African Journal of Agricultural Research
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Article Number - EF3A32E37994


Vol.8(36), pp. 4580-4587 , September 2013
DOI: 10.5897/AJAR12.1590
ISSN: 1991-637X



Full Length Research Paper

Investigation of respiratory viruses in camel slaughtered at Addis Ababa Akaki Abattoir, Ethiopia


Gelagay Ayelet1*, Wosenne Negash1, Tesfaye Sisay2, Shiferaw Jenberieand Esayas Gelaye1




1National Veterinary Institute, P. O. Box: 19, Debre Zeit, Ethiopia.

2School of Veterinary Medicine, Addis Ababa University, P. O. Box: 34, Debre Zeit, Ethiopia.


Email: gelagayayelet@yahoo.com.






 Accepted: 24 July 2013  Published: 19 September 2013

Copyright © 2013 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0


Camel is an important domestic animal uniquely adapted to the hot and arid environment, but its contribution to Ethiopian pastoralists is disproportionate to its resource potential due to the presence of various infectious and parasitic diseases in the area. Therefore this study was conducted with an attempt to isolate and characterize respiratory viruses from infected lungs. Accordingly, camel lung tissues were collected from Akaki abattoir and transported to National Veterinary Institute virology laboratory and stored temporarily at -85°C. The samples were processed and inoculated on confluent VERO cells, incubated at 37°C and examined for the development of cytopathogenic effect (CPE) for 4 to 15 days. From thirty five examined samples twenty seven were CPE positive.  Ten supernatant samples exhibiting CPE were taken and tested for presence of both DNA and RNA viruses, using universal degenerate oligonucleotide primed– polymerase chain reaction (DOP-PCR) and conventional polymerase chain reaction (PCR) techniques. Five out of six samples tested by DOP-PCR were positive for presence of RNA virus, while only one sample was positive for DNA virus. Nine of ten samples were positive for, Respiratory syncytial virus (RSV) and two of six were positive forAdenovirus, but all tested samples were negative for Peste Des Petits Ruminants Virus (PPRV) andParainfluenza viruses 1-3.

 

Key words: Abattoir, camel, pneumonia, respiratory viruses, polymerase chain reaction.


APA (2013). Investigation of respiratory viruses in camel slaughtered at Addis Ababa Akaki Abattoir, Ethiopia. African Journal of Agricultural Research, 8(36), 4580-4587.
Chicago Gelagay Ayelet, Wosenne Negash, Tesfaye Sisay, Shiferaw Jenberie and Esayas Gelaye. "Investigation of respiratory viruses in camel slaughtered at Addis Ababa Akaki Abattoir, Ethiopia." African Journal of Agricultural Research 8, no. 36 (2013): 4580-4587.
MLA Gelagay Ayelet, et al. "Investigation of respiratory viruses in camel slaughtered at Addis Ababa Akaki Abattoir, Ethiopia." African Journal of Agricultural Research 8.36 (2013): 4580-4587.
   
DOI 10.5897/AJAR12.1590
URL http://www.academicjournals.org/journal/AJAR/article-abstract/EF3A32E37994

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