African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007

AJMR Articles in press

Article in Press

Fermentation properties and monacolin K formation by Monascus spp in brewing rice wine

Objectives To improve the content of monacolin K in rice wine, fermentation properties and monacolin K formation by red yeast rice collected from Southern China were studied while brewing new functional rice wine . Results Red yeast rice Ⅰ and red yeast rice Ⅲ have fast fermentation dynamics. The content of monacolin K is higher in red yeast rice Ⅰ that can be up to 0.86 ug / ml; the rice wine brewed by the red yeast...

Author(s):Zhipeng Wang •Zhaoyang Qin•Cuicui Wang • Guomin Wang • Hairu Jin

Article in Press

Candida albicans interdigital foot infection: A case report highlighting the importance of antifungal susceptibility testing

Candida species are opportunistic fungal pathogens which are often isolated from immunocompromised individuals. C. albicans is the most frequently isolated species in both superficial and invasive candidiasis. Non-albicans species are equally striving in their pathogenic ability, but it is noticed that C. albicans continues to exert its relevance as a leading cause of candidiasis as confirmed according to this case report...

Author(s):Yahaya Hassan, Sharif Alhassan Abdullahi and Leslie Thian Lung Than

Article in Press

Screening of human diarrhoeal samples in Mymensingh city of Bangladesh for the isolation, identification and antimicrobial resistance profiles of Campylobacter spp.

Campylobacter spp. (Campylobacter jejuni and Campylobacter coli) are one of the major cause of food-borne bacterial diarrhoea in human worldwide. This study was conducted for the isolation, identification and antimicrobial resistance profiling of Campylobacter spp. from diarrhoeal samples of human collected from SK (Surya Kanta) hospital, Mymensingh Medical College, Mymensingh during the period of August 2016 to October...

Author(s):Sudarsan Karmaker, S. M. Lutful Kabir, A.K.M. Ziaul Haque, Mohammad Ferdousur Rahman Khan and Yousuf Ali Sarker

Article in Press

Molecular detection and characterization of Escherichia coli, Salmonella spp. and Campylobacter spp. isolated from broiler meat in Jamalpur, Tangail, Netrokona and Kishoreganj districts of Bangladesh

The study was conducted to isolate, identify and characterize bacterial samples from broiler meat collected from 20 different upazilla markets of Jamalpur, Tangail, Netrokona and Kishoreganj districts of Bangladesh. A total of 20 samples were subjected to bacteriological isolation and identification, and the isolated bacteria were subjected to antimicrobial susceptibility testing using disc diffusion method. Among the...

Author(s):Md. Kamrul Islam, S. M. Lutful Kabir, A.K.M. Ziaul Haque, Yousuf Ali Sarker and Mahmudul Hasan Sikder

Article in Press

Antibacterial activity of Ocimum gratissium (scent leaf) on some pathogenic gastrointestinal bacteria

The emergence of antibiotic resistance as well as the recent undesirable side effect of some of the commercially available antibiotics has led to the screening of plant extract in order to discover new drug that could serve as alternative therapy for the treatment of various infections and diseases. Fresh leaf of Ocimum gratissimum (scent leaf) sample was collected, air-dried at room temperature and blended to powder using...

Author(s):AGHOLOR KIN; LUCY M. YAKI; IDRIS ABUBAKAR; LUCY F. OLUSOLA

Article in Press

Optimisation of biological wastewater treatment for yeast processing effluent using cultured bacteria: Application of Response Surface Methodology

In the present study, the interactive effects of temperature and cultured bacteria on the performance of a biological treatment system of wastewater from a local yeast producing plant were investigated. The main objective of this study was to optimize the operating parameters that reduce organic load and colour. Biological treatment was conducted using a Central Composite Design (CCD) and optimised using Response Surface...

Author(s):Manhokwe S, Shoko S and Zvidzai C

Article in Press

Isolation and characterization of starch degrading rhizobacteria from soil of Jimma University Main Campus, Ethiopia

Starch degrading bacteria are important for different industries such as food, fermentation, textile, and paper. The aim of this study is to isolate and characterize bacteria able to degrade starch from the rhizospheres of various plants at four sites located in Jimma University main campus. Collected soil samples were labeled as kobo (AJUMC), Avocado (BJUMC), Banana (CJUMC), and Cana indicia (DJUMC) respectively. Soil...

Author(s):Gudeta Dida

Article in Press

Antibacterial, muscle relaxant, and hypnotic effects of seeds of Peganum harmala on mice

Peganum harmala seed extract has been frequently reported to posses antibacterial potential through invivo studies. Peganum harmala L. (Zygophyllaceae) is one of the most famous medicinal plants used in traditional medicine of Iraq. The harmaline, harmalol and harmine exerted many pharmacodynemic effect on the central nervous system, ranged between stimulation and depression depending on the dose. Peganum harmala indicate...

