International Journal of
Plant Physiology and Biochemistry

  • Abbreviation: Int. J. Plant Physiol. Biochem.
  • Language: English
  • ISSN: 2141-2162
  • DOI: 10.5897/IJPPB
  • Start Year: 2009

IJPPB Articles

March 2018

Estimation of auxins and cytokinins requirements in sugarcane soma clones for effective in vitro regeneration procedure

Three sugarcane varieties were used in this experiment to optimize specific plant hormones. The present work was conducted in 2014 at Nuclear Institute of Agriculture (NIA), Tando jam. The experiment was designated with three sugarcane varieties (NIA-2012, NIA-105 and GULABI-95) obtained from Nuclear Institute of Agriculture (NIA) Tando jam. Regeneration of plantlets was compared under different concentration of auxins...

Author(s): Sardar Khatoon Solangi, Sadaf Tabasum Qureshi, Mukhtiar Khatoon Solangi, Nusrat Solangi, Altaf Hussain Solangi and Mehnaz Qamar  

February 2018

Allelopathic effects of alkaloid fraction of Crotalaria retusa Linn on growth and some biochemical parameters of bean seedlings (Phaseolus vulgaris

The study investigated the potentials of alkaloidal fraction of leaf and stem of Crotalaria retusa with a view to understanding its mechanism of allelopathy in bean (Phaseolus vulgaris) seedlings. The study involved collection of C. retusa (leaves and stems), identification, dried, pulverized and extracted with methanol to yield methanolic extract (MECR). Crude alkaloid fraction was prepared from MECR by a procedure...

Author(s): Ogunsusi M., Akinlalu A. O., Komolafe I. J. and Oyedapo O. O.

August 2017

Effect of jasmonic acid on some biochemical and physiological parameters in salt-stressed Brassica napus seedlings

The present study demonstrated the effect of jasmonic acid (JA) on pigments and vitamins involved in photosynthesis or other metabolic activities during salt stress in Brassica napus L. Exposure to different concentrations of NaCl decreased pigments such as total chlorophyll, β-carotene, and lycopene to significant low levels but interestingly salinity increased total protein of B. napus L. seedlings, which was...

Author(s): Harpreet Kaur, Geetika Sirhindi and Poonam Sharma

June 2017

Identification of Pisum sativum L. cytokinin and auxin metabolic and signaling genes, and an analysis of their role in symbiotic nodule development

The present work was designed to study the role of the phytohormones, cytokinin and auxin, in the symbiosis development between pea Pisum sativum L. and Rhizobium leguminosarum bv. viciae. To achieve this aim, the temporal expression patterns of the genes related to the cytokinin and auxin biosynthesis, perception, and response were studied. It was found that most of these genes showed specific transcriptional...

Author(s): Dolgikh, E. A., Shaposhnikov, A. I., Dolgikh, A. V., Gribchenko, E. S., Bodyagina, K. B., Yuzhikhin, O. S. and Tikhonovich, I. A.

May 2017

Breeding of parental and tolerant hybrids of Theobroma cacao L. to Phytophthora megakarya Bras. and Griff.

The development of resistant varieties can be done according to a genetic approach. Estimation of genetic parameters for the quantitative traits of cocoa genotypes (Theobroma cacao L.) was made from a 5 × 5 diallel mating design. The objective of this study was to identify tolerant genotypes to black pod disease (BPD) through genetic analyzes. The inoculation of the leaves of parental cocoa genotypes and their...

Author(s): Martine Louise Ondobo, Pierre Onomo Effa, Jude Ndjaga Manga, Jules Christian Kouam Djoko, and Pierre François Djocgoue,

April 2017

Biochemical and physiological constituents and their correlation in wheat (Triticum aestivum L.) genotypes under high temperature at different development stages

Wheat is the most popular and staple food for millions of people. It is severely affected by heat stress in many countries. Vegetative growth and reproductive phases in wheat differ in their sensitivity to temperature. Heat tolerant (GW-190) and Heat susceptible (J-2010-11) genotypes grown up to tillering and grain filling stages and Heat treatments (40°C and 45°C for 2h and 4h) were given using Heating House....

Author(s): H. R. Ramani, M. K. Mandavia, R. A. Dave, R. P. Bambharolia, H. Silungwe and N. H. Garaniya

August 2016

Influence of the position of flowers buds on the tree on somatic embryogenesis of cocoa (Theobroma cacao L.)

