Variability assessment of seed traits in Jatropha curcas L. for improvement of oil yield
January 2014
Jatropha curcas has received considerable attention from researchers as a potential source of non-edible vegetable oil which is eminently suitable for production of liquid bio fuel, meeting international standards. For any tree improvement and breeding programme, study of variation among the populations is prerequisite as it helps in the detection of relative performance of various traits of economic value. 28 candidate...
Insilico docking studies and evaluation of volatile oil of Zingiber officinale Rosc. and Aripiprazole for antidepressant activity
December 2013
The objective of the study was to evaluate the volatile oil of Zingiber officinale Rosc. and Aripiprazole for antidepressant activity and to carry out the docking studies for their respective binding sites. Argus Lab 4.0.1 software (Mark A. Thompson, Planaria Software LLC, and Seattle, WA, USA) was used to perform the docking studies and the elevated plus maze, forced swim...
Application of multiple displacement amplification in detection of five events of genetically modified organisms
December 2013
In this study, multiple displacement amplification (MDA) was used for transgene detection, and proved to effectively improve the sensitivity of most fluorescence polymerase chain (PCR). Five events of genetically modified plants were chosen for detection in different plant species by MDA and real-time PCRs. Individual primer/probe systems based on each transgene target were used. The extracted DNA of each sample was...
Low genetic diversity of Hypophthalmus marginatus from the Tocantins River based on cytochrome b sequence data
November 2013
In this study, we used a portion of mitochondrial cytochrome b to evaluate the current genetic structure of the Hypophthalmus marginatus Valenciennes 1840, from the Tocantins River in the eastern Amazonia. Genetic diversity was measured in two downstream (Abaetetuba and Cametá), and two upstream (Tucuruí and Itupiranga) stocks under influence of the Tucuruí Hydroelectric Dam....
Multivariate analysis of some Ethiopian field pea (Pisum sativum L.) genotypes
November 2013
The information on the nature and degree of diversity in the genotypes is crucial for efficient utilization of existing genetic resources. Thirteen (13) field pea genotypes along with two standard and one local checks were evaluated in triplicate randomized complete block design for three consecutive years to estimate the genetic variability and identify superior genotypes that generate putative transgressive...
Role of apoptosis-related factors in follicular atresia
October 2013
Follicular atresia is the term used for the fate of follicles which undergo degenerative changes before rupturing during ovulation. Recent studies suggest that granulosa cell apoptosis play a major role in follicular atresia. The factors which lead the cell to apoptosis and which protect the cell death, still remain complicated and more studies are needed to elucidate the whole process. Here in this review, we aimed to...
Effects of organic insecticides, Kingbo and Azdar 10 EC, on mitotic chromosomes in root tip cells of Allium cepa
October 2013
In this study, two organic insecticides (Kingbo and Azdar 10EC) extracted from plants and used as agricultural pesticides were investigated for cytotoxic and genotoxic effects using root tips of Allium cepa assay. Three different concentrations (0.625, 1.62 mL.L-1 and 2.5 mL.L-1) were used for different periods of time (8, 16 and 24 h). A single treatment of the effects of the two organic...
Inheritance of primary yield component traits of common beans (Phaseolus Vulgaris L.): Number of seeds per pod and 1000 seed weight in an 8 × 8 diallel cross population
September 2013
Thirty six (36) genotypes (eight parents and 28 F1 diallel crosses) were grown in randomized complete block design with two replicates during 2006 at Mandura, North western Ethiopia. The experiment was executed to study the inheritance of two primary yield component traits: number of seeds per pod and 1000 seed weight. Statistical significant difference was observed between genotypes, parents and crosses for...
Molecular characterization of cotton using simple sequence repeat (SSR) markers and application of genetic analysis
September 2013
Cotton is grown worldwide for the production of fiber and an important oil seed crop. Genetic diversity and correlation between varieties are of great importance for cotton breeding. To find out polymorphism and genome changeability, DNA markers are most comprehensively used. The current work was conducted to study the genetic relatedness among 20 cotton genotypes using simple sequence repeats markers using...
