African Journal of Biotechnology
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Article Number - EEE0ADF11764


Vol.4(11), pp. 1269-1274 , November 2005
DOI: 10.5897/AJB2005.000-3250
ISSN: 1684-5315



Full Length Research Paper

Species and gender differentiation between and among domestic and wild animals using mitochondrial and sex-linked DNA markers


Allen Malisa1,3*, Paul Gwakisa2, Sakurani Balthazary1,  Sam Wasser4 and Benezeth Mutayoba1




1Department of Veterinary Physiology, Biochemistry, Pharmacology and Toxicology, 2Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, 3Department of Biological Sciences, Faculty of Science, Sokoine University of Agriculture, P.O. Box 3000, Morogoro, Tanzania.

4Center for Conservation Biology, Department of Zoology, University of Washington, Box 351800, Seattle, WA 98195-1800, USA.


Email: malisa56@yahoo.com






 Accepted: 16 September 2005  Published: 30 November 2005

Copyright © 2005 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


In many African countries accurate and reliable identification of poached wildlife products like carcasses or meat presents a big problem when morphological characters such as skin hair or bones are missing. We describe a molecular based approach that has a potential of serving as a forensic tool in game meat identification in Africa. A mitochondial DNA marker (mt700) and one restriction enzyme, Rsa1 were used in the PCR-RFLP species identification of game meat obtained from two National Parks in Tanzania. Species-specific reference DNA fragment patterns were obtained using fresh meat from ten wildlife and four domesticated species. All species except the zebra, produced unique monomorphic RFLP patterns. Collectively, these patterns demonstrate the potential ability of genetic techniques for discriminating between and among wildlife and domestic species. The reference PCR-RFLP fragments enabled species identification of about 79% of unknown meat samples. In addition, sex was also assigned to all of the samples following successful amplification of gender-specific, SRY and ZFY/X, chromosomal domains. Although the present study has been conducted on a limited range both in numbers and genetic diversity of wildlife species present in Africa, the results demonstrate the potential usefulness of the DNA approach in wildlife forensics in the continent.

 

Key words: Mitochondrial DNA, PCR, poaching, forensic, gender, species identification.


APA Malisa, A., Gwakisa, P., Balthazary, S., Wasser, S., & Mutayoba, B. (2005). Species and gender differentiation between and among domestic and wild animals using mitochondrial and sex-linked DNA markers. African Journal of Biotechnology , 4(11), 1269-1274.
Chicago Allen Malisa, Paul Gwakisa, Sakurani Balthazary,  Sam Wasser and Benezeth Mutayoba. "Species and gender differentiation between and among domestic and wild animals using mitochondrial and sex-linked DNA markers." African Journal of Biotechnology 4, no. 11 (2005): 1269-1274.
MLA Allen Malisa, et al. "Species and gender differentiation between and among domestic and wild animals using mitochondrial and sex-linked DNA markers." African Journal of Biotechnology 4.11 (2005): 1269-1274.
   
DOI 10.5897/AJB2005.000-3250
URL http://www.academicjournals.org/journal/AJB/article-abstract/EEE0ADF11764

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