African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12011

Full Length Research Paper

Use of plumules cryopreservation to save coconut germplasm in areas infected by lethal yellowing

Oulo Alla-N'Nan*
  • Oulo Alla-N'Nan*
  • Université Félix Houphouet Boigny, 22 BP 582 Abidjan 22, UFR Biosciences, laboratoire de génétique, Côte d'Ivoire; IRD, UMR DIADE, 34394 Montpellier Cedex 5, France.
  • Google Scholar
Sery Gonédelé Bi
  • Sery Gonédelé Bi
  • Université Félix Houphouet Boigny, 22 BP 582 Abidjan 22, UFR Biosciences, laboratoire de génétique, Côte d'Ivoire.
  • Google Scholar
Kouakou Tiécoura
  • Kouakou Tiécoura
  • Université Félix Houphouet Boigny, 22 BP 582 Abidjan 22, UFR Biosciences, laboratoire de génétique, Côte d'Ivoire.
  • Google Scholar
Jean-Louis Konan Konan
  • Jean-Louis Konan Konan
  • Centre National de Recherche Agronomique (CNRA), Programme cocotier, Station de recherche Marc Delorme, 13 BP 989 Abidjan 13, Côte d’Ivoire.
  • Google Scholar
Bernard Malaurie
  • Bernard Malaurie
  • IRD, UMR DIADE, 34394 Montpellier Cedex 5, France.
  • Google Scholar


  •  Received: 28 January 2014
  •  Accepted: 14 April 2014
  •  Published: 16 April 2014

Abstract

Plumules excised from zygotic embryos through the largest representative diversity of four of the five different areas of coconut cash and food crops were used in a cryopreservation process using encapsulation-dehydration technique. Five accessions of coconut trees were used [Panama Tall (PNT/GPA), Brazilian Green Tall (BGD/NVB), Cameroon Red Dwarf (CRD/NRC), Vanuatu Tall (VTT/VNT/GVT), and Tagnanan Tall (TAGT/GTN)] in addition to the accession model [Malayan Yellow Dwarf (MYD)] from which an optimal protocol was obtained. A great variability of response was observed depending on accessions with survival and growth recovery rates varying from 6 to 66% and 0 to 24% after 2 and 7 months of culture, respectively.

 

Key words: Coconut, accessions, germplasm, plumules, cryopreservation, encapsulation-dehydration.