• 2017 May

• Genetic polymorphism of vesicular monoamine transporter 1 gene (SLC18A1) in Emirati population

Abstract

Vesicular monoamine transporters (VMATs) are an important target for biological research in neuropsychiatric disorders. Recent studies indicated that VMAT1 is expressed in the brain, thus making transporter plausible candidate genes for neuropsychiatric disorders. Furthermore, several recent genetic case-control studies have documented an association between common missense variations in the VMAT1 gene and... Read more.

• 2017 Apr

• Biodiversity of uncultured bacteria in hypersaline lakes, Siwa oasis, Egypt, as determined by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene phylotypes

Abstract

Bacterial populations within hypersaline lakes often exhibit uncultured unique species. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), followed by sequencing of 16S rRNA gene approach was applied in order to explore, for the first time, the bacterial communities within the hypersaline Lakes, Aghormy, Zeiton and Maraqi, Siwa Oasis, Egypt. The DGGE profile displayed 12 phylotypes of 16S rRNA... Read more.

• 2017 Mar

• The cichlid 16S gene as a phylogenetic marker: Limits of its resolution for analyzing global relationship

Abstract

The phylogenetic utility of the 16S gene in cichlids is assessed. Eighty-six (86) partial sequences belonging to 37 genera of cichlids from the Genbank was analyzed. The alignment had four hundred and sixty three (463) basepairs with 337 conserved sites and 126 variable sites. Base compositional bias is similar to that found in higher organism with Adenine having the highest average of 30.3%, followed by cytosine,... Read more.

• 2016 Aug

• Genetic diversity analysis in South African taro (Colocasia esculenta) accessions using molecular tools

Abstract

Taro [Colocasia esculenta (L) Schott] belongs to the family Araceae. It is an important staple food crop grown mainly by small scale farmers in many parts of the world. Taro is grown in South Africa from the coastal parts of the northern Eastern Cape to the KwaZulu-Natal north coast. Although it is an important staple in South Africa, very little information exists on the genetic diversity of the crop. Knowledge of the... Read more.

• 2016 Aug

• Comparison of Lagostomus maximus amelogenin gene (AMELX) with other mammals

Abstract

The aim of this study was to compare the Lagostomus maximus AMEL sequence to assess their similarity with the AMEL genes from other mammals. Previously, a sequence of the AMEL gene of L. maximus which corresponds to the intron 3 of the human sequence for AMELX (AMEL on the X chromosome) was reported. The comparisons made, by the use of the algorithm provided by the NCBI (Basic Local Alignment Search Tool: BLAST) allowed... Read more.

• 2016 Jul

• Recurrent mutation in the HMGCL gene in a family segregating HMG-CoA lyase deficiency

Abstract

The gene HMGCL encodes 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) lyase. Mutations in HMG-CoA lyase cause HMG-CoA lyase deficiency (HMGCLD), which is an autosomal recessive congenital disorder of metabolism. This study was designed to detect mutation in the 3-hydroxy-3-methylglutaryl-CoA lyase (HMGCL) gene in a Saudi family segregating HMG-CoA lyase deficiency (HMGCLD).Methods: Clinical and molecular genetic analysis of a... Read more.

• 2016 May

• Genotypic restriction patterns of finger millet (Eleusine coracana (l.) Gaertn) landraces in northern Nigeria

Abstract

The present study was conducted to characterize the genetic diversity of 10 germplasm accessions of finger millet (Eleusine coracana (L) Gaertn) collected from diverse locations spread across the geographical zone of Northern Nigeria.  The digest was carried out using the molecular marker Restriction Fragment Digest (RFD on agarose gel while EcoR1 and Hind III restriction enzymes were used to cut the genomic DNA at... Read more.

• 2016 Jan

• Association of the extracellular superoxide dismutase Ala40Thr polymorphism with type 2 diabetes mellitus and its complications in Lebanese population

Abstract

Free radical-induced damage plays a significant role in the development of type 2 diabetes and its complications. Extracellular superoxide dismutase (EC-SOD) or SOD3 is one of the important scavenger enzymes that protects against oxidative damage and the formation of free radicals. EC-SOD is a copper- and zinc-containing secretory enzyme located in tissues and primarily in the extracellular matrix. EC-SOD has been... Read more.

• 2015 Aug

• DYS459, DYS391, DYS388 and DYS19 genetic loci have high allelic frequency in patients with prostate cancer

Abstract

Prostate cancer is a significant disease in men, and a large number of individuals would benefit if risk factors that increase the susceptibility to develop prostate cancer could be established, which could aid in the early detection of the disease which is crucial for successful treatment. The first objective of this study was detection of allele frequencies of 12 Y-chromosome short tandem repeat loci from Iraqian... Read more.

• 2015 Jul

• Bael, Aegle marmelos (L.) Correa, an Indian medicinal plant protects V79 cells against the genotoxic effect of doxorubicin

Abstract

Alleviation of doxorubicin (DOX)-induced cytotoxicity and genotoxicity by leaf extract of bael also known as Aegle marmelos (AME) was studied in cultured Chinese hamster V79 cells. The optimum protective dose of AME was determined by treating V79 cells with different concentrations of AME before exposure to 10 µg/ml DOX and then by evaluating the cell survival and micronuclei frequency in the cytokinesis blocked... Read more.

