African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12479

Full Length Research Paper

Identification of intergeneric hybrids between Saccharum spp. and Erianthus fulvus with ITSs

H. Y. Zhang1,2, L. L. He1, H. Q. Zhong1, F. S. Li1*, S. C. He1and Q. H. Yang1
  1College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan Province, 650201, People’s Republic of China. 2Biotechnology Laboratory, Southwest Forestry College, White Dragon Temple, Kunming, Yunnan Province, 650224, People’s Republic of China.
Email: [email protected]

  •  Accepted: 27 February 2009
  •  Published: 04 May 2009

Abstract

 

Identification of “Saccharum spp. × Erianthus fulvus” F1 hybrids was performed with ITS analysis. Out of 6 pairs of ITS primers, ZM-02 and ZM-04 were found to be suitable to detect the hybrids. The rate of true hybrids tested by ZM-02 and ZM-04 was 74.6 and 73.8% respectively and the amplification products of ZM-04 were similar to the ones of ZM-02.

 

Key words: Saccharum spp, Erianthus fulvus, intergeneric hybrids, identification, ITSs.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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