Abbreviation: Afr. J. Biotechnol.
Start Year: 2002
Page 461 of 593, showing 20 records out of 11854 total, starting on record 9201, ending on 9220
DOI: 10.5897/AJB2009.000-9297[Article Number: 538DD9F8214]
Aspergillus sp. MT-0204, a novel strain for producing β-glucosidase, was isolated from soil in this study. The β-glucosidase can hydrolyze glycosides and increase free isoflavones in soy products isoflavones. By examining physiological, biochemical characteristics and comparing its ITS rDNA gene sequence, it was identified as Aspergillus niger, and named A.... Read more.
DOI: 10.5897/AJB09.028[Article Number: CDDF6988224]
This study was designed to investigate elements’ content and anti-microbial effects of two Malaysian plants, Pereskia bleo and Goniothalamus umbrosus. Elements’ analysis was carried out using Energy Dispersive X-ray Microanalysis combined with Variable Pressure Scanning Electron Microscope (EDX, VPSEM). Anti-microbial screening was performed using disc diffusion... Read more.
DOI: 10.5897/AJB09.327[Article Number: 84461BA8234]
The basket conical traps are commonly used to catch Synodontis species by the fishermen in Asa Lake, Ilorin, Nigeria because these species are caught alive to attract higher market values. Fleets of these traps randomly set at the bottom shoreline by fishermen at three different locations around the lake from the dam site to the river source were examined for Synodontis catch in 12 months.... Read more.
DOI: 10.5897/AJB2009.000-9298[Article Number: 4D6E2078248]
A study was carried out to determine the level of microbial contamination and establish the critical control points associated with the processing of a locally produced ‘Robo’ snack from melon seeds in the Abeokuta Metropolis. Samples were collected at different points of processing from randomly selected local producers and subjected to microbiological hazard analysis. The results were used to... Read more.
DOI: 10.5897/AJB2009.000-9299[Article Number: DB73EBC8262]
Ethnopharmacological information indicates that the leaves of Brillantasia nitens are used in the treatment of anaemia in the south eastern states of Nigeria. In this study, the methanol extract of the leaves of B. nitens was tested for haematinic activity in rats using phenylhydrazine (PHZ- 10 mg/kg. po)-induced anaemia. The red blood cell count (RBC), haemoglobin concentration (Hb),... Read more.
DOI: 10.5897/AJB2009.000-9300[Article Number: 25E63C58273]
Heparin was isolated from two bivalve mollusks, Tridacna maxima (giant clam) andPerna viridis (green mussel). The isolated heparin was quantified in crude as well as purified samples and they were estimated as 2.72 and 2.2 g/kg (in crude) and 260 and 248 mg/g (in purified samples) in T. maxima and P. viridis, respectively. The antiproliferative activity of both the samples... Read more.
DOI: 10.5897/AJB09.288[Article Number: 4C563F67852]
Many environmental issues affect peanut production in continuous monocropping system. Deterioration of soil microbial communities, especially for decrease in fungal diversity and increase in fungal pathogens, is widely hypothesized as a keyfactor for decreasing peanut production. In this study, a pot experiment was conducted to investigate the changes in soil microbial... Read more.
DOI: 10.5897/AJB2009.000-9247[Article Number: 4FBDCA56155]
Most agricultural soils contain large reserves of phosphorous, a considerable part of which accumulates as a consequence of regular applications of phosphate fertilizers. Nearly 95 - 99% is present in the insoluble form and hence cannot be utilized by plants. Fungi have been reported to possess greater ability to solubilize insoluble phosphates than bacteria. In the present study, fungal strains isolated from... Read more.
DOI: 10.5897/AJB2009.000-9249[Article Number: 53B36835656]
The successful use of gene technology for complex crop traits and responses to stress environments remains a challenging approach despite its potential. Stable crop yield in drought prone environments has been one of the most studied complex traits in recent years and transgenic crops with better performance have been repeatedly reported. We reviewed the experimental approach of contrasting case studies that... Read more.
DOI: 10.5897/AJB2009.000-9250[Article Number: 9B8E2645665]
Rice diseases are among the most significant limiting factors threatening food productivity. Genetic engineering provides promising strategies to develop and improve disease resistance in rice. This mini-review summarizes the recent progress on studies of genetic engineering for improvement of rice disease resistance against fungal, bacterial and viral pathogens. Improvement of virus resistance can be... Read more.
DOI: 10.5897/AJB2009.000-9251[Article Number: 0FC48815675]
The use of fermented liquid feeds in monogastric animal nutrition is regarded as one of the biosafe methods of animal production. This paper examines bacterial fermentation of feed substrates for production of fermented liquid feeds for pigs and moist feeds for poultry. Emphasis is placed on the interplay of factors affecting feed fermentation and their relationship to feed quality. The resistance of fermented feeds to... Read more.
DOI: 10.5897/AJB2009.000-9252[Article Number: B860BC55682]
Detection of food borne pathogen is of outmost importance in the food industries and related agencies. For the last few decades conventional methods were used to detect food borne pathogens based on phenotypic characters. At the advent of complementary base pairing and amplification of DNA, the diagnosis of food pathogen became rapid and target specific. RNA based methods such as NASBA and Real Time RT-PCR... Read more.
DOI: 10.5897/AJB2009.000-9253[Article Number: 141CF195688]
The use of bromacil based herbicides in agriculture and environmental management is a growing practice with economic importance. Bromacil possesses broad toxicity to many plant species, although, different formulations exist that are used for different purposes in farming systems. There is increasing concern about its use for the control of invasive woody species on South African rangelands; especially its... Read more.
DOI: 10.5897/AJB2009.000-9254[Article Number: 0A2B9E15703]
In both plants and animals, unsaturated fatty acids are considered to be essential membrane components. Also they play key roles in many cellular events. The synthesis and metabolism of unsaturated fatty acid are very complex processes, involving a variety of enzymes and regulated pathways. Most recently, research has focused on identifying genes modulating unsaturated fatty acids and studying the metabolism and... Read more.
DOI: 10.5897/AJB2009.000-9255[Article Number: CEE34805708]
Clone IDS28 of the yeast Pichia pastoris expressing the human iduronate 2-sulfate sulfatase-Like (hIDS-Like) was employed for low-scale production of the recombinant enzyme in a saline culture media without phosphate. The biological activity found was between 7.3 and 29.5 nmol h-1 mg-1 of total protein. It is about 1.73 to 7 times higher than the result obtained with the same... Read more.
DOI: 10.5897/AJB2009.000-9256[Article Number: 8B66A305715]
Stachytarpheta angustifolia is an important and a highly valued medicinal plant ethnobotanically used in the treatment of various diseases. The present study was carried out to evaluate acute and subchronic toxicity in animals and also evaluate the phytochemical profiles of hydroethanolic extract of S. angustifolia (Mill) Vahlplant. S. angustifolia attracted the attention of the researchers... Read more.
DOI: 10.5897/AJB2009.000-9257[Article Number: 2DD51D65728]
Parinari curatellifolia seeds are ethnobotanically used in the treatment of diabetes and other diseases. The seed drug preparations are administered over a long period of time in the treatment of certain disease conditions. The aim of this study was to evaluate the safety of the seed extract through acute toxicity study. Also subchronic toxicity in the animals was carried out by assessing the effects on biochemical... Read more.
DOI: 10.5897/AJB2009.000-9258[Article Number: D396BED6083]
Spondias mombin L. used in traditional medicine because of its antimicrobial properties was found to contain cardiac glycosides, flavonoids, tannins, and anthraquinones in the stem bark. Bioassay-directed fractionation of the methanol extract of S. mombin was carried out with VLC and HPLC. Isolates were evaluated for antimtb activity which led to the isolation of a series of potential molecules. A semi... Read more.
DOI: 10.5897/AJB2009.000-9259[Article Number: 9E988456090]
The genetic variability among individuals of Artemisia capillaris from state of Terengganu, Malaysia was examined by using the random amplified polymorphic DNA (RAPD) technique. The samples were collected from differences regional in Terengganu State. The genomic DNA was extracted from the samples leaves. Fifthty-seven oligonucleotide primers were screened and ten primers were selected (OPA 04, OPA... Read more.
DOI: 10.5897/AJB2009.000-9260[Article Number: 5C3C17F6095]
Typhoid fever is responsible for the deaths of many people annually. However, conventional and time-consuming detection methods for Salmonella Typhi still dominate. By using a molecular based approach, it was possible to identifySalmonella Typhi by amplifying two specific genes (viaB and tyv) and by using RFLP analysis on the 16S rRNA gene as a first step for identification. We were also able to... Read more.
Page 461 of 593, showing 20 records out of 11854 total, starting on record 9201, ending on 9220