African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007

AJBR Articles

August 2010

Corncob is the optimum carbon resource for the growth of Tremella aurantialba

The minimum medium without carbon source was used to investigate the effects of five kinds of hexoses, four kinds of pentose, four kinds of bi-saccharide and three kinds of polysaccharides on Tremella aurantialba growth. The result demonstrated that (1) The fittest carbon source of T. aurantialba was the five-carbon aldose in all the tested monosaccharides; (2) Polysaccharides linked with...

Author(s): Zhi-Cai Zhang, Mingxia Chen, Xin Li and Wangli Shen

  • Article Number: 8690E2B11535

August 2010

Metabolic engineering of an ethanol-tolerant Escherichia coli MG1655 for enhanced ethanol production from xylose and glucose

Efficient ethanol production will require a recombinant to able to ferment a variety of sugars (pentoses, and hexoses), less formation of by-products, as well as to tolerate high ethanol stress. In this study, a mutant (MGE) that can grow in 60 g ethanol/l was selected from Escherichia coli MG1655 by enrichment method with increasing concentrations of ethanol. The ethanol-tolerant mutant was used as the host...

Author(s): Ruiqiang Ma, Ying Zhang, Haozhou Hong, Wei Lu, Wei Zhang Min Lin and Ming Chen

  • Article Number: ECF1BFD11537

July 2010

Evaluation of nutritional and antinutritional characteristics of Obeche (Triplochition scleroxylon scleroxylon) and several Mulberry (Morus alba) leaves

The biochemical composition of some selected varieties of White Mulberry and Obeche leaves were investigated to ascertain their nutritional and antinutritional values. Three varieties of White Mulberry leaves (S36, S54 and K2) were harvested from Ondo State sericulture centre while Obeche leaves were harvested from Aponmu Forest Reserve located in Ondo State, Southwestern Nigeria, for analysis. Proximate chemical...

Author(s): S. A. Adeduntan and A. S. Oyerinde

  • Article Number: CB3010911484

July 2010

Phytochemical screening and histopathological effects of single acute dose administration of Artemisia annua L. on testes and ovaries of Wistar rats

Artemisia annua L. is a medicinal plant whose derivatives are used in the treatment of malaria. Phytochemical screening and histopathological effects of single acute intraperitoneal dose of the alcoholic extract of A. annua, were investigated in Wistar rats in this study. Thirty five adult albino Wistar rats (109 - 307 g) of both sexes used for the study were randomly assigned on the basis of weight and...

Author(s): P. O. Ajah and M. U. Eteng

  • Article Number: F1CD96411505

July 2010

Standardized alcoholic extract of Phyllanthus fraternus exerts potential action against disturbed biochemical parameters in diabetic animals

Alcoholic extract of Phyllanthus fraternus Webster whole plant (PFAE) prepared by successive solvent treatment was administered at a dose of 500 mg/kg body weight once in a day for 21 days to the alloxan induced diabetic albino rats. Certain biochemical parameters that is lipid profile (total cholesterol, high density lipoprotein, triacylglycerols), kidney functions (urea, creatinine) and liver functions...

Author(s): Munish Garg, Chanchal Garg, V. J. Dhar and A. N. Kalia

  • Article Number: 4402F3611513

July 2010

Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo

The present study was carried out for identification of the phytochemicals present in the Vitex negundo leaves and also evaluate the total phenols, total flavonoids and antioxidant activity of the leaf extract. Total phenols was carried out by Folin Ciocalteu method and the phenolic content was 27.72 mg/100 of gallic acid equivalent (GE). Antioxidant activity was evaluated by DPPH method and the leaves...

Author(s): P. Praveen Kumar, S. Kumaravel and C. Lalitha

  • Article Number: 743A94611519

June 2010

Effects of diet-induced hypercholesterolemia on the lipid profile and some enzyme activities in female Wistar rats

The effects of a high dietary soybean oil and cholesterol on serum total cholesterol, low density lipoprotein (LDL-C), high density lipoprotein (HDL-C), and triglycerides were investigated. Total protein, albumin, glucose, gamma glutamyl transferase (GGT), aspartate aminotransferase (AST) and alanine amino transferase (ALT) activities were also investigated in weanling female Wistar rats for eight weeks. Two groups of...

