African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002

AJB Articles in press

Article in Press

THE TECHNOLOGICAL INFLUENCE OF THE DIFFERENT METHODS OF EXTRACTION ON THE INITIAL CONCENTRATION OF THYMOL IN THE ESSENTIAL OIL OF THYME (THYMUS SATUREOIDES)

The present study deals with the influence of the technological factor related to the technique used for the extraction of essential oil of thyme, on the his chemical composition, in particular, the concentration of thymol as the principal chemo-typical active substance of this variety of thyme from the Moroccan High Atlas. The working protocol includes the extraction of the essential oil by three different methods and...

Author(s):Imad eddine Elkacimi, Yahia Cherrah and Katim Alaoui

Article in Press

Genetic Diversity in Radix Species from the Middle and South of Iraq Based on Simple Sequence Repeats

Ten newly developed microsatellite loci were isolated from an AG20 and CAG20 enriched genomic DNA library of Radix sp. from Iraq. The simple sequence repeats (SSR) comprised of 76.0% di-, 20.0% tri- and 4.0% tetra-nucleotide repeats. The number of alleles for all 10 loci ranged from 4 to 18, with a mean of 11.3 alleles per locus. The observed (Ho) and expected heterozygosity (He) values varied from 0.286 to 0.927 (mean...

Author(s):Ali BM Al-Waaly, Kanokporn Triwitayakorn, Nattaya Srisawad, Duncan R Smith, Mohammad K Mohammad and Supajit Sraphet

Article in Press

Phytochemical and proximate composition of cucumber (Cucumis sativus) fruit from Nsukka, Nigeria

Cucumber (Cucumis sativus L.) is very common, cultivated throughout the world and often eaten as a raw vegetable without cooking. In this study, the phytochemical and proximate compositions of cucumber were investigated. Quantitative phytochemical analysis of the homogenate of C. sativus fruit showed that reducing sugars (574.36 ± 3.88 mg/g) was highest amount when compared to other phytochemicals, alkaloids (2.22 ± 0.96...

Author(s):Uzuazokaro Mark-Maria Agatemor, Okwesili Fred Chiletugo Nwodo and Chioma Assumpta Anosike

Article in Press

Effect of spices on the microbiological quality of Hibiscus sabdariffa (zobo) drink and molecular characterization of the associated spoilage organisms

Hibiscus sabdariffa drink (zobo) was preserved with three concentrations of cloves (Syzygium aromaticum), ginger (Zingiber officinale) and mixture of cloves and ginger, respectively while the control had no spice. The bottled H. sabdariffa drink was stored at 28 ± 2°C for 16 days. The total bacteria and fungi counts were determined every 48 h during storage. Bacterial and fungal growths were not detected in H. sabdariffa...

Author(s):Adeoye, B. K., Oyewole, O. B. , Idowu, M. A., Obadina, A. O., Ani, I. F. and Ngozi, E. O.

Article in Press

Degradation kinetics of 2,4-dichlorophenoxyacetic and atrazine by Trametes versicolor (L.:Fr.) Pilát

The degradation kinetics of different sources of 2,4-dichlorophenoxyacetic (2,4-D) and atrazine by the natural strain Mo008 of the basidiomycete fungus Trametes versicolor (L.:Fr.) Pilát was studied, knowing that in this process the strain used produces an enzyme complex composed of manganese peroxidase (MnP), lignin peroxidase (LiP) and laccase, and the response on the global consumption rate and yield is dependent on the...

Author(s):Ernesto Hernández Mendieta, Dagoberto Guillén Sánchez, Iran Alia Tejacal, Victor López Martínez, María Andrade Rodríguez, Edgar Martínez Fernández, Manuel Huerta Lara, Catalina Rubio Granados

Article in Press

Role of Toll-like receptor 4 in regulating nucleotide-binding oligomerization domain2 (NOD2)-mediated inflammatory responses through Aspergillus fumigatus conidia stimulation in RAW264.7 macrophages

Nucleotide-binding oligomerization domain2 (NOD2) of the NOD-like receptors (NLRs) and Toll-like receptor4 (TLR4) of the TLRs are major pattern-recognition receptors. They sense different microbial pathogens and play important roles in innate immunity. Current in vivo and in vitro studies have shown that TLR4 and NOD2 act in anti-Aspergillus fumigatus infection. Herein, the cross-interaction between membrane-bound TLR4 and...