• 2015 Jun

• Estimation of genetic parameters for first-lactation test-day milk yield in Holstein Friesian cows fitting random regression models

Abstract

In this study, the effects of varying order of Legendre polynomials (LP) for permanent environmental (Pe) variance structure on the estimates of genetic parameters for first-lactation milk yield were evaluated fitting random regression (RR) test-day animal models. The total data set included 6850 test-day milk yield records from 800 first-lactation Holstein Friesian cows that calved between 1997 and 2013 and the... Read more.

• 2015 May

• Isolation and sequence analysis of a putative MerR-type-transcriptional regulator and a multidrug efflux protein of Bacillus circulans ATCC 21588: As potential targets of therapeutics

Abstract

Mercury-type transcriptional regulators (MerR-transcriptional regulator) and major facilitator superfamily (MFS) transporters usually form an important sensor-response transport system in many microorganisms. This system has been shown to be involved in the regulation and transport (efflux) of a wide and diverse array of secondary metabolites including antimicrobial agents, dyes, chemicals, metals and evem harmful... Read more.

• 2015 Apr

• Effects of cytokinin types and their concentration on in vitro shoot induction and multiplication of korarima

Abstract

Korarima (Aframomum corrorima (Braun) P.C.M. Jansen) is a herbaceous perennial plant that belongs to the family Zingiberaceae in which lack of a steady supply of quality planting material is one of the bottlenecks for the exploitation of the export potential. Thus, the use of micropropagation is suggested to alleviate these problems. In micropropagation, in vitro seed germination and multiplication of hypocotyls/shoot... Read more.

• 2015 Feb

• Evidence of over-dominance for sickle cell trait in a population sample from Buenaventura, Colombia

Abstract

Sickle cell disease is a hereditary disease caused by a hemoglobin variant, known as hemoglobin S. This hemoglobin variant has a high frequency in areas where malaria is prevalent, because heterozygous genotype has a protective action against malaria infection. We evaluated evidence of over-dominance or deviation from Hardy- Weinberg equilibrium for the HbS gene in Buenaventura, an afro-descendent population with actual... Read more.

• 2014 Nov

• Multivariate analysis of genetic divergence among Ethiopian mustard (Brassica Carinata A. Braun) landraces in Ethiopia

Abstract

Characterization of existing genetic variability is a prerequisite for further crop improvement activity. This study was designed to assess genetic variability among randomly selected Ethiopian mustard (Brassica Carinata A. Braun) genotypes from different agro ecology of Ethiopia. The present study was undertaken to determine nature of association of agronomic traits of 36 Ethiopian mustard (Brassica carinata) genotypes... Read more.

• 2014 Nov

• Genetic polymorphism of blood potassium in goat belonging to the different breeds in Mongolia

Abstract

In goats belonging to the different breeds and sub breed, the genetic polymorphism at the determinant locus of blood potassium was revealed by flame spectrophotometer method. The kalemic systems in those breeds were characterized by a polymorphism of middle level due to the existence of the two phenotypes and of three genotypes. The polymorphic character of this system is given by the distributional discontinuity of... Read more.

• 2014 Oct

• Use of simple sequence repeat (SSR) markers to establish genetic relationships among cassava cultivars released by different research groups in Ghanaian

Abstract

Cassava (Manihot esculenta) is an important staple crop widely cultivated in Ghana. The crop also has diverse industrial applications including starch, beer and alcohol productions. Knowledge about the state of the Ghanaian cassava genetic diversity and population structure is paramount in breeding programmes aimed at cultivar improvements or breeding of new cultivars for specific purposes. This study focused on the use... Read more.

• 2014 Mar

• Heliclobacter pylori associated chronic gastritis: Endoscopic and pathological findings, comparative study

Abstract

Helicobacter pylori have been established as a major etiologic factor in the pathogenesis of chronic gastritis, peptic ulcer disease and in the development of gastric adenocarcinoma and gastric mucosa-associated lymphoid tissue lymphoma. This study was conducted on 100 patients. All underwent upper endoscopy, and antral and corpus, and duodenal biopsies were taken. Findings of endoscopic gastritis were observed in 83... Read more.

• 2014 Mar

• Challenges in conserving and utilizing plant genetic resources (PGR)

Abstract

The problems of food and income security are of global significance and are further compounded by precedential increase in world population resulting in overexploitation of natural resources and by extension plant genetic diversity. Plant genetic resources (PGR) refer to the heritable materials contained within and among plant species of present and potential value. In the recent past, genetic diversity found in... Read more.

• 2014 Jan

• Partitioning and distribution of random amplified polymorphic DNA (RAPD) variation among eggplant Solanum L. in Southwest Nigeria

Abstract

Solanum L., the largest genus of the Solanaceae family, vary morphologically, is diverse in number and is ecogeographically distributed. In Nigeria, previous studies had focused mainly on chromosome morphology, genome description and medicinal values, which are insufficient for genetic affinities. This study used four highly polymorphic random amplified polymorphic DNA primers to describe both the genetic relatedness... Read more.