Author(s):Thamer Mutlag Jasim

Article in Press

Characterization of microorganisms isolated from ruminant fermented water hyacinth (Eichhornia crassipes) feed

The aim of this study was to determine the number of bacteria and fungi in the fermentation process and characterization of these microorganisms. There were several steps in this research including production of fermented feed, isolation of indigenous microorganisms, purification, characterization of microorganisms, species identification and test of cellulolytic activity. Results showed that eight bacteria and four fungi...

Author(s):Isnawati, Guntur Trimulyono

Article in Press

Chemical compositions, in vitro antibacterial behavior and antioxidant effect of volatile oil and phenolic extracts from wild Juniperus phoenicea L. growing in Southwest of Algeria

This work examines the chemical composition, antimicrobial and antioxidant activities of four phenolic extracts and essential oil of Juniperus phoenicea L. (cuprecaceae) aerial parts growing spontaneously in el Bayedh region of southwestern Algeria. The hydro-distillated essential oil from this plant was characterized by gas chromatography-mass spectrometry (GC-MS). This oil is mainly composed of monoterpene and...

Author(s):Ghouti Dalila, Lazouni Hamadi Abderrahmane, Moussaoui Abdallah and Chabane Sari Daoudi

Article in Press

Antimicrobial susceptibility pattern of Gram negative bacteria isolated from intensive care units in Al-Ahsa, Kingdom of Saudi Arabia

Antimicrobial resistance by bacteria isolates continues to receive attention globally. This investigation looks into the antibiotic susceptibility pattern of Gram negative bacteria isolated from intensive care unit patients in Al-Ahsa, KSA. Bacteria samples were classified based on the CDC criteria for the definition of ICU infections. Gram negative bacteria had been isolated on MacConkey agar using basic bacteriological...

Author(s):Lorina Badger-Emeka, Abdulrahman Abdulhadi Al-Sultan,Abdullatiff Sami Alrashed, Mohammed Sami Alhaddad, Afnan khalifah AlBarjas

Article in Press

Evaluation of starter culture fermented sweet potato flour using FTIR spectra and GCMS Chromatogram

Starch is the major component of cereal grains and starchy foods, and changes in its biophysical and biochemical properties (e.g., amylose, amylopectin, pasting, gelatinization, viscosity) will have a direct effect on its end use properties (e.g bread, malt, polymers). Lactobacillus brevis and Debaromyces polymorphous earlier obtained from fermented sweet poatao broth were used to ferment sweet potato and these starter...

Author(s):Ajayi O. I., Samuel A. E., Ehiwuogu-Onyibe J., Oluwole O. B., Salami T. A., Asieba G. O., Lawal A.K., and Elemo G.N.

Article in Press

Anti-dermatophytic activity of hexane extracts of Azadirachta indica A. Juss.

Background: Dermatophytes cause superficial fungal infections that pose public health problem to man and animals. Long term treatment with antifungal agents is required to control these infections. Various parts of Azadirachta indica are claimed to have significant medicinal value in treatment of infections especially ringworm. Objective: To determine the antifungal activity of the oil and fractions against dermatophytes...

Author(s):Ungo-kore H.Y., Ibrahim Y.K.E, Tytler B.A.

Article in Press

Prevalence and genetic diversity of the strains of Bacillus cereus groups in food for infants and young children in México

The aim of this study was to determine the prevalence and genetic diversity of the strains of Bacillus cereus groups isolated in México from foods for infants and young children. A total of 94 foods from a single commercial brand were analyzed to find B. cereus through a pre-enrichment method of colonial morphology in agar mannitol yolk polymyxin. Specific colonies were selected to be analyzed by PCR determining the...

Author(s):Adame- Gomez Roberto, Guzman- Guzman Kevin-Del-Angel, Vences- Velazquez Amalia, Leyva- Vazquez Marco- Antonio, Muñoz- Barrios Salvador and Ramirez- Peralta Arturo

Article in Press

Antimicrobial resistance pattern of Acinetobacter spp. isolated from clinical samples in a tertiary care hospital at Madinah, Saudi Arabia

Multidrug resistance, in rapidly spreading bacterial species, is a matter of concern in health care units. Samples (6840) collected from a Saudi hospital in Madinah, were screened for Acinetobacter spp. and studied for frequency, gender distribution, and seasonal variations besides antimicrobial resistance pattern. Acinetobacter strains represented 5.5% of the clinical isolates from different sources. Of these, 63 % were...

