Contribution of COVID-19 diagnosis in patients seen at the tuberculosis laboratory in the response to the COVID-19 pandemic in the Kayes Heath district
March 2024
The COVID-19 pandemic has posed challenges to tuberculosis (TB) services, leading to an increase in TB deaths for the first time in a decade. The aim of our study was to investigate the presence of SARS-CoV-2 in patients attending the Kayes Reference Health Center for tuberculosis diagnosis and anti-tuberculosis treatment monitoring. This was a cross-sectional and prospective study conducted at the Reference Health...
Resistance profile of isolated bacterial urinary tract infections in children in Abidjan from 2018 to 2020
February 2024
Urinary infection is a common pathology in children. It constitutes the second cause of pediatric consultation after respiratory infections. It is a public health problem, with 150 million cases of urinary tract infections recorded worldwide every year. The objective of this study was to update data on the prevalence of bacteria isolated from urinary infections in children and determine the evolution of sensitivity to...
Description of carbapenemase variants type OXA-181 and NDM-5 in clinical isolates of Enterobacteria in Senegal
January 2024
Antibiotic resistance is a global scourge. Carbapenems are one remedy for treating infections caused by extended-spectrum beta-lactamases (ESBL)-producing enterobacterales. Unfortunately, carbapenemase-producing enterobacterales (EPC) are emerging. In Senegal, the epidemiology of carbapenem resistance genes needs to be updated. From January 2019 to July 2022, 240 enterobacteria were selected from Fann Hospital (Dakar)...
Challenges and opportunities in advancing microbiology research in Africa: A review
October 2023
The need for efficient microbiology technologies in Africa has been a significant challenge to advancing microbiology research and study. This review examines the impact of this challenge and identifies the opportunities that exist to address them. These include the slow pace of research and development, the limited ability to identify and manage infectious diseases, and the challenge of monitoring and controlling food...
The prevalence of respiratory tract infections in the Ghaem Hospital of Mashhad
July 2023
Respiratory tract infection is a serious concern for public health worldwide, and imposes a lot of pressure on health facilities, specifically in developing countries along with economic restrictions. This study evaluated the prevalence of bacterial pathogens and their antibiotic sensitivity pattern among patients with respiratory tract infection in Ghaem Hospital, Mashhad, east of Iran during the 3 years period. All...
Prevalence and antimicrobial susceptibility of the borderline oxacillin-resistant Staphylococcus aureus (BORSA) strains in Bamako, Mali
March 2023
The borderline oxacillin-resistant Staphylococcus aureus (BORSA) strains have a low-level resistance to penicillinase-resistant penicillins. The aim of this study was to study the prevalence and the antimicrobial susceptibility of BORSA strains in Bamako. A retrospective study was conducted at the University Teaching Hospital of the Point G in Bamako. The S. aureus strains were isolated on Columbia agar supplemented...
Characterizing sweet potato RDR, AGO and DCL genes and potential involvement in defense against virus infections in sweet potato
March 2023
Characterizing anti-virus genes in sweetpotato is a vital step in mitigating yield loss due to virus infections. This work lays an insight into the structure and expression of key anti-virus genes. Related plant-based anti-virus genes were used as reference to mine key sweetpotato genes from various databases. BLASTN and BLASTP for transcripts was done for evaluation of phylogenetic relationship. Eight genes were...
Multidrug resistant bacteria isolated from nosocomial infections at University Teaching Hospital of Point-G, Bamako, Mali
January 2023
An infection is said to be nosocomial or hospital if it is absent when the patient enters the hospital and it appears and develops at least 48 h late. The objective of this study was to determine the resistance phenotypes of bacteria isolated from nosocomial infections at the University Teaching Hospital of Point G. Urine, blood, pus, skin and bronchoalveolar fluid samples were taken in different units, and bacteria...
