Journal of
Developmental Biology and Tissue Engineering

  • Abbreviation: J. Dev. Biol. Tissue Eng.
  • Language: English
  • ISSN: 2141-2251
  • DOI: 10.5897/JDBTE
  • Start Year: 2009

JDBTE Articles

April 2015

Micropropagation of wild fennel (Foeniculum vulgare var. vulgare) via organogenesis and somatic embryogenesis

Wild fennel (Foeniculum vulgare var. Vulgare) is a perennial aromatic herb. It is native to the Mediterranean region and currently it iscultivated as an annual or perennial herb worldwide. Dried ripe fruits are commonly used plants part for obtaining essential oil. Fruits oil of wild fennel contains many volatile oils, such as α-pinene, phellandrene, p-cymene, fenchone, estragole, anethole and anisaldehyde....

Author(s): Rehab H. Abd-Allah, Ehsan M. Abo Zeid, Mahmoud M. Zakaria and Samih I. Eldahmy

July 2014

A study on the formation and development of Panax bipinnatifidus Seem. adventitious root

This study aimed to generate adventitious roots from root and stem-derived callus of Panax bipinnatifidus Seem. Callus formation was observed best (83.3%) in MS media supplemented with 3.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg/L indolebutyric acid (IBA). Callus generated adventitious roots in MS media supplemented with 2.0 mg/L IBA at high frequency (66.7%). The optimal culture condition for the...

Author(s): Bui DinhThach, Lenguyen Tu Linh, Nguyen ThiThuy Van, Trinh Thi Ben, Mai Truong and Nguyen Huu Ho

May 2013

Morphology and histology of the alimentary tract of adult palm weevil, Rhynchophorus phoenicis Fabricius (Coleoptera: Curculionidae)

Palm weevil (Rhynchophorus phoenicis) is a notorious pest of oil palm trees, worldwide. Investigations were conducted on the alimentary tract of the adult insect. Results of the morphology and histology of the alimentary tract revealed that the alimentary tract consisted of a foregut, which was made up of a buccal cavity, oesophagus, crop and proventriculus. The crop big or small and the proventriculus had bristle...

Author(s): Omotoso Olumuyiwa Temitope

May 2013

Markers are shared between adipogenic and osteogenic differentiated mesenchymal stem cells

The stem cell differentiation paradigm is based on the progression of cells through generations of daughter cells that eventually become restricted and committed to one lineage resulting in fully differentiated cells. Herein, we report on the differentiation of adult human mesenchymal stem cells (hMSCs) towards adipogenic and osteogenic lineages using established protocols. Lineage specific genes were evaluated by...

Author(s): Melanie Köllmer, Jason S. Buhrman, Yu Zhang and Richard A. Gemeinhart,

April 2013

Future of dentistry, nanodentistry, ozone therapy and tissue engineering

Future of dentistry has become one of the most unique resources and providers of this oral systemic approach. In determining the patient's unique needs, it is becoming increasingly important for the dentist to work with other cutting-edge, integrative practitioners to fully assess the individual requirements of the patient before, during, and after dental care. Witnessing the beginning of truly groundbreaking...

Author(s): Maryam Moezizadeh

April 2013

3-D osteoblast culture for biomaterials testing

Micromass cell cultures, osteomicrospheres, were formed by aggregation of primary osteoblasts. Cell differentiation during sphere formation and the integrity of the osteomicrospheres was evaluated by analyzing the immunohistochemical expression of osteonectin, osteocalcin, and collagen type I. Transmission electron microscopy facilitated the proof of the tissue-like microstructure inside the osteomicrospheres and the...

Author(s): Jörg Neunzehn, Sascha Heinemann and Hans Peter Wiesmann

May 2012

Round-bottomed Honeycomb Microwells: Embryoid body shape correlates with stem cell fate

The differentiation of embryonic stem cells (ESC) into tissue-specific cells utilizes either monolayer cultures or three-dimensional cell aggregates called embryoid bodies (EB). However, the generation of a large number of EB of controlled sizes can be challenging and labor intensive. Our laboratories have developed a simple, robust, ultra-rapid, and inexpensive design of Honeycomb Microwells for generation of EB. Here,...

