African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007

AJMR Articles in press

Article in Press

Differential niche occupation and the biotechnological potential of Methylobacterium spp. associated with sugarcane plants

This work highlighted a putative link between the physiological activity and genetic diversity of Methylobacterium spp. and the association with sugarcane roots and rhizoplane. In total, 40 isolates previously described as pink-pigmented facultative methylotrophic bacteria (PPFMs), were evaluated for their ability to fix nitrogen and solubilize inorganic phosphate, amylase and pectinase activity. This in vitro potential...

Author(s):Pedro Avelino Maia de Andrade, Armando Cavalcante Franco Dias, Simone Raposo Cotta, Diogo Paes da Costa, João Tiago Correia Oliveira, Lucianne Ferreira Paes de Oliveira, Fernando José Freire, Fernando Dini Andreote, Julia Kuklinsky-Sobral

Article in Press

Paediatric tuberculosis in a low burden setting of Saudi Arabia: Drug and multidrug resistance patterns

This study investigates and discusses the infection of young children with Mycobacterium tuberculosis and drug-resistant M. tuberculosis in a mass gathering area, Al-Madinah Al-Munawwarah. All children, 15 years old and younger, who were referred to the central tuberculosis laboratory in Al-Madinah between January 2012 and December 2014 were included in this study. Among a total of 622 registered new cases, 68 (10.9 %)...

Author(s):Mogahid M. Elhassan, Miskelyemen A. Elmekki, Hani A. Ozbak, Hassan A. Hemeg, Khalid A. Turkistani, Shamsoon K Kafi and Ahmed A. Abdul-Aziz

Article in Press

Biotechnological and industrial applications of enzymes produced by extremophilic bacteria. A mini review

Extremophilic bacteria are important groups of extremophilic organisms that have been studied during the two decades. The demand for enzymes from extremophilic bacteria has increased considerably due to its potential industrial application and great diversity and can work under extreme conditions. Due to their activity and stability under extreme conditions, these enzymes offer new alternatives for current biotechnological...

Author(s):Kelly Dumorné

Article in Press

Prevalence, cytotoxicity and antibiotic susceptibility of Campylobacter species recovered from retail chicken meat in Mansoura, Egypt

This study was performed to determine the prevalence of Campylobacter species in retail chicken meat and chicken by-product, determine their in vitro cytotoxicity, as well as, examine their susceptibility to different antimicrobials. A total of 300 raw chicken meat samples were collected from different retail chicken meat outlets located at Mansoura city, Egypt classified into 120 thighs, 120 breasts, and 60 livers. All...

Author(s):A. A. Hafez, G. Younis, M. M. El-Shorbagy and A. Awad

Article in Press

Serological detection of hepatitis B virus infection in patients under hemodialysis in White Nile State of Sudan

Hemodialysis (HD) predisposes patients to blood born viruses infections especially HBV. This work was conducted to estimate the frequency of Hepatitis B virus infection among hemodialysis patients. During the period from August to November 2017, a total of 100 blood samples (68 males ,and 32 females) were collected. Each sample was serologically tested for HBsAg using immunochromatographic tests according to manufactures...

Author(s):Rayan Nader Ali, Tasneem Rhamt Allah, Babiker Saad Almugadam

Article in Press

Detection of alpha toxin and enterotoxins of Clostridium perfringens isolated from minced meat by Real time PCR

Clostridium perfringens is one of the most widespread pathogen producing toxins related to variable pathogenic conditions, particularly food poisoning in humans. Thus, this study described the prevalence, enumeration, toxigenic types and antibiotic susceptibility of C. perfringens strains isolated from minced meat in Egypt as well as the validation of a real-time polymerase chain reaction (PCR) test for the identification...

Author(s):Gamal A. Younis, Mona M. Radwan, Rasha M.Elkenany and Marwa H. Elderieny

Article in Press

Phylogenetic diversity of prokaryotes on the Snow-cover of Lewis Glacier in Mount Kenya

The seasonal snowpack of the temperate glaciers are sources of diverse microbial inoculi. However, the microbial ecology of the tropical glacial surfaces is endangered, hence poses an extinction threat to some populations of some microbes due to rapid loss of the glacier mass. The aim of this study was to isolate and phylogenetically characterise the prokaryotes from the seasonal snow of Lewis glacier in Mt. Kenya. Snow...