Author(s): Gloria A. Otunola, Oyelola B. Oloyede, Adenike T. Oladiji and Anthony A. Afolayan

  • Article Number: 5D7026911395

June 2010

Malate dehydrogenase from blood stream Trypanosoma vivax exists in isoenzyme forms

Malate dehydrogenase was isolated and partially purified from blood streamTrypanosoma vivax. The enzyme exists in isoenzyme forms (MDH1 and MDH2). The relative molecular weights of MDH1 and MDH2 determined by Sodium dodecyl sulphate polyacrylamide gel electrophoresis were 61 and 63 kdal. respectively. MDH2 lost activity immediately after purification. It has purification fold of 15 and percentage...

Author(s): A. U. Wurochekke, A. J. Nok, H. M. Inuwa and C. E. Gimba

  • Article Number: 2EAA93511418

June 2010

Purification and characterization of the α-amylase isolated from Penicillium camemberti PL21

The amylase family of enzymes is a great significance due to its wide area of potential application. α-amylase from Penicillium camemberti PL21 obtained from I. N. A. (The technological laboratory of Agriculture National Institute Paris- France) and using orange waste as substrates was produced under optimum conditions, after 168 h of incubation and subjected for purification and characterization. The...

Author(s): Tahar Nouadri, Zahia Meraihi, Djekrif-Dakhmouche Shahrazed and Bennamoun Leila

  • Article Number: 3B4802A11438

June 2010

The distribution of cyanide detoxifying enzymes (rhodanese and 3- mercaptopyruvate sulphurtransferase) in different species of the family Cichlidae (Tilapia zillii, Sarotherodon galilaeus and Oreochromis niloticus)

The cyanide metabolising enzymes (rhodanese and mercaptopyruvate sulphurtransferase) were estimated in different tissues of three species of tilapia (Tilapia zilli, Sarotherodon galileus,and Oreochromis niloticus) from two locations (Aiba and Osinmo reservoirs, both in the South-Western region of Nigeria). The enzyme activities were carried out by measuring the amount of thiocyanate produced by the two enzymes...

Author(s): Okonji Raphael Emuebie, Popoola Michael Olaoluwa, Kuku Adenike and Aladesanmi Omolara Titilayo

  • Article Number: B5C8B6211451

June 2010

Characterization of antioxidant and antimicrobial compounds of cinnamon and ginger essential oils

Essential oils obtained from the bark of Cinnamomum zeylanicum (cinnamon) and the rhizomes of Zingiber officinale (ginger) were characterized by analytical TLC and GC/MS, and their antimicrobial and antioxidant compounds were detected by TLC-bio-autography assays. Essential oil of cinnamon bark (CEO) was found to be a unique aromatic monoterpene-rich natural source, with trans-cinnamaldehyde...

Author(s): G. S. El-Baroty, H. H Abd El-Baky, R. S. Farag and M. A. Saleh

  • Article Number: 8D4DEA111465

May 2010

Production of yeast using acid-hydrolyzed cassava and poultry manure extract

Cassava is made up of starch as its major nutritive reserve. Starch which is one of the most important products synthesized by plants is consumed as food and can be used in industrial processes. This investigation seeks to explore the availability of cassava as a source of glucose as well as poultry manure as a source of nitrogen in the production of yeast. Cassava flour was hydrolyzed with 0.5% (v/v) concentrated...

Author(s): L. I. Osumah and N. J. Tonukari

  • Article Number: AFEA22E11312

May 2010

AQY1 gene from Debaryomyces hansenii

Aquaporins are members of the major intrinsic protein superfamily of integral membrane proteins that enable the transport of water, glycerol and other solutes across membranes in diverse organisms. In yeasts, the proposed physiological roles of aquaporins are related to their contribution to freeze tolerance, osmoregulation and water transport. This article reports a contribution to the freeze tolerance in the...