Author(s): Silin Sa, Diep T. Nguyen, Jonathan D. Pegan, Michelle Khine and Kara E. McCloskey, Graduate Group in 

May 2012

Study of cephalometric parameters among 4 to 11 years old Persian girls resident in Iran

Anthropometry is applied in medical profession such as maxillofacial surgery, growth and development studies, plastic surgery, bioengineering and non-medical branches such as like shoe-making and eye-glasses industries. The aim of the present study was to determine facial and cranial parameters among 4 to 11 years old Mashhadian young girls in order to assess growth pattern in this age range. 564 Persian girls aged...

Author(s): Esmaeilzadeh Mahdi, Kazemzadeh Fariba and Adibfar Nader

April 2012

Mimicry of a natural, living intra-epidermal micro pattern used in guided tissue regeneration of the human epidermis

The methodology for the isolation of the living, intra-epidermal micro pattern using a tissue culture method is described. The disassembly of the stripped off epidermis (after Dispase digestion at 37°C) into epidermal brown rosettes (their morphology under a phase contrast microscope) constitute intra-epidermal micro patterns. The stepwise reasoning in the recognition of epidermal basal layer rosettes and the...

Author(s): Denis E. Solomon

April 2012

An investigation on cephalometric parameters in Iranian population

Anthropometry is the biological science of human body measurement. Anthropometry is applied in medical profession such as maxillofacial surgery, growth and development studies, plastic surgery, bioengineering and non-medical branches such as like shoe-making and eye-glasses industries. The aim of this study was to determine Iranian cephalometric parameters and cranial and facial anthropometric ratios. This cross...

Author(s): Esmaeilzadeh Mahdi, Nejadali Abolfazl, Kazemzadeh Fariba and Borhani Mohammad

November 2011

Mesenchymal stem cells and acquisition of a bone phenotype: An ion channel overview

Bone remodeling is a physiological process determined by the sequential and coordinated interaction of osteocytes, osteoclasts, osteoblasts and angiogenesis. During bone repair, osteoblastic cells, originated from mesenchymal stem cells (MSCs), are highly regulated to proliferate and to produce an osteogenic matrix, thus forming a new bone. MSCs are multipotential and undifferentiated cells that are present in the adult...

Author(s): Priscilla C. Aveline, Julie I. Bourseguin and Gaël Y. Rochefort

November 2011

Achievement of balanced oncogenes and tumor-suppressor genes activity in normal and malignant cells in vitro and in vivo

The main goal is connected with providing, on the one hand, of active tumor-suppressor genes for prevention of eventual malignant transformations, and, on the other hand, of functionally active oncogenes for prevention of early aging and death, both in vitro and in vivo. Modulation of an adequate immune control was also necessary, and in this way any eventual unwished side effects from the genetic...

Author(s): Iskra Ventseslavova Sainova, Ilina Vavrek, Velichka Pavlova, Teodora Daneva, Ivan Iliev, Lilija Yossifova, Elena Gardeva and Elena Nikolova

  • Article Number: 5343BD310561

August 2011

Micropropagation of Maerua oblongifolia: A rare ornamental from semi arid regions of Rajasthan, India

A method for in vitro regeneration of Maerua oblongifolia (Capparaceae) from nodal shoot explants is outlined. Percent shoot response with multiplication rate (21.1± 2.33) shoots per explant (30 mm length) was achieved when cultured on semisolid Murashige and Skoog (MS) medium containing 3% sucrose and supplemented with 2.0 mgl-1 of (Benzylaminopurine) BAP + additives (25.0 mgl-1adenine...

Author(s): Mahender Singh Rathore and Narpat Singh Shekhawat

  • Article Number: 4EDE05610537

August 2011

Ultrasonic analyses algorithm on an ex vivo produced oral mucosal equivalent

This study examines the use of high-resolution ultrasound with an analyses algorithm to accurately monitor development of an ex vivo produced oral mucosal equivalent (EVPOME). We used ultrasonic profilometry to examine EVPOME development as seeded cells on its surface proliferate. As these engineered structures develop, seeded cells stratify from their differentiation and produce a keratinized protective upper...