Author(s):Josiah O. Kuja, Huxley M. Makonde, Hamadi I. Boga, Anne T.W. Muigai, Jun Uetake

Article in Press

FOODS, FISH AND SALMONELLOSIS

Foodborne diseases are those caused by the consumption of water and food contaminated by different causal agents such as: viruses, bacteria, parasites, toxins, among others, being considered an important public health problem global due to its incidence and mortality and for several years for the isolation of microorganisms that cause these diseases resistant to antimicrobials. Salmonella spp ., is considered a food...

Author(s):Alejandro De Jesús Cortés-Sánchez and Luis Daniel Espinosa-Chaurand

Article in Press

Microbiological quality of fruit juices sold in cafes and restaurants of Shewarobit town, Amhara, Ethiopia

Fresh and unpasteurized fruit juice is common in restaurants, cafeteria, hotels and juice house of Ethiopian cities. Most fruit juices contain sufficient nutrients that could support microbial growth. The current investigation was carried out to investigate the microbiological quality and processing conditions of fruit juice vended in Shewarobit town. Purposive sampling technique was employed to collect sixteen fruit juice...

Author(s):Bulti Kumera Fufa and Melkam Dessalegn Liben

Article in Press

RESPONSE OF MICROBIAL COMMUNITIES TO OIL SPILL IN THE GULF OF MEXICO; A REVIEW

Crude oil has become a part of the marine ecosystem through natural seeps and oil spills. Microbial communities have adopted various response mechanisms to adjust to oil spills that contaminate the marine environment and help restore the ecosystem to its original state. These response mechanisms ranges from change in indigenous microbial community composition, change in microbial diversity to gene diversity and...

Author(s):Linda Adzigbli, Hernado P. Bacosa, Yuewen Deng

Article in Press

Fungal endophytes isolated from the leaves of a medicinal plant Ocimum sanctum Linn and the evaluation of their antimicrobial activities

The endophytic fungi isolated from leaves of Ocimum sanctum Linn. leaves of different ages were examined for antimicrobial activity. The agar plug diffusion assay was used for primary screening. A total of 148 fungal endophytes were successfully isolated and cultured but only 134 of them (90.5%) exhibited inhibitory activity towards at least one test microorganisms. Moreover, the colonization rate indicated that the old...

Author(s):Taufiq, M. M. J. and Darah, I.

Article in Press

Assessment of microbiota in root canals with pulp necrosis by means of Gram test

The aim of this study was to evaluate the type of microbiota present in root canals with pulp necrosis, with and without periapical lesion. Nineteen patients were selected for the study and 30 root canals were analysed in unirradicular and/or multi-radicular permanent teeth, asymptomatic, with pulp necrosis, with or without periapical lesion, and no communication between root canal and oral cavity. Absorbent paper cones...

Author(s):Amaro de Mendonça Cavalcante, Natalia Maria Luccas Tenório Soares, Isabel Cristina Secandes Santos, Eulália Camelo Pessoa de Azevedo Ximenes, Marcos Aurélio Bomfim da Silva and Karlos Antonio Lisboa Ribeiro Junior

Article in Press

Bacteriological quality of drinking water at main sources, reservoirs and consumers’ tap water in Arba Minch town, Southern Ethiopia

The most common and wide spread health risk associated with drinking water is microbial contamination. The aim of this study was to assess microbial contamination of drinking water, starting from source to distribution systems. Water samples of the Arba Minch town of Southern Ethiopia were collected randomly from the main source before chlorination, reservoir after chlorination and from different points of distribution...

Author(s):Gemechu Ameya, Olifan Zewdie, Abdulhakim Mussema, Adugna Amante, Birhanie Asmera

Article in Press

Marine enzymes and their industrial and biotechnological applications. A mini review

Marine microorganisms are considered as a great source of biodiversity. The demand of marine enzymes are extensively and increasingly used in research and in various industries due to their diversity to survive under extreme conditions such as saline conditions, pH, temperature, light, atmospheric pressure, and the availability of nutrients. Despite, their huge biodiversity and benefits that present marine enzymes their...

Author(s):Kelly Dumorné and Marcia Astorga Élo

Article in Press

Isolation of bacterial diversity present in medical waste and health care settings in hospitals in Kenya

Nosocomial infections have impacted great burden in healthcare system and has led to deteriorating health condition and deaths. Current study characterizes medically important bacterial diversity, isolated from staff hands, hospital surfaces and wastes in healthcare settings in Kenya during a one year period. Descriptive cross sectional hospital based study design and simple random sampling method was used to collect 246...

