International Journal of
Biotechnology and Molecular Biology Research

  • Abbreviation: Int. J. Biotechnol. Mol. Biol. Res.
  • Language: English
  • ISSN: 2141-2154
  • DOI: 10.5897/IJBMBR
  • Start Year: 2010

IJBMBR Articles

March 2018

Isolation, production and characterization of amylase enzyme using the isolate Aspergillus niger FAB-211

Amylase enzymes are industrially important enzymes used in food, sugar, textile, pharmaceutical, paper and detergent industries. The main objective of this study was to isolate, produce and optimize α-amylase enzyme using a fungal strain isolated from fruit peel soil wastes. Media optimization was done by one-factor at a time method. Average values of duplicate experiments were taken. Microsoft office Excel...

Author(s): Behailu Asrat and Abebe Girma

January 2018

Postharvest quality of tomato (Solanum lycopersicum) varieties grown under greenhouse and open field conditions

An experiment was carried out to evaluate performances of four tomato varieties (Bishola, Eshet, Marglobe and Moneymaker) harvested at ripe stage from greenhouse and open field condition at Jimma for post-harvest quality characters (total soluble solid, weight loss, titratable acidity, sugar-acid ratio, and pH). Bishola and Eshet had better chemical quality characteristics maintained throughout the end of the storage...

Author(s): Yebirzaf Yeshiwas and Kassaye Tolessa  

November 2017

Selection and optimization of lignocellulosic substrate for laccase production from Pleurotus species

The aim of the current study was to investigate the potential of Pleurotus species for laccase production on different lignocellulosic substrates and determine the optimal levels of physicochemical conditions required for the production. The study was conducted in National Agricultural Biotechnological Research Center, Holetta. The fruiting bodies of fungi were collected based on their morphology and inoculated on...

Author(s): Teshome Edae and Melaku Alemu

October 2017

Genotype by environment interactions and grain yield stability of released and advanced Desi type chickpea (Cicer arietinum L.) genotypes in western Ethiopia

Genotype by environment interaction (G×E) obstructs breeding by persuading variations in genotype performance in different environments and thereby complicating selection. The aim of the present study was to determine the stability and yield performance of desi type chickpea varieties and advanced lines at multiple growing environments of western Ethiopia, using genotype-by-environment interaction (GGE) biplot...

Author(s): Biru Alemu, Kassahun Tesfaye,, Teklehaimanot Haileselassie and Dagnachew Lule

September 2017

Evaluation of two Portuguese strains of Botryococcus braunii as biofuel feedstock

ACOI 58 and ACOI 1257, two different Portuguese strains of colonial green microalga Botryococcus bruanii Kutz obtained from Coimbra Collection of Algae (ACOI), were evaluated on their potential for biofuel production, which was assessed from batch culture in photobioreactors under indoors and outdoors conditions using pretreated waste water and semisynthetic culture media. In the experiments, the maximum specific growth...

Author(s): João Tavares, Tiago Pardal and Ricardo Melo,

June 2017

Callus growth and ion composition in response to long-term NaCl-induced stress in two sugarcane (Saccharum sp.) cultivars

In this work, the effect of different concentrations of NaCl on calli induced from two sugarcane cultivars NCo310 and CP59-73 was studied. Growth and ion concentrations (Na+, Cl-, K+ and Ca2+) of calli were determined after 1, 2 and 3 months of stress with the objective to understand the cellular mechanisms operating in salt stress tolerance and to determine the implication of inorganic fraction in salt tolerance in...

Author(s): Tomader Errabii, Christophe Bernard Gandonou, Samira Bouhdid, Jamal Abrini and Nadia Skali-Senhaji

October 2016

Studies on various types of antigenic proteins of Fasciola spp. as a basis for the immunodiagnosis and vaccine development: A review

Fasciola spp. is responsible for huge economic losses and animal welfare issues within the agricultural sector worldwide. Protein profiling studies of the two economically important species of Fasciola, that is, Fasciola hepatica and Fasciola gigantica are important for identification of these species, their immunodiagnosis and also for vaccine designing. Protein profiling is expected to discover a number of target...

Author(s): J. S. Dar, I. Tak, B. A. Ganai and R. A. Shahardar

September 2016

Efficient somatic embryogenesis of Jatropha curcas L. from petiole and leaf discs

Jatropha curcas L. is peculiar treasured tree species for its uses and considerable economic potential as a biofuel plant. Propagation using seeds is limited by low viability and germination hence unable to provide high quality planting material for sustainable use. To achieve mass production of J. curcas, regeneration from leaf discs and petioles using different treatments of plant growth regulators (PGRs) including 6-...

Author(s): Cecilia Mbithe Mweu, Aggrey Nyende and Justus Onguso

June 2016

Shoot nodal culture and virus indexing of selected local and improved genotypes of cassava (Manihot esculenta) from Sierra Leone

Cassava (Manihotes culenta Crantz) is among major food and income security crops in sub-Saharan Africa. However, high seed dormancy and delayed germination limit seed propagation. Using traditional stem cutting causes loss of superior genotypes and decreases productivity as a result of low multiplication ratio (1:10) and viral and bacterial diseases.Thus, the aim of this study was to optimize and screen efficient in...

Author(s): Janatu V. Sesay, Kwadwo O. Ayeh, Prince E. Norman and E. Acheampong

January 2016

Effect of the initial pH on the performance characteristics of the deproteinization process of galactose supplemented shrimp shells by Aspergillus niger in a solid state drum bioreactor

The aim of the research was to study the effect of adjusting the initial pH of shrimp shell (8.64 vs. 7.00) on the ability of the fungus Aspergillus niger to carry out deproteinization process of galactose supplemented shrimp shells and to investigate the performance characteristics of the deproteinization process. The results showed that the initial pH of the shells has a significant effect on the temperature, carbon...

Author(s): Abdel Ghaly and Nesreen Mahmoud

December 2015

Characterization of gamma-irradiated seeds of a wild Namibian marama bean (Tylosema esculentum) with microsatellite markers

Legumes have been used as food by indigenous people of Africa yet their full potential as food sources has not been completely realized. With modern technologies such as marker-assisted selection and mutational breeding, there is scope for rapid improvement of germplasm in these underutilized species. One approach is to induce genetic mutations through irradiation of plant tissues with neutrons or gamma rays generating...

Author(s): Takundwa, M. M., Ruzvidzo, O., Nambahu, F., Kawadza, D. T., Chatukuta, P. and Chimwamurombe, P. M.

November 2015

In vitro propagation of two elite cooking banana cultivars- FHIA 17 and INJAGI

Explants of two elite cooking banana cultivars, FHIA17 and INJAGI were collected from healthy source of stock plants growing in the field. Sterilization was evaluated using different concentrations (20, 30, 40, 50 and 60%) of a commercial bleach (JIK) for 25 min. Effects of cytokinins benzyl amino purine (BAP), 2-isopentenyl adenine (2iP) and kinetin each evaluated at 5, 10, 20, 24 and 40 µM and thidiazuron (TDZ)...

Author(s): Jane Kahia, Felicien Ndaruhutse, Bancy Waweru, Ntirugulirwa Bonaventure, Amini Mutaganda, Peter Yao Sallah, Njenga Peter Kariuki and Theodore Asiimwe

June 2015

RNA Interference – A fine tuner of gene regulation: a Review

The discovery of RNA interference by Fire and Mello has opened up a new arena for research in molecular biology. The interfering RNAs (iRNAs) are 21-23 nucleotide RNA duplexes, which have the ability to reduce or abolish gene activity, and this property is being used in reverse genetics. Dicer and RISC (RNA-induced silencing complex) play important role in mechanism of RNA interference. The specificity, efficiency and...

Author(s): S.K. Dash, Sushil K. Mohapatra and H.N. Malik

May 2015

Induction and regeneration of somatic embryos from Vitex doniana (Lamiaceae) leaf explants

The present study was conducted with the aim of evaluating some of the factors that influence induction and regeneration of somatic embryos in Vitex doniana since there are no available reports on tissue culture of this tree species. Leaves from plants growing under temporary shed were cultured on Murashige and Skoog supplemented with silver nitrate and four amino acids (proline, tryptophan, lysine and leucine) at...

Author(s): Colombe Dadjo, Jane Kahia, Catherine Muthuri, Lucien Diby, Christophe Kouame, Peter Njenga and Modeste Kouassi

March 2015

Production, purification and characterization of a thermostable alkaline serine protease from Bacillus lichniformis NMS-1

Alkaline proteases are used in food industry, leather tanning and processing industry, preparation of pharmaceuticals and also in the fiber industry. An alkaline serine protease producing strain was isolated using soil sample from a natural hot water spring in Sri Lanka. It was identified based on morphological, biochemical and 16s rRNA identifications as Bacillus licheniformis NMS-1. The extacellular protease enzyme...

Author(s): C. D. Mathew and R. M. S. Gunathilaka

February 2015

Genetic diversity of sesame germplasm collection (SESAMUM INDICUM L.): implication for conservation, improvement and use

Genetic diversity assessment of genetic resources maintained at Gene-Banks has important implication for future improvement, conservation and collection activities. However, such information is not available for sesame collected by IBC, Ethiopia. Inter simple sequence repeat (ISSR) marker was used to assess the level of genetic diversity, genetic structure and genetic distance, and to indirectly estimate the level of...

Author(s): Dagmawi Teshome  Woldesenbet, Kassahun Tesfaye and Endashaw Bekele

January 2015

A new method for the detection of oil degrading genes in Pseudomonas aeruginosa based on transformation and PCR hybridization

Biodegradation is the chemical breakdown of materials by a physiological environment. The term is often used in relation to ecology, waste management and environmental remediation. A specific gene from bacteria is identified and sequenced which has the capacity of oil degradation without any obvious strain specific discrimination using a combination of PCR and hybridization. The parameters of biodegradation that is...

Author(s): Shiju Mathew and Yahya Hasan Hobani

December 2014

Molecular identification of Lactobacillus plantarum isolated from fermenting cereals

The identification of a microbial isolate to genus level only amounts to a partial characterization of the isolate, but this can tell us a lot about that organism. Knowing the species allows the laboratory access to the body of knowledge that exists on that species. Identification schemes using phenotypic characteristics such as colony and cell morphology, Gram reaction and other staining characteristics, nutritional...

Author(s): Adeyemo, S. M. and Onilude, A. A.

December 2014

Molecular characterization of resistance to Russian wheat aphid (Diuraphis noxia Kurdjumov) in bread wheat (Triticum aestivum L.) line KRWA9

The Russian wheat aphid (RWA), Diuraphis noxia (Kurdjumov) causes extensive economic damage to wheat (Triticum aestivum L.) in most wheat growing regions of the world. Control of RWA using systemic insecticides is expensive and pollutes the environment therefore the most effective method of RWA control is the development of RWA resistant cultivars. This study was initiated to determine inheritance of RWA resistance in a...

Author(s): E. A. Masinde, J. N. Malinga, W. A. Ngenya, R.M.S Mulwa and M. Cakir

August 2014

Assessment of different samples for molecular diagnosis of extra-pulmonary tuberculosis

Extrapulmonary tuberculosis is an important clinical problem particularly in developing countries. The aim of this study was to assess two different samples (blood and fluid) for the diagnosis of extra-pulmonary tuberculosis (abdominal tuberculosis and tuberculous lymphadenitis). The study subjects were recruited from WadMedani Teaching Hospital during 2009-2013. Seventy five ascetic fluid and blood samples were...

Author(s): Gasmelseed N., Aljak M. A., Elmadani A. E., Elgaili M. E. and Saeed O. K.

August 2014

Use of the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique to analyse the Anthocyanidin Synthase (ANS) gene Locus in Zimbabwean sorghum landraces with different seed proanthocyanidin profiles

Studies on the effects of mutations within flavonoid pathway genes on the resultant flavonoid profiles in sorghum are important in the identification and characterisation of varieties with nutritionally superior flavonoid profiles. In this study, we aimed at determining the effect of mutations at one important flavonoid pathway locus, the anthocyanidin synthase (ANS) gene, on grain flavonoid profile in sorghum. Sequence...

Author(s): Ngonidzashe Mangoma and Zephaniah Dhlamini

July 2014

Effect of common feed enzymes on nutrient utilization of monogastric animals

Some nutrients in livestock feed may not be fully digested by animals own digestive enzymes, and hence important nutrients are unavailable to the animal. Although, supplementation of enzymes to farm animals has shown to increase the digestibility of poorly digested diets to a much greater extent. By targeting specific anti-nutrients in certain feed ingredients, feed enzymes allow especially pigs and poultry to extract...

Author(s): Bimrew Asmare

July 2014

Biotechnological production of α-amylases for industrial purposes: Do fungi have potential to produce α-amylases?

Enzymes are substances produced by a living organism which acts as a catalyst to bring about a specific biochemical reaction. Amylases are a class of hydrolytic enzymes, widely spread in nature having varied application in different industrial processes and constitute a class of industrial enzymes. Fungal amylases have been widely used for the preparation of oriental foods. In spite of the wide distribution of amylases...

Author(s): Gowhar Hamid Dar, Azra N. Kamili, Ruqeya Nazir, Suhaib A. Bandh and Tauseef Ahmad Malik

June 2014

Anthology of historical development and some research progress glimpses on phytochemical antioxidants phenomenon

The inevitability of applications of antioxidants in protection of human and preservation of dormant living systems such as food, drugs, cosmetics and other allied chemicals against internal and external stresses have made antioxidants an area of active research world over. Study impact of antioxidant research has advanced from topic level to subject level as revealed by the volume of antioxidants research publications....

Author(s): Daramola, B.

May 2014

Effect of humic acid application on accumulation of mineral nutrition and pungency in garlic (Allium sativum L.)

Humic acid promote the conservation of mineral nutritions and as well as stimulate the pungency usually being more prominent in the cloves of garlic bulb. The effect of humic acid application on accumulation of mineral nutritions and pungency in garlic (Allium sativum L.) cv. ‘Gangajali’ was studied. Here, we observed that the maximum concentration of Ca, Fe and S were shown when plants were treated with 400...

Author(s): Manas Denre, , Soumya Ghanti and Kheyali Sarkar

April 2014

In vitro effects of gibberellic acid and sucrose concentration on micropropagation of two elite sweet potato cultivars in Rwanda

The current study aimed at evaluating the effect of gibberellic acid (GA3) and sucrose on in vitro propagation of two elite sweet potato cultivars (Ukerewe and Gihingamukungu). Nodal explants from in vitro growing plantlets were harvested and cultured on Murashige and Skoog media supplemented with 2.5, 5, 10, 20 and 40 μM, GA3. In a separate experiment, sucrose was evaluated at 30, 60, 90, 120, 150, 180 and 210 mM....

Author(s): Valery Ndagijimana, Jane Kahia, Theodore Asiimwe, Peter Yao Sallah, Bancy Waweru, Isidore Mushimiyimana, Jean Ndirigwe, Sindi Kirimi, Damien Shumbusha, Peter Njenga, Modeste Kouassi and Edmond Koffi

December 2013

Influence of preservative solutions on vase life and postharvest characteristics of rose (Rosa hybrid) cut flowers

The experiment was carried out to asses the influence of five preservative solutions (aluminium + ethanol, aluminium + sucrose, ethnol + sucrose, aluminium + ethanol + sucrose and water) and two rose cultivars (‘Red Sky’ and ‘Blizzard’). The scope of the study was to identify the best combination of preservative solutions on rose cultivars. The treatments were arranged in factorial combination in...

Author(s): Hailay Gebremedhin, Bizuayehu Tesfaye, Ali Mohammed and Dargie Tsegay

December 2013

Genetic variation of 17 Y-chromosomal short tandem repeats (STRs) loci from unrelated individuals in Iraq

Analysis of 17 Y chromosomal STR loci in a population sample of 400 unrelated male living in the middle and south of Iraq was done to evaluate allele frequencies and gene diversity for each Y-STR locus of the Y filer™ PCR amplification kit. The seventeen loci include DYS635, DYS437, DYS448, DYS456, DYS458, YGATA H4, DYS389I, DYS389II, DYS19, DYS391, DYS438, DYS390, DYS439, DYS392, DYS393, DYS385a and DYS385b....

Author(s): Imad H., Cheah Q., Mohammad J. and Aamera O.

November 2013

Effects of harvesting stage and storage duration on postharvest quality and shelf life of sweet bell pepper (Capsicum annuum L.) varieties under passive refrigeration system

A laboratory experiment was carried out to determine the effects of harvesting stages (0, 25, 50, 75 and 100% fruit colourations) and storage durations (0, 1, 2, 3 and 4 weeks) on physicochemical quality and shelf life of sweet pepper varieties (Telmo-Red and Velez-Yellow) under passive refrigeration system (PRS). The aim of the study was to identify the optimum stage of maturity at harvest and storage period under PRS...

Author(s): Dargie Tsegay, Bizuayehu Tesfaye, Ali Mohammed, Haddis Yirga and Andnet Bayleyegn

November 2013

Diversity analysis of sugarcane genotypes by microsatellite (SSR) markers

Thirty (30) simple sequence repeat (SSR) primer pairs chosen randomly from the SSR primer collection were used to detect polymorphism in 17 sugarcane accessions. A total of 62 DNA fragments were generated by the 30 primers with an average of about 2.14 bands per primer. Bands that a primer yielded in the study ranged from 1 to 4. The genetic distances for SSR data using 17 sugarcane accessions, was constructed based on...

Author(s): Smiullah, Farooq Ahmed Khan, Aqeel Afzal, Abdullah, Ambreen Ijaz and Usman Ijaz

October 2013

Micropropagation of the Indian Birthwort Arsitolochia indica L.

Aristolochia indica L. is a medicinal woody perennial climber plant of immense pharmaceutical value. The species is endangered with possible extinction due to its indiscriminate harvesting as raw material for pharmaceutical industry, to manufacture drugs against cholera, inflammation, biliousness, dry cough and snake bite. A rigorous attempt has been made for development of in vitro propagation...

Author(s): Syed Naseer Shah, Amjad M. Husaini and Fatima Shirin

October 2013

A quick bud breaking response of a surface model for rapid clonal propagation in Centella asiatica (L.)

Present investigation was planned to evaluate time period of bud breaking in Centella asiatica with different concentration of plant growth regulators, a medicinal herb distributed throughout the worldwide. For the study, concentrations were designed for response surface model describing bud breaking growth in optimum conditions. A combination of BAP (2 mg/L) + gibberellic acid (GA3, 0.5 mg/L) was...

Author(s): AK Bhandari, M Baunthiyal, VK Bisht, Narayan Singh and JS Negi

September 2013

Molecular characterisation of two cichlids populations (Tilapia guineensis and Sarotherodon melanotheron) from different water bodies in Lagos State, Nigeria

  This study used random amplified polymerase DNA technique (RAPD) to characterize two populations of cichlids species Tilapia guineensis and Sarotherodon melanotheron from selected two water bodies in Lagos using molecular approach. Ten samples of each S. melanotheron and T. guineensis with standard length value ranging between 8.4 and 17.2 cm and their...

Author(s):   B. A. Usman, O. T. Agbebi, M. O. Bankole, O. R. Oguntade and M. O. Popoola

September 2013

Micropropagation of Litsea glutinosa (Lour) C.B

    Litsea glutinosa (Lour) C.B (Hindi: Maida lakri) is a medicinal plant of immense pharmaceutical value. The species is critically endangered due to its indiscriminate collection as raw material for pharmaceutical industry, where it is used for manufacturing drugs for pain, arousing sexual power and in treatment of diarrhea and dysentery etc. An attempt has been made for development of in vitro...

Author(s): Syed Naseer Shah, Amjad M. Husaini and S. A. Ansari

August 2013

Cell growth curves for different cell lines and their relationship with biological activities

  The cellular-growth curves for distinct cell lines (tumor and non-tumor) were established, evaluating the population of doubling time (DT) and maximum growth rate (µmax). These curves define the growth characteristics for each cell line; they allow determination of the best time range for evaluating the effects of some biological compounds. Subsequently, the biological activities of three ethanolic...

Author(s): Iloki Assanga, S. B., Gil-Salido, A. A., Lewis Luján, L. M., Rosas-Durazo, A., Acosta-Silva, A. L., Rivera-Castañeda, E. G. and Rubio-Pino, J. L.

