African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002

AJB Articles in press

Article in Press

Technological properties and sugar tolerance performance of palm wine yeasts isolated from parts of Nsukka, Nigeria

The ability of yeasts to survive and produce significant ethanol in high sugar medium and high ethanol environment is essential for the use of such yeasts in industrial and edible ethanol production. Four Saccharomyces spp. strains ( ) isolated from palm wine and an industrial strain were studied in high glucose medium for ethanol production and ability to survive in high sugar medium. Saccharomyces cerevisiae produced...

Author(s):Nsikak U. Stephen, J. Obeta Ugwuanyi and Ukponobong E. Antia

Article in Press

Genetic diversity among elite chickpea (Cicer arietinum L.) varieties of Ethiopia based on inter simple sequence repeat markers

The inter simple sequence repeat (ISSR) marker was used in this study for genetic fingerprinting and identification of released chickpea varieties and for the determination of the genetic relationships among these cultivars. A total of 19 released chickpea varieties were subjected to ISSR analysis with the objective of evaluating the genetic diversity among the cultivars. A total of 20 ISSR primers were initially screened...

Author(s):Zerihun Jida, Shiferaw Alemu and Destaw Mullualem

Article in Press

Generation of electricity using watermelon waste (Citrullus lanatus) through a single chamber mediator-less microbial electrochemical cell

The use of fossil fuel for the generation of electricity pollutes the environment. The noxious gases that are expelled from it result in global warming just like the careless disposal of organic waste materials that destroy the aesthetic beauty of the environment. This research therefore aims to convert such organic wastes into electricity. The waste of watermelon fruit (Citrullus lanatus) collected from a fruit garden...

Author(s):Akpotayire, S. I., Kalagbor, I. A., Miikue-Yobe, T. F. B. and Nyiador, P.

Article in Press

Activation of TLRs/NF-κB signaling pathway and production of diverse cytokines in the incidence and development of invasive pulmonary aspergillosis

Aspergillus fumigatus is a common saprophytic fungus in the air. It can cause a severe infectious disease called invasive pulmonary aspergillosis (IPA). To establish the activation of TLRs/NF-κB signaling pathway in invasive pulmonary aspergillosis (IPA) model of wild type mouse, three groups of mouse were used in the study; the normal mice; mice infected with A. fumigatus conidia; IPA mouse infected with A. fumigatus...

Author(s):MA Guangqiang, WU Su-fang, LUO Hong-dan, XIE Wei-hua, LONG KAI, SU Ming-sheng and XIE Xiao-mei

Article in Press

Effect of seasonal variations on the yield of essential oil and antioxidant of Achillea fragrantissima (Forssk) Sch. Bip

Temperature stress is becoming the major concern for plant scientists worldwide due to climate change. Temperature stress has devastating effects on plant growth and metabolism. The aim of the study was to investigate the effect of climatic seasonal change on the yield and composition of essential oil of the plant, Achillea fragrantissima. The gas chromatography-mass spectrometry (GC-MS) used to analyze the essential oil...

Author(s):Eman Elsharkawy, and Nahed Nor Edine

Article in Press

Bio-prospecting of macro-algae for potential industrial dyes

Synthetic dyes have been used for different purposes in various fields, but their continued usage has presented both environmental and health challenges. Therefore, alternative safer dye sources are inevitable. Algae, a pigment containing aquatic eukaryote ranging from microscopic single celled to multicellular forms have the potential for exploration and usage in different socio-economic sectors. The aim of this study was...

Author(s):Christine W. Njiru, Huxley M. Makonde, John M. Kahindo, Edwin O. Matoka and Charles M. Matoka

Article in Press

In vitro inhibitory potentials of aqueous and ethanol extracts of Hyptis suaveolens on fungi associated with postharvest spoilage of Brassica oleracea

The inhibitory potential of Hyptis suaveolens leaf and root extracts on fungi associated with postharvest spoilage of cabbage (Brassica oleracea var. capitata) was examined. Collection of diseased cabbage was done in sterile sample bags. Isolation of fungi was carried out by inoculating small sections of diseased tissues excised from the cabbages unto Petri-dishes containing Acidified Potato Dextrose Agar (APDA). The...