Two genotypes of Xanthomonas oryzae pv. oryzae virulence identified in West Africa
August 2013
Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo), is a very destructive rice disease worldwide. The aim of the present study was to examine if the Xoo virulence pathotypes obtained using phenotypic pathotyping could be confirmed using molecular approach. After screening of 60 Operon primers with genomic DNA of two Xoo isolates (virulent pathotype, Vr and mildly virulent pathotype, MVr), 12...
The genetic relatedness of drug resistant E.coli isolates of human and animal origin in Nigeria
August 2013
Molecular epidemiology of human and animal ecovariants of Escherichia coli from different regions of Nigeria were studied using their antibiotic susceptibility patterns, plasmid profile and pulsed-field gel electrophoresis (PFGE). E. coli was isolated using eosin methylene blue agar (EMB) and identified by conventional microbiological technique. The isolates were tested against 14 antibiotics using the disc diffusion...
Karyotype and meiosis analysis of four species of Cameroonian Pyrgomorphidae (Orthoptera)
April 2013
In this article, the karyotypic features (chromosome number, morphology, size and length and length of X chromosome), and meiosis in Atractomorpha lata, Dictyophorus griseus,Taphronota thaelephora and Zonocerus variegatus (Orthoptera: Pyrgomorphidae: Pyrgomorphinae) were analysed in order to determine similarities and differences amongst them. All four species were...
Interaction between angiotensin converting enzyme (ACE) insertion/deletion and aldosterone synthase (CYP11B2) -344C/T polymorphisms in relation to type 2 diabetes mellitus risk in Emiratis
April 2013
Polymorphism in Renin-Angiotensin-Aldosterone System (RAAS) genes have been studied extensively in various ethnic groups and largely with inconsistent findings on relationship with the risk of developing type 2 diabetes mellitus (T2DM). In this study, we investigated the association of Insertion/Deletion (I/D) polymorphism of angiotensin converting enzyme (ACE) and -344 C/T polymorphism of aldosterone...
Clinical features in a pediatric population due to chromosome deletions at a third level pediatric Mexican hospital in 19 years period of time: Five case reports
February 2013
Chromosome aberrations are considered as alterations in the chromosome number or structure. They are mainly due to gametogenesis inborn error (meiosis) or occur during the zygote first cellular divisions where DNA repair processes are deficient. Two Wolf Syndrome patients and two criduchat patients or patients with deletion of the short arm of chromosome 4 and 5, respectively, and deletion of chromosome...
Genetic variability, heritability and correlation in some faba bean genotypes (Vicia faba L.) grown in Northwestern Ethiopia
February 2013
A study was conducted at Dabat, Northwestern Ethiopia, during 2010 cropping season. Genotypic and phenotypic coefficient of variation, heritability and correlation coefficients were performed for yield and its contributing parameters in 10 faba bean genotypes. Analysis of variance for traits studied showed significant (P<0.01) differences among the genotypes. Phenotypic coefficient of variation values for...
Acute myeloid leukaemia associated to chromosome 6: Clinical and phenotypical implications in two pediatric patients with chromosome trisomy and translocation
November 2012
Chromosome aberrations are considered alterations in the chromosome number or structure. They are mainly considered due to gametogenesis inborn error or during the zygote first cellular divisions. Among 4617 chromosomal studies performed during 19 years (from 1992 to 2011), at Hospital Para El Niño Poblano in México, 34.6% (1596 patients) had chromosomal alterations. Among these study...