Author(s): Juan Carlos González-Hernández

  • Article Number: 56A587A11332

May 2010

Ginger extract (Zingiber officinale) triggers apoptosis and G0/G1 cells arrest in HCT 116 and HT 29 colon cancer cell lines

Although many studies have shown the antitumor properties of ginger extract (Zingiber officinale), little is known regarding the mechanism of its effects. This study was conducted to determine the mechanism of antitumor effects of ginger extract by evaluating apoptosis rate and cell cycle progression status in colon cancer cell lines HCT 116 and p53 defective HT 29. HCT 116 and HT 29 cells were cultured in the presence...

Author(s): Shailah Abdullah, Siti Amalina Zainal Abidin, Noor Azian Murad, Suzana Makpol, Wan Zurinah Wan Ngah and Yasmin Anum Mohd Yusof

  • Article Number: A5B217C11349

April 2010

Application of acid-hydrolyzed cassava (Manihot esculenta) and cowpea (Vigna unguiculata) for the production of yeast (Saccharomyces cerevisiae)

Much progress has been made in the cultivation and production of cassava (Manihot esculenta) and cowpea (Vigna unguiculata) in Nigeria. In the present study, investigation was carried out on the possibility of using cassava flour as a source of glucose as well as cowpea as source of nitrogen in the production of yeast. Acid hydrolysis (using dilute H2SO4) of cassava and cowpea was undertaken to release the sugars and...

Author(s): Nyerhovwo J. Tonukari and Linda I. Osumah

  • Article Number: E6992CB11198

April 2010

Captopril interferes with some serum biochemical findings

Captopril is a widely used anti-hypertensive drug that acts by inhibiting angiotensin-converting enzyme. This work has been carried out to investigate the effects of captopril on some common biochemical laboratory parametres in the sera of patients receiving the drug. For this study, 40 subjects were included, all within the age range of 40 to 63 years and with newly diagnosed essential hypertension. From each patient,...

Author(s): I. A. Ibrahim, F. S. Al-Joudi , R.  Waleed Sulaiman  and B. Hilal AL-Saffar

  • Article Number: EC757BD11233

April 2010

Effect of ferric oxide nanoparticles on microtubules organization

Nanoparticles (NPs) are widely used in several manufactured products. The small size of nanoparticles facilitates­ their uptake into cells as well as transcytosis across epithelial cells into blood and lymph circulation to reach different sites, such as the central nervous system. Studies have shown different risks of Fe2O3NPs in the neuronal system and other organs. They are membrane-bound layer aggregates or...

Author(s): Ali Khaleghian, Gholam Hossein Riazi, Shahin Ahmadian, Mahmoud Ghafari, Marzieh Rezaie, Akira Takahashi, Yutaka Nakaya and Hossein Nazari

  • Article Number: 1555A4311254

April 2010

Synergistic effects of glucan and resveratrol

Recent data showing that glucan stimulates defense reactions in plants through synthesis of resveratrol, led us to study the possible synergetic effects of a glucan-resveratrol complex on immune reactions in mice. We measured phagocytic activity, expression of CD4 marker on spleen cells, IL-2 secretion and antibody response. In all cases we confirmed the stimulatory effects of glucan. Resveratrol alone had either limit...

Author(s): Vaclav Vetvicka and Zuzana Vancikova

  • Article Number: D4B8D1E11261

April 2010

Assessing the role of RecA protein in the radioresistant bacterium, Deinococcus geothermalis

The moderately thermophilic bacterium Deinococcus geothermalis exhibits extraordinary resistance to ionizing radiation. RecA protein is considered to be one of the most important participants in radioresistance. To assess the role of the RecA protein in D. geothermalis, the recA gene was isolated from D. geothermalisand over expressed in Escherichia coli. After the D. geothermalis RecA...