Author(s):Chidiebere R. Agbawodike, Ayodele A. Sobowale, Jonathan S. Gbolagade and Ifeanyi Oscar N. Aguzie

Article in Press

Antibacterial saponins from the leaves of Polyscias fulva (Araliaceae)

Saponins are a major family of secondary metabolites that occur in a wide range of plant species. Bioassay - guided fractionation of extract of the leaves of Polyscias fulva led to the isolation of three known saponins named, 3-O-α-L-arabinopyranosyl-hederagenin (1), 3-O-[α-L-rhamnopyranosyl(1-2)-α-L-arabinopyranosyl]-hederagenin (2) and...

Author(s):Winnie Cherotich Maritim, Isabel Nyokabi Wagara and Josphat Clement Matasyoh

Article in Press

Genetic variability, heritability and genetic advance for green pod yield and yield related traits in chili (Capsicum annum L.) accessions in Ethiopia

A field experiment was conducted to study the genetic variability, heritability and genetic advance for 12 quantitative characters in chili (Capsicum annum L.) accessions in Ethiopia, during the off-season period of 2012/2013 under flood irrigation at the Adet Agricultural Research Centre field. The experiment was conducted using Randomized Complete Block Design with three replications and a total of 25 accessions and four...

Author(s):Tadesse Tilahun, Yohannes Petros and Yigzaw Dessalegn

Article in Press

Alternative markers linked to the Phg-2 angular leaf spot resistance locus in common bean using the Phaseolus genes marker database

Common bean (Phaseolus vulgaris) is a rich source of protein, vitamins, and micronutrients and is an important crop for food security throughout Latin America, Asia, and Africa. Among tropical and subtropical regions of the world, where the majority of beans are grown, yield losses due to the pathogenic fungus Pseudocercospora griseola, causing angular leaf spot (ALS), can be as high as 80%. The strategic use of...

Author(s):Tamara Miller, Paul Gepts, Stephen Kimmo, Esther Arunga, Luseko Amos Chilagane, Susan Nchimbi-Msolla, Annet Namusoke, Annet Namayanja and Yayis Rezene Tedla

Article in Press

Effects of temperature and time on oil extraction from some Nigerian indigenous fresh water microalgae species

This study determined the effects of temperature and time on oil extraction from indigenous freshwater microalgae: Dictyosphaerium, Chlorella, Desmodesmus and Cosmarium species, cultured in 15 2-L column photobioreactors (PBR) (three per species). Growth and specific growth rates for the mixotrophic>autotrophic cultivation of the species were determined. Oil was extracted from the dried microalgae species at temperatures...

Author(s):Felix Uzochukwu Asoiro, Wilfred Ifeanyi Okonkwo, Clement Onyeaghala Akubuo, Nkechinyere O. Nweze and Cosmas Ngozichukwu Anyanwu

Article in Press

Atomic absorption spectroscopic evaluation of some heavy metal contents in tomato (Lycopersicon esculentum Mill) fruit and Ribu River water used for irrigation at Fodugora Farm, Weliso Town, Ethiopia

The levels of some heavy metals in mature fruits of tomato (Lycopersicon esculentum Mill) grown at Fodugora Farm, Weliso Town (Ethiopia) with Ribu River effluent water, used for its irrigation, have been determined using flame atomic absorption spectroscopic (FAAS) technique. Whereas, the levels of Fe, Zn and Pb in tomato fruits were: 34.05, 4.05, and 1.32 mg/L respectively, their concentrations in the river effluent water...

