Abbreviation: Afr. J. Biotechnol.
Start Year: 2002
Page 461 of 553, showing 20 records out of 11044 total, starting on record 9201, ending on 9220
DOI: 10.5897/AJB07.889[Article Number: 16221EE7436]
Diesel oil, left standing in a laboratory for six months, was used as source for the isolation of Bacillus subtilis, Bacillus cereus, Trichoderma harzanium andTrichothercium roseum. These organisms were found to be hydrocarbon degraders. On further testing, it was found that B. subtilis had higher potential to utilize diesel oil as carbon source. Soil samples were polluted with diesel... Read more.
DOI: 10.5897/AJB2008.000-5041[Article Number: CBDBC507447]
The Rhizobium sp. DehL was produced by heterologous expression of the cloned gene in Escherichia coli. DehL enzyme was purified to homogeneity and characterized. The molecular weights were estimated to be 61 and 31 kDa by gel filtration and SDS-polyacrylamide gel electrophoresis (SDS-PAGE), respectively, suggesting that the enzyme is a dimer. The purified enzyme was specific to the... Read more.
DOI: 10.5897/AJB2008.000-5042[Article Number: 22E38C27461]
Putative endophytic nitrogen-fixing bacteria that actively express nitrogenase gene in rice were determined using reverse transcription-PCR (RT-PCR) technique. The amplified portion of dinitrogenase reductase genes (nifH) from mRNA of rice roots and stems were cloned and sequenced. Phylogenetic analysis of deduced amino acid sequences of isolated nifH clones (117 sequences) revealed that majority of... Read more.
DOI: 10.5897/AJB08.120[Article Number: 52145857471]
The in vitro antibacterial activities of seaweeds belong to Chlorophyceae (Caulerpa racemosa and Ulva lactuca), Rhodophyceae (Gracillaria folifera andHypneme muciformis) and Phaeophyceae (Sargassum myricocystum,Sargassum tenneerimum and Padina tetrastomatica) were studied against both Gram-negative and Gram-positive pathogenic bacteria. Methanolic extracts of all seaweed... Read more.
DOI: 10.5897/AJB2008.000-5043[Article Number: BCACA1D7478]
Aiba Reservoir was sampled between March, 2004 and February, 2005 for physico-chemical studies. Air temperatures ranged from 20.00 – 30.50oC (mean ± s.e: 26.33 ± 0.26oC), water temperature ranged from 25.00 – 35.00oC (mean ± s.e: 29.26 ± 0.19oC), pH ranged from 5.53 - 9.48 (mean ± s.e: 7.98 ± 0.11), dissolved oxygen ranged from 1.75 – 11.20 mgO2/l... Read more.
DOI: 10.5897/AJB2008.000-5044[Article Number: E162E787484]
Accumulation of heavy metals (Cd, Pb, Hg, Cr) in water and plankton of Sarıyar Dam Lake (SDR) was seasonally studied from April, 2000 to December, 2004. In addition, seasonal changes in phytoplankton and zooplankton populations and species abundance were also determinate. Some physio-chemical parameters of water and their correlation with heavy metals were also determinated. The dominant phytoplanktonic algae... Read more.
DOI: 10.5897/AJB2008.000-5045[Article Number: A9718D77489]
Consortia of litter degrading fungal species were developed from different baiting substrates collected in and around Western ghat forest ecosystem, Coimbatore, Tamilnadu, India. Fifty-three litter degrading fungal species were isolated by nylon litterbag technique. The production of endo-b-1,4-xylanase (1,4-b-D-xylan xylanohydrolase, E.C. 22.214.171.124), b-D-xylosidase (1,4-b-xylan xylanohydrolase, E.C.... Read more.
DOI: 10.5897/AJB08.042[Article Number: 30362ED7492]
Nineteen strains of wood-inhabiting pores fungi, representing thirteen species and ten genera, collected from both natural and “anthropic” (urban) ecosystems in Cuba were tested for their ability to decolourise the industrial anthraquinonic dye Acid Blue 62 (AB 62) in laboratory conditions, in both solid and liquid media. On the basis of their decolourisation rate and growth inhibition, seven... Read more.
DOI: 10.5897/AJB2008.000-5046[Article Number: 541BCA97497]
Aspergillus repens isolated from cultivated soils released pectinmethylesterase (PME) into the liquid culture medium during growth. The enzyme preparation was partially purified by ammonium sulphate precipitation and dialysed. The ammonium sulphate-dialysate fraction of the enzyme was separated by molecular exclusion and ion exchange chromatography. The molecular weight of the enzyme was found to be... Read more.