Author(s):MA Guang-qiang, WU Su-fang, LUO Hong-dan, XIE Wei-hua, LONG Kai, SU Ming-sheng and XIE Xiao-mei

Article in Press

Identification of secondary metabolites by GC-MS in native strains of Trichoderma spp.

Trichoderma isolates were collected from agricultural soil. The production of Trichoderma metabolites was performed using 150 ml of potato broth supplemented with Sucrose (5%) and Yeast Extract (5%). The inoculation was performed by the addition of a 4 mm disk of active mycelium, and allowed to grow at 25 °C ± 2 °C, 12 h light / dark on a rotary shaker at 150 rpm for three weeks. Subsequently, the extraction of metabolites...

Author(s):Mrs Martínez Hadassa Yuef

Article in Press

HETEROZYGOTE POLYMORPHISMS OF ARG16GLY AND GLN27GLU ADRB2 GENE IS RISK PROTECTIVE FOR OBESITY IN JAVANESE POPULATION OF INDONESIA

The relationship of obesity and insulin resistance is influenced by various factors, including genetics. ADRB2 gene polymorphisms of Arg16Gly and Gln27Glu showed inconsistent results toward obesity and insulin resistance in some populations. This study aimed to investigate the frequency of the genetic polymorphisms of Arg16Gly and Gln27Glu of the ADRB2 gene and to analyze risk factors for obesity and insulin resistance in...

Author(s):Nazula Rahma Shafriani, Pramudji Hastuti, Ahmad Hamim Sadewa

Article in Press

Study of variation of the concentration of thymol and carvacrol in the essential oil of thymus satureoides during aging in accelerated time lasting 6 months

The purpose of this work is the stability study of two chemo-typical active ingredients (thymol and carvacrol) of the essential oil of thyme “thymus satureoides” collected in the region of Azilal, Morocco. The working protocol was structured around the following main axes: The supply of raw material of work, specimen identification, essential oil extraction, initial and final chemical characterization of the...

Author(s): elkacimi imad-eddine

Article in Press

Chemical Composition of Cyperus esculentus nut and Phoenix dactylifera fruit

This study investigated the chemical composition of Cyperus esculentus nut and Phoenix dactylifera fruit. The nuts and fruits were purchased as commonly sold in Wukari, Nigeria. They were cleaned, sorted for healthy parts, sun-dried and milled into fine powder. The AOAC, AAS and GC-MS methods were adopted for the proximate, mineral and phytochemical analysis respectively. The results show that Cyperus esculentus nut is...

Author(s):Chinedu Imo, Friday O. Uhegbu, Kayode A. Arowora, Chukwuma S. Ezeonu, Ifeoma J. Opara, Emmanuel E. Etim and Chigozie J. Anigbo

Article in Press

The oxidative stability of linolenic fatty acid in farmed rainbow trout (Oncorhynchus mykiss) under different storage conditions in Isfahan City of Iran

The aim of this study was to investigate the effect of different storage conditions in market (ice box, glassy refrigerator, normal refrigerator without light (natural and electric power) and freezer) on alpha linolenic acid (ALA), a nutritional value of rainbow trout (Oncorhynchus mykiss). To investigate the effect of storage duration on fatty acid quality, samples stored after 1, 3 and 5 days in refrigerator and those...

Author(s):Lakzadeh, L., Mirmohammadi, M., Amouheidari, M. and Bavarian, A.

Article in Press

pression, Purification and On-Column Refolding of Recombinant S-layer Protein from L. acidophilus ATCC 4356

One of the most significant challenges in biotechnology is technological utilization of self-assembled systems. Surface (S-) layer technology is advanced due to the intrinsic property of subunits to reassemble into two-dimensional arrays. Lactobacillus acidophilus ATCC 4356 composed of S-layer protein (SlpA) subunits which are organized into regular arrays. Herein, S-layer gene (slpA) of L. acidophilus was cloned in...