Author(s):Gera Techane, O. P. Yadav and LokeshYadav

Article in Press

Diversity and genetic identity of pineapple [Ananas comosus (L.) Merr.] in Tanzania based on microsatellite markers

Pineapple [Ananas comosus (L.) Merr.] is an important fruit crop cultivated in Tanzania. However, the knowledge on genetic diversity of the pineapple cultivars grown in Tanzania is limited. This study was aimed at determining the genetic diversity and identity of pineapple cultivars from different growing regions in Tanzania using microsatellite markers also known as simple sequence repeat marker-simple sequence repeat...

Author(s):Abdalah Makaranga, Miccah Songelael Seth, Ayub Ndee, Emmarold E. Mneney, George Mbwambo, Kusirieli Lema, Aron Godfrey, Lilian Mrema, Andrew Kachiwile, Emmanuel Mrema and Theodosy J. Msogoya

Article in Press

Microsatellites markers associated with resistance to flower bud thrips in a cowpea F2 population derived from genotypes TVU-123 and WC36

Breeding for resistance to flower bud thrips (Megalurothrips sjostedti) in cowpea has been hindered by the quantitative nature of resistance. To identify simple sequence repeat (SSR) associated with resistance to flower bud thrips that could be used for marker-assisted breeding, a F2 population was generated from a cross between genotypes TVU-123 (resistant) and WC36 (susceptible). The population was evaluated for thrips...

Author(s):Symphorien Agbahoungba Jeninah Karungi, Kassim Sadik, Paul Gibson, Richard Edema and Achille E. Assogbadjo

Article in Press

Molecular diagnosis of phytoplasma transmission from zygotic embryos to in vitro regenerated plants of coconut palm (Cocos nucifera L.)

The aim of this study was to investigate the transmission of the lethal yellowing disease (LYD) of coconut tree caused by a phytoplasma from the zygotic embryo to the regenerated plantlet in vitro. From a total of 30 trees, 150 mature coconut nuts where harvested. These nuts were used to extract 150 zygotic embryos. From this package, 96 zygotic embryos were used to regenerate 96 young coconut seedlings in vitro and the 54...

Author(s):DARAMCOUM Wentoin Alimata Marie Pierre, KONAN Konan Jean-Louis, YAO Saraka Didier Martial, YAIMA Arocha Rosete, KOFFI Eric-Blanchard Zadjéhi, YOBOUE Koffi, KOUASSI Kan Modeste, KOUADJO Claude Ghislaine, KOFFI Edmond, KOFFI Kouadio Kan Ghislain and N’GUETTA Assanvo Simon-Pierre

Article in Press

In vivo evaluation of aggressiveness, pathogenicity and patulin accumulation by Penicillium expansum strain isolated from Algerian apples

Penicillium expansum is one of the most important postharvest pathogens of apple fruit worldwide. It causes blue mold decay, one of the important economical diseases of apple fruits and it is the main producer of patulin. The main objective of this study is to evaluate the aggressiveness and patulin production of an isolated strain from apples produced in the East of Algeria. The strain was isolated, screened for its...

Author(s):Hamida KROUMA, Radia CHERFIA, Atef JAOUANI, Amor MOSBAH, Soukaina ABDELWAHAD, Mounira KARA ALI and Noreddine KACEM CHAOUCHE

Article in Press

Assessment of morphological characteristics among upland rice (Oryza sativa and Oryza glaberrima) germplasm

Rice is an important staple food crop that feeds over half of the global population and has become the cereal that provides a major source of calories for the urban and rural poor in Africa. This work aimed to evaluate the morphological of rice (Oryza sativa and Oryza glaberrima) germplasm. In the present study, 14 quantitative traits were used across 48 accessions or genotypes obtained from Central Agricultural Research...

Author(s):Zogbo Luther, Richard Akromah, David P. Tokpah and Zipporah Page

Article in Press

Molecular epidemiological studies on Trypanosoma evansi type A and type B in camels (Camelus dromedaries) from five different regions of Saudi Arabia using the ITS1 rDNA and RoTat 1.2 VSG gene

Trypanosoma evansi is the most widespread of the pathogenic salivarian trypanosomes and cause a serious disease called surra that affects domestic animals such as camels and horses in tropical and subtropical countries and often leads to reduced productivity and economic losses. Therefore, the objectives of the present study were to determine the prevalence rates of trypanosomiasis using polymerase chain reaction (PCR)...

