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  J. Med. Plants Res.

 

  Vol. 4 No. 4
 

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  articles by:
 

  Liu G

  Hu X

 


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Journal of Medicinal Plants Research, Vol. 4(4) pp. 298–303, 18 February, 2010

ISSN 1996-0875 © 2010 Academic Journals  
   

 

Full Length Research Paper

 

  
 

Simultaneous determination of 6 active components in traditional Chinese medicine “KANGXIN” tablets by RP-HPLC–DAD

 

Gang Liu1, Hui Wang1*, Yi Luo1, Benhong Zhou1 and Xianming Hu2

 

1Department of Pharmacy, Renmin Hospital, Wuhan University,

Wuhan 430060, PR China.

2College of Pharmacy, Wuhan University, Wuhan 430072, PR China.

 

*Corresponding author. E-mail: mailto:liugang.wh@gmail.com, liugang.wh@gmail.com.

 

Accepted 14 December, 2009
 
     
 

  Abstract

  
     
 

A HPLC method was developed for simultaneous quantitative determination of the 6 compounds namely aloin, puerarin, genistein, daidzin, genistin and daidzein in “KANGXIN” tablets. The separation was achieved on a C18 column, a diode array detector at 260 nm and a mobile phase composed of acetonitrile and water under the gradient elution. Good linear relationships were obtained. The average recoveries were in the range of 95.83–102.57%. The validated method can be utilized as a suitable method for the quality control of “KANGXIN” tablets.

 

Key words: Column liquid chromatography, active components, “KANGXIN” tablet, quality control.

  

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