Evaluation of genetic heterogeneity in glutamate carboxypeptidase II (H475Y) and reduced folate carrier (SLC19A1) gene variants increased risk factor for the development of neural tube defects in eastern region of India
November 2012
In humans, neural tube failure to close during the 4th week of gestation leads to the development of severe congenital malformations of the central nervous system because of an error in maternal folate metabolism associated gene variants. The frequency of genotypic variants of GCP II (H475Y) and folate carrier RFCI (SLC19A1) gene polymorphism (80 G →A) were evaluated as potential candidate gene(s) and also assess...
Uraemic neuropathy: A review
December 2011
Uraemic neuropathy is an increasingly common condition, it is therefore important to understand its pathophysiology as well as its clinical manifestations. The objective of this study is to make a clear diagnostic approach and to provide treatment following the patient’s condition. Key words: Uraemia, dialysis, renal transplantation, kidney failure, chronic kidney disease (CKD)
Main chromosome aberrations among 4617 chromosomal studies at a third level pediatric Mexican hospital in 19 years period of time
December 2011
Mutations or chromosome aberrations are considered alterations in the chromosome number or structure. They are mainly considered due to gametogenesis inborn error (meiosis) or during the zygote first cellular divisions. All these alterations might be observed during metaphase from the cellular cycle, where DNA loses are seen (clastogenic processes) due to DNA repair processes deficiency o total absence, among...
The molecular aspects of oral mucocutaneous diseases: A review
November 2011
Most of the mucocutaneous diseases are confined to the stratified squamous epithelium and thus may involve skin, oral and other mucosae like the nasal, ocular, genital mucosa. Some patients present with oral lesions only whereas in others there may be involvement of skin and other mucous membranes. An understanding of the basic molecular aspects of these disorders is essential for proper diagnosis. Once a...
The Genetic variants of IL1RAPL2 gene associated with non-specific mental retardation in Chinese children
November 2011
The present study investigated the association between the genetic variants of IL1RAPL2 gene and Non-syndromic mental retardation (NSMR) in the children of QinBa region of China. Five common SNPs (rs5962434, rs5916817, rs3764765, and rs5962298 and rs9887672) of IL1RAPL2 were chosen and examined their individual genotype frequencies using the conventional polymerase chain reaction single strand conformation...
A questionnaire survey on infectious disease among hospital patients in Kushtia and Jhenaidah, Bangladesh
October 2011
A study on infectious disease is a qualitative study that used framework analysis to examine in-depth interviews among hospitalized patients. A pre-coded questionnaire was developed to obtain relevant information regarding socio-demographic status such as age, weight, family size, income per month, education, disease, health condition, dietary pattern, housing etc. The questionnaire was pre-tested before finalization....
IL 10 promoter polymorphism and gastric cancer risk in A+ve blood group patients in Kashmiri population
October 2011
Within the past few years, there has been increasing evidence that the genetic variation in the genes coding pro- and anti-inflammatory markers may play an important role in the pathogenesis of various human cancers, including gastric cancer. The aim of the study was to evaluate the association of Interleukin- 10 (IL-10)-1082 G/A and -592 C/A promoter polymorphism with gastric cancer in a North Indian...
Durable resistance in wheat
September 2011
Several rust resistance genes have been identified and used in breeding for resistance but new variants of the pathogen (referred to as races) overcome the resistance over a period of time. Most plant breeders and pathologists now advocate durable resistance to rusts based on multiple genes as best source of resistance as breeding for this type of resistance tends to produce long-lasting solutions. Wheat breeders...
TAP2 polymorphisms in Iranian patients with type I diabetes mellitus
September 2011
Type I diabetes (T1D) is an immune mediated disease characterized by immune destruction of insulin producing pancreatic beta cells. This disease is associated with the human major histocompatibility complex (HLA) region of the genome. The presentation of self peptides by HLA class I molecules is defective in individuals with this disease and both TAP1 and TAP2 are potential contributors to this defect. The...