Author(s): Frank Winterroth, Junho Lee, Shiuhyang Kuo, J. Brian Fowlkes, Stephen E. Feinberg, Scott J. Hollister and Kyle W. Hollman,

  • Article Number: 0E74CBD10546

July 2011

Challenges of bioengineering and endodontics

Stem cell research and scaffolding are now the buzz words in basic science pulp researches. The endodontic community needs to enhance its clinical understanding of the vital pulp and dentin, and embrace new treatment modalities. Several regenerative techniques have been described, while therapies involving stem cells, growth factors and scaffolds have been proposed. Each technique has its own advantages and...

Author(s): Anil Dhingra, Viresh Chopra and Shalya Raj

  • Article Number: DF7BE6010527

July 2011

Ultrastructural changes in the tight intercellular junctions of the endoneurial blood vessels following sural nerve crush injury in rats

The objective of this study was to investigate the ultrastructural changes in the tight intercellular junctions of the endoneurial blood vessels after nerve injury. Twenty-one adult male Sprague-Dawley rats were used in the study (3 animals as a control group and left sural nerves of 18 animals were subjected to the crush). At intervals of 1, 2, 3, 4, 5 and 6 weeks after crush, 3 rats /sitting were killed and the fixed...

Author(s): Wagih G. Elbarrany and Faris M. Altaf      

  • Article Number: 753932240379

June 2011

Healthcare workers uniforms (HCWU)

There is a major concern for healthcare workers (HCW) regarding transmission of bacteria to and from their patients. Because of this potential contamination, protection is a major issue. Healthcare workers uniforms (HCWU) are often used as barriers to help eliminate or reduce the risk of infection for both the doctor and the patient. Without sufficient barriers, harmful bacteria can reach and penetrate the skin of...

Author(s): Salah A. Latief Mohammed

  • Article Number: 80BBC9610461

June 2011

Fibrin sealant as scaffold can be a suitable substitute to autograft in short peripheral nerve defect in rats

There is considerable evidence that peripheral nerves have the potential to regenerate in an appropriate microenvironment. In this study, the process of nerve regeneration through a fibrin scaffold was examined. 45 male Wistar rats were randomly divided into one control, Autograft (Auto) and two experimental groups, Epineurum (Epi) and Fibrin scaffold (Fib). Right sciatic nerve was exposed of which 5 mm was cut. The...

Author(s): Z. Jamalpoor, M. Ebrahimi, N. Amirizadeh, K. Mansoori, A. Asgari and M. R. Nourani,

  • Article Number: B758E1010466

June 2011

Healthcare workers uniforms (HCWU)

There is a major concern for healthcare workers (HCW) regarding transmission of bacteria to and from their patients. Because of this potential contamination, protection is a major issue. Healthcare workers uniforms (HCWU) are often used as barriers to help eliminate or reduce the risk of infection for both the doctor and the patient. Without sufficient barriers, harmful bacteria can reach and penetrate the skin of...

Author(s): Salah A. Latief Mohammed

  • Article Number: D423FCE10542

June 2011

Fibrin sealant as scaffold can be a suitable substitute to autograft in short peripheral nerve defect in rats

There is considerable evidence that peripheral nerves have the potential to regenerate in an appropriate microenvironment. In this study, the process of nerve regeneration through a fibrin scaffold was examined. 45 male Wistar rats were randomly divided into one control, Autograft (Auto) and two experimental groups, Epineurum (Epi) and Fibrin scaffold (Fib). Right sciatic nerve was exposed of which 5 mm was cut. The...

Author(s): Z. Jamalpoor, M. Ebrahimi, N. Amirizadeh, K. Mansoori, A. Asgari and M. R. Nourani,

  • Article Number: A9E983610551

May 2011

Influence of osteogenic supplements on the osteoclastogenesis of human monocytes

A basic requirement for bone remodeling is the communication between bone forming osteoblasts and bone resorbing osteoclasts – the so-called cross talk. Corresponding in vitro co-culture models might be a valuable technique in order to investigate the influence of novel biomaterials on the cross talk. Assuming that both cell types are derived by precursor cells, the role of the common osteogenic...