Author(s):Susan Muthoni Maina, Andrew K Nyerere and Caroline Wangari Ngugi

Article in Press

Antimicrobial resistance patterns of Enterobacteriaceae recovered from wastewater, sludge and dumpsite environments in Kakamega town, Kenya

Enteric bacterial resistance to antibiotics and the emergence of resistant pathogens in the environment is a global threat to public health. In Kenya, sewage treatment plants are not designed to eliminate enteric microbes, whereas domestic, medical and other hazardous wastes are all discarded in common solid-waste dump sites. Arising from these practices, waste treatment sites in developing countries may be important...

Author(s):Clarice Malaho, Sifuna Anthony Wawire and William A. Shivoga

Article in Press

Effect of indigenous and effective microorganism fertilizers on soil microorganisms and yield of Irish potato in Bambili, Cameroon

Irish potato (Solanum tuberosum) is one of the world’s most consumed staple worldwide and an important crop in terms of food security in the face of population growth and increased hunger rates. Potato yields in Cameroon have often been low as a result of decrease in soil fertility. Soil fertility has often been regarded as the chemical and physical properties of soil, with the microbial aspect often being ignored. An...

Author(s):Taffouo Victor Desiré, Muyang Rosaline Fosah, Mbouobda Hermann Desiré and Fotso

Article in Press

Screening of bioactive compound, antimicrobial activity producing halophilic isolates from the saltpans of Thoothukudi district

Extreme environments harbor a number of microbes producing novel bioactive compounds. The aim of our study is to isolate and identify bioactive compound producing halophiles. Marine soil sediments were collected from the solar saltpans of Thoothukudi District, Tamil Nadu, India. Based on colony morphology, two species were isolated and identification was done by using morphological and biochemical tests. The extracts of...

Author(s):Deepalaxmi R. K. and Gayathri C.

Article in Press

Evaluation of gene xpert assay and microscopic examination for detection of Mycobacterium tuberculosis in sputum at Port Harcourt, Nigeria

The re-emergence and increase in the prevalence of tuberculosis cases and multidrug resistant strains is of public health concern. The conventional Acid Fast Bacilli detection tool is weak in the current disease trend especially in children, patients with human immunodeficiency virus (HIV) and low bacterial load. The aim of this research was to assess effectiveness of the Gene Xpert MTB/RIF assay and Microscopy in...

Author(s):Confidence Kinikanwo Wachukwu, Stella Paulinus Okwelle, Easter Godwin Nwokah and Ollor Amba Ollor

Article in Press

Ameliorative effect of imipenem on pulmonary damage caused by extended spectrum β-lactamase (ESBL) producing bacteria isolates from non- human source

Acute lung infection induced by Extended Spectrum β-Lactamases (ESBL) producing isolates was determined by measuring inflammatory mediators; malondialdehyde (MDA), myeloperoxidase (MPO) and nitric oxide (NO). The mice were randomly divided into three groups of 20 animals each. All mice were given 104 c.f.u. ml-1 of the test organism intranasally in a volume of 50 µl while holding the mouse in an upright position without...

Author(s):Afunwa, R. A., Iroha, R. I., Ugwu, M., Afunwa, E. C., Unachuchwu, M. N. and Esimone, C. O.

Article in Press

Assessment of antimicrobial resistance patterns in Escherichia coli isolated from clinical samples in Madinah, Saudi Arabia

Escherichia coli is a Gram-negative bacteria that causes various diseases, including pneumonia, urinary tract infections, and diarrhoea. The present work is an effort to study antimicrobial resistance pattern in this bacteria. Clinical samples (6840) were collected from King Fahd Hospital in Madinah, Saudi Arabia and screened for E. coli strains. Of all positive samples obtained from different clinical sources, about 3%...

Author(s):Walaa M. Saeed, Samah Ghanem, Hatem M. El Shafey and Nikhat Manzoor

Article in Press

Incidence of bacterial infection in chronic hepatitis C virus (HCV) patients with cirrhosis and association between toll-like receptor 4 D299G gene polymorphism and Gram- negative bacterial infections in the patients

This study aimed to investigate the incidence of bacterial infection in chronic hepatitis C virus (HCV) patients with cirrhosis and association between toll-like receptor 4 D299G gene polymorphism and Gram-negative bacterial infections in the patients. 100 HCV cirrhotic patients with ascites and 20 age- and sex-matched healthy subjects as control were included. Conventional culture methods were used to identify the...

