African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12036

Full Length Research Paper

Elicitation and precursor feeding influence phenolic acids composition in Vitis vinifera suspension culture

Heidi Riedel1*#, Divine N. Akumo3#, Nay Min Min Thaw Saw1, Onur Kütük1,Peter Neubauer3 and Iryna Smetanska1, 2    
1Department of Methods in Food Biotechnology, Institute of Food Technology and Food Chemistry, Berlin University of Technology, Königin-Luise Str 22, 14195 Berlin, Germany. 2Department of Plant Food Processing, Agricultural Faculty, University of Applied Science Weihenstephan-Triesdorf, Steingruber Str 2, 91746 Weidenbach, Germany. 3Laboratory of Bioprocessing Engineering, Department of Biotechnology, Berlin University of Technology, Ackerstr 71-76, 13355 Berlin, Germany.    
Email: [email protected]

  •  Accepted: 12 December 2011
  •  Published: 09 February 2012

Abstract

Plant cell cultures have been industrially used for the synthesis of secondary metabolites. Different elicitors, [jasmonic acid (JA), salicylic acid (SA) and ethephone (E)] and precursors [shicimic acid (SH) and phenylalanine (PHE)] were independently used to enhance the synthesis of phenolics in suspension cultures of Vitis viniferac.v. Muscat de Frontignan followed by chemical analysis with high-performance liquid chromatography (HPLC). Differences between the treated and untreated in vitro grape cultures were confirmed statistically. On day 2, JA, SA and SH significantly increased phenolic acid contents with E on day 4 (p < 0.05). The grape cells treated with SA, E, SH, and PHE increased biomass whereas JA led to decreases over time. From the chemical analysis of V. vinifera suspension cell cultures, two major resveratrol derivatives; 3-O-glucosyl-resveratrol and 4-(3,5-dihydroxyphenyl)-phenol were in high concentrations after treatment most especially with JA. These results show that elicitors (JA, SA, and E) and the precursor SH can be economically used to enhance the synthesis of phenolic compounds in V. vinifera within a very short time lapse. Furthermore, these enhancers could be used to target the rapid extraction of medicinally important resveratrols for pharmaceutical purposes.

 

Key words: Vitis vinifera, phenolic acids, resveratrol, elicitors, precursors

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