August 2013

Beta glucuronidase activity in early stages of rice seedlings and callus: A comparison with Escherichia coli beta glucuronidase expressed in the transgenic rice

We have chosen rice as a model crop to ascertain endogenous β-glucuronidase (family 79) activity and to differentiate the same from Escherichia coli based β-glucuronidase (family-2). The investigation dwells on characterizing endogenous β-glucuronidase (GUS) activity during the early stages of seed germination and from rice callus. Also, similar studies were made from homozygous transgenic...

Author(s): Poosakkannu Anbu, and Loganathan Arul

June 2013

Use of SSR markers for genetic diversity studies in mulberry accessions grown in Kenya

The knowledge and understanding of the extent of genetic variation of mulberry germplasm is important for conservation and improvement. The objective of this study was to analyze the genetic diversity between and within two mulberry species widely grown in Kenya which include Morus alba and Morus indica. Five individuals per species were genotyped with 13 simple sequence repeat (SSR) markers. The SSR markers...

Author(s): Nderitu Peris Wangari, Kinyua Miriam Gacheri, Mutui Mwendwa Theophilus and Ngode Lucas

June 2013

Assessment of antifungal effect of omeprazole on Candida Albicans

The goal of this study was to investigate the in vitro effect of  omeprazole on Candida albicans and analyze the antifungal activity of omeprazole. A total of 150 samples were collected from the patients in Bakirköy Dr. Sadi Konuk Education and Research Hospital and samples were evaluated for C. albicans. After the microbiological analyses, fifty one patients (18 men and 33...

Author(s): Fahriye Küçükaslan, Hasibe Cingilli Vural, Didem Berber, Zeki Severoğlu, Sabri Sümer and Meltem Doykun

May 2013

Influence of biofertilizer on essential oil, harvest index and productivity effort of black cumin (Nigella sativa L.)

In order to investigate the effects of biofertilizer on essential oil harvest index and productivity effort of black cumin (Nigella sativa L), an experiment was conducted during the growing season of 2010 at Iran. The experimental design was factorial on the basis of randomized complete block design with three replications. Certain factors including four levels of animal manure (0, 10, 20 and 30 ton/ha, respectively)...

Author(s): Seyed Alireza Valadabadi and Hossein Aliabadi Farahani

May 2013

Large scale recovery of tetanus toxin and toxoid from fermentation broth by microporous tangential flow filtration

The commercial production of purified tetanus toxoid mainly depends on the effective separation of the bacterial toxin and toxoid from large volumes of fermentation broth of Clostridium tetani (Harvard 49205) vaccine strain. Tangential flow or cross-flow filtration system was used as rapid drive in the processing of immunobiological assays of tetanus toxin. Tetanus toxoid was prepared by detoxifying the...

Author(s): Chellamani Muniandi, Kavaratty Raju Mani and Rathinasamy Subashkumar

March 2013

Development of cost-effective media for the culture of Chilo partellus larvicide in Kenya

Stem borers (Chilo partellus) are important field insect pests of maize (Zea mays L.) and sorghum (Sorghum bicolor L.) in Africa. They account for more than 30% yield losses depending on the composition of the pest community. C. partellus larvicide like Bacillus thuringiensis have been widely and effectively used in C. partelluscontrol programs, but the industrial production of...

Author(s): Daniel Anyika, Hamadi Boga and Romano Mwirichia

March 2013

Molecular cloning, expression, sequence analysis and in silico comparative mapping of trehalose 6-phosphate gene from Egyptian durum wheat

Trehalose is a non-reducing disaccharide which consists of two glucose units that functions as a compatible solute to stabilize the membrane structures under heat and desiccation stress. Trehalose-6-phosphate synthase (TPS) and trehalose-6- phosphate phosphatase (TPP) are the key enzymes for trehalose biosynthesize in the plant kingdom. On the basis of bioinformatics prediction, fragment containing an open reading frame...

Author(s): Ayman A. Diab,  Ahmed M. K. Nada and Ahmed Ashoub

October 2012

Gene diversity and identification of putative hybridizing parents for root rot resistance in cassava using simple sequence repeats

The incidence of root rot diseases partly contribute to the currently observed low percentage increase in the yield of cassava. We estimated gene diversities and identified putative hybridizing parents for root rot resistance using 18 simple sequence repeats loci in 43 improved genotypes of cassava. Root rot was measured over 2 years as the percentage proportion of rotten roots to the total number of roots harvested at...