Emerging threats of antibiotic resistance in Salmonella typhi and Salmonella paratyphi A among enteric fever cases of Dhaka, Bangladesh
January 2022
Enteric fever is a severe public health threat because of the rising antibiotic resistance of Salmonella species in developing countries, especially in its endemic areas like Bangladesh. This retrospective study was aimed to assess the effectiveness of a range of 17 commonly used antimicrobials against Salmonella Typhi and Salmonella Paratyphi A isolated from 601 enteric fever cases in Dhaka, Bangladesh. Conventional...
Assessment of antimicrobial resistance in avian pathogenic Escherichia coli Strains isolated over four years in Tunisian poultry
January 2022
Avian pathogenic Escherichia coli strains (APEC) are responsible for major economic losses in poultry farms. The use of antibiotics has led to the emergence of resistant bacteria having direct impact on the food industry. In order to evaluate the resistance of 191 Tunisian APEC strains, we determined the antimicrobial resistance profile of these bacteria to 18 antibiotics by disk diffusion method. This study revealed...
High prevalence of multidrug resistant enterobacteriaceae isolated from wastewater and soil in Jos Metropolis, Plateau State, Nigeria
December 2021
The widespread emergence of antibiotic resistance, particularly multiple antibiotic resistance (MAR) among bacterial species has become one of the most serious challenges in environmental protection. Environmental bacteria are a reservoir of antibiotic resistance genes (ARGs) and a potential source of novel resistance genes in environmental organisms. In the current study, we investigated the high prevalence of...
Antimicrobial susceptibility of Pseudomonas aeruginosa strains in Bamako, Mali
September 2021
Pseudomonas aeruginosa is generally susceptible to antibiotics of the families of Beta-lactam, aminoglycosides and quinolones. The aim of this study was to evaluate the antimicrobial susceptibility of P. aeruginosa strains in Bamako, Mali. P. aeruginosa strains were isolated on Drigalski agar. Antimicrobial susceptibility testing was performed using the disc diffusion method on Mueller-Hinton agar. Among 317 non...
Bacteriological load analysis of Moringa oleifera Lam. leaves consumed in Guinea Savannah vegetation zones of Nigeria
January 2021
The aim of this study was to evaluate the bacteriological load in Moringa oleifera Lam. leaves consumed in Guinea Savannah vegetation zones of Nigeria, via: Abuja (Gwagwalada market), Southern Guinea Savannah; Katsina (Daura market), Northern Guinea Savannah and Sokoto (Central market), Sudan Guinea Savannah. Three fresh and dried M. oleifera Lam. leafy samples each of 50 g were randomly collected per market location...
Prevalence of Escherichia coli O157:H7 in some animal products sold within Sokoto Metropolis, Nigeria
January 2021
Escherichia coli O157:H7 is among the most pathogenic of all known foodborne pathogens. It causes severe diarrhoea with apparently low infective dose (< 10 cells). This study aimed to determine the prevalence of E. coli O157:H7 in foods of animal sources sold in Sokoto Metropolis, Sokoto, Nigeria. A total of 175 samples were collected from different locations within Sokoto metropolis. Culture and biochemical...
Multidrug-resistant tuberculosis in HIV-negative patients in Lagos, Nigeria
November 2020
Multidrug-resistant tuberculosis (MDR-TB) is a big challenge to the tuberculosis control programmes worldwide. The aim of this work is to determine the rate of MDR-TB in HIV-negative patients with signs and symptoms of pulmonary tuberculosis in Lagos State. 891 adult HIV-negative patients enrolled for the study and tested for acid-fast bacilli by culture; resistance to first-line anti-tuberculosis drugs was performed...
Next generation sequencing platforms for potato virus hunting, surveillance and discovery
June 2020
Potato (Solanum tuberosum L.) is a key alternative to maize crop in Kenya. However, pests and diseases affect the yields. Information on Irish potato virology is continually patchy. Viral disease dynamics require constant updating to track new and novel agents. Efforts to mitigate viruses and crop breeding for tolerance can be determined this way. In Kenya, key potato viruses include: Potato Leaf Roll Virus (PLRV),...