Author(s):Elaheh Sadat Hosseini , Rouha Kasra Kermanshahi , Saman Hosseinkhani and Mahboobeh Nazari

Article in Press

Scanning electron microscope studies of pollen of herbaceous species of Amritsar, North-Western Punjab, India

Palynological studies in combination with conventional morphology have a significant role in solving problems relating to plant systematics. Scanning electron microscope (SEM) is a useful tool for palynological studies with increased accuracy and precision. The pollen size, shape and details of sculpturing of the exine are of taxonomic value. The main objective of the present study was to provide a detailed morphological...

Author(s):Harpreet Kaur and Avinash Nagpal

Article in Press

Characterisation of volatile compounds and flavour attributes of Lablab purpureus bean accessions

The Lablab bean (Lablab purpureus) has potential of being an outstanding resource for human food and animal feed in tropical agricultural systems. The bean is however grossly underutilized due anti-nutritional factors, which may affect its nutritive value and organoleptic properties. In this study, twenty-four (24) lablab bean accessions were assayed for the volatile compounds and sensory flavor characteristics to...

Author(s):Lablab purpureus, flavour, taste, volatile compounds.

Article in Press

Merzouga Sand Dunes Actinobacteria: antimicrobial potential and taxonomic diversity

Merzouga sand dunes were among the Moroccan extreme environments. Actinobacteria from this under investigated desert habitat were isolated and their antimicrobial activity was evaluated. One hundred sixty three Actinobacteria isolates were isolated from sand collected from several dunes and screened for their antibacterial and antifungal activities. To test the antimicrobial effect of isolates, four microorganisms known as...

Author(s):Lahcen Ouchari, Amal Boukeskasse, Brahim Bouizgarne and Yedir Ouhdouch

Article in Press

Preservative potentials of essential oils of three Nigerian spices in mixed fruit juice and their antioxidant capacity

The study was conducted to evaluate the preservative potentials of essential oils (EOs) of Piper guineense, Xylopica aethiopica and Tetrapleura tetraptera in mixed fruit juice and determine their antioxidant capacity. The preservative effect at varying concentrations was evaluated using S. cerevisiae, S. aureus and B. cereus as test isolates. The antioxidant properties were determined using 1,1- diphenyl-2-picrylhydrazyl...

Author(s):Ogueke, Chika C., Nnadi, Nkechi B., Owuamanam, Clifford I., Ojukwu, Moses, Nwachukwu, Ikenna N., Ibeabuchi, Chidi J and Bede, Evelyn N.

Article in Press

Development and Validation of a HPLC-DAD Method for the Quantification of Rutin and Isoquercetin in Morus nigra L. (Moraceae)

Morus nigra L. is widely used by the population of the São Francisco Valley for the treatment of several diseases such as hypertension, diabetes, arthritis, among others. The objective of this study was to develop and validate an analytical method that is capable of identifing and quantifing flavonoids in the ethanolic crude extract (Mn-EtOH) and its ethyl acetate phase (Mn-AcOEt) obtained from M. nigra leaves using HPLC....

Author(s):Pedrita Alves Sampaio,Grasielly Rocha Souza,Pedro Guilherme Sousa de Sá,Nathália Andrezza Carvalho de Souza, José Marcos Teixeira de Alencar Filho, Pedro José Rolim-Neto, Jackson Roberto Guedes da Silva Almeida, Larissa Araújo Rolim

Article in Press

Isolation and regeneration of protoplasts from leaf explant of Rhyncholaelia digbyana.

A protocol is presented for the isolation of protoplasts and regeneration of Rhyncholaelia digbyana. The protoplasts were isolated using hemicellulase enzymes at 1.5%, 2.25% and 3% (w/v), pectinase at 0.5% and 0.75% (w/v) and cellulase at 1% and 2% (w/v). Counting of the protoplasts was carried out with a Neubauer camera and an optical microscope at 40X, viability was determined with Evans blue dye at 0.025% (w/v). The...

