African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12049

Full Length Research Paper

Polymerase chain reaction amplification of 16S rDNA from three nosocomial bacterial isolates in Kaduna State, Northern Nigeria

Richard, R.
  • Richard, R.
  • Department of Biological Science, Faculty of Science, Federal University Gashua, P.M.B 1005, Yobe State, Nigeria.
  • Google Scholar
Denwe, S. D.
  • Denwe, S. D.
  • Department of Biological Science, Faculty of Science, Nigerian Defence Academy, Kaduna State, Nigeria.
  • Google Scholar
Yerima, M. B.
  • Yerima, M. B.
  • Department of Microbiology and Biotechnology, Faculty of Science, Federal University Dutse, Jigawa State, Nigeria.
  • Google Scholar
Olatunde, S. K.
  • Olatunde, S. K.
  • Department of Pure and Applied Biology, Faculty of Pure and Applied Sciences, Ladoke Akintola University of Technology, P. M. B. 4000 Ogbomoso, Oyo State, Nigeria.
  • Google Scholar


  •  Received: 15 March 2017
  •  Accepted: 13 July 2017
  •  Published: 14 February 2018

Abstract

A wide variety of opportunistic pathogens has been detected in hospital surfaces. Medical center surfaces can serve as reservoirs of pathogenic bacteria. Among this pathogens, Pseudomonas species are one of the leading causes of nosocomial infections, frequently found in hospital environments. Polymerase chain amplification system remains one of the best methods for the rapid detection of low numbers of pathogenic bacteria with core references to nucleic Acid content. However, there is a limited researches focusing on these techniques to examine the molecular content of nosocomial bacteria. The present investigation examines types and strains of bacteria present in indoor air of wards, fomites and surgical tools of three prominent hospitals namely, Ahmadu Bello University Teaching Hospital Zaria, Hajiya Gambo Sawaba Hospital Zaria City and Barau Dikko Teaching Hospital Kaduna using standard methods. Preliminary Grams reaction and biochemical characterization was done according to standard methods, DNA extraction precede PCR amplification, probable organisms include Pseudomonas aeruginosa, Corynebacterium sp., P. aeruginosa, Bacillus sp., Klebsiella pneumonia, Neisseria sp., Staphylococcus aureus and Staphylococcus epidermidis. Out of all the isolates that were of public health concern, Neisseria sp., S. aureus and P. aeruginosa are the most prevailing isolates. A strain of P. aeruginosa was observed to give a DNA sequence. P. aeruginosa was the bacteria isolates sequenced and it showed 100% similarity having the id query: 86603, when blast using National Center for Biotechnology Information (NCBI). In general, patterns were specific at either the genus level or the species level. This research has been able to show that PCR is a promising fast method for the identification of nosocomial microorganisms.

Key words: Polymerase chain reaction, nosocomial bacterial, hospital and DNA.