Author(s):Abdullah D. Alanazi

Article in Press

Comparative survey of three processes used for the extraction of total phenol content and total flavonoid content of Anacardium occidentale L. and the assessment of its antioxidant activity

Three processes were used for the extraction of total phenol and total flavonoid contents of Anacardium occidentale L. and to determine its antioxidant activity. Whatever the extraction process was, the total flavonoid and total phenol contents were of the same order. With the microwave-assisted extraction (MAE), 666.07 mg gallic acid equivalent (GAE)/100 g dry matter (DM) of total phenol content was obtained from the stem...

Author(s):MADJITOLOUM BETOLOUM Salomon

Article in Press

Genetic diversity assessment of yams (Dioscorea spp.) from Ethiopia using inter simple sequence repeat (ISSR) markers

The genetic diversity and relationships of 70 accessions of yam belonging to Dioscorea cayenensis/Dioscorea rotundata complex (55), Dioscorea bulbifera (13) and Dioscorea alata (2, as a reference) were assessed using six inter simple sequence repeat (ISSR) primers. DNA was extracted from a bulk of two plants per accession using a modified cetyl trimethyl ammonium bromide (CTAB) method. Six ISSR primers amplified 77...

Author(s):Kedra Mohammed Ousmael

Article in Press

Study of secondary metabolites produced by Aspergillus flavus and evaluation of the antibacterial and antifungal activity

The objective of this study was the analysis of the secondary metabolite products and evaluation of antibacterial activity of Aspergillus flavus. Bioactives are chemical compounds often referred to as secondary metabolites. Thirty one bioactive compounds were identified in the methanolic extract of A. flavus. The identification of bioactive chemical compounds is based on the peak area, retention time, molecular...

Author(s):Ghaidaa J. Mohammed, Imad H. Hameed and Sabreen A. Kamal

Article in Press

Opaque-2 Gene and Beta Carotene Content in 20 Yellow-, Red and Purple-Kernelled Maize (Zea Mays L.) Accessions in Ghana.

Twenty yellow-, red- and purple-kernel maize accessions were collected from three geographic regions in Ghana for the study. The objective was to determine the presence of opaque -2 gene and -carotene content of the grains as a means of assessing nutritional quality. Variability in -carotene content was determined by HPLC while presence of opaque 2-gene was determined by a light box. Significant variations were found in...

Author(s):GILBERT ANSAH and KOJO AHIAKPA

Article in Press

Microbial machinery based synthesis of nanoparticulate hydroxyapatite using Serratia marcescens and its characterization for human bone implant

Hydroxyapatite (HAp), chemically and compositionally similar to the material presents in bone and has a unique biocompatibility which makes it an attractive material for bone implant. We aimed at studying the physical and chemical properties of HAp synthesized by Serratia sp. The total of 58 strains was isolated from 354 samples obtained from clinical and environmental sources like urine, sputum, soil, water and plant...

Author(s):Rathinasamy Subashkumar, MIDHUSHA JOHNY and Nandhagopal Gayathri

Article in Press

Fatty acids profiling of the seed oils of some varieties of peanut (A.hypogaea L.) towards the development by GC-MS analysis.

Twelve fatty acids were identified during maturity in the wild Tunisian and cultivar peanut kernels. These fatty acids include C16:0 (palmitic acid), C18:0 (stearic acid), C18:1 (oleic acid), C18:2 (linoleic acid), C19:0 (nonadecanoic acid), C20:1 (gadoleic acid), C20:0 (arachidic acid), C22:1 (erucic acid), C22:0 (behenic acid), C23:0 (tricosanoic acid), C24:0 (linoceric acid), and C26:0 (cerotic acid). Three fatty acids...