Author(s):Samah Ghanem, Hatem M. El Shafey, and Nikhat Manzoor

Article in Press

Efficacy of plasma micro broth dilution assay for antifungal susceptibility testing of Candida albicans

Fungal infection and antifungal drug resistance especially in the treatment of Immuno suppressive syndromes, has created an increased demand for reliable and affordable methods of in vitro testing of antifungal agents that can assist in their diagnosis. This study aimed to investigate antifungal susceptibility testing method that is reliable, flexible, affordable, accurate, cheap and less time consuming in order to reduce...

Author(s):Fon, E.F; Oko, A.O; Chia P, N; Yongabi, K.A

Article in Press

Low accuracy of the McFarland method to account bacterial populations

The McFarland method is designed to estimate bacterial concentrations by means of a turbidity scale (absorbance) which consists of a series of tubes, previously calibrated, and with an optical density produced by the precipitation of barium sulphate. This absorbance is compared to bacterial populations. The most used absorbance is the one corresponding to 0.5 on that scale, which assumes a population of 1.5x108 cfu / mL...

Author(s):Lozano Guzmán Eduardo, Beatriz Santos Ramirez, Cervantes Flores Maribel, María Guadalupe Nieto Pescador, Francisco Javier Moreno Cruz

Article in Press

Internal Transcribed Spacers (ITS) based identification of Trichoderma isolates and biocontrol activity against Macrophomina phaseolina, Aspergillus niger and Meloidogyne incognita

Ten Trichoderma isolates were isolated from different locations in Egypt. Amplification and sequencing of internal transcribed spacers (ITS) was employed to identify Trichoderma isolates that exhibited from 99 to 100% identity with three species of Trichoderma; Trichoderma harzianum, Trichoderma asperellum and Trichoderma longibrachiatum. The biocontrol activity of Trichoderma isolates against Macrophomina phaseolina,...

Author(s):Khalid S Abdel-lateif and Ramadan A Bakr

Article in Press

Assessment of dominant bacterial strains isolated from Ntoba mbodi, an indigenous African alkaline-fermented food, and their potential enzyme activities

Ntoba mbodi is a stable and long-life alkaline-fermented food and the domestic-scale production depends upon microorganisms from the local environment. Previous studies on bacteria in Ntoba mbodi have reported the presence of Bacillus related species and other bacterial taxa. But the abundance of these bacteria in Ntoba mbodi still needs to be determined to assess their ecological importance in this particular...

Author(s):Didine Priscilla Moutou-Tchitoula, Etienne Nguimbi, Stéphanie Giusti-Miller, Philippe Mora, Simon Charles Kobawila, Edouard Miambi

Article in Press

Study on bovine mastitis with isolation of bacterial and fungal causal agents and assessing antimicrobial resistance patterns of isolated staphylococcus species in and around Sebeta town, Ethiopia

Background: A cross-sectional study was conducted from December 2016 to May 2017 in and around Sebeta town with aim of assessing the prevalence of mastitis, isolation of aerobic bacterial and fungal causal agents and assessing antimicrobial resistance pattern of isolated Staphylococcus species in dairy cows. Results: From a total of 383 dairy cows, 220 (57.4%) were found to be positive for mastitis of which 10.4% were...

Author(s):Tesfaye Bekele, Matios Lakew, Getachew Terefe, Tafesse Koran, Abebe Olani, Letebrihan Yimesgen, Mekdes Tamiru and Tilaye Demissie

Article in Press

Isolation and identification of Escherichia coli, Salmonella and Pasteurella from holding grounds of live-bird markets at Addis Ababa, Ethiopia

A cross-sectional study was conducted to isolate and identify of Escherchia coli, Pastuerella multocida, Salmonella gallinarum and Salmonella pullorum from the holding grounds of five purposively selected Addis Ababa live bird markets from November 2016 to May 2017 using bacterial culture, grams staining and biochemical testing. A total of 90 pooled fecal samples were collected as both deep (35) (bottom layer of the feces)...

Author(s):Surra Gebeyehu, Dereje Tulu and Chaluma Negera

Article in Press

Isolation of a Lactobacillus strain from aguamiel and preliminary characterization of its antimicrobial components

The aim of the study was to characterize the antimicrobial components produced by Lactobacillus paracasei KSI. In the study was isolated and identified a L. paracasei KSI strain from aguamiel using a 16S rRNA, hsp, recA and rpoB genes sequencing. The antimicrobial capacity of the L. paracasei strain KSI was determined by agar double layer diffusion technique, while the antagonistic activity of the cell-free extract (E-KSI)...

