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Article Number - ADD838A65905


Vol.16(36), pp. 1840-1848 , September 2017
https://doi.org/10.5897/AJB2017.16070
ISSN: 1684-5315


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Full Length Research Paper

Expression and functional evaluation of Mytilus galloprovincialis foot protein type 5 (Mgfp-5), the recombinant mussel adhesive protein



Yawei Lv
  • Yawei Lv
  • Key Laboratory of Biotechnology, China College of Life Science, Northwest University, Shaanxi, 710069, Xiʼan, China.
  • Google Scholar
Yujing Zhang
  • Yujing Zhang
  • Key Laboratory of Biotechnology, China College of Life Science, Northwest University, Shaanxi, 710069, Xiʼan, China.
  • Google Scholar
Wenying Gao
  • Wenying Gao
  • Key Laboratory of Biotechnology, China College of Life Science, Northwest University, Shaanxi, 710069, Xiʼan, China.
  • Google Scholar
Yingjuan Wang
  • Yingjuan Wang
  • Key Laboratory of Biotechnology, China College of Life Science, Northwest University, Shaanxi, 710069, Xiʼan, China.
  • Google Scholar







 Received: 14 May 2017  Accepted: 24 August 2017  Published: 06 September 2017

Copyright © 2017 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Mussel contains a variety of adhesion proteins, among which, Mytilus galloprovincialis foot protein type 5 (Mgfp-5) is one of the major proteins required for substrate adhesion. The labor-intensive nature and insufficiency of the extraction process have frequently resulted in very little purified recombinant Mgfp-5. These prompt technologies such as chemical synthesis and genetic engineering are employed to overcome these limitations. In this study, successful expression and purification of the recombinant Mgfp-5 using Escherichia coli BL21 (DE3) and affinity chromatography were reported. Production yield of 12.25% and purity of 96.92%, respectively were observed. The 3,4-dihydroxyphenylalanine (DOPA) content (9.60 pmol/g) and the adhesion (1 116 nN) in modified recombinant Mgfp-5 were 9.32 times and 1.6 times as great as those in the unmodified recombinant Mgfp-5, respectively. Recombinant Mgfp-5 at a concentration of 9.6 mg/L had little cytotoxicity on mouse L-929 fibroblast cells, which was toxic at first in cytotoxicity test, and a concentration of not more than 20 μg/mL would not lead to hemolysis of rabbit erythrocytes. In this case, recombinant Mgfp-5 is biosecure, providing the foundation for Mgfp-5 manufacturing as well as the development of clinical biological adhesive.

 

Key words: Mgfp-5, 3,4-dihydroxyphenylalanine (DOPA), adhesion, cytotoxicity, hemolysis.

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APA Lv, Y., Zhang, Y., Gao, W., & Wang, Y. (2017). Expression and functional evaluation of Mytilus galloprovincialis foot protein type 5 (Mgfp-5), the recombinant mussel adhesive protein. African Journal of Biotechnology , 16(36), 1840-1848.
Chicago Yawei Lv, Yujing Zhang, Wenying Gao and Yingjuan Wang. "Expression and functional evaluation of Mytilus galloprovincialis foot protein type 5 (Mgfp-5), the recombinant mussel adhesive protein." African Journal of Biotechnology 16, no. 36 (2017): 1840-1848.
MLA Yawei Lv, et al. "Expression and functional evaluation of Mytilus galloprovincialis foot protein type 5 (Mgfp-5), the recombinant mussel adhesive protein." African Journal of Biotechnology 16.36 (2017): 1840-1848.
   
DOI https://doi.org/10.5897/AJB2017.16070
URL http://www.academicjournals.org/journal/AJB/article-abstract/ADD838A65905

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