Author(s):Lopez Puc Guadalupe

Article in Press

Simple and Rapid Detached Leaf Technique for Screening Common Beans (Phaseolus Vulgarise L.) In vitro Against Angular Leaf Spot (Pseudocercospora griseola) Disease

A rapid and reliable detached-leaf technique was developed for screening common bean (Phaseolus vulgaris) genotypes against angular leaf spot (ALS) disease caused by Pseudocercospora griseola. It is helpful to differentiate between susceptible and resistance common bean genotypes. Detached leaves of common bean tested genotypes were inoculated by immersing them into a spore suspension followed by placing into petri dishes...

Author(s): Tedla Yayis Rezene

Article in Press

Effect of sample extraction, preparation methods on HPLC quantification of plumbagin in in vivo and in vitro plant parts of Plumbago zeylanica L.

Plumbagin is an important therapeutic compound of Plumbago zeylanica L., the commercial demand of this compound warrants optimizing a suitable method to isolate plumbagin in large scale. The present study was undertaken to obtain the maximum recovery of plumbagin content by employing different extraction methods viz., ultra-assisted extraction (UAE), maceration extraction(ME), soxhlet extraction(SE), serial soxhlet...

Author(s):Krishna Muralidharan • Malaiyandi Jayanthi • Ramasamy Surendran• Muthusamy Balasubramanian • Shanmugam Girija

Article in Press

Anti-diabetic and some haematological effects of aqueous and ethanol leaf extract of Eriosema psoraleoides in alloxan-induced diabetic Wistar rats

This study investigates the anti-diabetic and some hematological effects of aqueous and ethanol leaf extracts of Eriosema psoraleoides in alloxan-induced diabetic Wistar rats. Forty-eight (48) albino mice were used for the lethal dose (LD50) study, while twenty-eight (28) Wistar rats were used for the diabetic and haematological study. The result of the phytochemical analysis shows some important phytochemicals that could...

Author(s):Nduka, Florence O., Ogugua, Victor N., Joshua, Packer E., Okpachi, Victor E., Gometi, Sandra A. and Nwigwe, Juliet O.

Article in Press

Co-expressing House Fly Cytochrome P450 6A1 and Human Cytochrome P450 Reductase in Escherichia coli to Bioconvert Insecticide Aldrin

Aldrin used in agricultural as well as industrial or home pest control is known to be a persistent organic pollutant, having chronic adverse effects on wildlife and humans. The degradation of aldrin has drawn a lot of attentions. In this study, we expressed house fly cytochrome P450 6A1 (CYP 6A1) or both house fly cytochrome P450 6A1 and human NADPH-P450 reductase (CPR) together in E.coli cells to study their activity on...

Author(s):Yating Wu, Yong Li, Xiaosheng Liang, Nan Jiang, Yunhua Wu

Article in Press

Performance of three epigeic earthworm species, Eisenia fetida, Eisenia andrie and Dendrobanae veneta, in managing Rose and Carnation wastes in flower industries

The horticulture sector in Ethiopia is being challenged by multifaceted hitches; among which managing excess wastes produced from the farms and minimizing the cost of inorganic fertilizers are the major ones. The performance of three epigeic earthworm species, Eisenia fetida, Eisenia andrie and Dendrobanae veneta, in managing flower (Rose and Carnation) wastes through vermicomposting was evaluated. The study was done using...

Author(s):SAID MOHAMMED HASSEN

Article in Press

IN VITRO REGENERATION OF NEOCARYA MACROPHYLLA (SABINE) PRANCE, WILD FRUIT OF NIGER.

Neocarya macrophylla is a spontaneous fruit species that constitutes the dominant population of Dallol Bosso in southwestern Niger. In order to test the in vitro regeneration ability of this species, micro-propagation was applied to micro-cuttings from young almond seedlings. The MS basal medium was enriched with several concentrations of NAA (0; 0.5 and 1 mg.L-1) and BAP (0; 0.5; 1; 1.5; 2; 3 mg.L-1). The first results...

Author(s): ABOUBACAR Kolafane

Article in Press

Clausenidin upregulated p53 and caused apoptosis in HT-29 tumor cell lines

Introduction: Clausenidin is a pyranocoumarin majorly found in medicinal herbs of the Rutaceae family used to treat cancer patients locally in Asia. The compound is presumed to have anti-cancer cell effect but its exact mechanism of action is still unknown. Aim: The study aimed to evaluate the effect of pure clausenidin on p53-mediated apoptosis as well as other cell death pathways in colon cancer (HT-29) cell lines....