DOI: 10.5897/AJB2008.000-5047[Article Number: C298BC67500]
Partially purified amylase was extracted from the culture medium of Rhizopus sp. grown in potato dextrose broth for 48 h at room temperature by precipitation with 96.9% ethanol. The enzyme was used to hydrolyze sorghum starch. The hydrolyzed product was afterwards formulated into rat feed, which was fed to albino rats for a period of thirty days. The average daily body weight of the albino rats fed... Read more.
DOI: 10.5897/AJB2008.000-5048[Article Number: 69900937501]
The group that includes the lactic acid bacteria is one of the most diverse groups of bacteria known and these organisms have been characterized extensively by using different techniques. In this study, thirty lactic acid bacterial strains were isolated from soils chicken faeces and feathers. A total of nineteen isolates were obtained and by sequential screening for catalase activity and Gram-staining, eight... Read more.
DOI: 10.5897/AJB2008.000-5049[Article Number: 149B5887503]
Woodfordia fructicosa and Betula utilis were extracted with solvents of different polarities. Antioxidant activities and anti-inflammatory activity of the extracts were evaluated by a 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH), 2,2’-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and lipoxygenase inhibition assay. Ascorbic acid, gallic acid and indomethacin were used as... Read more.
DOI: 10.5897/AJB2008.000-5050[Article Number: C89E5407504]
The effects of some factors on protoplast isolation and regeneration from taxol-producing fungus Ozonium sp. BT2 were investigated, including the enzymolysis time and temperature, the osmotic pressure stabilizer, mycelial incubation time, the culture medium, the culture methods and preprocessing. The mycelia were digested by enzyme combination of 1.5% lywallzyme, 0.5% snailase, 1.5% cellulase... Read more.
DOI: 10.5897/AJB2008.000-5051[Article Number: 5BED1AE7507]
A micro-propagation system was developed for mangosteen (Garcinia Mangostana L.) using leaf sections and mature seeds. This study investigated the effect of various modified Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurin (BAP) alone or in combination with α-naphthalene acetic acid (NAA) on multiple shoot induction from leaf and half seed explants. Among the treatments,... Read more.
DOI: 10.5897/AJB2008.000-5052[Article Number: 42F79E67508]
Dwarf chickling (Lathyrus cicera L) is an important forage legume crop. However, due to the presence of neurotoxin amino acid (b-N-oxalyl-L-a,b-diaminopropionic acid) in the dwarf chickling, its cultivation is limited. There is need to carry out studies for the development of varieties with reduced or none of this neurotoxin for beneficial and prolong consumption by animals. The study reports tissue... Read more.
DOI: 10.5897/AJB08.038[Article Number: 95F99537510]
The batch removal of copper (II) ions from aqueous solution and wastewater using the activated carbon prepared from Gracilaria by acid decomposition was investigated. The effect of pH, biosorption time, adsorbent dose, and metal ions concentration, were considered. The most effective pH was found to be 4.0. The biosorption capacities were solution pH dependent and the maximum uptake for copper with... Read more.
DOI: 10.5897/AJB2008.000-5053[Article Number: FBFEB9C7512]
Twenty seven combinations of process variables were developed and used to produce crude extracts of Mycoplana dimorpha. Crude extracts containing 2,4-dichlorophenol degrading enzymes, were immobilized on sodium alginate beads and degradation studies was conducted in a packed bed column. The rate of degradation of 2,4-dichlorophenol by immobilized crude extracts of was measured at different time intervals... Read more.
DOI: 10.5897/AJB07.486[Article Number: A12EC627514]
Nearly one third of protein in the body consists of collagen. It plays a key role in providing the structural scaffolding for cells, tissues, and organs. With available literature, we show that prolonged exposure of cultures of human connective-tissue cells to ascorbate (vitamin C) induces an eight-fold increase in the synthesis of collagen with no increase in the rate of synthesis of other proteins. Making... Read more.
DOI: 10.5897/AJB08.134[Article Number: 4D649837516]
Lactic acid bacteria (LAB): Streptococcus thermophilus TA 040 andLactobacillus delbrueckii spp. bulgaricus Lb 340, were cultured in reconstituted (10%, w/v) skimmed milk with 5 or 10% (w/v) polyfloral or unifloral honey. Inoculated samples were incubated aerobically at 42°C until milk coagulation. Samples were collected at 2 h intervals and examined for biomass and pH changes.... Read more.
DOI: 10.5897/AJB2008.000-5054[Article Number: C702FF47518]
The purpose of the present investigation is to enhance production of the biomedically important enzyme, lipase, by subjecting the indigenous lipase producing fungal strain Aspergillus japonicus MTCC 1975 to strain improvement and random mutagenesis (UV irradiation, HNO2 and N-methyl-N’-nitro-N-nitroso guanidine). The isolation of mutants and the lipolytic activity of selected mutants were... Read more.
Page 461 of 553, showing 20 records out of 11044 total, starting on record 9201, ending on 9220