Author(s):BENÍTEZ-SERRANO Juan Carlos, MARTÍNEZ-PÉREZ Laura, ROSAS-MURRIETA Nora Hilda, BRAMBILA-COLOMBRES Eduardo Miguel, HERNÁNDEZ-CASTRO Rigoberto, ROSALES-PÉREZ Mónica, AGUILAR-ALONSO Patricia

Article in Press

Biotechnological and industrial applications of enzymes produced by extremophilic bacteria. A mini review

Extremophilic bacteria are important groups of extremophilic organisms that have been studied during the two decades. The demand for enzymes from extremophilic bacteria has increased considerably due to its potential industrial application and great diversity and can work under extreme conditions. Due to their activity and stability under extreme conditions, these enzymes offer new alternatives for current biotechnological...

Author(s):Kelly Dumorné

Article in Press

Marine enzymes and their industrial and biotechnological applications. A mini review

Marine microorganisms are considered as a great source of biodiversity. The demand of marine enzymes are extensively and increasingly used in research and in various industries due to their diversity to survive under extreme conditions such as saline conditions, pH, temperature, light, atmospheric pressure, and the availability of nutrients. Despite, their huge biodiversity and benefits that present marine enzymes their...

Author(s):Kelly Dumorné and Marcia Astorga Élo

Article in Press

Effect of indigenous and effective microorganism fertilizers on soil microorganisms and yield of Irish potato in Bambili, Cameroon

Irish potato (Solanum tuberosum) is one of the world’s most consumed staple worldwide and an important crop in terms of food security in the face of population growth and increased hunger rates. Potato yields in Cameroon have often been low as a result of decrease in soil fertility. Soil fertility has often been regarded as the chemical and physical properties of soil, with the microbial aspect often being ignored. An...

Author(s):Taffouo Victor Desiré, Muyang Rosaline Fosah, Mbouobda Hermann Desiré and Fotso

Article in Press

Screening of bioactive compound, antimicrobial activity producing halophilic isolates from the saltpans of Thoothukudi district

Extreme environments harbor a number of microbes producing novel bioactive compounds. The aim of our study is to isolate and identify bioactive compound producing halophiles. Marine soil sediments were collected from the solar saltpans of Thoothukudi District, Tamil Nadu, India. Based on colony morphology, two species were isolated and identification was done by using morphological and biochemical tests. The extracts of...

Author(s):Deepalaxmi R. K. and Gayathri C.

Article in Press

Evaluation of gene xpert assay and microscopic examination for detection of Mycobacterium tuberculosis in sputum at Port Harcourt, Nigeria

The re-emergence and increase in the prevalence of tuberculosis cases and multidrug resistant strains is of public health concern. The conventional Acid Fast Bacilli detection tool is weak in the current disease trend especially in children, patients with human immunodeficiency virus (HIV) and low bacterial load. The aim of this research was to assess effectiveness of the Gene Xpert MTB/RIF assay and Microscopy in...

Author(s):Confidence Kinikanwo Wachukwu, Stella Paulinus Okwelle, Easter Godwin Nwokah and Ollor Amba Ollor

Article in Press

Ameliorative effect of imipenem on pulmonary damage caused by extended spectrum β-lactamase (ESBL) producing bacteria isolates from non- human source

Acute lung infection induced by Extended Spectrum β-Lactamases (ESBL) producing isolates was determined by measuring inflammatory mediators; malondialdehyde (MDA), myeloperoxidase (MPO) and nitric oxide (NO). The mice were randomly divided into three groups of 20 animals each. All mice were given 104 c.f.u. ml-1 of the test organism intranasally in a volume of 50 µl while holding the mouse in an upright position without...

Author(s):Afunwa, R. A., Iroha, R. I., Ugwu, M., Afunwa, E. C., Unachuchwu, M. N. and Esimone, C. O.

Article in Press

Assessment of antimicrobial resistance patterns in Escherichia coli isolated from clinical samples in Madinah, Saudi Arabia

Escherichia coli is a Gram-negative bacteria that causes various diseases, including pneumonia, urinary tract infections, and diarrhoea. The present work is an effort to study antimicrobial resistance pattern in this bacteria. Clinical samples (6840) were collected from King Fahd Hospital in Madinah, Saudi Arabia and screened for E. coli strains. Of all positive samples obtained from different clinical sources, about 3%...

