African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12480

Full Length Research Paper

Genetic variability of Artemisia capillaris (Wormwood capillary) by random amplified polymorphic DNA (RAPD) in Terengganu State, Malaysia

Sayed M. Zain Hasan, Mohammad Shafie B. Shafie* and Ramisah M. Shah
Faculty of Agrotechnology and Food Science, Universiti Malaysia Terengganu, 21030 Mengabang Telipot, Kuala Terengganu, Malaysia.
Email: [email protected]

  •  Accepted: 31 December 2008
  •  Published: 04 May 2009

Abstract

 

The genetic variability among individuals of Artemisia capillaris from state of Terengganu, Malaysia was examined by using the random amplified polymorphic DNA (RAPD) technique. The samples were collected from differences regional in Terengganu State. The genomic DNA was extracted from the samples leaves. Fifthty-seven oligonucleotide primers were screened and ten primers were selected (OPA 04, OPA 09, OPA 16, OPA 17, OPA 18, OPG 03, OPG 05, OPG 09, OPG 15 and 391) to amplify DNA from five samples of A. capillaris. A total of 335 RAPD fragments (RAPDs) with all polymorphic fragments (100%) with size ranging from 150 – 3000 bp were scored from the population. Genetic distance for samples ranges from 0.0000 to 0.2600.

 

Key words: Artemisia capillaris, genomic DNA, genetic variability, RAPD.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 8 No. 9
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES