Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
Start Year: 2010
Page 1 of 5, showing 20 records out of 91 total, starting on record 1, ending on 20
DOI: 10.5897/IJBMBR2015.0241[Article Number: 926D78261087]
Fasciola spp. is responsible for huge economic losses and animal welfare issues within the agricultural sector worldwide. Protein profiling studies of the two economically important species of Fasciola, that is, Fasciola hepatica and Fasciola gigantica are important for identification of these species, their immunodiagnosis and also for vaccine designing. Protein profiling is expected to discover a number of target... Read more.
DOI: 10.5897/IJBMBR2012.018[Article Number: FFCF7AA60770]
Jatropha curcas L. is peculiar treasured tree species for its uses and considerable economic potential as a biofuel plant. Propagation using seeds is limited by low viability and germination hence unable to provide high quality planting material for sustainable use. To achieve mass production of J. curcas, regeneration from leaf discs and petioles using different treatments of plant growth regulators (PGRs) including 6-... Read more.
DOI: 10.5897/IJBMBR2015.0245[Article Number: 90909E059065]
Cassava (Manihotes culenta Crantz) is among major food and income security crops in sub-Saharan Africa. However, high seed dormancy and delayed germination limit seed propagation. Using traditional stem cutting causes loss of superior genotypes and decreases productivity as a result of low multiplication ratio (1:10) and viral and bacterial diseases.Thus, the aim of this study was to optimize and screen efficient in... Read more.
DOI: 10.5897/IJBMBR2015.0227[Article Number: B88C34F57886]
The aim of the research was to study the effect of adjusting the initial pH of shrimp shell (8.64 vs. 7.00) on the ability of the fungus Aspergillus niger to carry out deproteinization process of galactose supplemented shrimp shells and to investigate the performance characteristics of the deproteinization process. The results showed that the initial pH of the shells has a significant effect on the temperature, carbon... Read more.
DOI: 10.5897/IJBMBR2014.0207[Article Number: D23B6BA56446]
Legumes have been used as food by indigenous people of Africa yet their full potential as food sources has not been completely realized. With modern technologies such as marker-assisted selection and mutational breeding, there is scope for rapid improvement of germplasm in these underutilized species. One approach is to induce genetic mutations through irradiation of plant tissues with neutrons or gamma rays generating... Read more.
DOI: 10.5897/IJBMBR2014.0231[Article Number: A3E78B155990]
Explants of two elite cooking banana cultivars, FHIA17 and INJAGI were collected from healthy source of stock plants growing in the field. Sterilization was evaluated using different concentrations (20, 30, 40, 50 and 60%) of a commercial bleach (JIK) for 25 min. Effects of cytokinins benzyl amino purine (BAP), 2-isopentenyl adenine (2iP) and kinetin each evaluated at 5, 10, 20, 24 and 40 µM and thidiazuron (TDZ)... Read more.
DOI: 10.5897/IJBMBR2014.0213[Article Number: 13DDB2F53798]
The discovery of RNA interference by Fire and Mello has opened up a new arena for research in molecular biology. The interfering RNAs (iRNAs) are 21-23 nucleotide RNA duplexes, which have the ability to reduce or abolish gene activity, and this property is being used in reverse genetics. Dicer and RISC (RNA-induced silencing complex) play important role in mechanism of RNA interference. The specificity, efficiency and... Read more.
DOI: 10.5897/IJBMBR2014.0216[Article Number: 2DCEE8952892]
The present study was conducted with the aim of evaluating some of the factors that influence induction and regeneration of somatic embryos in Vitex doniana since there are no available reports on tissue culture of this tree species. Leaves from plants growing under temporary shed were cultured on Murashige and Skoog supplemented with silver nitrate and four amino acids (proline, tryptophan, lysine and leucine) at... Read more.
DOI: 10.5897/IJBMBR2014.0199[Article Number: 5C111FD51669]
Alkaline proteases are used in food industry, leather tanning and processing industry, preparation of pharmaceuticals and also in the fiber industry. An alkaline serine protease producing strain was isolated using soil sample from a natural hot water spring in Sri Lanka. It was identified based on morphological, biochemical and 16s rRNA identifications as Bacillus licheniformis NMS-1. The extacellular protease enzyme... Read more.
DOI: 10.5897/IJBMBR2014.0219[Article Number: 7DC1AB550698]
Genetic diversity assessment of genetic resources maintained at Gene-Banks has important implication for future improvement, conservation and collection activities. However, such information is not available for sesame collected by IBC, Ethiopia. Inter simple sequence repeat (ISSR) marker was used to assess the level of genetic diversity, genetic structure and genetic distance, and to indirectly estimate the level of... Read more.