Author(s): Christiane Heinemann, Sascha Heinemann, Corina Vater, Hartmut Worch and Thomas Hanke

  • Article Number: F8E5D6C10443

May 2011

In vitro proliferation of shoot regeneration from embryo of Cajanus cajan L. (var.LGG-29)

An efficient and direct shoot bud differentiation and multiple shoot induction from embryo explants of pigeon pea (Cajanus cajan L.) has been achieved. The frequency of shoot bud regeneration was influenced by the type of explant, genotype and concentrations of cytokinin. Explant embryo were cultured on Murashine and Skoog (MS) medium augmented with different concentrations of Benzyl amino purine (BAP). Among the...

Author(s): M. Guru Prasad, T. N. V. K. V. Prasad and P. Sudhakar

  • Article Number: 33E151C10450

May 2011

Influence of osteogenic supplements on the osteoclastogenesis of human monocytes

A basic requirement for bone remodeling is the communication between bone forming osteoblasts and bone resorbing osteoclasts – the so-called cross talk. Corresponding in vitro co-culture models might be a valuable technique in order to investigate the influence of novel biomaterials on the cross talk. Assuming that both cell types are derived by precursor cells, the role of the common osteogenic supplements...

Author(s): Christiane Heinemann, Sascha Heinemann, Corina Vater, Hartmut Worch and Thomas Hanke

  • Article Number: 7F0E8DE10506

May 2011

In vitro proliferation of shoot regeneration from embryo of Cajanus cajan L. (var.LGG-29)

An efficient and direct shoot bud differentiation and multiple shoot induction from embryo explants of pigeon pea (Cajanus cajan L.) has been achieved. The frequency of shoot bud regeneration was influenced by the type of explant, genotype and concentrations of cytokinin. Explant embryo were cultured on Murashine and Skoog (MS) medium augmented with different concentrations of Benzyl amino purine (BAP). Among the...

Author(s): M. Guru Prasad, T. N. V. K. V. Prasad and P. Sudhakar

  • Article Number: B63890210520

April 2011

Use of 3-dimensional environments to engineer osseous-like tissue

We have previously reported enhanced aggregate formation and accelerated mineralization of human mesenchymal preosteoblastic cells (HEPM) when cultured without scaffolds in 3D-rotary wall vessel chambers. From those studies we hypothesized that, rotary wall vessel environments could be used to engineer osseous tissue. Thus, HEPM preosteoblastic cells were cultured in rotary wall vessels for up to 7 days. Progression of...

Author(s): G. B. Schneider, J. K. Boehrs, J. V. Hoopes and D. A. Seabold

  • Article Number: 38B060A10412

April 2011

Recent studies on the pullout strength behavior of spinal fixation

Author(s): Mohammed Shahril Azwan and Inzarulfaisham Abd Rahim

  • Article Number: FEC2DBC10421

April 2011

Use of 3-dimensional environments to engineer osseous-like tissue

We have previously reported enhanced aggregate formation and accelerated mineralization of human mesenchymal preosteoblastic cells (HEPM) when cultured without scaffolds in 3D-rotary wall vessel chambers. From those studies we hypothesized that, rotary wall vessel environments could be used to engineer osseous tissue. Thus, HEPM preosteoblastic cells were cultured in rotary wall vessels for up to 7 days. Progression of...

Author(s): G. B. Schneider, J. K. Boehrs, J. V. Hoopes and D. A. Seabold

  • Article Number: 356DD5910472

April 2011

Recent studies on the pullout strength behavior of spinal fixation

Spinal fixations with pedicle screws are widely used nowadays in order to provide spine stability and correcting spinal deformity. The pullout strength of pedicle screws can be evaluated by means of pullout strength testing. This paper reviews recent experimental and finite element analysis evidence concerning the factors that affecting the pullout strength of pedicle screws in various bone materials. Cadaveric bones...

Author(s): Mohammed Shahril Azwan and Inzarulfaisham Abd Rahim

  • Article Number: FB5985210484

March 2011

Nucleofection an efficient non-viral transfection technique forIFN-t-EGFP gene expression study in Sahiwal cattle fibroblast cells

Viral based techniques were considered to be the most efficient systems to deliver DNA into fibroblast cells as they show high transgene expression in many cellular models. Viral approaches are complicated by immune response, intracellular trafficking potential mutations and genetic alterations due to integration. The nucleofector TM technology is electroporation based gene transfer technique which has been proved to be...

