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  J. Med. Plants Res.

 

  Vol. 4 No. 4
 

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  Gilani SA

  Watanabe KN

 


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Journal of Medicinal Plants Research Vol. 4(4), pp. 309315, 18 February 2010

ISSN 1996-0875 © 2010 Academic Journals    

   

 

Full Length Research Paper

 

 
 

Chemotypic variations and phytotoxic studies of essential oils of endemic medicinal plant, Seriphidium kurramense, from Pakistan

 

Syed Abdullah Gilani1, 2*, Yoshiharu Fujii3, Mami Sugano3 and Kazuo N. Watanabe2

 

1Department of Botany, Kohat University of Science and Technology (KUST), Kohat, Pakistan.

2Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan.

3Biodiversity Division, National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan.

 

*Corresponding author. Email: gilani_abdullah@yahoo.com

Tel: +81-29-853-7729.

 

Accepted 13 January, 2010

 
     
 

 Abstract

 
     
 

Seriphidium kurramense is an endemic and economic medicinal plant growing in a small narrow valley of tribal region in Pakistan, Upper Kurram Agency, at Pakistan-Afghanistan border. GC-MS analysis of essential oils of S. kurramense identified 28 chemical constituents from 16 populations with two distinct chemotypes, A and B. Both chemotypes represented similar morphological features. Major chemical constituents of chemotype A comprising 12 populations, consisted of α-thujone (26.0 - 73.4 %), β-thujone (3.14 - 49.3 %), 1,8-cineole (10.2 - 22.3 %) and camphor (0 - 26.3 %), while the major chemical constituents of chemotype B from four populations were p-menthan-1,8-diol (23.1 - 35.2 %), 1,8-cineole (10.2 - 23.8 %) and an unknown chemical compound (12.5 - 25.9 %). Significant differences in toxicity levels were observed in all the 16 populations of S. kurramense. Chemotype B showed highest root and Hypocotyle inhibition of lettuce seeds as compared to Chemotype A.

 

Key Words: Seriphidium kurramense, endemic, GC-MS, chemotypic variation, Kurram Agency, Pakistan, phytotoxic.

 

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