Author(s):Aicha. O. CHERIF, Mhamed BEN MESSAOUDA, Habib KALLEL, Claude PEPE

Article in Press

Isolation of a novel strain Bacillus sp.in producing neutral and heat stable beta-1,4 endoglucanase activity

Neutral a β-1-4 - endoglucanase was produced by aerobic bacteria a newly strain Bacillus sp .RL. Isolated from soil, Chong Qing province, China. In the present study the bacteria produce a significant amount of enzymes after 72 hrs of fermentation CMC medium were 0.1852 U/ml of endoglucanase, 0.5814 U/ml of FPase activity and 2.9940 mg/ml of extracellular protein. These strains showed heavy growth and liquefaction of 7.0,...

Author(s):Rahman Laibi Chelab, Dan Dan Wang and Xingyong Yang

Article in Press

Phytochemical profile and biological activities of Momordica charantia L. (Cucurbitaceae): A review

This study discusses the bioactive composition, supercritical fluid extraction and biological activities of Momordica charantia L. from the last five years. Numerous compounds that have been identified in the extracts of M. charantia, including phytosterols, terpenoids, fatty acids, phenolic compounds, phenolic acids and flavonoids were also discussed. Although, several studies reported the use of organic solvents in the...

Author(s):Mozaniel Santana de Oliveira, Wanessa Almeida da Costa, Fernanda Wariss Figueiredo Bezerra, Marilena Emmi Araújo, Gracialda Costa Ferreira and Raul Nunes de Carvalho Junior

Article in Press

In vitro propagation as a viable alternative to the continuous regeneration of three Eucalyptus species (Eucalyptus camaldulensis, E. gomphocephala and E. maidenii)

The objective of this work was to propagate three species of Eucalyptus: Eucalyptus camaldulensis, Eucalyptus gomphocephala and Eucalyptus maideni on a large scale using microcutting technique. For the test of microcutting, from in vitro germination, the best rate of multiplication of 4.75 were obtained for E. camaldulensis on a medium containing 1 mg/L 6-benzylaminopurine (BAP) and 1 mg/L -aminoisobutyric acid (AIB) in...

Author(s):Hamrouni Lamia, Hanana Mohsen, Derbali Fedia, Hwass Haifa , Khouja Mohamed Larbi and Bouzid Sadok

Article in Press

Cloning and expression of recombinant human insulin gene in Pichia pastoris

The main goal of this current study was the cloning and expression of the human insulin gene in Pichia pastoris expression system, using genetic engineering techniques and its treatment application. Total RNA was purified from fresh normal human pancreatic tissue. RNA of good quality was chosen to obtain a first single strand cDNA. Human preproinsulin gene was amplified from cDNA strand using two sets of specific primers...

Author(s):Rafid A. Abdulkareem, Samah A. Jassam and Majid J. Jabber

Article in Press

Genetic diversity of antibiotic and plant extract resistant Staphylococcus aureus isolated from hospitalized patients in Ekiti State, Nigeria

This study aimed to determine the genetic diversity of antibiotics and plants extract resistant Staphylococcus aureus using molecular technique. A total of 106 human clinical samples were collected from patients in three different hospitals within Ekiti State. Microbiological and molecular analyses were performed using standard methods. Antimicrobial susceptibility test with common antibiotics and plant leaves extracts was...

Author(s):Egbebi Adeola Oluwagbemileke, Oyama Maslhanty Obem and Fadare Olalekan Shadrach

Article in Press

Physico-chemical properties of Brazilian biomass: Potential applications as renewable energy source

Biomass thermal behavior under different atmosphere: combustion, pyrolysis (N2) and typical oxy-fuel combustion (60 and 80% CO2) were investigated by thermal analysis (TG/DTG and DTA). Other techniques such as X-Ray, Fourier Transformed Infrared (FTIR), Wettability, Scanning Electron Microscopy (SEM) and Inductive Couple Plasma - Optical Emission Spectrometer (ICP-OES) were also used to characterize the samples. Five...

