Journal of
Biophysics and Structural Biology

  • Abbreviation: J. Biophys. Struct. Biol.
  • Language: English
  • ISSN: 2141-2200
  • DOI: 10.5897/JBSB
  • Start Year: 2009

JBSB Articles

September 2017

Extrapolating human toxicity data with target organ dose (TOD-Wb) model: A meso-scale allometric analytical approach

An allometric target organ dose model (TOD-Wb) with variable scaling exponent, b = br + bo(bm) - bi, is proposed for the extrapolation of animal toxicity to human. The exponent b comprises four constituent parameters representing the intake mode to entry organ (bi), route of transportation to target organ (br), mass of target organ (bo), and rate of metabolism at target organ (bm). This expression enables the a priori...

Author(s): Swee-Cheng FOO, Ivan Siang-Meng SIN, Hui-Fang ANG and Rachel Qian-Yun CHE

February 2014

Spectroscopic approach of the interaction study of ceftriaxone and human serum albumin

Under physiological conditions, interaction between ceftriaxone and human serum albumin was investigated by using fluorescence spectroscopy and ultra violet (UV) absorption spectrum. From spectral analysis, ceftriaxone showed a strong ability to quench the intrinsic fluorescence of human serum albumin (HSA) through a static quenching procedure. The binding constant (k) is estimated as K=1.02× 103 M-1 at 298 K....

Author(s): Abu Teir M. M., Ghithan J., Abu-Taha M. I., Darwish S. M. and Abu-hadid M. M.

July 2013

Epigenetic regulation of PGC1 α in human type 2 diabetes

Type 2 diabetes mellitus (T2DM) is the most common metabolic disease in theworld, reaching epidemic proportions. PPARGC1A mRNA expression is reduced in islets from patients with diabetes 2 and it is influenced by both genetic and epigenetic factor. The epigenetic modification, results as a two fold increase in DNA methylation of the PGC1A promoter of diabetes. This two fold increase in DNA methylation is due to the DNA...

Author(s): Y. Dhanusha Yesudhas

July 2013

Changes in airway resistance with cumulative numbers of cigarettes smoked

This work was performed with smokers of Virginia tobacco cigarettes without taking into consideration brand or type of cigarettes. An experimental study was conducted for the variation of cigarettes number and the increments in airway resistance for both normal and respiratory disordered subjects using body plethysmograph for testing individuals. Seventeen (17) nonsmokers and ten smokers were studied. The investigation...

Author(s): Almaasfeh Sultan

March 2012

The use of a continuity equation of fluid mechanics to reduce the abnormality of the cardiovascular system: A control mechanics of the human heart

The paper is aimed at presenting the differential equations for the cardiovascular system with the help of continuity equation of fluid mechanics to reduce the abnormality of the rate of blood flow and variation of blood volume in different parts of the system. The equations are used to explain the Frank-Starling mechanism, which plays an important role in the maintenance of the stability of the distribution of blood in...

Author(s): L. S. Taura, I. B. Ishiyaku and A. H. Kawo

March 2012

Using electrical impedance tomography in following up skin conductivity change for different sonophoresis conditions

Sonophoresis is the using of ultrasound waves to increase the entrancement of genes and drugs surpassing the skin barrier. Many mechanisms have been described to illustrate the sonophoresis mode of action; some describe changes in skin resistance during sonophoresis. Electrical impedance tomography (EIT) technique uses voltage measurement through a group of electrodes and reconstructs the data to a conductivity picture....

Author(s): Mamdouh M. Shawki and Abdel-Rahman M. Hereba

November 2011

pH uniquely modulates protein arginine methylation

Protein arginine methyltransferases (PRMTs) function in the alkaline milieu of the nucleus and at neutral pH of the cytosol. Accordingly, several PRMTs are broadly active over a range of pHs. We investigated the effect altering pH had on protein arginine methylation using a variety of defined substrates, recombinant PRMTs and cell extracts.We demonstrate that pH-induced alterations in the extent of methylation and the...

Author(s): Wen Xie, George Merz and Robert B. Denman

November 2011

Crystal structure of the allosteric-defective chaperonin GroELE434K mutant

The chaperonin GroEL adopts a double-ring structure with various modes of allosteric communication. The simultaneous positive intra-ring and negative inter-ring cooperativities allow alternating functionality of the folding cavities in both protein rings. Mutation of glutamic acid 434 (located at the ring interface), to lysine alters the negative inter-ring cooperativity. The crystal structure of the mutant chaperonin...