Genetic diversity and resistance profile of mycobacterial strains isolated in Senegal
December 2019
Tuberculosis remains a major public health problem in Senegal. The aim of this study was to determine the resistance and molecular profiles of mycobacterial strains isolated over a four-year period in Senegal. Mycobacterial isolates worked on were obtained at the National Reference Laboratory of the National Antituberculosis Program (NAP) in Senegal. These strains were cultured on Lowenstein-Jensen (LJ) medium or on LJ...
Occurrence of biofilm producing bacteria in packaged water sold in Lagos, Nigeria
April 2019
Bacterial biofilms have been linked with protracted and recurring human infections, and considering that biofilms shed microorganisms into their environment it becomes pertinent to examine their role in waterborne diseases through consumption of treated packaged water. To evaluate the consortia of bacteria in packaged water for the ability to form biofilms. Using membrane filtration technique polythene sachet and pet...
Multidrug resistant Escherichia coli isolated from asymptomatic school going children in Kibera slum, Kenya
October 2018
Pathogenic Escherichia coli are of different types, currently grouped into six groups depending on the virulence gene(s) they possess. This study isolated pathogenic E. coli from 580 stool samples obtained in the month of August, 2016. The samples were obtained from asymptomatic school going children in one of the biggest urban slums in Kenya. Ten primary schools were randomly sampled and 40 to 80 stool samples...
Detection of Mycobacterium bovis in whey, by multiplex polymerase chain reaction (PCR) and bacteriological culture
October 2018
The objective of this study was to detect the presence of Mycobacterium bovis (M. bovis) in whey. A total of 233 cow milk samples were analyzed together with 26 tank milk samples that came from dairy herds of several states of the Mexican Republic (Querétaro, San Luis Potosí, Guanajuato, Hidalgo, Coahuila). DNA was obtained from whey and used for polymerase chain reaction-multiplex (PCR-M). Tuberculosis...
Antibiotic resistance genes in diarrheagenic Escherichia coli (DEC) isolated from livestock organic wastes in Ouagadougou, Burkina Faso
September 2018
Diarrheagenic Escherichia coli (DEC) are often disseminated through the fecal matter of livestock and waste products including slurry and manure. The study aimed to characterize archived DEC recovered from cattle fecal matter, manure and slurry for quinolone resistance and extended spectrum beta-lactamases (ESBLs) with focus on trends in antimicrobial susceptibility patterns. The susceptibility of the bacteria was...
In vitro assessment of Leuconostoc mesenteroides zinc nanoparticles against Salmonella serovars recovered from broilers chickens
September 2018
Salmonella serovars are responsible for a variety of acute and chronic diseases in poultry. Leuconostoc mesenteroides probiotic can exert antimicrobial activity by producing diverse fermentative metabolites with bactericidal or bacteriostatic activities such as lactic and acetic acids, fatty acids, hydrogen peroxide or diacetyl and antimicrobial proteins such as bacteriocins and peptidoglycan hydrolase enzymes. This...
Antibacterial and antioxidant activities of crude aerial part extracts of Satureja Punctata (Benth) Briq
September 2018
This study evaluates the antimicrobial and antioxidant activities of the crude extract of an aerial part of Satureja punctata (Benth.) Briq. The dried aerial part of S. punctata (Benth.) Briq was extracted separately using aqueous, ethanol and n-hexane. The antibacterial activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa was assessed using disc diffusion method, and the minimum...
Physicochemical analysis and microbial quality of cow butter obtained from Menz district of Amhara region, Ethiopia
August 2018
Menz has long been known for its quality butter production but traditional milk products are generally reported to be of substandard quality. Therefore, this investigation was conducted to access physiochemical and microbial quality of butter from Menz district along the market value chain. The study was conducted by using laboratory analysis of physiochemical and microbial quality. The microbiological count data were...
Enteric bacterial communities associated with the Omubhira Stream in Kakamega County, Kenya
August 2018
The present study was undertaken to establish the distribution and diversity of enteric bacterial communities along the Omubhira stream and also determine if physico-chemical parameters influence their distribution in water in terms of total coliforms (TC) and Escherichia coli. Stratified random sampling was used and four strata with 15 selected sampling sites identified along the stream. Selection of the sampling sites...
