The objective of this study was the analysis of the secondary metabolite products and evaluation of antibacterial activity of Aspergillus flavus. Bioactives are chemical compounds often referred to as secondary metabolites. Thirty one bioactive compounds were identified in the methanolic extract of A. flavus. The identification of bioactive chemical compounds is based on the peak area, retention time, molecular weight and molecular formula. Gas chromatography mass spectrometry (GC-MS) analysis of A. flavus revealed the existence of the 1,2-cis-1,5-trans-2,5-dihydroxy-4-methyl-1-(1-htdroxy-1-isopropyl)cy, 2-furancarboxaldehyde,5-methyl, 2(5H)-Furanone, 6-hydroxymethyl-5-methyl-bicyclo[3.1.0]hexan-2-one, D-glucose,6-O-α-D-galactopyranosyl, 2-(3-hydroxy-propyl)-cyclohexane-1,3-dione, 9-oxa-bicyclo[3.3.1]nonane-1,4-diol, benzenemethanol,2-(2-aminopropoxy)-3-methyl, 1,2-cyclopentanedione,3-methyl, α-D-glucopyranoside, O-α-D-glucopyranosyl-(1.fwdarw.3)-ß-D-fruc, 1-nitro-2-acetamido-1,2-dideoxy-d-mannitol, desulphosinigrin, orcinol, bicyclo[2.2.1]heptane-2-carboxylic acid isobutyl-amide, 2H-oxecin-2-one.3.4.7.8.9.10-hexahydro-4-hydroxy-10-methyl-.[4, 2H-pyran,tetrahydro-2-(12-pentadecynyloxy), maltol, 2-tridecyl-5-(acetylamino)tetrahydro-γ-pyrone, cycloundecanone , oxime, D-glucose,6-O-α-D-galactopyranosyl, 6-acetyl-ß-d-mannose, 5-hydroxymethylfurfural, 1-gala-l-ido-octonic lactone, pterin-6-carboxylic acid, uric acid, acetamide , N-methyl -N-[4-[2-acetoxymethyl-1-pyrrolidyl]-2-butynyl], l-(+)-ascorbic acid 2,6-dihexadecanoate, D-fructose , diethyl mercaptal , pentaacetate, 2-bromotetradecanoic acid, octadecanal ,2 –bromo, L-ascorbic acid, 6-octadecanoate, and 18,19-secoyohimban-19- oic acid,16,17,20,21-tetradehydro-16. The FTIR analysis of A. flavus proved the presence of aromatic rings, alkenes, aliphatic fluoro compounds, aromatic nitro compounds, ammonium ions and organic nitrate which shows major peaks at 667.37, 694.37, 904.61, 1028.06, 1145.43, 1205.51, 1234.44, 1273.02, 1311.94, 1377.17, 1413.82, 1450.47, 1492.90, 1600.92, 1629.99, 2852.72, 2922.16, 3024.38, 3271.27 and 3286.7. Datura stramonium was very highly antifungal activity (9.00±0.73) mm while Escherichia coli has maximum zone formation (7.55±0.09) mm against A. flavus.

Keywords: Aspergillus flavus, antibacterial activity, antifungal activity, Fourier transform infrared spectroscopy (FTIR), gas chromatography mass spectrometry (GC/MS), secondary metabolites.