The recalcitrance of Theobroma cacao L. to somatic embryogenesis, due to non-adapted physiological and metabolical responses to environmental stress, limits its propagation. The present work aims to ameliorate somatic embryogenesis in T. cacao throughout a physiological approach. For this purpose, the influence of the position of flowers buds used as explants was evaluated. Flowers buds were collected from different...

Author(s): Rodrigue Pouengue Boutchouang, Olive Flore Zebaze Akitio, Audrey Germaine Ngouambe Tchouatcheu, and Nicolas Niemenak

July 2016

Evaluating postharvest characteristics of Apantu (Local False Horn) plantain for harvest indices determination

Plantain (Musa sp., genome AAB) is a major locally grown starchy staple food highly consumed by Ghanaians with a per capita consumption of 84.4 kg. However, the maturity index of the fruit has been dependent on various characteristics including angularity, fruit tip drying, etc. A physiological study was conducted on the fruit characteristics of Apantu at harvest to determine the appropriate maturity index to be used by...

Author(s): Dzomeku B. M., Sarkordie-Addo J., Darkey S. K. Bam R.K and Wuensche J

December 2015

Effect of production sites and seed age on seed quality parameters of malt barley (Hordium vulgare L.) varieties

Quality malt barley production, processing and marketing had paid a greater degree of attention among farmers, traders, malting and brewing factories in Ethiopia. there is no quantified research information available on effect of production sites, seed ageing and potential varietal differences in meet up basic seed and grain quality standards for malt. Hence, this study was designed to evaluate the effect of production...

Author(s): Tefera Regasa, Firew Mekbib and Firdissa Eticha

May 2015

Alteration in photosynthetic pigments, osmolytes and antioxidants in imparting copper stress tolerance by exogenous jasmonic acid treatment in Cajanus cajan

Jasmonic acid regulates various abiotic stress responses but its role in regulation of copper (Cu) homeostasis has been poorly studied. This present study was conducted to explore the potential of jasmonic acid (JA) to enhance copper (Cu) tolerance in Cajanus cajan seedlings in terms of photosynthetic pigments, osmolytes and antioxidants. Seeds treated with different concentration (0, 1 µM, 1 nM and 1 pM) of JA...

Author(s): Geetika Sirhindi, Poonam Sharma, Anil Singh, Harpreet Kaur and Mudaser Mir

April 2015

Effect of cassava brown streak disease (CBSD) on cassava (Manihot esculenta Crantz) root storage components, starch quantities and starch quality properties

Effect of cassava brown streak disease on cassava root storage components were studied on four Ugandan varieties with varying levels of tolerance. Significant differences (P<0.05) were observed with reductions of 30% in amylose content and 50% in amylopectin content of diseased compared to healthy plots. Average dry matter content of diseased plots was 25% higher as much as starch yield and starch content reduced by...

Author(s): Nuwamanya Ephraim, Baguma Yona, Atwijukire Evans, Acheng Sharon and Alicai Titus

April 2015

The physiological and chemical response of stone fruit rootstocks (Prunus L.) to sulphur application under two different soil textures

A pot experiment was conducted during 2014 seasons at the field of Seed and Plant Institute, Karaj, Iran, to study the effect of sulphur application (with and without thiobacillus) on the physiological and chemical response of stone fruit rootstocks (Prunus L.) including "Myrobalan","GF 677", "Penta" and peach seedling rootstock (native) grown on two selected calcareous and alkaline (with...

Author(s): M. Mirabdulbaghi

March 2015

Effect of different seed priming treatments and priming duration on biochemical parameters and agronomic characters of okra (Abelmoschus esculentus L.)

Maintaining optimum plant population is an important factor in maximizing crop production and productivity. Establishment of okra in the field could be improved by applying suitable priming treatment to seeds. The present investigation was undertaken to find out the effect of seed priming treatments and soaking durations on biochemical parameters and agronomic characters of okra seeds. Okra seeds primed with three...

