African Journal of Biotechnology
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Article Number - 528FF4562176


Vol.15(52), pp. 2824-2847 , December 2016
DOI: 10.5897/AJB2016.15464
ISSN: 1684-5315



Full Length Research Paper

Genetic diversity and population structure of common bean (Phaseolus vulgaris L) germplasm of Ethiopia as revealed by microsatellite markers



Zelalem Fisseha
  • Zelalem Fisseha
  • Addis Ababa University, Department of Microbial, Cellular, and Molecular Biology, P.O.Box 1176, Addis Ababa, Ethiopia.
  • Google Scholar
Kassahun Tesfaye
  • Kassahun Tesfaye
  • Addis Ababa University, Department of Microbial, Cellular, and Molecular Biology, P.O.Box 1176, Addis Ababa, Ethiopia.
  • Google Scholar
Kifle Dagne
  • Kifle Dagne
  • Addis Ababa University, Department of Microbial, Cellular, and Molecular Biology, P.O.Box 1176, Addis Ababa, Ethiopia.
  • Google Scholar
Matthew W. Blair
  • Matthew W. Blair
  • Department of Agricultural and Environmental Sciences, Tennessee State University, Nashville, TN 37209, United States of America.
  • Google Scholar
Jagger Harvey
  • Jagger Harvey
  • Biosciences Eastern and Central Africa (BecA), ILRI, Nairobi, Kenya.
  • Google Scholar
Martina Kyallo
  • Martina Kyallo
  • Biosciences Eastern and Central Africa (BecA), ILRI, Nairobi, Kenya.
  • Google Scholar
Paul Gepts
  • Paul Gepts
  • Department of Plant Sciences / MS1, University of California, 1 Shields Avenue, Davis, CA 95616, USA.
  • Google Scholar







 Received: 13 May 2016  Accepted: 09 December 2016  Published: 28 December 2016

Copyright © 2016 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


The Ethiopian genetic center is considered to be one of the secondary centers of diversity for the common bean. This study was conducted to characterize the distribution of genetic diversity between and within ecological/geographical regions of Ethiopia. A germplasm sample of 116 landrace accessions was developed, which represented different common bean production ecologies and seed types common in the country. This sample was then analyzed with 24 simple sequence repeat (SSR) markers to assess the genetic diversity within and between common bean landraces, classifying them based on SSR clustering, and determining relationships between genetic and agroecological diversity. Representatives of both Andean and Mesoamerican gene pools were identified by STRUCTURE software analysis, as well as a high proportion of hybrid accessions as evidenced by a STRUCTURE K = 2 preset. At the optimum K = 5 preset value, mixed membership of Andean and Mesoamerican genotypes in some of the clusters was also seen, which supported previous findings. Cluster analyses, principal coordinate analysis, and analysis of molecular variance all indicated clustering of accessions from different collection sites, accompanied by high gene flow levels, highlighting the significant exchange of planting materials among farmers in different growing regions in the country. Values of allelic diversity were comparable to those reported in previous similar studies, showcasing the high genetic diversity in the landrace germplasm studied. Moreover, the distribution of genetic diversity across various bean-growing population groups in contrasting geographical/ecological population groups suggests elevated but underutilized potential of Ethiopian germplasm in common bean breeding. In summary, this study demonstrated the geographical, as well as gene pool diversity in common bean germplasm of Ethiopia. This substantial diversity, in turn, should be utilized in future common bean breeding and conservation endeavors in the nation.

 

Key words: Hybridity, simple sequence repeat, microsatellite, structure, seed exchange, gene flow.

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APA Fisseha, Z., Tesfaye, K., Dagne, K., Blair, M. W., Harvey, J., Kyallo, M., Gepts, P. (2016). Genetic diversity and population structure of common bean (Phaseolus vulgaris L) germplasm of Ethiopia as revealed by microsatellite markers. African Journal of Biotechnology , 15(52), 2824-2847.
Chicago Zelalem Fisseha, Kassahun Tesfaye, Kifle Dagne, Matthew W. Blair, Jagger Harvey,  Martina Kyallo, Paul Gepts. "Genetic diversity and population structure of common bean (Phaseolus vulgaris L) germplasm of Ethiopia as revealed by microsatellite markers." African Journal of Biotechnology 15, no. 52 (2016): 2824-2847.
MLA Zelalem Fisseha, et al. "Genetic diversity and population structure of common bean (Phaseolus vulgaris L) germplasm of Ethiopia as revealed by microsatellite markers." African Journal of Biotechnology 15.52 (2016): 2824-2847.
   
DOI 10.5897/AJB2016.15464
URL http://www.academicjournals.org/journal/AJB/article-abstract/528FF4562176

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