Author(s):Peter M Waziriab, Rasedee Abdullahc, Rosita Roslia, Abdul Rahman Omard, Ahmad Bustamam Abdula, Nur Kartinee Kassime, Ibrahim Malamia, Imaobong C Ettia, Palanisamy Arulselvand and Ashwaq Shakir Al-Abboodia.

Article in Press

Evaluation of plant aqueous extracts as anti-diabetic agents on alloxan induced rabbits

In this study, effect of Barley (Hordeum vulgare), Black plum (Syzygium cumini) and Chinese tree leaves (Pistacia chinesis) aqueous extracts was investigated on serum glucose, cholesterol, urea levels and body weight in normal and alloxan-induced diabetic male rabbits. Rabbits were kept in fasting condition for 12 h with 150 mg/Kg dose of alloxan monohydrate was introduced. From 7th day of diabetes induction, blood serum...

Author(s):Hadia Akber Samoo ✝, Jagarwanti Maheshwari✝, Atta Muhammad Babar, Ather Hameed Sheikh ,Imrana Khushk, Muhammad Aqeel Bhutto, Jawaid Ahmad Khokhar, Abdul Sattar Qureshi

Article in Press

Protocol optimization of in vitro propagation of an endangered endemic Ethiopia medicinal plant, Echinops kebericho (Mesfin), using shoot tip

Echinops kebericho (Mesfin)isthe most important and critically endangered endemic Ethiopian medicinal plant. This plant is threatened due to over harvesting of its roots for medicinal purpose, and from seed viability and seed germination problem. The aim of this research was to build up an efficient in vitro propagation protocol for E. kebericho using shoot tip explants. Shoots excised from in vitro germinated seedlings...

Author(s):Muluken Enyew; Tileye Feyissa

Article in Press

Development of phage mediated quartz crystal microbalance (QCM) sensor for the detection of Escherichia coli, a food-borne pathogen

This study presents the development of phage mediated quartz crystal microbalance (QCM) sensor for the detection of Escherichia coli. Bacteriophage is very specific to host bacterial cell also known as parasite for bacteria. The Electrochemical Quartz Crystal Microbalance (EQCM) is a very sensitive device that measures a mass change per unit area by measuring the change in resonant frequency of a quartz crystal. A...

Author(s):Vipin Singh and Pranay Jain

Article in Press

Morphological diversity studies among sugarcane (Saccharum spp.) somaclones generated through callus culture

Callogenesis is one of the tools in sugarcane tissue culture for generating agronomically significant variation. Tissue culture derived variations are known as somaclonal variation. The study was carried out to investigate the effect of MS media supplemented with 1.0 mgL-1 of NAA (naphthalene acetic acid) and 2.0, 2.5, 3.0, 3.5 and 4.0 mgL-1 of 2,4-D (2,4-dichlorophenoxy acetic acid) on somaclonal variation in in vitro...

Author(s):Richard Wekesa, Justus M. Onguso, Bernard A. Nyende and Leonard S. Wamocho

Article in Press

Biodegradation of crude oil by Bacillus species isolated from different sources

The consequences of oil pollution to the environment and human health as a result of exploration activities, transportation and usage of petroleum compounds, has become a serious challenge to environmental scientists. This study evaluates the potential of using Bacillus subtilis in biodegradation of petroleum compounds. Ten Bacillus strains from non-petroleum contaminated sites were identified on the basis of microscopy...

Author(s):Mohammed Inuwa Ja’afaru, Chimezie Godwin Azuchukwuene and Aisha Sa’id Shittu

Article in Press

Analytical quantification of copper in frogs (Rana tigrina) found from various aquatic habitats

This study focused on the quantitative determination of Cu metal in various physical parts of frogs by using the Atomic Absorption Spectrometry technique). Cu was measured (2.72±0.77 µg/g) in kidney of Rana tigrina captured from industrial site and in R. tigrina captured from non-industrial site (28.10±27.02 µg/g). Similarly, 27.56±9.92 µg/g Cu was discovered in liver of R. tigrina captured from industrial site and...