Author(s): Hardeep Kaur, Neena Chawla and Mamta Pathak  

December 2014

Bio-physiological response of biofilter algal candidate Ulva sp. to different nitrogen forms and fluxes

The present study was carried out to study the long term effect of different nitrogen forms and concentrations (14.3, 28.6 and 57.1 µM N-NH4 and 214, 2360 and 3700 µM N-NO3) on the photosynthesis and relative growth rate (RGR) of Ulva sp. for five weeks. Nutrient-enriched seawater was supplied at an exchange rate of between 12.6 and 14.4 volumes per day. Relative growth rate was determined weekly....

Author(s): Yasser T. A. Moustafa, G. Bougaran, M. Callier and J. P. Blancheton,

July 2014

The determination of vitamin C, total phenol and antioxidant activity of some commonly cooking spices crops used in West Bengal

In the present study, vitamin C, total phenols and antioxidant activity of some common cooking spices crops used in West Bengal were analyzed. Ten (10) spices (onion, chilli, garlic, ginger, turmeric, mustard seed, cumin seed, clove, cardamom and cassia leaf) were selected in order to determine the concentration of ascorbic acid (AA), total phenol (TP) and antioxidant activity as DPPH radical scavenging activity...

Author(s): Manas Denre

June 2014

The physiological and behavioural responses of argan seedlings (Argania spinosa (L.) Skeels) to water stress in the semi-arid Western Algeria

This paper presents the behavior, morpho-physiological and biochemical responses of argan seedlings to three water regimes at 30, 60 and 100% of field capacity. Based on the results of the growth parameters, we use the best result obtained from height growth. The increase in the number of leaves and thorns is obtained in seedlings subjected to water stress levels of about 60 and 100% against the application of a severe...

Author(s): Benaouf Zohra, Miloudi Ali and Belkhodja Moulay

June 2014

Comparative changes in metabolism of Vigna radiata by foliar and root application of brassinolide at different concentrations

The present study shows the effect of the foliar and root application of brassinolide on growth and metabolism of Vigna radiata. For this purpose, three concentrations of brassinolide (1, 0.01 and 0.0001 ppm) were applied on the plant. Brassinolide (BL) solutions were applied as foliar and root treatments for 21 days after sowing (DAS). Samples were collected after two and four weeks of treatments. Content of total...

Author(s): Sana Riaz, Neelofer Hamid and Afshan Rahman

May 2014

Antioxidant defence mechanisms in chickpea (Cicer arietinum L.) under copper and arsenic toxicity

Chickpea (Cicer arietinum L.) is one of the oldest pulse crops cultivated from ancient time both in Asia and Europe. India is the largest producer of chickpea in the world, sharing 65 and 70% of production and total global area, respectively. The inhibition of plant growth and crop productivity by excess heavy metals is a global agricultural problem. Therefore, a pot experiment was conducted at wire house of the...

Author(s): Shashi Kumar, Surender Kumar, Pravin Prakash and Mahendra Singh

April 2014

The effects of zinc (Zn) and C14-indoleacetic acid (IAA) on leaf senescence ın Helıanthus annuus L.

Sequential leaf senescence is defined as a kind of programmed death events which is an important process in growth of plant. This study aimed to explore the sequential leaf senescence rate due to indoleacetic acid and lack of zinc (-Zn). Therefore, the effect of zinc and indole-3-acetic acid on senescence which occurs in Helianthus annuus (sunflower) cotyledons was analyzed. It was found that in cotyledons of seedlings...

Author(s): Serap Sağlam-Çağ and Yener Okatan

April 2014

Interactive effects of virus and Rhizobium inocula on nodulation, growth and yield of cowpea

The effect of separately inoculating cowpea cultivars, ‘Ife brown” (IF) and “Owode” (OW) with Cowpea aphid-borne mosaic virus (CABMV), Cowpea yellow mosaic virus (CYMV) and Rhizobium IRc 284(RH-284) on nodulation was investigated. Also, the effect of inoculating the cowpea cultivars with RH-284 on the severity of infection caused by the viruses was studied. The interactive effects of inoculating...

Author(s): L. B. Taiwo, M. A. Taiwo, S. A. Shoyinka, S. E. Jegede, J. A. Okogun, O. S. Oyatokun and G. G. Adebayo

February 2014

Changes in antioxidants and pungency in response to foliar applications of Mn and Fe in onion

The aim of the present work was to study the changes in antioxidants and pungency in response to foliar applications of Mn [manganese: Mn1(0%); Mn2(0.5%); Mn3(1.0%)] and Fe [iron: Fe1(0%); Fe2(0.5%); Fe3(1.0%)] in onion genotype Sukhsagar (Allium cepa L.); a field experiment in factorial randomized block design with three replications was conducted in research farm of Bidhan Chandra Krishi Viswavidyalaya, Kalyani,...