Bacterial identification and drug susceptibility pattern of urinary tract infection in pregnant Women at Karamara Hospital Jigjiga, Eastern Ethiopia
July 2018
Urinary tract infections are the most common bacterial infections during pregnancy and if left untreated in pregnancy it may result in acute pyelonephritis, abortion, premature delivery, low birth baby and even still birth. Thus the aim of this study was to determine the bacterial profile and antibiotic susceptibility patterns of urinary tract infection in pregnant women at Karamara Hospital Jigjiga, Eastern Ethiopia. A...
An overview of epidemiology and etiology of bacteria associated with diabetic injuries and their dominant infection at Central Region, Riyadh, Saudi Arabia
July 2018
Diabetes mellitus (DM) is one of the most chronic and dangerous diseases worldwide and in the Saudi society in particular. Swabs (168) from DM injuries were collected from inpatient and outpatient departments. Analysis of variance revealed the high incidence of diabetic foot infections compared to other injuries by an average of 51 and 33, respectively. Adults’ category was significantly the highest age category...
Beijing and the T superfamily, the major families among multidrug-resistant (MDR) Mycobacterium tuberculosis isolates from Senegal
March 2018
To determine the molecular profile of the multidrug-resistant (MDR) strains of Mycobacterium tuberculosis isolated in Senegal. From 2011 to 2014, 43 MDR strains isolated at the National Reference Laboratory for Mycobacteria of the National Antituberculosis Program (NAP) were characterized by spoligotyping. Nine different spoligotypes were identified, three of which were unique, the other six forming clusters of two to...
Studies on antibacterial spectrum of extracts of stem bark of Azadirachta indica A. Juss on some clinical isolates associated with urinary tract infection
November 2017
This investigation was aimed at determining the antibacterial activity of extracts of stem bark of Azadirachta indica A. Juss on some clinical isolates. Results of preliminary analysis showed that the stem bark of A. indica possesses alkaloids, flavonoids, tannins, cardiac glycosides, phlobotannins and phenols. The ethanol extract produced higher antibacterial activity against all test microorganisms compare to the...
Antimicrobial activity of secondary metabolites obtained with different carbon sources at different stages of the Pseudomonas growth curve isolated from Fresnillo, Zacatecas, México mineral soils
August 2017
The obtention of secondary metabolites from bacterial filtrates has permitted the identification of new compounds with diverse biotechnological applications. These metabolites were generated under different conditions. Eighty-six strains of Pseudomonas were isolated from mineral soils of the central region of Zacatecas to determine if these generated secondary metabolites possess antimicrobial activity against...
An optimised in vitro protocol for mass production of Rhizophagus irregularis spores - for sustainable agriculture
July 2017
The Arbuscular Mycorrhizal Fungus (AMF) plays a symbiotic vital role in most of the plant species by maintaining health, nutrient availability, against water stress, heavy metal resistance, against dreadful diseases with higher yields. To reach the requirement of AMF, large scale production practices can be required to produce specific strain aseptic inoculum. Carrot roots were selected for mass production of...
Evaluating the antimicrobial potency of crude extracts of Psidium guajava bark, leaves of Vernonia amygdalina, Carica papaya and whole plant of Phyllanthus niruri against specific pathogenic bacteria
April 2017
The potency of hot water, cool water and ethanoilc extracts of bark and leaves of Psidium guajava, leaves of Vernonia amydalina, Carica papaya and whole plant of Phyllanthus niruri were assessed against isolates of Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans obtained from the Mampong Research Institute of Herbal medicine in the Eastern region of Ghana....
Evaluation of the antibacterial activities of leaf extracts of Achyranthus aspera
February 2017
The fresh leaves of Achyranthes aspera Linn. soaked with water is traditionally used to treat skin infection in Ethiopia. The leaves are also applied traditionally to stop bleeding during cutoff in local people of Ethiopia. The above activities of the plant might be associated with antimicrobial activities. The leaves collected from their wild habitat were air dried under shade and ground into fine powder. The leaf...