Gas chromatography-mass spectrometry (GC-MS) analysis of petroleum ether extract (oil) and bio-assays of crude extract of Iris germanica
August 2011
Iris plants have immense medicinal importance and are therefore used in the treatment of cancer, inflammation, bacterial and viral infections (Nadkarni, 1976). Gas chromatography-mass spectrometry analysis of the petroleum ether extract (oil) of the plant have resulted in the identification of eleven compounds, 9-hexadecanoic acid methyl ester, 9-octadecenoic acid methyl ester, 8-octadecenoic acid methyl ester,...
Influence of risk factors on onset of hyperlipidemia in patients with cerebrovascular insult
August 2011
Evaluation of the association between ICAM-1 gene polymorphisms and sICAM-1 serum levels in multiple sclerosis (MS) patients in Southeast Iran
July 2011
Multiple sclerosis (MS) is an autoimmune nervous system disorder characterized by leukocytes recruitment into nervous system and demyelination. Intercellular adhesion molecule-1 (ICAM-1) mediates the extravasation of leukocytes and their accumulation in inflamed tissue. The aim of this study was to evaluate the probable association of ICAM-1 Exon 4 (G241R) and Exon 6 (E469K) gene polymorphisms with circulating levels of...
Five opitz G/B.B.B syndrome cases report with two chromosomal abnormalities; x chromosome duplication (47, XXY) and translocation 46XX t(3q;4q)
July 2011
Opitz G/BBB syndrome is a genetic condition that affects several structures along the midline of the body. The most common features of this condition are wide-spaced eyes (hypertelorism) with structural defects of the larynx, trachea, and/or esophagus causing breathing problems and difficulty swallowing (dysphagia). Some times in males, the urethra opening on the underside of the penis (hypospadias) is observed. Mild...
T-DNA direct repeat, vector backbone and gene trap counter selection by a new vector (pNU435) for high throughput functional genomics
June 2011
The nature of T-DNA/Ds insertion decides the utility of launch pad lines for iAc/Ds based insertional mutagenesis. Direct or inverted T-DNA/Ds repeats and insertions with vector backbone lead to poor recovery of flanking sequences; whereas T-DNA/Ds insertion leading to gene trap would limit the use of such launch pad lines. A new Ds tagging/trapping vector, pNU435 containing two copies of...
Platelet-derived growth factor (PDGF) signaling: Detailed mechanistic insights
June 2011
It is worth mentioning that large body of experimental evidence interconnects the deregulation of specific platelet-derived growth factor (PDGF) functions to cellular transformation, yet complete picture of determinants of molecular mechanisms through which PDGF contributes to tumorigenesis remain indefinable. In this review, we have summarized current comprehension concerning PDGF functions, and...
Genotype × environment interaction in NH47-4 variety of okra – Abelmoschus esculentus (Linn.) Moench
May 2011
Sixty lines of NH47-4 variety of okra (Abelmoschus esculentus), obtained from the National Institute of Horticultural Research and Training (NIHORT), Ibadan, Nigeria, were grown in two different locations (Akure and Ilara-Mokin, Ondo State, Nigeria) under rain-fed conditions using the Randomised Complete Block Design (RCBD) with four replicates. The plants’ genotypic coefficients of variation and correlations...
Morphological and molecular variation in Ethiopian lentil (Lens culinaris Medikus) varieties
May 2011
Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using nine agro-morphological characters and four ISSR primers. ANOVA of phenotypic data showed highly significant variation among the varieties for all traits. Number of pods and seeds, hundred seed weight, and number of secondary and primary branch showed high phenotypic coefficient of variation (PCV), while days to...
Effect of genotype, explant source and medium on in vitro regeneration of tomato
April 2011
For the present investigations, five cultivars including PKM-1, Moneymaker, Microtom, Micro-MsK and White Cherry of tomato (Lycopersicon esculentum Mill.) were selected. The explants cotyledon, hypocotyl and leaf of all the cultivars of tomato were cultured on MS medium fortified with different concentrations of Benzyl amino purine (BAP) (1.0 to 3.0 mg/L)and 0.1...
