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J. Cell Anim. Biol.


Vol.3 No. 11



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Fasuyi AO

Kehinde OA


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Journal of Cell and Animal Biology Vol. 3 (11), pp. 188-195  November, 2009 ISSN 1996-0867  ©2009 Academic Journals  

 

Full Length Research Paper

 

 

Effect of cellulase-glucanase-xylanase combination on the nutritive value of Telfairia occidentalis leaf meal in broiler diets

 

A. O. Fasuyi* and O. A. Kehinde

 

Department of Animal Production and Health Sciences, University of Ado-Ekiti, Ekiti State, Nigeria.

 

*Corresponding author. E-mail: dejifasuyi@yahoo.com.

 

Accepted 10 September, 2009

 

   Abstract

 

Telfairia occidentalis leaves were purchased fresh from local farmers, sun dried and milled into T. occidentalis leaf meal (TOLM). The proximate analyses revealed that TOLM contained 35.14 ± 0.44% crude protein; 9.61 ± 0.01% fat; 12.68 ±  0.02% crude fibre; 10.87 ± 0.02% ash; 9.04 ± 0.13% dry matter and 3.25 ± 0.01 kcal/ g gross energy. Eight broiler starter diets were formulated to be isocaloric and isonitrogenous. Diet 1 was duplicated such that the enzyme combination of cellulase, glucanase and xylanase (Roxazyme G2) was incorporated into one while Roxazyme G2 was lacking in the other (control diets 1a & 1b, with and without enzyme supplementation). Diets 2 and 3 were duplicate diets at 10% TOLM inclusion levels with the only difference being the Roxazyme G2 supplementation in diet 3. Diets 4 and 5 were also duplicates at 20% TOLM inclusion level with Roxazyme G2 supplementation in diet 5. Diets 6 and 7 at 30% TOLM inclusion were also in duplicate with diet 7 having Roxazyme G2 supplementation. The weight gain (WG) value for chickens on diet 1b with enzyme supplementation was highest at 19.51 ± 2.10g/chick although similar (P > 0.05) to WG values for birds on diets 1a (without enzyme supplementation), 2 and 3 while diet 7 with 30% TOLM inclusion with Roxazyme G2 supplementation had the lowest WG of 11.09 ± 0.39g/chick. Feed consumption (FC) was similar and higher in birds on diets 1a & b, 2 and 3. FC was also similar and lower in birds on diets 4, 5, 6 and 7. Feed conversion ratios did not differ significantly among the experimental diets except Diet 7. The operative protein efficiency ratio (PER) was higher and similar (P > 0.05) for birds on diets 1a & b, 2 and 3 on one hand and lower and similar (P>0.05) for birds on diets 4, 5, 6 and 7 on the other hand. The nitrogen retention (NR) value was highest for diet 1b (with enzyme supplementation) at 72.37 ± 0.10 but similar (P > 0.05) to birds on diets 1a, 4 and 5. There were no significant differences (P > 0.05) among means of the heamatological parameters tested. The mortality during the experimental period was insignificant and less than 1% of the total number of birds used.

 

Key words: Enzyme supplementation, leaf meal, Roxazyme G2, chicks, poultry feed

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