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Full Length Research |
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Isolation,
identification and determination of methyl caffeate, ethyl
caffeate and other phenolic compounds from Polygonum
amplexicaule var. sinense
Meixian Xiang1,2,3, Hanwen Su4,
Jinyue Hu4 and Yunjun Yan1,2,3*
1College
of Life Science and Technology, Huazhong University of Science
and Technology, Wuhan 430074, Hubei Province, P. R., China.
2Key
Laboratory of Molecular Biophysics, Ministry of Education, Wuhan
430074, Hubei Province, P. R., China.
3College
of Pharmacy, South-Central University for Nationalities, Wuhan
430074, Hubei Province, P. R. China.
4Renmin
Hospital Attached to Wuhan University, Wuhan 430061, Hubei
Province, P. R., China.
*Corresponding
author.
E-mail:
yanyunjun@mail.hust.edu.cn. Tel: +86 027 87792213
Accepted 26 November, 2010 |
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Abstract |
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Polygonum amplexicaule
D. Don (Polygonaceae) (Chinese name: Xuesanqi) is a
widely distributed medicinal plant in China. It has been used in
folk medicine to treat many common and frequently-occurring
diseases, such as
cardiovascular and cerebrovascular diseases, fractures, pain,
etc. Thus, this plant has been of interest to researchers.
However, the chemical constituents of Xuesanqi have not
been investigated systematically. This study isolated and
identified some effective compounds in this plant. The structure
of important active chemicals of Xuesanqi from different
regions was determined for the first time. Twelve phenolic
compounds were isolated from this plant. Vanillin, isovanillic
acid, p-hydroxyphenethyl alcohol, dihydro-kaempferol, and
5,7-dihydroxychromone were determined for the first time from
Polygonum. As markers for quality standard of this herb, the
content of methyl caffeate and ethyl caffeate was determined by
HPLC. It was found that Xuesanqi from Wufeng, Lichuan,
Badong Counties in Hubei Province and Jiujiang City of Jiangxi
Province had higher content of both chemicals. The results
suggest that the herb might be a potential source of phenolic
compounds of an agent for improving blood circulation, treating
various fractures, muscle and tissue swelling, and pain.
Key words: Chemical constituents, Xuesanqi, analysis,
high-performance liquid chromatography (HPLC).
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