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  J. Med. Plants Res.

 

  Vol. 4 No. 3
 

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  articles by:
 

  Meftahizade H

  Naseri A

 


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Journal of Medicinal Plants Research Vol. 4 (3), pp. 240–244, 4 February 2010

ISSN 1996-0875 © 2010 Academic Journals

   

 

Full Length Research Paper

 

  
 

Improved in vitro culture and micropropagation of different Melissa officinalis L. genotypes

 

H. Meftahizade1*, H. Moradkhani2, B. Naseri3, M. Lofti4 and A. Naseri1

 

1ACECR Medicinal Plants Research Center, Ilam Iran.

2Student of Agricultural Biotechnology, Armenia.

3Faculty of Islamic Azad University, Ilam, Iran.

4Department of Horticultural, Aboureihan Campus, University of Tehran, Iran.

 

*Corresponding author. E-mail: Hmeftahi@yahoo.com, Heidarmeftahi732@gmail.com.

 

Accepted 16 December, 2009
 
     
 

Abstract

  
     
 

Melissa officinalis L. is widely cultivated in the world and Iran as well, due to its useful application in medicine. In order to establish a stable and high frequency of regeneration system with 4 landrace collected from different climate in Iran, major parameters such as regeneration rate, rooting percentage, shooting and proliferation rate were investigated. Statistical analysis of results showed that BAP in combination with NAA had the highest regeneration in shoot tips explants. NAA in combination with IAA and Kinetin had the best response to callus induction. Also 1 mgl/l NAA had the higher response to rooting than other’s used auxins. Explants derived from Hamedan landrace had the highest potential for regeneration. This is the first report on high frequency in vitro regeneration in four diverse landraces of M. officinalis selected from various climates regions in Iran.

 

Key words: Melissa officinalis, regeneration, micropropagation, Iran. 

  

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