Author(s): Haïtham Sghaier, Katsuya Satoh, , Hirofumi Ohba,  and Issay Narumi,

  • Article Number: 4122D0C11282

March 2010

Fermentation in cassava (Manihot esculenta Crantz) pulp juice improves nutritive value of cassava peel

A major challenge in using cassava peel as feed for animals is the presence of cyanogenic glycosides and the low concentration of protein. The present study investigated the possibility of upgrading cassava peels using fermented cassava pulp juice. Cassava pulp juice was squeezed out of grated cassava pulp and fermented for 3 days at ambient temperature. The microorganisms in the fermented pulp juice were identified...

Author(s): Naa Ayikailey Adamafio, Maxwell Sakyiamah and Josephyne Tettey

  • Article Number: 8AB81A811059

March 2010

Production of cuticle - degrading proteases by Beauveria bassiana and their induction in different media

The concern for the development of hyphomycete fungi as suitable biocontrol agents of insect pests leads to the isolation of various insect pathogenic fungi. Amongst them, one of the most studied entomopathogenic fungus is Beauveria bassiana. The conidia of mitosporic fungi adhere to the host cuticle and germinate to produce an infectious propagule, and produce a sequential release of extra cellular enzymes to...

Author(s): Priyanka Dhar and Gurvinder Kaur

  • Article Number: 3E2873A11120

March 2010

Optimization of culture parameters to enhance production of amylase and protease from Aspergillus awamori in a single fermentation

Concomitant production of amylase and protease by Aspergillus awamorinakazawa MTCC 6652 was enhanced in a single fermentation by media engineering and optimization of other important parameters. Wheat bran was considered as a suitable substrate for production of amylase and protease by A. awamori in a single fermentation as none of the additional substrate such as powder of peanut, corn, soybean seeds and...

Author(s): Sangeeta Negi and Rintu Banerjee

  • Article Number: E2314C711148

March 2010

Hepatoprotective role of Garcinia kolanut (Heckel) extract on methamphetamine: Induced neurotoxicity in mice

The hepatoprotective effect of aqueous extract of Garcinia kola (AEGK) was studied in 60 mice of mixed sexes. The animals were divided into 6 groups of 10 mice each. Group I received normal saline, Groups II and III got 100 and 200 mg/kg AEGK (orally), respectively. Group IV received 10 mg/kg methamphetamine (MAM) (s.c.) only. Groups V and VI got 100 and 200 mg/kg of AEGK respectively, before 10 mg/kg methamphetamine...

Author(s): Gabriel Oze, Iheanyi Okoro, Austin Obi and Polycarp Nwoha

  • Article Number: 8BCCC2211162

February 2010

Lecithin: Cholesterol acyltransferase, lipoprotein lipase and lipoproteins in adult Nigerians with sickle cell disease

Lecithin: Cholesterol acyltransferase (LCAT) is a glycoprotein enzyme involves in the esterification of plasma cholesterol to form cholesteryl ester and is important in the maturation of high density lipoprotein. Lipid metabolism in sickle cell anaemia patients may be affected. The objective of this study was to determine the activities of serum lecithin: cholesterol acyltransferase, lipoprotein lipase, lipids and...

Author(s): M. A. Emokpae, , P. O. Uadia and H. B. Osadolor

  • Article Number: 8F62AF710949

February 2010

Toxic effect of lead on kidney function in rat wistar

Lead is one of the environmental pollutants that can threat the life of living creatures in many ways. This study was carried out to investigate the effect of lead exposure on kidney function. Fourteen male Wistar rats were divided into 2 groups; group 1 was given a tap water diet and group 2 was given 1000 ppm lead acetate in drinking water for 8 weeks. Lead concentration in blood was determined by atomic absorption....

