due to sublethal concentration of heavy metals lead and cadmium after 28 days of exposure. In lead treated gill, disintegration and fusion of primary lamellae, extensive vacuolization with disruption of epithelial lining was observed, whereas on sublethal exposure to...
September 2011
Biotransformation of indigo carmine to isatin sulfonic acid by lyophilized mycelia from Trametes versicolor
In this work, acrylonitrile (AN) and acrylic acid (AA) monomers were directly grafted onto chitosan using ammonium persulfate (APS) as an initiator and methylenebisacrylamide (MBA) as a crosslinking agent under an inert atmosphere. The hydrogels structure was characterized by Fourier transform infrared (FTIR)spectroscopy, scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The effect of...
September 2011
Seasonal variation of mercury vapor concentrations in industrial, residential, and traffic areas of Ahvaz city, Southwest Iran
Mercury has been known as a toxic substance that could raise potential risks to human health. The main anthropogenic sources of mercury pollution in air include combustion of fossil fuel, metal smelting and processing, and vehicle transportation all of which exist in Ahvaz city in Southwestern Iran. Ambient air mercury vapor concentrations were measured in summer and winter in industrial, residential and...
September 2011
Identification of Lactobacillus pobuzihii from tungtap: A traditionally fermented fish food, and analysis of its bacteriocinogenic potential
Lactic acid bacteria (LAB) were selectively isolated from tungtap, a traditionally fermented fish food product. Five such bacteria with bacteriocinogenic potential were characterized by polyphasic taxonomic approach. The phylogenetic analyses of 16S rRNA gene sequences showed their relatedness to Lactobacillus pobuzihii(≥99.4% similarity), while biochemical and physiological characteristics revealed...
September 2011
Repeated batch production of vancomycin using synthetic cotton fibers
The production of vancomycin by free and immobilized cells of Amycolatopsis orientalis was investigated. The influence of immobilization through entrapment in Ca-alginate beads and adsorption on different materials including synthetic cotton fibers, glass wool, cotton cloth and absorbed cotton on the production was compared to free cells in shake flask cultures. Cells entrapped in Ca-alginate beads gave...
September 2011
A novel polymorphism of resistin gene and its association with meat quality traits in Chinese Bos taurus
Searching for candidate gene polymorphisms and their relationship with meat quality traits is an important issue for Bos taurus industry. In this study, we evaluated polymorphism of resistin (RETN) gene involved in energy metabolism. Using the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technology, a novel missense substitution single-nucleotide...
September 2011
An oxidant, detergent and salt stable alkaline protease from Bacillus cereus SIU1
A novel soil bacterium, Bacillus cereus SIU1 was earlier isolated from non-saline, slightly alkaline soil of Eastern Uttar Pradesh, India. The isolate B. cereus SIU1 was grown in modified glucose yeast extract (modified GYE) medium at pH 9.0 and 45°C. It produced maximum protease at 20 h incubation. The enzyme was stable at pH 9.0 and 55°C. It was fully stable at 0.0 to 3.0%...
September 2011
Synthesis and characterization of superabsorbent hydrogel based on chitosan-g-poly (acrylic acid-co-acrylonitrile)
In this work, acrylonitrile (AN) and acrylic acid (AA) monomers were directly grafted onto chitosan using ammonium persulfate (APS) as an initiator and methylenebisacrylamide (MBA) as a crosslinking agent under an inert atmosphere. The hydrogels structure was characterized by Fourier transform infrared (FTIR)spectroscopy, scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The effect...
September 2011
Phyto-agglutinin, total proteins and amino assimilating enzymatic activity of indigeneous chickpea (Cicer arietinum L.) cultivars
Biochemical studies were carried out with in vitro micropropagated plantlets of two indigenous cultivars of chickpea (Cicer arietinum L.), KK-1 and Hassan-2K, where extract from shoots, leaves, roots, and reproductive organs were determined for human erythrocyte agglutination (by mixing the lectin containing extract 1:1 with a 2% erythrocyte suspension), protein profiling and the amino...
September 2011
Computational design of disulfide cyclic peptide as potential inhibitor of complex NS2B-NS3 dengue virus protease
Development of genomic and proteomic studies coupled with computational sciences could facilitate the discovery of various target proteins and potential inhibitor to be developed as drugs. Several researches by molecular docking method have been conducted to design disulfide cyclic peptide ligand as potential inhibitors for NS2B-NS3 protease (NS2B-NS3 pro) of dengue virus serotype DENV-2 in order to...
September 2011
Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok 16424, Indonesia.
The aim of this study was to investigate the anti-inflammatory activities of tetramethylpyrazine (TMP) in the mice model of acute lung injury/acute respiratory distress syndrome (ALI/ARDS) induced by oleic acid (0.15 ml/kg, intravenous (i.v.)). We report here that TMP (given at 160 mg/kg intraperitoneal (i.p.)) exerts potent anti-inflammatory effects in this model. After 4, 8 and 12 h, animal model reaction was...
September 2011
Study on the growth and development of brinjal shoot and fruit borer with different diets
A laboratory experiment was conducted with two natural and one artificial diet on the growth and development of brinjal shoot and fruit borer (BSFB). The population of BSFB used in the study was in the 2nd instar larvae. Among the different diet, brinjal was the best for growth, development and longevity of larvae and pupae and prolongation of larval and pupal period. The mean length of full...