Author(s): Anand Laxmi N., Gunjan G. and Prateesh M.

  • Article Number: 8CE855010393

March 2011

Time trends, and survival of patients with oral and pharyngeal malignancies

An accurate assessment of oral and pharyngeal malignancies in cancer treatment trends, and survival of the disease was missing in Egypt. Accordingly, all new cases treated in Alexandria and El Behira governorates during the last decade were studied retrospectively. Data were collected through all accessible archives using a special data collection sheet. The total populations of different governorates were obtained from...

Author(s): Waguih Mohamed Abouzeid, Samiha Ahmed Mokhtar, Nehad Hassan Mahdy, Mohamed Sherif Ahmed and Fayek Salah El Kwsky

  • Article Number: B38B42810403

March 2011

Nucleofection an efficient non-viral transfection technique forIFN-t-EGFP gene expression study in Sahiwal cattle fibroblast cells

Viral based techniques were considered to be the most efficient systems to deliver DNA into fibroblast cells as they show high transgene expression in many cellular models. Viral approaches are complicated by immune response, intracellular trafficking potential mutations and genetic alterations due to integration. The nucleofector TM technology is electroporation based gene transfer technique which has been proved to be...

Author(s): Anand Laxmi N., Gunjan G. and Prateesh M.

  • Article Number: D079FC210431

March 2011

Time trends, and survival of patients with oral and pharyngeal malignancies

An accurate assessment of oral and pharyngeal malignancies in cancer treatment trends, and survival of the disease was missing in Egypt. Accordingly, all new cases treated in Alexandria and El Behira governorates during the last decade were studied retrospectively. Data were collected through all accessible archives using a special data collection sheet. The total populations of different governorates were obtained from...

Author(s): Waguih Mohamed Abouzeid, Samiha Ahmed Mokhtar, Nehad Hassan Mahdy, Mohamed Sherif Ahmed and Fayek Salah El Kwsky

  • Article Number: 9D3C23610446

February 2011

Synthesis, spectroscopy and biological studies of Nickel (II) complexes with tetradentate Schiff bases having N2O2 donor group

Complexes of nickel(II) of N,N’-disalicylidene-3,4-diaminotoluene(H2L1), N,N’-bis(3,5-di-tert-butylsalicylidene)-1,3-diaminopropane(H2L2),tetrathiafulvalene-N,N’-phenylene bis (salicylideneimine)(H2L3), o-hydroxybenzaldehyde, o-hydroxyacetophenone ethylene diamine (H2L4) and 1-phenylbutane-1,3-dionemono-S-methylisothio- semicarbazone with 5-phenylazo- o-hydroxybenzaldehyde (H2L5) have been synthesized...

Author(s): Anant Prakash, Mukesh Pal Gangwar and K. K. Singh

  • Article Number: DB3FE0E10353

February 2011

Optimization of medium conditions for efficient plant regeneration from embryo of cotton (var.narishima)

An efficient and rapid regeneration protocol was developed using embryo from germinating seedlings of cultivar of cotton (var.narishima) as an explant. A vertical slit given in the apex of embryo shoot regeneration was successful on murashige and skoog medium with 2 mg/L benzyl amino purine (BAP). The shoots were sub cultured in every 15 days in regeneration medium and later they were transferred to rooting medium (1...

Author(s): M. Guru Prasad, P. Sudhakar and T. N. V. K. V. Prasad

  • Article Number: 576A27710379

January 2011

Exogenous NGF favors initiation of lizard tail regeneration while EGF and TGF-β truncate regenerative growth and commit to precocious muscle and cartilage differentiation

Since growth factors exert control over various cellular events during development and as information in this regard on regenerative growth are scant, the present study has evaluated the effect of exogenous in loco administration of EGF, TGF-β and NGF during the first few days post-autotomy on the course of tail regeneration in Hemidactylus flaviviridis. Further evaluated is the efficacy of NGF to rectify the...