Author(s): Missoun F, Slimani M and Aoues A

  • Article Number: 547955B10974

February 2010

Changes in haemorrheologic and fibrinolytic activities upon hypertension and diabetic chemotherapy in Calabar diabetic residents, Nigeria

This research was carried out to assess the effect of hypertension on haemorrheologic and fibrinolytic activities in fifty (50) diabetics resident in Calabar municipality and the values obtained were compared with those of fifty (50) age and sex-matched non diabetics in the same locality. Relative plasma viscosity plasma fibrinogen concentration, euglobulin lysis time and fasting blood sugar were determined using...

Author(s): M. S. Edem, A. O. Emeribe and J. O. Akpotuzor

  • Article Number: 3D56F8910989

February 2010

The response to iron deficiency of two sensitive grapevine cultivars grafted on a tolerant rootstock

Two sensitive cultivars were examined, Vittoria and Italia, grafted on a tolerant rootstock (140 Ruggeri). Two levels of iron chlorosis in scions were selected, initial and evident, and compared to the healthy rootstock (control). The fractions of extracellular and cytoplasmatic cations, chloroplastic mobile, loosely linked, strongly linked and residual cations as well as the active fraction were...

Author(s): M. A. Russo, F. Sambuco and A. Belligno

  • Article Number: 2A6DF6E11002

February 2010

The effects of aqueous extracts of the leaves of Hibiscus rosa-sinensis Linn on renal function in hypertensive rats

The leaves of Hibiscus rosa-sinensis Linn (Family Malvaceae) have been used in ethnomedicine for the treatment of various human diseases such as aphrodisiac, hypertension, wound healing, diabetes mellitus and cancer. In this present study, the effect of 200 mg/kg of the aqueous leaf extract on the renal function of hypertensive rats was investigated. The administration of H. rosa-sinensis leaves...

Author(s): Imafidon E. Kate and Okunrobo O. Lucky

  • Article Number: 3CD2F8211018

February 2010

Effects of soaking and boiling and autoclaving on nutritional quality of Mucuna flagellipes (Ukpo)

The effect of soaking and boiling, and autoclaving on nutritional factors (proximate and mineral) and anti-nutritional qualities of legume, Mucuna flagellipes were studied. Batches of seeds were soaked for 6, 12, 18 and 24 h in distilled water at room temperature, then boiled in water for 30, 45, 60 or 90 min respectively. Another batch of the M. flagellipes was autoclaved for different duration of...

Author(s): E. A. Udensi, N. U. Arisa and E. Ikpa

  • Article Number: 6CD16C811036

February 2010

The influence of 950 MHz magnetic field (mobile phone radiation) on sex organ and adrenal functions of male rabbits

Mobile phones are used frequently and held close to the body thus they are potentially dangerous sources of electromagnetic (EM) radiations. The clear effects of EMF (electromagnetic field) on many organs of the body have not been known until now and have puzzled scientists. The objective of this study was the evaluation of the influence of different powers (3 and 6 W) of 950 MHz magnetic field on testosterone, FSH...

Author(s): M. R. Sarookhani, M. Asiabanha Rezaei, A. Safari, V. Zaroushani and M. Ziaeiha

  • Article Number: 898A44712184

January 2010

Effect of egusi melon oil on lecithin: Cholesterol acyltransferase activity in rats fed a cholesterol diet

The effect of feeding egusi melon oil as a supplement to a cholesterol-based diet on serum lecithin: cholesterol acyltransferase activity of rats was evaluated. The rats were divided into two groups designated: control and test respectively. Rats in the test group were fed 5% cholesterol diet supplemented with 5% egusi melon oil while the control rats received 5% cholesterol diet...

Author(s): G. C. Ojieh, G. O. Eidangbe and O. M. Oluba

  • Article Number: C1A9DB910854

January 2010

Assessment of current iodine status of pregnant women in a suburban area of Imo State Nigeria, twelve years after universal salt iodization

Three hundred and two pregnant women participated in this study. Our results showed that the range, mean and median urinary iodine excretion (UIE) were 28.1 to 218.1, 152.09 ± 41.65 and 163.1 µg/l, respectively. The range, mean and median TSH concentration were 0.7 - 5.9, 1.4 ± 0.7 and 1.3 µIU/ml, respectively. Our results showed that none of the women have severe iodine deficiency, 2% had...