Plasmid-mediated transformation of Escherichia coli having a dependence on the heat-shock protein GroEL
April 2011
When the cells of Escherichia coli mutant in heat-shock regulator protein sigma-32 were transformed with plasmid pUC19 DNA, the transformation efficiency (TRE) of the mutant strain was about 10% of the wild type (wt) strain, indicating the role of heat-shock protein(s) in the transformation process. In order to search for the protein(s), when three different E. coli mutants in three important...
The differential expression of Rhopalosiphum padi resistance in sibling wheat-rye amphiploids
February 2011
Thirteen amphiploids were synthesized by chromosome doubling of an F1 plant of a cross between Triticum aestivum Mianyang11 × S. cereale Kustro, which shows resistance to Bird cherry-oat aphid (Rhopalosiphum padi). The amphiploids were confirmed by GISH analysis and tested for their reaction to R. padi. Eleven amphiploids exhibited resistance to R. padiand two of them exhibited...
Optical microscope gross characterization of rat neonatal testicular germline stem cell expansion in vitro
February 2011
Neonatal testicular germline stem cell suspension prepared from twenty 3-5 day old albino rats were incubated in normal saline or in NC-01in triplicate groups. A control group of saline alone (without cell suspension) was also prepared. The cell-containing groups and control were incubated for 21 days at room temperature, stained with 50% Mezo (organic) and visually studied daily with a view to histological...
Molecular docking study of tripeptides VPP and IPP inhibiting angiotensin I converting enzyme to alleviate high altitude pulmonary edema
January 2011
High altitude pulmonary edema (HAPE) remains the major cause of death related to high altitude exposure with a high mortality in absence of emergency treatment. Due to the deprival of oxygen in higher altitudes, the blood vessels constricts and squeezes blood in the vessels. This makes pressure to go up which in turn forces blood into air pockets in lungs that can kill people with HAPE. Angiotensin Converting Enzyme...
Application of subspecies-specific marker system identified from Oryza sativa to Oryza glaberrima accessions and Oryza sativa × Oryza glaberrima F1 interspecific progenies
January 2011
Interspecific hybrids (F1's) between Asian rice (Oryza sativa 2n=24 AA) and African rice (Oryza glaberrima 2n=24 AA) are almost completely sterile. This hybrid sterility barrier is mainly caused by an arrest of pollen development at the microspore stage. Intersubspecific F1 hybrid sterility is mainly caused by cryptic chromosomal aberrations and allelic interaction...
Chromosome complement and C-banding patterns in 6 species of grasshoppers
January 2011
Chromosomes with detailed karyotypic information (nature, number, size, relative length, length of X-chromosome, nature of X-chromosome) and C-banding patterns of six species of grasshoppers belonging to sub-families- Tryxalinae, Oedipodinae and Catantopinae are discussed. The karyotypes comprises of acrocentric chromosomes with complement number 2n=23 (male). Constitutive heterochromatin distribution was found at...
Genetic polymorphism BMP15 and GDF9 genes in Sangsari sheep of Iran
January 2011
Different mutations in the bone morphogenetic protein 15 (BMP15) and the Growth Differentiation Factor 9 (GDF9) genes cause increased ovulation rate and infertility in a dosage-sensitive manner in sheep. In this study, blood samples (140 ewes and 10 rams) were initially taken from 150 Sangsari sheep breed in Damghan animal breeding Centre using venojects treated with the anti-clot substance (EDTA) and subsequently...
Inheritance of ß-carotene in cassava (Manihot esculenta crantza)
December 2010
A study on the inheritance pattern of beta-carotene in cassava was carried out in the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria, using four parental lines of cassava, namely two yellow flesh and two white flesh colour genotypes. Crosses of yellow x white and white x yellow were made. The resulting progenies were screened and placed into distinct phenotypic classes and the data obtained were...
