African Journal of Biotechnology
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Article Number - 0FCAD9243980


Vol.13(16), pp. 1702-1706 , April 2014
DOI: 10.5897/AJB2014.13670
ISSN: 1684-5315



Full Length Research Paper

Use of plumules cryopreservation to save coconut germplasm in areas infected by lethal yellowing



Oulo Alla-N'Nan*
  • Oulo Alla-N'Nan*
  • Université Félix Houphouet Boigny, 22 BP 582 Abidjan 22, UFR Biosciences, laboratoire de génétique, Côte d'Ivoire; IRD, UMR DIADE, 34394 Montpellier Cedex 5, France.
  • Google Scholar
Sery Gonédelé Bi
  • Sery Gonédelé Bi
  • Université Félix Houphouet Boigny, 22 BP 582 Abidjan 22, UFR Biosciences, laboratoire de génétique, Côte d'Ivoire.
  • Google Scholar
Kouakou Tiécoura
  • Kouakou Tiécoura
  • Université Félix Houphouet Boigny, 22 BP 582 Abidjan 22, UFR Biosciences, laboratoire de génétique, Côte d'Ivoire.
  • Google Scholar
Jean-Louis Konan Konan
  • Jean-Louis Konan Konan
  • Centre National de Recherche Agronomique (CNRA), Programme cocotier, Station de recherche Marc Delorme, 13 BP 989 Abidjan 13, Côte d’Ivoire.
  • Google Scholar
Bernard Malaurie
  • Bernard Malaurie
  • IRD, UMR DIADE, 34394 Montpellier Cedex 5, France.
  • Google Scholar







 Received: 28 January 2014  Accepted: 14 April 2014  Published: 16 April 2014

Copyright © 2014 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Plumules excised from zygotic embryos through the largest representative diversity of four of the five different areas of coconut cash and food crops were used in a cryopreservation process using encapsulation-dehydration technique. Five accessions of coconut trees were used [Panama Tall (PNT/GPA), Brazilian Green Tall (BGD/NVB), Cameroon Red Dwarf (CRD/NRC), Vanuatu Tall (VTT/VNT/GVT), and Tagnanan Tall (TAGT/GTN)] in addition to the accession model [Malayan Yellow Dwarf (MYD)] from which an optimal protocol was obtained. A great variability of response was observed depending on accessions with survival and growth recovery rates varying from 6 to 66% and 0 to 24% after 2 and 7 months of culture, respectively.

 

Key words: Coconut, accessions, germplasm, plumules, cryopreservation, encapsulation-dehydration.

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APA Alla-N'Nan, O., Bi, S. G., Tiécoura, K., Konan, J-L. K. & Malaurie, B. (2014). Use of plumules cryopreservation to save coconut germplasm in areas infected by lethal yellowing. African Journal of Biotechnology , 13(16), 1702-1706.
Chicago Oulo Alla-N&#;Nan., Sery Gon&edel&e Bi, Kouakou Ti&ecoura, Jean-Louis Konan Konan and  Bernard Malaurie. "Use of plumules cryopreservation to save coconut germplasm in areas infected by lethal yellowing." African Journal of Biotechnology 13, no. 16 (2014): 1702-1706.
MLA Oulo Alla-N#;Nan., et al. "Use of plumules cryopreservation to save coconut germplasm in areas infected by lethal yellowing." African Journal of Biotechnology 13.16 (2014): 1702-1706.
   
DOI 10.5897/AJB2014.13670
URL http://www.academicjournals.org/journal/AJB/article-abstract/0FCAD9243980

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