Author(s): Cosmas O. Ujowundu, Agwu I. Ukoha, Comfort N. Agha, Ngwu Nwachukwu and Kalu O. Igwe

  • Article Number: 863C90A10886

January 2010

A study on prostate specific antigen (PSA) with the ratio of free to total PSA

Prostate Specific Antigen (PSA) is a glycoprotein produced as a marker by prostate gland for prostate cancer, benign prostate hyperplasia and prostatitis. Study objective is to evaluate f/t PSA ratio to distinguish the Benign Prostate Hyperplasia (BPH) and prostate cancer patients in and around SRM University. To define the age specific reference ranges of PSA in control and test group at Chennai, India. Healthy men...

Author(s): Chavan Atish, Suman Diggi Prasad, Rawal Mukesh, Kandasamy Surendran, Sivapatham Sundaresan, Thangarajan Thangapannerselvem

  • Article Number: 5E4EF1210899

January 2010

Cytoplasmic, peroxisomal and mitochondrial membrane phospholipid alteration in 31-diamino-azo-benzene (31-DAB)-induced hepatocellular carcinoma

Alteration in the cytoplasmic, mitochondrial and peroxisomal membrane phospholipid has been investigated in 31- diamino-azo-benzene (DAB) -induced hepatocellular carcinoma in rats. The cytoplasmic membrane of tumour group shows 27.5, 19.7, 38.7 and 34.7% increase in phosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin respectively compared with the control group, mitochondrial membrane...

Author(s): Omotuyi I. O., Ologundudu A., Okugbo T. O., Oluyemi K. A. and Omitade, O. E.

  • Article Number: 7FEE83012077

January 2010

Investigation of antimicrobial and plasma coagulation property of some molluscan ink extracts: Gastropods and cephalopods

The ink secretion of molluscan species was identified as one of the novel sources of bioactive compounds. Aqueous ink extracts of Dollabella auricularia inhibited the growth of Gram-positive and Gram-negative bacteria. In addition to D. auricularia, Octopus vulgaris and Sepia aculeate ink samples showed antifungal activity. The antimicrobial activities of the samples were not due to the...

Author(s): R. Vennila, R. K. Rajesh kumar, S. Kanchana, M. Arumugam and T. Balasubramanian

  • Article Number: 9E95B4E12094

December 2009

Anti-inflammatory and anti-ulcerogenic activity of the ethanol extract of ginger (Zingiber officinale)

  The acute toxicity test carried out on the ginger extract gave the LD50 value as 1000 mg/kg. The antiinflammatory and anti-ulcerogenic effects of the ethanol extract of ginger (Zingiber officinale) in adult Wistar rats were studied using values below the lethal dose. Inflammation was induced by injecting 0.1 ml undiluted fresh egg albumin (philogistic agent) into the subplantar surface of the...

Author(s): Chioma A. Anosike, Onyechi Obidoa, Lawrence U. S. Ezeanyika and Meshach M. Nwuba.

  • Article Number: 3FC7A2940810

December 2009

Modulation of melanin synthesis and its gene expression in skin melanocytes by palm tocotrienol rich fraction

  Melanin is the pigment that determines skin color. Melanin synthesis is catalysed by the enzyme tyrosinase and is controlled by TYR, TYRP1 and TYRP2 genes. The objective of this study was to evaluate the anti pigmentation property of palm tocotrienol rich fraction by determining melanin synthesis and expression of genes involved in its regulation in skin melanocytes. Palm tocotrienol...

Author(s):   Suzana Makpol, Nur Nadia Mohd Arifin, Zahariah Ismail, Chua Kien Hui, Yasmin Anum Mohd Yusof and Wan Zurinah Wan Ngah   

  • Article Number: 5C41BB540817

December 2009

Antifeedant and antimicrobial activity of Tylophora indica

  Crude and pure extracts of Tylophora indica were investigated in view of antifeedant, antibacterial and antifungal properties.  Leaf crude extract showed more antifeedant activity than stem and root against Spodoptera litura, a polyphagous pest on wide ranging crops. Among the pure compounds isolated, Tylophorine showed the highest antifeedant activity followed by Septicine, O-Methyl...