Author(s): Manas Denre, A. Bhattacharya, S. Pal, A. Chakravarty, A. Chattopadhyay and D. Mazumdar

January 2014

Responses of grapevine rootstocks to drought stress

Drought response of rootstocks is important for overall viticulture as a result of increase in water scarcity due to climate change and irrigation limitations in winegrowing region. Grapevines are generally well-adapted to arid and semi-arid climates, and they appear to primarily rely on drought avoidance mechanisms in water stress situations. In terms of the response of the grapevine to drought conditions, rootstock...

Author(s): Dargie Tsegay, Dor Amsalem, Manuel Almeida and Molly Crandles

January 2014

Growth and photosynthesis response of the green alga, Picochlorum oklahomensis to iron limitation and salinity stress

The extreme environment of the Great Salt Plains (GSP) results in low algal biomass, such that natural selection is likely driven by survival of multiple abiotic stresses rather than rapid growth and biotic interactions. The objective of this study was to determine the effect of combined salinity stress and iron limitation on growth and photosynthesis in the green alga, Picochlorum oklahomensis isolated from the GSP...

Author(s): J. Nana Annan

November 2013

Crassulacean acid metabolism (CAM) in leaves of a common weed Commelina nudiflora L.

Survey of weed flora of Baramati region has revealed that Commelina nudiflora L. is a dominant weed among common weeds in croplands and fallow lands. In order to ascertain the survival potential of C. nudiflora some photosynthetic parameters along with water relations of this plant were investigated. Baramati region is a drought prone hot semi-arid area in Pune, Maharashtra. C. nudiflora,...

Author(s): R. B. Deshmukh and C. V. Murumkar

November 2013

Chemical analysis of leaves of Abrus precatorius

Leaves of Abrus precatorius are sweet and traditionally used to treat cough, malaria, snake bites and boils. This study evaluates the proximate and mineral composition of A. precatorius, and establishes the best solvent for the extraction of the sweet component of the leaves, by performing organoleptic test on the extract of different solvents, under different temperature conditions. The proximate...

Author(s): Paul E. D., Sangodare R. S. A., Uroko R. I., Agbaji A.S. and Dakare M. A.  

October 2013

Role of gibberellic acid (GA3) in improving salt stress tolerance of two wheat cultivars

Several environmental factors adversely affect plant growth and development and final yield performance of a crop. Drought, salinity, nutrient imbalances and extremes of temperature are among the major environmental constraints to crop productivity worldwide. Gibberellic acid (GA3)treatment has alleviated the drastic effect of salinity in growth parameters (leaf area, dry weight of grains and photosynthetic...

Author(s): Shaddad M. A. K., Abd El- Samad H. M. and Mostafa D.

October 2013

The physiological response of wheat plants to exogenous application of gibberellic acid (GA3) or indole-3-acetic acid (IAA) with endogenous ethylene under salt stress conditions

Wheat (Triticum vulgaris) plants were grown without NaCl and under salinization levels of NaCl. Salinity decreased the fresh, dry matter, water content, length and leaf area. Phytohormonal treatments with gibberellic acid (GA3) or indole-3-acetic acid (IAA) caused a marked increase in these parameters; GA3 was more effective than IAA. This activation was concomitant with the increase of osmotically active solutes,...

Author(s): Hamdia M. Abd El-Samad

September 2013

Effects of 28-homobrassinolide on growth, photosynthesis and essential oil content of Satureja khuzestanica

Brassinosteroids are a group of plant hormones that have significant biological effects on plant growth and development. The effects of 28-homobrassinolide (HBR) on growth, photosynthesis, chlorophyll content, carbohydrate fractions and essential oil content of savory herbs (Satureja khuzestanica Jamzad) were investigated. Foliar application of 28-homobrassinolide at 10-10, 10-8and 10-6 M...