Author(s):Walaa M. Saeed, Samah Ghanem, Hatem M. El Shafey and Nikhat Manzoor

Article in Press

Incidence of bacterial infection in chronic hepatitis C virus (HCV) patients with cirrhosis and association between toll-like receptor 4 D299G gene polymorphism and Gram- negative bacterial infections in the patients

This study aimed to investigate the incidence of bacterial infection in chronic hepatitis C virus (HCV) patients with cirrhosis and association between toll-like receptor 4 D299G gene polymorphism and Gram-negative bacterial infections in the patients. 100 HCV cirrhotic patients with ascites and 20 age- and sex-matched healthy subjects as control were included. Conventional culture methods were used to identify the...

Author(s):Hanan El-Bassat, Lobna Abo Ali, Atef Taha and Rasha Alm El-Din

Article in Press

Assessment of the prevalence of extended-spectrum β-lactamase producing Gram-negative bacilli at the Charles De Gaulle Paediatric University Hospital (CDG-PUH), Ouagadougou, Burkina Faso

Bacterial resistance to antibiotics is a serious concern in developing countries due to precarious hygiene conditions, inappropriate prescription as well as self-medication and free sale of antibiotics. This study was aimed to assess the prevalence of strains producing extended-spectrum beta- lactamase in the Gram negative bacteria isolated in the laboratory. The study was conducted in the Charles De Gaulle Paediatric...

Author(s):Mahamoudou Sanou, Absétou Ky/Ba, Pricille Coulibali, Marius Nagalo, Abdoul Salam Ouédraogo, Mamadou Tamboura, Dinanibé Kambiré, Cyrille Bisseye, Fidèle Bakiono, Jacques Simporé and Ramata Ouédraogo

Article in Press

Management of bacterial wilt disease of potato in coastal plains of Odisha

Bacterial wilt and brown rot caused by Ralstonia solanacearum is one of the most dreaded diseases causing 30 to 70% yield loss in India. The disease is both tuber and soil borne. A field trial was conducted following split plot design in the year 2008 to 09 and 2009 to 10 under All India Co-ordinated Potato Research Project in Central Farm, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India with...

Author(s):Biswal, G. and Dhal, N.K.

Article in Press

Isolation, identification and evaluation of mosquito entomopathogenic Bacillus species and related genera from randomly selected sites in Kenya

This study was carried out to identify specific mosquitocidal Bacillus species and related genera for future development of biopesticides in local mosquito control program in Kenya. Bacterial isolation was conducted from 100 soil samples through pasteurization method and preliminary identification conducted through phenotypical analysis. Toxicity analysis was performed through bioassays and lethal concentrations (LC) were...

Author(s):Samuel Karungu, Doudou Huang, Evans Atoni, Cecilia Waruhiu, Bernard Agwanda, Xiaomin Hu and Zhiming Yuan

Article in Press

Influence of subminimal inhibitory concentrations of some bioactive compounds on the biofilm formation ability and virulence factors of multiple drug resistant Acinetobacter baumannii clinical isolates

The objective of this study was to assess the impact of subminimum inhibitory concentrations (sub-MICs) of chosen antimicrobial agents (Amikacin, imipenem, benzalkonium chloride, and chlorhexidine) and natural product (garlic) on biofilm formation ability, bacterial adherence and invasion. Susceptibility profiles of 50 non-repetitive Acinetobacter baumannii clinical isolates to eight antibiotics were investigated. MIC of...

Author(s):Mona H. Mahmoud, Magdy A. Amin, Rania I Shebl, Aly F. Mohamed and Mai M. Zafer

Article in Press

Physiochemical factors affecting in vitro growth of Pasteurella multocida

Pasteurella multocida causes fowl cholera (FC), a contagious bacterial disease of domestic and wild avian species. It is a Gram negative coccobacilli causing acute highly fatal septicemia with high morbidity and mortality rate. Control of fowl cholera is primarily performed by good management practice and vaccination in areas where disease is endemic. For a quality vaccine, high density growth of P. multocida along with...

Author(s):Mehmood M. D., Zia S., Javed F., Gul M., Ashraf M. and Anwar H.

Article in Press

Antimicrobial activity of crude extracts of endophytic fungi from Oryctanthus alveolatus (Kunth) Kuijt (Mistletoe)

The use of antibiotics has unleashed high bacterial resistance. This outcome triggered the urgent need for effective new antibacterial agents to treat infectious diseases. A promising source for the production of antibiotics and several other bioactive substances are endophytic fungi. These microorganisms inhabit in and bring benefits to living plant tissues. Thus, the aim of this paper was to know the endophytic fungi...

Author(s):Sanay Feitosa Lima Ribeiro, Armando da Costa Garcia, Hathyla Eduarda Dias dos Santos, Quimi Vidaurre Montoya, Andre Rodrigues, João Marcos de Oliveira and Camila Martins de Oliveira

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