Rapid targeting and isolation of the β-like globin gene cluster fragment from AA, AS and SS genotypes using BamHI restriction enzyme
December 2010
The β-globin gene cluster contains the β-globin gene on which the substitution of the 17thnucleotide gives rise to sickle cell anemia and other β-globin gene variants with varying severity. Recent therapy measures focus on understanding the structure and mechanism of expression of the β-globin gene. In this study, we attempted to isolate a gene fragment containing the complete set of the...
Disorders of sexual development in genetic pediatrics: Three different ambiguous genitalia cases report from Hospital Para el Nino Poblano, Mexico
December 2010
Five pediatric patients with three different disorders of sexual development are reported in this study; the first three male patients (16 years, 4 years and 2 months old, respectively) were diagnosed as having diphallia. These 3 patients had real diphallia, well developed penises, urinarious meatus, and both testicles and one of the case, vessel duplication was reported by urology. All the patients have...
Tissue specificity in breast cancer: A mini review
November 2010
Cell proliferation by estrogen in the absence of BRCA1 protein may lead to a high mutation rate, thereby increasing the risk of acquiring cancer causing mutations (Scully and Livingston, 2000). The breast tissue is the target for estrogen and other hormones that are shown to endow antiapoptotic survival function upon cells sometimes in a non-autonomous manner. BRCA1 regulates DNA repair and apoptosis. In the...
Genotoxic effects of low 2.45 GHz microwave radiation exposures on Sprague Dawley rats
November 2010
This paper investigates the genotoxic effects of 2.45 GHz microwave (MW) radiation exposure at low specific absorption rates (SAR). 200 Sprague Dawley rats were exposed to SAR values between 0.48 and 4.30 W.kg-1 and the DNA of different tissues extracted, precipitated and quantified. Induced deoxyribonucleic acid (DNA) damages were assessed using the methods of DNA Direct Amplification of Length...
Four novel mutations detected in the exon 1 of MBL2 gene associated with rheumatic heart disease in South Indian patients
August 2010
The aim of this study was to determine the genetic variations associated with the mannose-binding lectin 2 (MBL2) gene in rheumatic heart disease (RHD) patients in the Vellore region, South India. This study included 50 patients with RHD and equal number of age and sex matched healthy controls. The genomic DNA was extracted from peripheral blood, to find out the genetic variations if any in MBL2 gene....
Healthy carrier parents in partial 7 and 9 chromosome trisomy in two pediatric patients: Report cases at the Hospital para el Niño Poblano, Mexico
August 2010
Two pediatric cases are reported in this study, a one year and five months old male patient with partial trisomy for the long arm of chromosome 9 due to chromosome segregation error in the father 46 XY, del (9q-)/46 XY dup (10q+), and a seven years old male patient with partial trisomy for the long arm of chromosome 7 due to chromosome segregation error in the mother 46 XX, ins (10; 7) (q21; q23q35) are...
Interleukin-1b gene polymorphism and its association with chronic periodontitis in South Indian population
August 2010
Interleukin-1 beta (IL-1b) is a potent inflammatory mediator and an important polymorphism in the locus +3954 (C/T) of the human IL1B gene has been shown to affect the levels of this cytokine. This functional polymorphism has been associated with the establishment of inflammatory diseases, including periodontal disease, in European, Asian and North American populations. The aim of this study was to...
Molecular phylogeny of Bulinus (Gastropoda: Planorbidae) reveals the presence of three species complexes in the Albertine Rift freshwater bodies
July 2010
In this study, partial mitochondrial DNA cytochrome oxidase subunit I (mtCOI) sequences (612 bp) of Bulinus snails sampled from 31 freshwater bodies in the Albertine Rift were analyzed to investigate the extent of genetic variation and phylogenetic relationships. Bayesian phylogenetic inferences clustered the samples into three species groups;Bulinus truncatus/ tropicus, Bulinus...
Page 2 of 3, showing 50 records out of 137 total, starting on record 51, ending on 100
Advertisement
Advertisement