Author(s): Mahdi Eskandari and Ali Eskandari

September 2013

The potential of Moringa oleifera extract as a biostimulant in enhancing the growth, biochemical and hormonal contents in rocket (Eruca vesicaria subsp. sativa) plants

Moringa oleifera is a highly valued plant, distributed in many countries of the tropics and subtropics. It has an impressive range of medicinal uses and high nutritional value. Accordingly, rocket (Eruca vesicaria subsp. sativa) plants were foliar sprayed with the aqueous extracts of leaves and twigs of M. oleifera at rates of 1, 2 and 3%. Among these concentrations, fertilization of rocket plants with 2%...

Author(s): Mona M. Abdalla

February 2013

Vegetation types and rangeland species nutritional values and forage quality indicators at various phonological stages

Information on different rangeland plants’ nutritive values at various growth stages is important in rangelands management. This information helps rangeland managers to choose proper grazing times to achieve higher animal performance without detrimental effects on the rangeland vegetations. Effects of various plant parts’ growth stages and vegetation types on reserve carbohydrates and forage quality...

Author(s): Reza Dehghani Bidgoli and Abulfazl Ranjbarfordoei

February 2013

Changes in some biochemical characteristics in response to foliar applications of chelator and micronutrients in green pungent pepper

In order to study the changes in some biochemical characteristics in response to foliar applicationsof chelator [humic acid: HA1 (0%) and HA2 (0.05%)] and micronutrients [manganese: Mn1 (0%); Mn2 (0.05%) and molybdenum: Mo1 (0%); Mo2 (0.01%)] in green pungent pepper cv. Bullet (Capsicum annum L.), a pot experiment in factorial randomized block design with three replications was...

Author(s): Manas Denre, P. K. Bandopadhyay, A. Chakravarty, S. Pal and A. Bhattacharya

January 2013

Cyanamide in plant metabolism

The calcium (Ca)-cyanamide is a nitrogen fertilizer with herbicide and fungicide side effects. Hydrogen cyanamide is absorbed as a molecule by roots and shoots of plants. It will be decomposed rapidly by the enzyme cyanamide hydratase to urea followed by urease to ammonium. From cyanamide decomposition mainly glutamate and alanine are built and the basic amino acids such as arginine, lysine and histidine are increased....

Author(s): A. Amberger

January 2013

Total lipid content in macrophytes of Wular lake, A Ramsar site in Kashmir Himalaya

The purpose of this study was to evaluate total lipid content in 15 fresh water macrophytes belonging to four ecological groups and examining the possible variations between the studied periods. The samples were obtained from nine sites throughout the Wular lake, during March to December 2011, spelling four seasons with determinations of lipid content of the aquatic macrophytes. No gross differences in the  lipid...

Author(s): Naseer Ahmad Dar, Aadil Hamid, Ashok K. Pandit, B. A. Ganai, Sami Ullah Bhat, and Arshid Hussain

October 2012

Effects of nitrogen and irrigation interval on broomrape (Orbanche aegyptiaca) damage reduction in host plant (Cucumis sativa L.)

In order to study the effects of nitrogen and irrigation interval on broomrape (Orbanche aegyptiaca) damage reduction in cucumber (Cucumis sativa L.), an experiment with two factors, nitrogen (100, 200 and 300 Kg/ha) and irrigation interval (3, 6 and 9 day), in three replications was performed in the greenhouse of Northern Khorasan Agricultural and Natural Resource Research Center. The results obtained show that the...

Author(s): Mehrnoush Eskandari Torbaghan, Gholam Reza Raoufinezhad and Masoud Eskandari Torbaghan

October 2012

Effect of lead and nickel toxicity on chlorophyll and proline content of Urd (Vigna mungo L.) seedlings

The effect of Pb and Ni on chlorophyll a, b, carotenoids and proline content of Black gram (Vigna mungo L.) seedlings were evaluated under 10, 50 and 100 µM concentration. These concentrations significantly affected chlorophyll, carotenoid and proline content of Black gram as compared to control. Pb and Ni at 10 µM concentration resulted in less significant effect on chlorophyll, a, b and carotenoids....

