African Journal of Biotechnology
Vol.10(14) , pp. 2638-2643 , April 2011
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Full Length Research Paper
Morphological and molecular characterization of Oyster mushroom (Pleurotus spp.)
Samiya Mahmood Khan1, Aamir Nawaz2, Waqas Malik3*, Nazir Javed4, Tahira Yasmin5, Mehboob ur Rehman8, Abdul Qayyum3, Qumer Iqbal6, Tanveer Ahmad6 and Azhar Ali Khan7
1Department of Plant Pathology, University College of Agriculture, Bahauddin Zakariya University, Multan Pakistan.
2Department of Horticulture, University College of Agriculture, Bahauddin Zakariya University, Multan Pakistan.
3Department of Plant Breeding and Genetics, University College of Agriculture, Bahauddin Zakariya University, Multan Pakistan.
4Department of Plant Pathology, University of Agriculture, Faisalabad Pakistan.
5Department of Botany, University of Agriculture, Faisalabad Pakistan.
6Institute of Horticultural Sciences, University of Agriculture, Faisalabad Pakistan.
7PARC substation, Multan, Pakistan.
8NIBGE, Faisalabad, Pakistan.
RAPD, Random amplified polymorphic DNA; PCR, polymerase chain reaction; PDA, potato dextrose agar.
Accepted: 20 January 2011 Published: 04 April 2011
Mushrooms have medicinal as well as nutritive value and extensively used as human food from the time immortal. In order to determine the genetic diversity among Pleurotus species of mushroom using morphological and random amplified polymorphic DNA (RAPD) markers, about seven different species were collected. Five species, naming Pleurotus platypus (P-6), Pleurotus flabelatus (P-7),Pleurotus florida (P-17), Pleurotus ostreatus (P-19) and Pleurotus sajor-caju (P-56) were from Canada and two Pleurotus warm-stram (P-9) and Pleurotus eryngii (P-16) from Philpines. Seven different morphological traits that is, mycelial growth (mm), cap diameter (cm), total yield (kg), moisture contents (%), ash contents (%), nitrogen contents (%) and protein content (%) were recorded. The dendrogram based on morphological data divided seven species in cluster ‘A’ and ‘B’ having four and three species, respectively. The dendrogram based on RAPD analysis generated 3 clusters ‘A’, ‘B’ and ‘C’. Out of 14 random primers, the maximum polymorphism was observed by primers OPL3 (72.70 %) and OPL11 (70%). The two species P-56 and P-17 were observed to be most similar having value 86% and constituting a cluster ‘A’. The present work revealed that, morphological and RAPD markers showed different pattern of genetic diversity among different Pleurotusspecies.
Key words: Oyster mushrooms, genetic diversity, random amplified polymorphic DNA (RAPD) markers, dendrogram.