September 2011
Effect of carbofuran on the diversity and mean abundance of ground spiders
The effect of carbofuran on the diversity, richness, evenness and abundance of ground spiders is documented in the present study. Two fields (control and treated) were selected in district Kasur during 2008 and 2009. Pitfall traps were used to collect ground spiders. A total of 1629 specimens of spiders, belonging to 8 families, 16 genera and 24 species were recoded from control field while 1173 specimens...
September 2011
Effects of storage duration and storage temperature on viability of stored ova of kutum (Rutilus frisii kutum) in ovarian fluid
1Department of Aquaculture and Fisheries Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran. 2Department of Fisheries and Environmental Science, Faculty of Natural Resources, University of Tehran, 31585-4314, Karaj, Iran. 3Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, 14155-6453,...
September 2011
Breeding performance of sustainable fish Ctenopharyngodon idella through single intramuscular injection of Ovaprim-C at Bahawalpur, Pakistan
Effect of intramuscular injection of Ovaprim–C on the number of eggs/kg, fertilization rate and hatching percentage were studied at a private Fish Hatchery at Bahawalpur, Pakistan, during April to June 2008, on Ctenopharyngodon idella(Grass carp). Studied fish specimens were spawned successfully following a single dose of injection of Ovaprim-C (LH-RH analogue) with 0.6 ml kg-1 for female and...
September 2011
Apple and quince peroxidase activity in response to essential oils application
Enzymatic browning arises by peroxidase in fruits. However, essential oils arerecognized as natural antioxidant agents. So in this study, the effect of thyme, coriander and rosemary essential oils were evaluated on the reduction of peroxidase activity in apples (Malus domestica Mill. cv Golden delicious), (M.domestica Mill. cv Red delicious) and quince...
September 2011
Apple and quince peroxidase activity in response to essential oils application
Enzymatic browning arises by peroxidase in fruits. However, essential oils arerecognized as natural antioxidant agents. So in this study, the effect of thyme, coriander and rosemary essential oils were evaluated on the reduction of peroxidase activity in apples (Malus domestica Mill. cv Golden delicious), (M.domestica Mill. cv Red delicious) and quince...
September 2011
The effect of all-trans retinoic acid (ATRA) on the expression of vascular endothelial growth factor (VEGF) and VEGF receptors of human colon cancer LoVo cell line
All-trans retinoic acid (ATRA) was found to inhibit cell growth, induce differentiation and enhance apoptosis in a variety of malignant solid tumors. Retinoic acid is effective in inhibiting the expression of vascular endothelial growth factor (VEGF) in some cancer. In this study, we investigated the effect of ATRA on the expression of VEGF and its receptors in LoVo cells, and its possible mechanisms. LoVo...
September 2011
Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of Mycoplasma bovis
A loop-mediated isothermal amplification (LAMP) assay targeting uvrC ofMycoplasma bovis was developed and evaluated. The assay specifically amplified only M. bovis; no cross-reactivity was observed for other Mycoplasma species or respiratory bacterial species. The sensitivity of the assay in pure cultures was 10-fold higher than that of polymerase chain reaction (PCR), with a...
September 2011
Effects of early and chronic exposure to high temperatures on growth performance, carcass parameters and fatty acids of subcutaneous lipid of broilers
This study was conducted to assess the effects of early and chronic exposure of broilers at a scorching temperature on growth performance, carcass parameters, fatty acid deposition and composition of subcutaneous lipids (SCL). Three hundred male chicks (Hubbard origin) of a day are reared for 50 days. They are divided into three groups, each of cent chicks. The chronic thermal environments are applied from...
September 2011
Comparison of response to selection in three peanut cocoon lines of silkworm during spring and autumn seasons
The aim of this experiment was to study the season effect on response to selection in three Iranian silkworm pure lines. In the first year, a parental group was selected, recorded and coded for each 3 lines of 31, 103 and 107, which had further weight average than the population average. These parents were crossed with each other and a selected group was made for these three lines. Also, a parental group was...
September 2011
Effect of gonadotropin-releasing hormone (GnRH) treatment on multiple births in Afshari ewes
The purpose of this study was to investigate the effects of gonadotropin-releasing hormone (GnRH) administration on the induction of multiple births in synchronized Afshari ewes. 16 cycling, multiparous fat-tailed Iranian Afshari ewes, weighing 66.5 ± 2.5 kg, were used in the trail. Estrus was synchronized using controlled internal drug release CIDR’s; inserted for 14...
September 2011
The effects of pregnant mare serum gonadotropin (PMSG) injection a day prior or at controlled intravaginal drug-releasing (CIDR) removal on multiple births in Afshari ewes
According to the importance of lambing rate to profitability of sheep holders, this trial investigated the effects of pregnant mare serum gonadotropin (PMSG) injection a day prior or at controlled intravaginal drug-releasing device (CIDR) removal on multiple births in synchronized Afshari ewes. 16 cycling, multiparous fat-tailed Iranian Afshari ewes, weighing 66.5±2.5 kg, were used in the...
September 2011
Studies on the influence of temperature and humidity on biological traits of silkworm (Bombyx mori L.; Bombycidae)
Impact of variations in temperature and humidity on pupation, hatchability and larval mortality of eleven inbred silkworm lines M-101, M-103, M-104, M-107, Pak-1, Pak-2, Pak-3, Pak-4, PFI-I, PFI-II and S-1 was investigated during autumn and spring, 2007-2008 at Sericulture Research Laboratory, Lahore. The experiment was laid out in factorial design with replications and the data was pooled over...
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