DOI: 10.5897/IJBMBR2014.0218[Article Number: 927CE3249952]
Biodegradation is the chemical breakdown of materials by a physiological environment. The term is often used in relation to ecology, waste management and environmental remediation. A specific gene from bacteria is identified and sequenced which has the capacity of oil degradation without any obvious strain specific discrimination using a combination of PCR and hybridization. The parameters of biodegradation that is... Read more.
DOI: 10.5897/IJBMBR2014.0184[Article Number: A797DEA49386]
The identification of a microbial isolate to genus level only amounts to a partial characterization of the isolate, but this can tell us a lot about that organism. Knowing the species allows the laboratory access to the body of knowledge that exists on that species. Identification schemes using phenotypic characteristics such as colony and cell morphology, Gram reaction and other staining characteristics, nutritional... Read more.
DOI: 10.5897/IJBMBR2014.0204[Article Number: D0E922249388]
The Russian wheat aphid (RWA), Diuraphis noxia (Kurdjumov) causes extensive economic damage to wheat (Triticum aestivum L.) in most wheat growing regions of the world. Control of RWA using systemic insecticides is expensive and pollutes the environment therefore the most effective method of RWA control is the development of RWA resistant cultivars. This study was initiated to determine inheritance of RWA resistance in a... Read more.
DOI: 10.5897/IJBMBR2014.0192[Article Number: D43368046890]
Extrapulmonary tuberculosis is an important clinical problem particularly in developing countries. The aim of this study was to assess two different samples (blood and fluid) for the diagnosis of extra-pulmonary tuberculosis (abdominal tuberculosis and tuberculous lymphadenitis). The study subjects were recruited from WadMedani Teaching Hospital during 2009-2013. Seventy five ascetic fluid and blood samples were... Read more.
DOI: 10.5897/IJBMBR2014.0193[Article Number: FD8205B46891]
Studies on the effects of mutations within flavonoid pathway genes on the resultant flavonoid profiles in sorghum are important in the identification and characterisation of varieties with nutritionally superior flavonoid profiles. In this study, we aimed at determining the effect of mutations at one important flavonoid pathway locus, the anthocyanidin synthase (ANS) gene, on grain flavonoid profile in sorghum. Sequence... Read more.
DOI: 10.5897/IJBMBR2014.0191[Article Number: 16D975746312]
Some nutrients in livestock feed may not be fully digested by animals own digestive enzymes, and hence important nutrients are unavailable to the animal. Although, supplementation of enzymes to farm animals has shown to increase the digestibility of poorly digested diets to a much greater extent. By targeting specific anti-nutrients in certain feed ingredients, feed enzymes allow especially pigs and poultry to extract... Read more.
DOI: 10.5897/IJBMBR2014.0196[Article Number: 2B0F93546316]
Enzymes are substances produced by a living organism which acts as a catalyst to bring about a specific biochemical reaction. Amylases are a class of hydrolytic enzymes, widely spread in nature having varied application in different industrial processes and constitute a class of industrial enzymes. Fungal amylases have been widely used for the preparation of oriental foods. In spite of the wide distribution of amylases... Read more.
DOI: 10.5897/IJBMBR2014.0189[Article Number: C69E99A45501]
The inevitability of applications of antioxidants in protection of human and preservation of dormant living systems such as food, drugs, cosmetics and other allied chemicals against internal and external stresses have made antioxidants an area of active research world over. Study impact of antioxidant research has advanced from topic level to subject level as revealed by the volume of antioxidants research publications.... Read more.
DOI: 10.5897/IJBMBR2014.0186[Article Number: C73CF5E45008]
Humic acid promote the conservation of mineral nutritions and as well as stimulate the pungency usually being more prominent in the cloves of garlic bulb. The effect of humic acid application on accumulation of mineral nutritions and pungency in garlic (Allium sativum L.) cv. ‘Gangajali’ was studied. Here, we observed that the maximum concentration of Ca, Fe and S were shown when plants were treated with 400... Read more.
DOI: 10.5897/IJBMBR2013.0178[Article Number: 9C9B96443879]
The current study aimed at evaluating the effect of gibberellic acid (GA3) and sucrose on in vitro propagation of two elite sweet potato cultivars (Ukerewe and Gihingamukungu). Nodal explants from in vitro growing plantlets were harvested and cultured on Murashige and Skoog media supplemented with 2.5, 5, 10, 20 and 40 μM, GA3. In a separate experiment, sucrose was evaluated at 30, 60, 90, 120, 150, 180 and 210 mM.... Read more.
Page 1 of 5, showing 20 records out of 91 total, starting on record 1, ending on 20