Antimicrobial resistance pattern of bacterial isolates from different clinical specimens in Southern Ethiopia: A three year retrospective study
January 2017
Emergence of antimicrobial resistance is a major public health problem worldwide, particularly in developing countries. The effectiveness of currently available antibiotics is decreasing as a result of increasing resistant strains among clinical isolates. The aim of this study was to determine the antimicrobial resistance pattern of bacterial isolate from different clinical specimens at Hawassa University Referral...
Screening of bacteria isolated from the environment for the capability to control mosquito larva
November 2016
Malaria fever is a major disease that has killed millions of people in tropical and subtropical regions of the world. Mosquitoes are vectors of this debilitating and fatal disease. Therefore, mosquito control becomes paramount using environmentally friendly biological agents instead of chemicals. Water samples obtained from the gutters and water logged areas and soil samples analyzed resulted in 18 different bacteria...
Antibiogram and the efficacy of leaf extract of Chromolena odorota (L.) R. M. King and H. Robinson on bacteria isolated from some swimming pools within Akure metropolis
November 2016
Microorganisms being cosmopolitan are widely distributed in nature and can as well be found in swimming pools. These microorganisms contaminate swimming pools and other recreational water through indiscriminate defecation, contamination from rodents and birds etc. making these sources of water threat to human life. Concurrently, the resistant to conventional antibiotics has also increased the danger on people that are...
Isolation and characterization of feather degrading bacteria from poultry waste
October 2016
This study was conducted to isolate, partially purify and characterize keratinolytic microbes and their enzymes for commercial exploitation. A feather degrading bacterial strain was isolated from soil where poultry feathers were dumped by the poultry farms as a waste product. The isolated bacteria grew well at 37°C and the pH of 7.5. This isolate showed maximum feather degrading activity at 37°C. The enzyme...
Phenotypic and genotypic analysis of vancomycin-resistant Enterococci strains isolated from different water sources
February 2016
The purpose of this study was to investigate the phenotype, genotype and relationships of vancomycin-resistant Enterococcib (VRE) strains isolated from Minjiang River of Fujian province, residence district pond, university fish farm, cesspool and sewer. PCR was used to confirm Enterococci and amplify antibiotic resistance genes. Relationships between different strains were determined by repetitive extragenic palindromic...
Morphometric and quantitative analysis of the intestine of Rattus rattus infected by Strongyloides spp.
January 2016
Strongyloidiasis is caused by Strogyloides spp., which occurs when the parasite reaches the intestines. It is estimated that more than 370 million people throughout the world are infected with this disease. The aim of this study was to analyze the morphometric alterations and variation in the count of goblet cells of the jejunum of sinantropic rodents infected by Strongyloides spp., and to compare with non-infected...
In vitro efficacy of certain botanicals and their effect on some biochemical properties of Pectobacterium carotovora susp. carotovora inoculated tomato (Lycopersicon esculentum L.)
December 2015
Tomato (Lycopersicon esculentum L.) is a member of family Solanaceae. It is herbaceous, annual to perennial, prostrate and sexually propagated plant with bisexual flower. Tomatoes are attacked by many kinds of pathogens such as fungi, insects, nematodes, bacteria, viruses and viroid. Among bacterial diseases, bacterial soft rot devastates this important crop causing a huge decrease in yield and a greater loss in produce...
Prevalence and characterization of NDM-1 and OXA-48 carbapenemase gene harboring Enterobacteriaceae in a tertiary care hospital, South India
October 2015
The occurrence of carbapenem resistance among Enterobacteriaceae has reached critical levels worldwide. The present study was carried out to screen for carbapenem resistance among Enterobacteriaceae isolates from blood, surgical wound and tracheal samples in different wards and intensive care units between the period of 2010 through 2012 in a teaching hospital attached to JIPMER, south India. A total number of 425...
Comparison between the Widal test and culturing technique in the diagnosis of enteric fever in Khartoum State, Sudan
June 2015
Salmonella infection is a common bacteria disease that causes enteric fever in humans. Presently, Widal test, stool, blood and urine cultures are the most diagnostic means of confirming Salmonella infection in humans since they are based on the isolation, identification and demonstration of the presence of antibodies in the serum. However, the stool and serum sample of an infected patient against the (O) and (H)...