Author(s):Muhammad Nasir, Tariq Mahmood Ansari, Ghulam Yasin, Aleem Ahmad Khan and Muhammad Shoaib

Article in Press

Marker-assisted pyramiding of Xa21 and Xa7 genes conferring resistance to bacterial leaf blight in indica cultivar Bacthom7

Bacterial leaf blight (BLB) of rice is one of the most destructive diseases affecting rice fields. Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of BLB. Two BLB resistance genes, Xa21 and Xa7, were transferred into the susceptible indica cultivar Bacthom7 (BT7) by using marker-assisted selection with markers pTA248 for Xa21 and ID7 for Xa7. Improved BT7 lines carrying the two resistance genes were inoculated with...

Author(s):Nguyen Thi Thu#, Vu Hong Quang#, Mai Van Tan#, Vu Đuc Lam, Nakano Toshitsugu, Nguyen Thi Hue, Nong Thi Hue, Nguyen Quoc Trung, Nguyen Viet Long, Nguyen Van Hoan and Vu Van Liet

Article in Press

Isolation and identification of bacterial endophytes from Crinum macowanii Baker

The widespread distribution of Crinum macowanii across the African continent has entrenched the plant’s medicinal usage in treating diverse diseases. While its phytochemistry is well established, its microbial symbionts and their utility have not been described. As such, five bacterial endophytes, viz. Staphylococcus species C2, Staphylococcus species C3, Bacillus species C4, Acinetobacter species C5 and Staphylococcus...

Author(s):Rebotiloe F. Morare, Eunice Ubomba-Jaswa, and Mahloro H. Serepa-Dlamini

Article in Press

Psychrotrophic proteolytic bacteria and their characterization from landfill soil under temperate climatic conditions of Kashmir Himalaya

During cold season in Kashmir valley, the decomposition of municipal solid waste slows down due to slowing down of microbial activity as a result, the waste gets piled up and its management becomes a greater challenge. Psychrotrophic proteolytic bacteria play an important role in the degradation of protenaceous materials of the waste stream under low temperatures that produces nitrogen sources for rapid functioning of...

Author(s):Basharat Hamid, Arshid Jehangir and Zahoor Ahmad Baba

Article in Press

Antioxidant activity of essential oil of three cultivars of Amomum subulatum and standardization of high performance thin layer chromatography (HPTLC) method for the estimation of 1,8-cineole

Essential oils of the fruits of three cultivars of Amomum subulatum (Family-Zingiberaceae), such as varlangy, seremna and sawney were isolated. Antioxidant potential was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) scavenging and FeCl3-reducing models and the presence of 1,8-Cineole was quantified using the developed high performance thin layer...

Author(s):Aftab Alam and Rita Singh Majumdar

Article in Press

Holistic plant regeneration protocol from callus cultures of Salvia moorcroftiana, a medicinal herb

Salvia moorcroftiana is a medicinal herb of the family Lamiaceae. The objective of this study was to develop an in vitro protocol for the rapid propagation of this species. Organogenesis by means of callus culture was carried out on Murashige and Skoog medium by the transfer of callus from an induction medium supplemented with α-naphtalenacetic acid (NAA, 1.0 mg/L) to regeneration medium containing 6-benzyladenin (BA, 1.0...

Author(s):Tour Jan, Ali Hazrat ,Rashid Azam, Arshad Ali Mohammad Nisar, Nasrullah Khan and Muhammad Asif Nawaz

Article in Press

Bioremediation potentials of Sunflower (Tithonia diversifolia) and pseudomonas species in soil contaminated with lead and zinc.

This study was conducted to assess the ability of sunflower (Tithonia diversifolia) inoculated with Pseudomonas species to remediate soils contaminated with heavy metals (lead and zinc). The contaminants were added to 20 kg soil in pots as lead nitrate (Pb (NO3)2) and zinc nitrate (Zn (NO3)2); 400 mg/l metal concentrations were added to 20 kg soil in pots. The experimental design was a randomized complete block design...