Author(s): Oluwasayo Kehinde Moyib,, Jonathan Mkumbira, Oyeronke Adunni Odunola and Alfred Godwin Dixon,

October 2012

Effect of feeding malted foods on the nutritional status of pregnant women, lactating women and preschool children in Lepakshi Mandal of Ananthapur district, Andhra Pradesh, India

Information of preschool children (400), pregnant women (100) and lactating women (100) was collected.  Anthropometric, hemoglobin, clinical and morbidity assessments were carried out before & after supplementation.  Amylase Rich Malted Mixes (ARMMs) 2 types (Ragi/Wheat) were formulated and suitable products namely laddu, roti, kheer and porridge were prepared using formulated malted...

Author(s): Vijaya Khader and K. Uma Maheswari

June 2012

Enhancing thermostability of the biocatalysts beyond their natural function via protein engineering

Majority of the naturally occurring enzymes lacks essential features required during the harsh conditions of the industrial processes, because of their less stability. Protein engineering tool offers excellent opportunity to improve the biochemical properties of these biocatalysts. These techniques further help in understanding the structure and function of the proteins. Most common methods employed in protein...

Author(s): Shelly Goomber, Pushpender K. Sharma, Monika Sharma, Ranvir Singh and Jagdeep Kaur

June 2012

Extraction, purification and characterization of L-asparaginase from Penicillium sp. by submerged fermentation

A fungal strain identified as Penicillium sp. was evaluated for its L–asparaginase enzyme production. The L-asparaginase enzyme was purified to homogeneity from Penicillium sp. that was grown on submerged fermentation. Different purification steps including salt precipitation, followed by separation on sephadex G-100-120 gel filtration and DEAE were applied to obtain pure enzyme preparation....

Author(s): Krishna Raju Patro and Nibha Gupta

March 2012

Chromium stress on peroxidase, ascorbate peroxidase and acid invertase in pea (Pisum sativum L.) seedling

In this investigation, chromium (Cr) toxicity on enzymes like peroxidase, ascorbate peroxidase and acid invertase was studied in pea seedling to evaluate the relative tolerance of the different pea varieties. Effect of Cr (0, 1.0, 2.0, 3.0 mM) on different parts of the seedling that is, cotyledon, plumule and radicle were studied separately to have a comparative study of the enzyme activity in these parts of the plants...

Author(s): Surekha and Joginder Singh Duhan

March 2012

Histopathological studies of microsporidian infected tissues of Lamerin breed of the silkworm, Bombyx mori L.

Ultrastructure of gut and fat bodies of Lamerin breed of the silkworm Bombyx mori L infected with microsporidia did not show hypertropy of cells but structural disorganization was obvious in infected tissues. The different developmental stages (meronts and sporonts) and a few mature spore could be observed in direct contact with cell cytoplasm. Key words: Bombyx...

Author(s): Shabir A. Bhat, B. Nataraju and Ifat Bashir

January 2012

Studies of wine produced from pineapple (Ananas comosus)

Pineapple (Ananas comosus) is the common name for a tropical plant with edible fruit, which is actually a multiple fruit, consisting of coalesced berries. The ratio of 1: 4 (pineapple must: sugar) was used to produce wine using recipes A to D. A contained only natural yeast; B contained natural yeast augmented with granulated sugar; C contained natural yeast augmented with baker’s yeast and granulated sugar...

Author(s): Idise, Okiemute Emmanuel

January 2012

A survey of avian malaria parasite in Kano State, Northern Nigeria

As an attempt to keep abreast of the variety of avian Plasmodium parasite in Kano State, a total of 218 blood films were made from wild (116) and domesticated (102) bird species collected between January and July, 2009 period. The slides were examined for the presence of Plasmodium parasite (parasitaemia value). Birds examined were six Columbidae livia (pigeons), two Cisticola...

Author(s): Karamba K. I, Kawo A. H., Dabo N. T and Mukhtar M. D

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