Author(s):Glauber CRUZ, Carlos E. M. BRAZ, Ivonete ÁVILA and Paula M. CRNKOVIC

Article in Press

Establishment of high frequency regeneration protocol for Lycopersicon esculentum from leaf explant using seaweed extracts

The ultimate aim of this study is to standardize the conditions for in vitro organogenesis of tomato using plant growth regulators, organic elicitors, polyamines and seaweed extracts. Initially excised leaf explant was cultured on to the medium containing different concentrations of auxins. The best responded auxin was tested in combination with efficient cytokinins (Kinetin [Kin], 6-Benzylaminopurine [BAP] and Thidiazuron...

Author(s):S. Vinoth, P. Gurusaravanan, S. Sivakumar, G. Siva, G. Premkumar and N. Jayabalan

Article in Press

Characterization of lactic acid bacteria isolated from dromedary raw milk in Syria

Lactic acid bacteria (LAB) are considered a micro flora of Syrian camel milk. A total of 235 isolates of LAB were obtained from 63 camel milk samples, which have been collected from producing areas of different regions of Syria during 2009 and 2012. The isolates were cocci and rods, gram-positive and catalase-negative. 132 isolates were identified as cocci including 76 isolates belonging to Enterococcus (45 were...

Author(s):Ayman Al-Mariri, Ahed Abou Younes and Laila AL-Hallab

Article in Press

Effects of aqueous seed extracts of Mucuna sloanei (Fabaceae) on body weight and some biochemical parameters of Rattus novergicus

Mucuna sloanei is an annual leguminous plant widely used among the various ethnic groups in Nigeria. The effects of aqueous M. sloanei seed extract on the body weight and some biochemical parameters of 48 normal male Rattus novergicus (albino rats) were investigated for 28 days. The rats were divided into control group (A) which received distilled water and treatment groups (B, C and D) that received oral administration of...

Author(s):Ugwu, Godwin C., Ejere, Vincent C., Okanya, Chinagorom L., Omeje, Joy N., Egbuji, Jude V., Onu, Martina C. and Chukwuka, Christian O.

Article in Press

In silico analysis of antimicrobial eupalitin isolated from Ageratina adenophora using docking method

The present study evaluated the efficacy of eupalitin compounds against the target protein, dihydrofolate reductase of bacterial and fungal pathogens using in silico approach. Structure based docking studies was performed using Schrodinger (Version: 1.8) tool to retrieve potential candidates with high affinities binding against eupalitin models with several targeted proteins repositories. The specific targets were selected...

Author(s):Suganya Mani, Kavitha Shivaji, Karthik Natesan, Ashaq Hussain Bhat, Mythili Gnanamangai Balasubramanian and Ponmurugan Ponnusamy

Article in Press

In vitro screening of traditional rice varieties of Assam, India for drought tolerance

Eight traditional rice varieties viz., bora, prosad bhog, kola joha, helash bora, salihoi bao, kola amona, ronga bora and sok-bonglong along with ‘sahbhagi dhan’ (drought tolerant) and ‘IR64’ (drought sensitive) were studied invitro under two different drought stress conditions. Different parameters studied were germination percentage, shoot length and root length. Drought stress was produced by ½ Murashige and Skoog (MS)...

Author(s):Shamsun Nahar and Bhaben Tanti

Article in Press

Potential application of nutrigenomics in livestock: Lesson from developed countries

Livestock performance is affected by genotype and environment. Nutrition is one of the greatest environmental determinants. Genes function according to the metabolic signals received by the nucleus from hormones and nutrients. Currently, nutrigenomics, which encompasses the three crucial omics stages (transcriptomics, proteomics and metabolomics), is emerging in the area of livestock to develop feeds that can be...

Author(s):Bruh Asmelash Gebremikael, Mahlet Dawit Teweldebrihan and Haftom Brhane Mesfin

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