Polymicrobial ventilator associated pneumonia and antibiotic susceptibility of bacterial isolates in a university hospital, Tabriz, Iran
June 2015
Ventilator-associated pneumonia (VAP) is a common infection, developed in intensive care units (ICU). Early diagnosis and appropriate selection of antimicrobial therapy is important to reduce the mortality rate of these patients. Bronchoalveolar lavage (BAL) fluid was obtained from 27 patients, who fulfilled the criteria for ventilator-associated pneumonia. Samples underwent cytological and bacteriological analysis....
Molecular and biochemical study of Anaplasma marginale in cattle in Wassit Province of Iraq
May 2015
This study was designed to investigate the prevalence of bovine anaplasmosis among cattle from various areas in Wassit governorate; the investigation was performed on 184 blood samples collected from suspected cattle suffering from fever (41°C), severe anemia, pale mucus membrane, progressive emaciation and drop in milk yield, including 85 male and 99 female cattle, aged from < 1 year to > 2 years, the samples...
Purification and characterization of tryptophan and indole-3-acetic acid produced by Serratia marcescens strain MCB associated with Oscheius sp. MCB (Nematoda: Rhabditidae) obtained from South African soil
May 2015
Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis are lethal to insect pests that attack plants. These EPNs are associated symbiotically with the two enterobacteria Xenorhabdus and Photorhabdus sp., respectively. The bacteria synthesize a range of insecticidal and antimicrobial metabolites which may be useful as agricultural pest control and medical disease control. Recently, EPNs...
Potential biodegradation of low density polyethylene (LDPE) by Acinetobacter baumannii
April 2015
Acinetobacter baumannii was isolated from municipal landfill area, Pallikaranai, Chennai, Tamilnadu. The degradation ability of the bacteria was determined by performing Fourier Transform Infrared Spectroscopy (FTIR). The by-products of polyethylene degradation were monitored by gas chromatography-mass spectrometer (GC-MS) analysis. The toxicity of degradation by-products of low density polyethylene (LDPE) was tested on...
Acinetobacter spp. in the patients and environment of University Hospital of Yopougon, Côte d’Ivoire, from 2007 to 2011
April 2015
The objective of this work was to study the epidemiology and antibiotic resistance of strains of Acinetobacter spp. in the University Hospital of Yopougon Abidjan. This work studied the Acinetobacter strains isolated from humans and environment of the hospital; they were preserved in the culture collection of the Laboratory of Bacteriology from January 2007 to December 2011. Isolation and identification were made by...
Effect of high temperature on viability of Lactobacillus casei and analysis of secreted and GroEL proteins profiles
April 2015
The bacterial heat shock response is characterized by the elevated expression of a number of chaperone complexes including the GroEL and the rate change of synthesis of certain proteins (total and secreted). In this work, after incubation at 45°C, total and secreted proteins profiles of stressed bacteria were found to be altered when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)....
Antibacterial activities of two medicinal herbs on Salmonella typhi isolates in Abakaliki, Ebonyi State, Nigeria: Improvement to herbal medicine
March 2015
The study on the antibacterial activities of some medicinal plants on Salmonella typhi isolates in Abakaliki Ebonyi State was conducted using agar diffusion method. The study shows that there is high level yield in the dried leaves than in the wet leaves of the experimental plants (W/D). The investigation reveals that phenol yield is 296% W and 67% D in bitter leaf while oil yield in Utazi is 1.900 W and 1.034 D; phenol...
Extended-spectrum-beta-lactamase producing uropathogenic Escherichia coli infection in Dhaka, Bangladesh
January 2015
Extended-spectrum-beta-lactamase (ESBL) producing Escherichia coli that cause urinary tract infection (UTI) is a burning issue. This study was carried out to detect extended spectrum beta lactamase producing E. coli isolated from patients presented with UTI. This cross sectional study was conducted in the Department of Microbiology at Dhaka Medical College, Dhaka from January to December 2005, a period of one year....
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