Full Length Research Paper
Xun Wu1,6, Yinghong Liu1,6, Mengliang Tian2,4, Rongjun Chen3, Zuping Zheng6, Chuan He6, Yubi Huang1,2,4*, Junjie Zhang1,4,5, Hanmei Liu1,4,5 and Zhong Li6
1Maize Research Institute, Sichuan Agricultural University, Ya’an, Si Chuan 625014, China.
2Agronomy College of Sichuan Agricultural University, Ya’an, Sichuan 625014, China.
3Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha, Hunan 410125, China.
4Key Laboratory of China Education Ministry for Crop Gene Resource and Genetic Improvement, Ya’an, Sichuan, 625014, China.
5College of Life Science, Sichuan Agricultural University, Ya’an, Sichuan 625014, China.
6Nanchong Institute of Agricultural Sciences, Nanchong, Sichuan 637000, China.
A growth system was developed where N was the only growth-limiting factor. Whole-genome transcription profiling of leaf tissues were analyzed using the Affymetrix GeneChip. 129 clones showed significant change and 83 clones were classed accurately. Most induced clones were largely involved in various metabolism processes including physiological process, organelle regulation of biological process, nutrient reservoir activity, transcription regulator activity and multicellular organismal process. Putative high affinity nitrate transporter (nrt2.1) showed significant up-regulation under the severe low chronic nitrogen stress condition. Analysis of genes expression revealed several previously unidentified genes, including beta-D-glucosidase precursor gene (glu2), and Cyc3 cyclin3 gene.It suggests that under the said condition, nrt2.1 plays the most important role in N absorption and most of the other genes induced expression to enable the maize to have normal growth. A better understanding of the complex regulatory network for plant N responses among these genes will help and lead to improve N use efficiency.
Key words: Genomics analysis, genes expressed, low chronic nitrogen stress, maize.Abbreviation:
ATH1, Arabidopsis thaliana homeobox gene 1; NR, nitrate reductase; NiR, nitrite reductase; GS, glutamine synthetase; RT-PCR, reverse transcription-polymerase chain reaction; qRT-PCR, quantitative RT-PCR; FDR,false discovery rate; ELIP, light-induced proteins;
|APA||(2011). Genomics analysis of genes expressed reveals differential responses to low chronic nitrogen stress in maize. African Journal of Biotechnology , 10(6), 939-948.|
|Chicago||Xun Wu, Yinghong Liu, Mengliang Tian, Rongjun Chen, Zuping Zheng, Chuan He, Yubi Huang,, Junjie Zhang,, Hanmei Liu, and Zhong Li. "Genomics analysis of genes expressed reveals differential responses to low chronic nitrogen stress in maize." African Journal of Biotechnology 10, no. 6 (2011): 939-948.|
|MLA||Xun Wu, et al. "Genomics analysis of genes expressed reveals differential responses to low chronic nitrogen stress in maize." African Journal of Biotechnology 10.6 (2011): 939-948.|