Author(s):Elizabeth Temitope Alori, Abiodun Joseph, Ojo Timothy Vincent Adebiyi, Ajibola Patrick Aluko and Chidera Onyekankeya

Article in Press

Genetic diversity among elite chickpea (Cicer arietinum L.) varieties of Ethiopia based on inter simple sequence repeat markers

The inter simple sequence repeat (ISSR) marker was used in this study for genetic fingerprinting and identification of released chickpea varieties and for the determination of the genetic relationships among these cultivars. A total of 19 released chickpea varieties were subjected to ISSR analysis with the objective of evaluating the genetic diversity among the cultivars. A total of 20 ISSR primers were initially screened...

Author(s):Zerihun Jida, Shiferaw Alemu and Destaw Mullualem

Article in Press

Antibacterial saponins from the leaves of Polyscias fulva (Araliaceae)

Saponins are a major family of secondary metabolites that occur in a wide range of plant species. Bioassay - guided fractionation of extract of the leaves of Polyscias fulva led to the isolation of three known saponins named, 3-O-α-L-arabinopyranosyl-hederagenin (1), 3-O-[α-L-rhamnopyranosyl(1-2)-α-L-arabinopyranosyl]-hederagenin (2) and...

Author(s):Winnie Cherotich Maritim, Isabel Nyokabi Wagara and Josphat Clement Matasyoh

Article in Press

Genetic variability, heritability and genetic advance for green pod yield and yield related traits in chili (Capsicum annum L.) accessions in Ethiopia

A field experiment was conducted to study the genetic variability, heritability and genetic advance for 12 quantitative characters in chili (Capsicum annum L.) accessions in Ethiopia, during the off-season period of 2012/2013 under flood irrigation at the Adet Agricultural Research Centre field. The experiment was conducted using Randomized Complete Block Design with three replications and a total of 25 accessions and four...

Author(s):Tadesse Tilahun, Yohannes Petros and Yigzaw Dessalegn

Article in Press

Effects of temperature and time on oil extraction from some Nigerian indigenous fresh water microalgae species

This study determined the effects of temperature and time on oil extraction from indigenous freshwater microalgae: Dictyosphaerium, Chlorella, Desmodesmus and Cosmarium species, cultured in 15 2-L column photobioreactors (PBR) (three per species). Growth and specific growth rates for the mixotrophic>autotrophic cultivation of the species were determined. Oil was extracted from the dried microalgae species at temperatures...

Author(s):Felix Uzochukwu Asoiro, Wilfred Ifeanyi Okonkwo, Clement Onyeaghala Akubuo, Nkechinyere O. Nweze and Cosmas Ngozichukwu Anyanwu

Article in Press

Atomic absorption spectroscopic evaluation of some heavy metal contents in tomato (Lycopersicon esculentum Mill) fruit and Ribu River water used for irrigation at Fodugora Farm, Weliso Town, Ethiopia

The levels of some heavy metals in mature fruits of tomato (Lycopersicon esculentum Mill) grown at Fodugora Farm, Weliso Town (Ethiopia) with Ribu River effluent water, used for its irrigation, have been determined using flame atomic absorption spectroscopic (FAAS) technique. Whereas, the levels of Fe, Zn and Pb in tomato fruits were: 34.05, 4.05, and 1.32 mg/L respectively, their concentrations in the river effluent water...

Author(s):Gera Techane, O. P. Yadav and LokeshYadav

Article in Press

In vivo evaluation of aggressiveness, pathogenicity and patulin accumulation by Penicillium expansum strain isolated from Algerian apples

Penicillium expansum is one of the most important postharvest pathogens of apple fruit worldwide. It causes blue mold decay, one of the important economical diseases of apple fruits and it is the main producer of patulin. The main objective of this study is to evaluate the aggressiveness and patulin production of an isolated strain from apples produced in the East of Algeria. The strain was isolated, screened for its...

Author(s):Hamida KROUMA, Radia CHERFIA, Atef JAOUANI, Amor MOSBAH, Soukaina ABDELWAHAD, Mounira KARA ALI and Noreddine KACEM CHAOUCHE

Article in Press

Molecular epidemiological studies on Trypanosoma evansi type A and type B in camels (Camelus dromedaries) from five different regions of Saudi Arabia using the ITS1 rDNA and RoTat 1.2 VSG gene

Trypanosoma evansi is the most widespread of the pathogenic salivarian trypanosomes and cause a serious disease called surra that affects domestic animals such as camels and horses in tropical and subtropical countries and often leads to reduced productivity and economic losses. Therefore, the objectives of the present study were to determine the prevalence rates of trypanosomiasis using polymerase chain reaction (PCR)...