Author(s): Gurpreet Singh, Rajneesh K. Agnihotri, Rajendra Sharma Reshma and Mushtaq Ahmad

October 2012

Induction of a C-repeat binding factor gene by calcium to resist low temperature stress in rice

Adaptation to stress is an important phenomenon in plants. C–repeat binding factor (CBF), an Apetela 2 domain transcriptional activator, helps the plant in combating cold stress. Here, we cloned and purified a CBF family gene (OsDREB1B) from Oryza sativa. This gene (OsDREB1B) is induced by calcium in absence of cold. The bioinformatics analysis reveals that the induction of OsDREB1B is probably mediated...

Author(s): Gopal Chowdhary and Chanakya Nath Kundu

April 2012

Influence of media and growth regulators on regeneration and morphological characteristics of strawberry cvs Kurdistan and Merck (Fragaria x ananassa Duch.)

To evaluate the best media and growth regulator combinations for the regeneration and morphological characteristics of strawberry, meristems of Kurdistan and Merck strawberry cultivars were cultured on Murashige and Skoog (1962),  Gamborg et al. (1968) and Nitsch and Nitsch (1969) media supplemented with various growth regulator combinations including B1: (BA 1.0 mg/l + IBA...

Author(s): Ali-Akbar Mozafari and Mohammad Gerdakaneh

April 2012

Phytotoxicity of Parthenium hysterophorus residues towards growth of three native plant species (Acacia catechu willd, Achyranthes aspera L. and Cassia tora L.) in Himachal Pradesh, India

Parthenium hysterophorus is a strong allelopathic plant which belongs to the family Asteraceae of flowering plants. It is a rapidly spreading invasive plant species in the state of Himachal Pradesh up to 2000 m altitude. The allelochemicals produced by P. hysterophorus showed powerful toxic effects on the growth of other plant species. These allelochemicals are either phytotoxic phenolics or...

Author(s): Kuldip S. Dogra and Sarvesh K. Sood

April 2012

Influence of the harvest age on fructan content and fructosyltransferase activity in Agave atrovirens karw pine

The effect of age on the composition and concentration of non-structural carbohydrate was determined in the “pine” or “head” of Agave atrovirens Karw. The influence of plant age on the 1-FFT enzymatic activity, the electrophoretic pattern of proteins and the non-structural carbohydrate yield were also determined to establish the optimum age for harvest. The carbohydrate composition of 6...

Author(s): González-Cruz Leopoldo, Jaramillo-Flores María Eugenia, Bernardino-Nicanor Aurea and Mora-Escobedo Rosalva

April 2012

Inter correlation between soil properties and growth of Azadirachta indica in various types of plantations of Jodhpur region (Rajasthan, India)

Physico-chemical properties do not vary rapidly for short distance in arid and semiarid regions in absence of plantation or some manual intervention. In case of barren land, it is difficult to enhance soil health and its productivity until and unless a large quantity of fertilizer and irrigation facilities are employed. Application of high dose of fertilisers and insecticides result in short-term increase in production,...

Author(s): Verma Puneet and Kishan Kumar V. S.

March 2012

Ecophysiological responses of Melaleuca species to dual stresses of water logging and salinity

The combined effects of salinity and water logging on growth and ecophysiological characteristics of three Melaleuca species were investigated in a glasshouse study. Salinity treatments were imposed from day 28 at 0.3, 0.8, 2 and 5 g NaCl kg-1 soil. Shoot Na+ concentration and Na+/K+ratio for M. thyiodes at all salt level and of M. nesophila at 5.0 g NaCl...

Author(s): Nurul Aini, Emmanuel Mapfumo, Zed Rengel and Caixian Tang

March 2012

Acidity of tank bromeliad water in a cloud forest, Cusuco National Park, Honduras

Phytotelmata are plant-held water bodies housing complex aquatic invertebrate communities drawing attention for their suitability as breeding sites for disease bearing mosquitoes, and the unique fauna occurring in these habitats. Despite the human and scientific relevance, relatively little information is available on the water characteristics in these habitats and these scarce references consist only of isolated point...

Author(s): Jocque M., and Kolby J. E.,

March 2012

Cassava cyanocarbohydrate metabolism and proposed prehistoric symbionts

Plant/animal symbiosis produces interacting plant food parts that bear selective pressure to produce specific behaviors that aid the plant’s reproduction and/or survival. The cassava plant is here proposed to have created such a transactional food part in the cyanogenic carbohydrate tubers that acts through a sirtuin-activating pathway. The effect of exclusive cassava cyanocarbohydrate metabolism at the cellular...