Author(s):Hanan El-Bassat, Lobna Abo Ali, Atef Taha and Rasha Alm El-Din

Article in Press

Assessment of the prevalence of extended-spectrum β-lactamase producing Gram-negative bacilli at the Charles De Gaulle Paediatric University Hospital (CDG-PUH), Ouagadougou, Burkina Faso

Bacterial resistance to antibiotics is a serious concern in developing countries due to precarious hygiene conditions, inappropriate prescription as well as self-medication and free sale of antibiotics. This study was aimed to assess the prevalence of strains producing extended-spectrum beta- lactamase in the Gram negative bacteria isolated in the laboratory. The study was conducted in the Charles De Gaulle Paediatric...

Author(s):Mahamoudou Sanou, Absétou Ky/Ba, Pricille Coulibali, Marius Nagalo, Abdoul Salam Ouédraogo, Mamadou Tamboura, Dinanibé Kambiré, Cyrille Bisseye, Fidèle Bakiono, Jacques Simporé and Ramata Ouédraogo

Article in Press

Management of bacterial wilt disease of potato in coastal plains of Odisha

Bacterial wilt and brown rot caused by Ralstonia solanacearum is one of the most dreaded diseases causing 30 to 70% yield loss in India. The disease is both tuber and soil borne. A field trial was conducted following split plot design in the year 2008 to 09 and 2009 to 10 under All India Co-ordinated Potato Research Project in Central Farm, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India with...

Author(s):Biswal, G. and Dhal, N.K.

Article in Press

Isolation, identification and evaluation of mosquito entomopathogenic Bacillus species and related genera from randomly selected sites in Kenya

This study was carried out to identify specific mosquitocidal Bacillus species and related genera for future development of biopesticides in local mosquito control program in Kenya. Bacterial isolation was conducted from 100 soil samples through pasteurization method and preliminary identification conducted through phenotypical analysis. Toxicity analysis was performed through bioassays and lethal concentrations (LC) were...

Author(s):Samuel Karungu, Doudou Huang, Evans Atoni, Cecilia Waruhiu, Bernard Agwanda, Xiaomin Hu and Zhiming Yuan

Article in Press

Influence of subminimal inhibitory concentrations of some bioactive compounds on the biofilm formation ability and virulence factors of multiple drug resistant Acinetobacter baumannii clinical isolates

The objective of this study was to assess the impact of subminimum inhibitory concentrations (sub-MICs) of chosen antimicrobial agents (Amikacin, imipenem, benzalkonium chloride, and chlorhexidine) and natural product (garlic) on biofilm formation ability, bacterial adherence and invasion. Susceptibility profiles of 50 non-repetitive Acinetobacter baumannii clinical isolates to eight antibiotics were investigated. MIC of...

Author(s):Mona H. Mahmoud, Magdy A. Amin, Rania I Shebl, Aly F. Mohamed and Mai M. Zafer

Article in Press

Physiochemical factors affecting in vitro growth of Pasteurella multocida

Pasteurella multocida causes fowl cholera (FC), a contagious bacterial disease of domestic and wild avian species. It is a Gram negative coccobacilli causing acute highly fatal septicemia with high morbidity and mortality rate. Control of fowl cholera is primarily performed by good management practice and vaccination in areas where disease is endemic. For a quality vaccine, high density growth of P. multocida along with...

Author(s):Mehmood M. D., Zia S., Javed F., Gul M., Ashraf M. and Anwar H.

Article in Press

Antimicrobial activity of crude extracts of endophytic fungi from Oryctanthus alveolatus (Kunth) Kuijt (Mistletoe)

The use of antibiotics has unleashed high bacterial resistance. This outcome triggered the urgent need for effective new antibacterial agents to treat infectious diseases. A promising source for the production of antibiotics and several other bioactive substances are endophytic fungi. These microorganisms inhabit in and bring benefits to living plant tissues. Thus, the aim of this paper was to know the endophytic fungi...

Author(s):Sanay Feitosa Lima Ribeiro, Armando da Costa Garcia, Hathyla Eduarda Dias dos Santos, Quimi Vidaurre Montoya, Andre Rodrigues, João Marcos de Oliveira and Camila Martins de Oliveira

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