Author(s): Aintzane Cabo-Bilbao, Ariel E. Mechaly, Jon Agirre, Silvia Spinelli, Begoña Sot, Arturo Muga and Diego M.A. Guérin

September 2011

Effect of the tannoid enriched fraction of Emblica officinalis on α-crystallin chaperone activity under hyperglycemic conditions in lens organ culture

Chaperone-like activity (CLA) of α-crystallin is known to be compromised in diabetic conditions and associated with cataract formation. Protecting α-crystallin CLA may help in delaying and/or preventing cataracts. In this study, we employed a lens organ culture model to study the effect of hyperglycemia on the CLA of α-crystallin and investigated the protective effect the tannoids...

Author(s): P. Anil Kumar, P. Yadagiri Reddy, P. Suryanarayana and G. Bhanuprakash Reddy

  • Article Number: 85D4DDB10897

September 2011

Modeling and proposed mechanism of two radical scavengers through docking to curtail the action of ribonucleotide reductase

Ribonucleotide reductase (RR) is a ubiquitous cytosolic enzyme required for DNA synthesis and repair in all living cells. Therefore, the crucial role of this enzyme in cell division makes it a potential target for designing drugs that inhibit cell growth for cancer therapy. An increased interest in RR as a target for cancer therapy has been documented since the discovery that human RR is regulated by p53 enzyme and that...

Author(s): Sampath Natarajan and Rita Mathews

  • Article Number: 1A5A0C310908

April 2011

The salicylic acid effect on the tomato (Lycopersicum esculentum Mill.) sugar, protein and proline contents under salinity stress (NaCl)

Plants growth is impressed by biotic and abiotic stress inversely. There are many reports about proteins change level in salinity stress. Leaves fill up more soluble sugar of glucose, fructose and proline with treatment of salicylic acid. In this research, tomato seeds planted in pots containing perlite were put in a growth chamber under controlled conditions of 27 ± 2 and 23 ± 2°C temperature, 16...

Author(s): Shahba Zahra, Baghizadeh Amin, Vakili Seid Mohamad Ali, Yazdanpanah Ali and Yosefi Mehdi

  • Article Number: 39E13B910812

January 2011

EGFP-FMRP forms proto-stress granules: A poor surrogate for endogenous FMRP

Overexpressed autofluorescent-tagged versions of the Fragile mental retardation protein (FMRP) such as EGFP-FMRP have been used in protein-protein interaction studies and in studies of the composition, the formation and the localization of neuronal granules. However, the question of whether these molecules...

Author(s): Natalia Dolzhanskaya, Wen Xie, George Merz and Robert B. Denman

  • Article Number: 575130110880

January 2011

Virulence prediction model (virprob) using amino acid and dipeptide composition for human pathogens

Pathogenic bacteria that cause infectious disease are operated by various virulence mechanisms. Hence, it is important to develop a reliable system for predicting bacterial virulent proteins aiming at discovering novel drug/vaccine and for understanding virulence mechanisms in pathogens. On the basis of features like amino acid and dipeptide composition, it tried to identify the virulence potential in the given...

Author(s): S. B. Muley, V. Bastikar, S. Bothe, A. Meshram and N. Roy

  • Article Number: D6DA05E10890

July 2010

Possible role of 2, 2’- (Diazinodimethylidyne) di - (o-phenylene) dibenzoate, a novel hydrazine as an anti – HIV agent

A number of anti-HIV agents act by inhibiting specific steps in the lifecycle of HIV.  Inhibition of reverse-transcriptase (RT), a multi-functional enzyme is an important option. The interaction of 2, 2´-(Diazinodimethylidyne)-di-(o-phenylene)-dibenzoate (HZ) with HIV1-RT was studied to explore its possible use in anti-HIV therapy. This novel hydrazine derivative undergoes strong interaction with a number of...

Author(s): Rita Ghosh, Dipanjan Guha, Sudipta Bhowmik and Angshuman Bagchi

  • Article Number: 41932D740632

July 2010

Comparative study of inhibition of drug potencies of c-Abl human kinase inhibitors: A computational and molecular docking study

Structural studies suggest that the c-Abl protein kinase domain exists in two conformations; an active and an inactive form. There are many inhibitors which bind this tyrosine kinase in both forms. Many of these kinase inhibitors are in clinical trials too. The inhibition potency of these inhibitors is a common topic of discussion. In the present study we have taken a library of eight different inhibitors and docked...