Author(s):   B. Krishna Reddy, M. Balaji, P. Uma Reddy, G. Sailaja, K. Vaidyanath and G. Narasimha

  • Article Number: E94389640821

November 2009

Effect of falciparum malaria on some plasma proteins in males: With special reference to the levels of testosterone and cortisol

  Sex-associated hormones were evidenced to modulate immune responses and consequently directly influence the outcome of infection. Testosterone is known to influence both protein metabolism and the level of cortisol which is the hormone of stress. This work was conducted to explore the influence of the degree of parasitemia in Plasmodium falciparum malaria on the male sex hormone testosterone, plasma...

Author(s): Muawia A. Abdagalil and Nabiela M. ElBagir

  • Article Number: 6F15B6311264

November 2009

Immunopurification of a rape (Brassica napus L.) seedling lipase

  Lipase or triacylglycerol acylhydrolase (E.C.3.1.1.3) was purified to homogeneity from rapeseed-germinated cotyledons (Brassica napus L.). The purification scheme involved homogenization, centrifugation, ultracentrifugation and affinity chromatography using polyclonal antibodies raised against porcine pancreatic lipase. The purified rapeseed lipase was homogenous and did not contain...

Author(s): H. Belguith, S. Fattouch, T. Jridi and J. Ben Hamida

  • Article Number: A50B89B11285

November 2009

Effect of Gongronema latifolium crude leaf extract on some cardiac enzymes of alloxan-induced diabetic rats

  The effect of Gongronema latifolium on serum cardiac enzymes in alloxan induced diabetic rat models and normal control rats were determined using graded doses of 80% ethanol extract of G. latifolium leaf. The serum activities of aspartate aminotransferase (AST), creatine kinase (CK), creatine kinase isoenzyme (CKMB) and lactate dehydrogenase (LD) were determined in diabetic Wistar albino...

Author(s): E. E. Edet, M. I. Akpanabiatu A. E. Eno, I. B. Umoh and E. H. Itam

  • Article Number: F19D5FD11291

November 2009

Electrolyte profile and prevalent causes of sickle cell crisis in Enugu, Nigeria

  One hundred sickle cell patients aged between 6 - 25 years in steady state who attended Sickle Cell Clinic at University of Nigeria Teaching Hospital, Enugu, Nigeria were selected for this study. Out of this number, only thirty who were eventually admitted in crisis state within one year of this study were selected for subsequent investigations. They included 20 females and 10 males. We also selected thirty...

Author(s): E. O. Ibe, A. C. J. Ezeoke, I. Emeodi, E. I. Akubugwo, E. Elekwa, M. C. Ugonabo and W. C. Ugbajah

  • Article Number: B0B28D511296

November 2009

The role of ascorbic acid in the treatment of Plasmodium Berghei infected mice

  This work aimed at examining the effect of malaria parasites and ascorbic treatments in mice. The relevance of this research derives from the desire to understand the role of ascorbic acid in malaria infection. In this study design, three groups of ten mice each categorized as non-parasitized-non-treated (control), parasitized-non-treated (PnT) and parasitized ascorbic acid treated (P+asT) were used....

Author(s): H. O. T. Iyawe and A. O. Onigbinde

  • Article Number: CBA24E911303

October 2009

Prevention of cadmium-induced alteration in rat testes and prostate lipid patterns by α-tocopherol

Cadmium induced testicular damage has been investigated in α-tocopherol (Vitamin E) pretreated and non-pretreated male rats exposed to a single sub-lethal dose of cadmium in form of CdCl2. Graded doses of vitamin E (75, 150, and 750 mg kg-1 body wt.) were administered daily to rats in separate groups by gavage for 4 weeks while 3 mg Cd kg-1 body wt was administered subcutaneously, 24 hr to the termination of the...