Author(s):Abdullah D. Alanazi

Article in Press

Comparative survey of three processes used for the extraction of total phenol content and total flavonoid content of Anacardium occidentale L. and the assessment of its antioxidant activity

Three processes were used for the extraction of total phenol and total flavonoid contents of Anacardium occidentale L. and to determine its antioxidant activity. Whatever the extraction process was, the total flavonoid and total phenol contents were of the same order. With the microwave-assisted extraction (MAE), 666.07 mg gallic acid equivalent (GAE)/100 g dry matter (DM) of total phenol content was obtained from the stem...

Author(s):MADJITOLOUM BETOLOUM Salomon

Article in Press

Genetic diversity assessment of yams (Dioscorea spp.) from Ethiopia using inter simple sequence repeat (ISSR) markers

The genetic diversity and relationships of 70 accessions of yam belonging to Dioscorea cayenensis/Dioscorea rotundata complex (55), Dioscorea bulbifera (13) and Dioscorea alata (2, as a reference) were assessed using six inter simple sequence repeat (ISSR) primers. DNA was extracted from a bulk of two plants per accession using a modified cetyl trimethyl ammonium bromide (CTAB) method. Six ISSR primers amplified 77...

Author(s):Kedra Mohammed Ousmael

Article in Press

Study of secondary metabolites produced by Aspergillus flavus and evaluation of the antibacterial and antifungal activity

The objective of this study was the analysis of the secondary metabolite products and evaluation of antibacterial activity of Aspergillus flavus. Bioactives are chemical compounds often referred to as secondary metabolites. Thirty one bioactive compounds were identified in the methanolic extract of A. flavus. The identification of bioactive chemical compounds is based on the peak area, retention time, molecular...

Author(s):Ghaidaa J. Mohammed, Imad H. Hameed and Sabreen A. Kamal

Article in Press

Opaque-2 Gene and Beta Carotene Content in 20 Yellow-, Red and Purple-Kernelled Maize (Zea Mays L.) Accessions in Ghana.

Twenty yellow-, red- and purple-kernel maize accessions were collected from three geographic regions in Ghana for the study. The objective was to determine the presence of opaque -2 gene and -carotene content of the grains as a means of assessing nutritional quality. Variability in -carotene content was determined by HPLC while presence of opaque 2-gene was determined by a light box. Significant variations were found in...

Author(s):GILBERT ANSAH and KOJO AHIAKPA

Article in Press

Microbial machinery based synthesis of nanoparticulate hydroxyapatite using Serratia marcescens and its characterization for human bone implant

Hydroxyapatite (HAp), chemically and compositionally similar to the material presents in bone and has a unique biocompatibility which makes it an attractive material for bone implant. We aimed at studying the physical and chemical properties of HAp synthesized by Serratia sp. The total of 58 strains was isolated from 354 samples obtained from clinical and environmental sources like urine, sputum, soil, water and plant...

Author(s):Rathinasamy Subashkumar, MIDHUSHA JOHNY and Nandhagopal Gayathri

Article in Press

Fatty acids profiling of the seed oils of some varieties of peanut (A.hypogaea L.) towards the development by GC-MS analysis.

Twelve fatty acids were identified during maturity in the wild Tunisian and cultivar peanut kernels. These fatty acids include C16:0 (palmitic acid), C18:0 (stearic acid), C18:1 (oleic acid), C18:2 (linoleic acid), C19:0 (nonadecanoic acid), C20:1 (gadoleic acid), C20:0 (arachidic acid), C22:1 (erucic acid), C22:0 (behenic acid), C23:0 (tricosanoic acid), C24:0 (linoceric acid), and C26:0 (cerotic acid). Three fatty acids...

Author(s):Aicha. O. CHERIF, Mhamed BEN MESSAOUDA, Habib KALLEL, Claude PEPE

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