Author(s): D. Kshatresh Dubey, K. Amit Chaubey, Azra Parveen and P. Rajendra Ojha

  • Article Number: 8C1BE0C40644

April 2010

BPES analyses of a new diffusion-advection equation for fluid flow in blood vessels under different bio-physico-geometrical conditions

In human physiological and pathological flow systems, it is not possible to rule out diffusion in all advective processes because perfusion goes hand in hand with diffusion processes. It is the perfusion throughout the capillary bed and then the diffusion of fluids throughout the tissue that is the subject of most magnetic resonance functional imaging procedures. It is observed from literature that basic theory of...

Author(s): M. Dada, O. B. Awojoyogbe, K. Boubaker and O. S. Ojambati

  • Article Number: 5D5103F10807

March 2010

Elements sequestered by arbuscular mycorrhizal spores in riverine soils: A preliminary assessment

The elemental composition of spore wall of arbuscular mycorrhizal fungi (AMF) was analyzed by energy-dispersive X-ray spectrometry (EDS) in a preliminary assessment. Measurements of AMF spores were made for riparian soils in both urban (rivers subjected to different human activities) and farm sites in southeastern Brazil. Spore populations belonging to four...

Author(s): M. C. Pagano, A. I. C. Persiano, M. N. Cabello and M. R. Scotti

  • Article Number: 402F89E10784

March 2010

Influence of gibberellic acid and arbuscular mycorrhizae inoculation on carbon metabolism, growth, and diterpene accumulation in Taxus wallichiana Zuccarini var. mairei

Changes in growth parameters and 14C02 and [U-14C]-sucrose incorporation into the primary metabolic pools and diterpene 10 DAB compound were investigated in leaves and stems of Taxus wallichiana Zucarnii treated with gibberellic acid (GA) and inoculated with arbuscular mycorrhizae (AM). Compared to the control, GA(1000 ppm) and AM (1 kg/ha each) with AM-GA combined treatments,  induced...

Author(s): A. Misra, N. K. Srivastava, A. K. Srivastava and S. K. Chattopadhyay

  • Article Number: D3301F210795

February 2010

Ultra-low doses of melafen affect the energy of mitochondria

Addition of the organophosphorous plant growth regulator - melafen to the mitochondria incubation medium resulted in modification of energy thereof. The modification was dose dependent one. The melafen concentrations 2 × 10-12, 2 ×10-18 and 2 × 10-21 М raised the maximum rates for oxidation of NAD+-dependent substrates, elevated the efficiency of oxidative...

Author(s): I. V. Zhigacheva, E. B. Burlakova, I. P. Generozova, A. G. Shugaev and S. G. Fattahov

  • Article Number: 4D568DE10753

February 2010

Determination of instantaneous arterial blood pressure from bio-impedance signal

The objective of this study was to determine the instantaneous arterial blood pressure by the peripheral bio-impedance. To achieve this goal, the equation of Ben Salah and Flaud, initially meant to determine the instantaneous aortic pressure according to the signal of thoracic bioimpedance, was successfully applied for the determination of the instantaneous arterial pressure according to the signal of peripheral...

Author(s): Sofiene Mansouri, Halima Mahjoubi and Ridha Ben Salah

  • Article Number: 6AB8EDC40590

December 2009

A thermodynamic investigation of bovine carbonic anhydrase II interaction with cobalt ion at 300 and 310K

A thermodynamic study on the interaction of bovine carbonic anhydrase II, CAII, with cobalt ions was studied by using isothermal titration calorimetry (ITC) at  27 and 37°C in tris buffer solution at pH = 7.5. The heats of Co2++CAII interaction are reported and analysed in terms of the new solvation theory. It was indicated that there are three identical and non-cooperative sites for Co2+. The binding of a...

Author(s): G. Rezaei Behbehani, A. Divsalar, A. A. Saboury and Z. Rezaei

  • Article Number: 81DB2C940683

December 2009

Chlorosoma: How can it contribute to photosynthesis of green bacteria?

Numerous kinetic data for excitation energy decay in bacteriochlorophylls as well as extremely short lifetimes of excitations in dominating chlorosoma pigment exclude the possibility of an efficient migration of this energy from chlorosoma to the main intra-membrane photosystem of some green bacteria. Author discusses the main purpose and molecular mechanism which account for this inconsistency in huge, 3D chlorosoma...

Author(s): A. Y. Borisov

  • Article Number: D6CE8A240691

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