Author(s): Anju Kurup and A.V. Ramachandran

  • Article Number: 1AAD87410314

January 2011

Gastrimargus musicus muscle tissue culture

Cells from legs of Gastrimargus musicus, a grey brown grasshopper were grown in simple media. The medium was tested for growth of muscle cells and with supplements. It was found that the medium containing calcium carbonate had round cells growing close to each other in clumps. The medium containing creatine had cells growing which were elongated and branched and had all the features of fully developed muscle tissue. The...

Author(s): Shukla P., Singh A. and Gawri S.

  • Article Number: EDC3A1E10328

December 2010

Regeneration of germlings and seedlings development from cauline leaves of Sargassum thunbergii

  A method of producing artificial Sargassum thunbergii seedlings is urgently desired to meet the increasing demand of raw materials used for aquaculture and for the sake of environmental protection. This is the first report of attempt to study the vegetative propagation of this species using cauline leaves under laboratory conditions. On average, 45.75% of the excised leaves survived and one leaf could...

Author(s):   Feng Li, Shoutuan Yu, Yuze Mao and Naihao Ye

  • Article Number: 65283B210384

December 2010

Genistein, the phytoestrogen induces heart-and-soul (has) phenotypes in zebrafish embryo

Zebrafish (Danio rerio) embryos treated with a small plant molecule, genistein generated a mimic of zebrafish VEGF-A loss of function model. These embryos develop with an enlarged pericardium and major blood vessel deficiencies and their morphological assessment after 48 h post fertilization indicates a nearly complete absence of both axial and intersegmental vasculature with no or reduced numbers of circulating red...

Author(s): Antony Bakkiyanathan, Asha Mary Joseph, Loganathan Tharani and Raghunathan Malathi

  • Article Number: 555E6F610399

June 2010

Regeneration of germlings and seedlings development from cauline leaves of Sargassum thunbergii

A method of producing artificial Sargassum thunbergii seedlings is urgently desired to meet the increasing demand of raw materials used for aquaculture and for the sake of environmental protection. This is the first report of attempt to study the vegetative propagation of this species using cauline leaves under laboratory conditions. On average, 45.75% of the excised leaves survived and one leaf could produce several...

Author(s): Feng Li, Shoutuan Yu, Yuze Mao and Naihao Ye    

  • Article Number: 7BEF7F010343

June 2010

Synthesis, characterization and biological activity of some novel azetidinones

A series of seven novel azetedinones 4a-g have been synthesized by cyclocondensation of various Schiff bases of phenothiazine with chloroacetyl chloride in presence of triethylamine. Various Schiff bases of phenothiazine were synthesized by condensation of 2-hydrazinyl-1-(10H-phenothiazin-10-yl)ethanone 2 with various aryl aldehydes. Compound 2 was synthesized by reacting 2-chloro-1-(10H-phenothiazin-10-yl) ethanone 1...

Author(s): A. Rajasekaran, M. Periasamy and S. Venkatesan

  • Article Number: 5D3660610349

November 2009

Antibacterial activity of crude seed extracts of Buchholzia coriacea E. on some pathogenic bacteria

The antibacterial efficacy of hot water and methanol extracts of dried seeds of Buchholzia coriacea against Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Bacillus cereus, and Vibrio cholerae were determined using the Agar-gel diffusion method. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and phytochemistry of the extracts were also evaluated. Results obtained...

Author(s): T. I. Mbata, C. M. Duru and H. A. Onwumelu

  • Article Number: 839218C10252

November 2009

Photosynthetic properties of the protoplasts from Bryopsis hypnoides Lamouroux. during the early regeneration process

The scanning electronic microphotograph (SEM), photosynthetic oxygen evolution rate and excitation spectra at liquid nitrogen temperature (77 K) of the protoplasts from Bryopsis hypnoides were determined at the early regeneration period (12 h). SEM revealed an existence of the tight aggregation; cell membrane and wall were about 10 min, 6 and 12 h respectively, after the culture. Oxygen evolution rate decreased slowly...

Author(s): Nai Hao Ye, Hong Xia Wang, Zheng Quan Gao and Guangce Wang

  • Article Number: CDE1D6910290

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