Author(s): Van K. Golay

March 2012

Protection against heat stress in wheat involves change in cell membrane stability, antioxidant enzymes, osmolyte, H2O2 and transcript of heat shock protein

Terminal heat stress causes an array of physiological, biochemical and morphological changes in plants, which affect plant growth and development. In present investigation, real time quantitative expression analysis of HSP90 gene in root showed a maximum of 2.5 fold increase in the transcript level during seed hardening stage. Similarly, in flag leaf 4.5, 4.3 and 6.5 fold increases in the transcript level were observed...

Author(s): Ranjeet R. Kumar, Suneha Goswami, Sushil K. Sharma, Khushboo Singh,Kritika A. Gadpayle, Narender Kumar, Gyanendra K. Rai, Manorama Singh and Raj D. Rai

March 2012

Comparison of essential oil composition in wild and cultivated populations of Thymus pubescens Boiss. & Kotschy ex Celak. from Iran

Thymus pubescens Boiss. and Kotschy ex Celak. (Lamiaceae) is a grassy and permanent herb which grows wild in some regions of Iran including West Azarbaijan province. Content, composition and antioxidant activity of the essential oils of both wild populations and cultivated ones were compared in this study. The aerial parts of T. pubescens at the beginning of the flowering stage were collected...

Author(s): M. Pirigharnaei, R. Heydari, S. Zare, J. Khara and R. Emam-Ali sabzi

February 2012

Efficacy of seawater salinity on osmotic adjustment and solutes allocation in wheat (Triticum aestivum) flag leaf during grain filling

Two wheat (Triticum aestivum L.) cultivars (salt sensitive cultivar, Gemmieza-9 and salt resistant cultivar, Sids-1) subjected to different seawater salinity (10 and 25%). Osmotic pressure (OP), osmotic adjustment (OA) and solutes accumulation (TSS, TSN, proline, organic acids, glycerol and inorganic ions (Na+, K+, Ca2+, Mg2+ and Cl-) were quantified in flag leaf during grain-filling ...

Author(s): Heshmat S. Aldesuquy, Zakaria A. Baka, Omar A. El-Shehaby and Hanan E. Ghanem

February 2012

Genotoxicity and molecular screening of proteins in root tip cells of Trigonella foenum- graecum (Fenugreek var- Azad) under cadmium stress condition

Plants are unique in their ability to serve as in situ monitors for environmental genotoxins.Trigonella is receiving global attention due to rare medicinal properties of significance. Cadmium is one of the most toxic environmental pollutant affecting absorption and transportation of essential elements, disturb the metabolism, growth and reproduction. Present experiment was designed to investigate the...

Author(s): Towseef Mohsin Bhat, Sana Choudhary, MYK Ansari, Alka, and Rumana Aslam

January 2012

Regulation and properties of glucose-6-phosphate dehydrogenase: A review

Glucose-6-phosphate dehydrogenase (G6PD) is the key enzyme of the pentose phosphate pathway that catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolactone in presence of NADP+. G6PD is an enzyme of vital importance because of its role in various haemolytic disorders and its potential as a regulator for various biosynthetic pathways. NADPH is the important product of the reaction and is used for the...

Author(s): Siddhartha Singh, Asha Anand and Pramod K. Srivastava

January 2012

Detrimental impact of triplet oxygen in biosphere

Oxygen is essential for the existence of all forms of life since it is an important constituent of almost all biologically important compounds including DNA and serves as blood purifier. Simultaneously oxygen also induces a number of degradation processes through the generation of reactive oxygen species like superoxide radical anion, singlet oxygen, hydroxyl radical, hydrogen peroxide etc. The reactive oxygen species...

Author(s): Sunakar Panda

January 2012

Comparative study of different protocols for isolation of RNA from polysaccharides and polyphenolics rich plant tissues

The isolation of high quality ribonucleic acid (RNA) from plant has been a difficult problem due to presence of high amount of polysaccharides, polyplenolics and nucleases that mediate RNA degradation. After comparative analysis of four popular protocols for RNA isolation, a modified method was developed which gave maximum yield with purity. Modified method is not only suitable for the isolation of intact RNA from...

Author(s): Subodh Gupta, A. K. Verma and Laxmi Chand

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