Author(s): M. A. Adaikpoh and F. O. Obi

  • Article Number: 1E8D95C11224

October 2009

The hormone-emotion-behavioural gene-neuromessenger labyrinth: Pertinent questions

  The discovery of behavioural genes has raised the prospect that behaviour may be governed, in part, by the actions of mutant gene products on the brain. These are mostly enzymes or proteins involved in processes related to neurotransmission. In addition, numerous studies have demonstrated the effects of specific hormones on behaviour. Furthermore, the ability of intense emotions to stimulate or suppress the...

Author(s): N. A. Adamafio

  • Article Number: 8F99C0011232

October 2009

Determination of toxic metal concentrations in flame-treated meat products, ponmo

  Cowhides ‘ponmo’ prepared by various and unknown processing methods were purchased from several markets in Lagos and environs and the contents of lead, cadmium, chromium, zinc, mercury and arsenic were determined before and after boiling. Significantly high levels of these metals except cadmium and zinc were found in all the samples obtained after boiling. The highest levels of Pb (1.54 mg.kg-1),...

Author(s): W. Okiei, M. Ogunlesi, F. Alabi, B. Osiughwu and A. Sojinrin

  • Article Number: A7E23FA11243

October 2009

Studies on separation techniques of pomegranate seeds and their effect on quality of Anardana

  The dehydrated seeds of wild pomegranate fruits (Anardana) are used as acidulent in culinary preparations and in making various medicines. For easy separation of seeds, fruits were subjected to sand roasting, hot water dipping and soaking in cold water. Hot water dip for 2 min saved time required for separation of seeds from fruits (80% excluding treatment time and 74% including treatment time). Due to the...

Author(s): Amit Parashar, S. K. Gupta and Ashok Kumar

  • Article Number: 8112B9B11248

October 2009

Antidermatophytic activities of Ixora brachiata Roxb

  The in vitro antifungal activity of Ixora brachiata Roxb. leaf and root extracts were evaluated against three different genera of dermatophytes viz. Microsporum,Trichophyton and Epidermophyton by Dilution Agar Method. The crude extracts ofIxora brachiata root and leaf showed in-vitro antifungal properties and completely prevented the growth of tested...

Author(s): B. Sadeghi-Nejad and S. S Deokule

  • Article Number: D21A82311257

September 2009

Recent advances in the characterization of peptidyl transferase center: Zero-distance labeling of proteins at or near the catalytic site of human 80S or Escherichia coli 70S ribosomes by means of periodate-oxidized tRNA

  Periodate-oxidized tRNA (tRNAox), the 2’,3’-dialdehyde derivative of tRNA, was used as a zero-length active site-directed affinity labeling reagent, to covalently label proteins at the peptidyl transferase center (PTC), the catalytic site of the large ribosomal subunit. When human 80S or E. coli 70S ribosomes were reacted separately with tRNAox positioned at the P-site, in the...

Author(s): Soria Baouz, Anne Woisard, Lila Chenoune, Gustave Aguié, Gérard Keith, Jean-Marie Schmitter, Jean-Pierre Le Caer and Codjo Hountondji

  • Article Number: 8285D1C11197

September 2009

Haematology, plasma enzymes and organ indices of Clarias gariepinus after intramuscular injection with aqueous leaves extracts of Lepidagathis alopecuroides

  Tank-raised Clarias gariepinus (mean total length, 30.05±4.05cm,SD; mean weights, 250.04±50.22gSD) were injected intra-muscularly with two millilitres per kilogram of each of five concentrations (2.00, 2.00, 6.00, 8.00 and 10.00ppm) of aqueous extracts of leaves of Lepidagathis alopecuroides above the lateral line. The fish in the control group were injected with same dose of...

Author(s): U. U. Gabriel, F. G. Obomanu and O. S. Edori

  • Article Number: 0A69FC111207

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