Abbreviation: Afr. J. Biotechnol.
Start Year: 2002
Page 461 of 599, showing 20 records out of 11977 total, starting on record 9201, ending on 9220
https://doi.org/10.5897/AJB2009.000-9308[Article Number: 2FCD9267850]
Cowpea aphid-borne mosaic virus (CABMV) is a potyvirus that infects cowpea causing significant yield reduction. However, there is no durable natural resistance to the virus within the crop and genetic engineering for virus resistance was not possible because of a lack of an efficient, reliable and reproducible cowpea transformation and regeneration protocol. Coat protein-mediated resistance to CABMV was... Read more.
https://doi.org/10.5897/AJB09.034[Article Number: 70025C57863]
Rhubarb is an important traditional Chinese herbal drug with high secondary metabolites that interfere with DNA extraction procedures and downstream applications, such as DNA restriction and amplification. An effective and low-cost protocol for isolating genomic DNA from root of Rheum tanguticum is described in this paper. It involved a modified CTAB method with distilled water pretreating samples.... Read more.
https://doi.org/10.5897/AJB09.333[Article Number: 461F4CF7874]
Trichophyton verrucosum is a zoophilic dermatophyte, which causes dermatophytosis infection in human as well as animals. 26S proteasome is an important protein in eukaryotic cells that is involved with degradation of unneeded or damaged proteins, when tagged with ubiquitin. In this study, we characterized the 26S Proteasome regulatory subunit gene in dermatophyte pathogen T. verrucosum.... Read more.
https://doi.org/10.5897/AJB09.462[Article Number: 0BCAAA97892]
Caligula japonica (Lepidoptera: Saturniidae), an endemic species in Eastern Asia, is not only an economically important pest to be controlled, but also a producer of expensive silk. We have demonstrated previously the presence of a higher genetic diversity and a certain degree of genetic differentiation related to geographical distribution in C. japonica based on RAPD and ISSR markers. In this... Read more.
https://doi.org/10.5897/AJB09.583[Article Number: D83D46E7906]
This study aimed to investigate polymorphisms of Calsarcin-1 gene and evaluate its effect on carcass traits in 429 samples of 3 Chinese indigenous cattle breeds, namely, Luxi (LX), Nanyang (NY) and Jiaxianred (JXR) breeds, PCR products with a 320 bp fragment of the Calsarcin-1 gene spanning over a part of intron 2, complete exon 3 and a part of intron 4 were amplified and sequenced. A synonymous... Read more.
https://doi.org/10.5897/AJB08.1062[Article Number: E0DCE237931]
Although in the Mediterranean region the most important Orobanche weed attacking leguminous crop is Orobanche crenata, recently Orobanche foetida has been found attacking faba bean, vetch and chickpea in Tunisia and vetch in Morocco. In this study we have identified and map the quantitative trait loci (QTL) controlling resistance to O. foetida in faba bean (Vicia faba) and studied... Read more.
https://doi.org/10.5897/AJB09.184[Article Number: C7AB6B97969]
The genetic diversity of 219 accessions of Guinea yam germplasm from Benin, Congo, Côte d’ Ivoire, Equatorial Guinea, Gabon, Ghana, Nigeria, Sierra Leone and Togo was accessed using 15 microsatellite loci. High diversity of 0.677 was found among the accessions. An allelic average of 8.06 and polymorphic information content (PIC) value of 0.65 was observed for the markers. The observed... Read more.
https://doi.org/10.5897/AJB09.203[Article Number: 00AA34E8081]
The Cananga odorata callus was initiated from petals of the C. odorata flowers on MS medium and B5 vitamins containing 30 g/L sugar and 3 g/L agar. The medium was also supplemented with different concentrations of 1-naphtalene acetic acid (NAA), and combinations of NAA with 6-benzylaminpurine (BAP) as the plant growth regulators. It was observed that the C. odorata callus could... Read more.
https://doi.org/10.5897/AJB09.421[Article Number: E3DF0A78097]
In this research, several physico-chemical properties and mineral contents of one earliest (May 19) sweet cherry type grown in Konya region were determined. The results in terms of chemical properties were total soluble solids, 18.33 mg/100 g;pH, 3.86; color, S41P50E41; texture, 0.25 kg/cm2; and juice yield, 66.28%. The evaluation of the total weighted rankit... Read more.
https://doi.org/10.5897/AJB2009.000-9309[Article Number: A5731CD8291]
The beneficial impact of arbuscular mycorrhizal (AM) fungi on banana nutrition and resistance against abiotic and biotic stresses is well documented. However, most studies were conducted under greenhouse or field conditions and none reported the life cycle of the AM fungi on banana roots. It is obvious that any system associating both organisms under strict controlled in vitro culture conditions may... Read more.
https://doi.org/10.5897/AJB2009.000-9310[Article Number: D7676128301]
The physicochemical properties and distribution of Pb, Cd, Cr and Ni in soils and growing plants around refuse dumpsites in Akure, Southwestern Nigeria were evaluated to ascertain the impact of the wastes on these soil quality indices and edible plants. The soils close to the dumpsites were more acidic (pH, 5.50) compared with the soils away (25.0 m) from the sites. Also there were corresponding increases in... Read more.
https://doi.org/10.5897/AJB09.050[Article Number: 0E7CAA58376]
A semi-continuous leachate treatment process was developed in the present study. The fresh leachate was obtained from a municipal solid waste transfer station and palm oil mill effluent (POME) sludge was used as sources of anaerobic microbial complex. The semi-continuous treatment of leachate was operated in two phases; in Phase 1, the pH of the bioreactor was not adjusted, and in Phase 2, the pH of the... Read more.
https://doi.org/10.5897/AJB09.238[Article Number: 56476478434]
This work is aimed to assess the aerobic biodegradation of biodiesel/diesel blends (0, 2, 5, 20 and 100%, v/v) by Candida viswanathii. The biodegradation potential of the inoculum was assessed with the redox indicator 2,6-dichlorophenol indophenol (DCPIP) test and with respirometric experiment in biometer flasks (250 mL) used to measure the microbial CO2 production. In the latter, the inoculum was... Read more.
https://doi.org/10.5897/AJB09.326[Article Number: D78A3BC8470]
Drought and water shortage have become a world-wide problem in recent years with the effect of global warming. Water-saving agriculture has become an inevitable direction of agronomical research, it has been developed from practices through agronomic water saving to engineering water saving and to the approaches in biological water-saving. The essence of biological water-saving should be the high-efficient use of water... Read more.
https://doi.org/10.5897/AJB09.242[Article Number: 2EF86419395]
In this work, the characterization of a chitosanase-producing bacterium isolated from soil was reported and this strain was grouped under the genus Aeromonas by virtue of its morphological, physiological properties and 16S rDNA gene sequences. It is the first report that the genus Aeromonas could produce chitosanase.Aeromonas sp. HG08 could secrete the chitosanase (named AsChi) with... Read more.
https://doi.org/10.5897/AJB2009.000-9311[Article Number: 52A94E08647]
Atomic force microscopy (AFM) has been used to visualize the morphological change on the surface of Chinese hamster ovary (CHO) cell membranes before and after electromagnetic pulses (EMP) irradiation. The results show that there were different sizes and shapes of membrane perforate (width ranging from 0.39 - 0.66 μm; depth of 392.95 nm) after 200 pulses of EMP irradiation at 400 kV/m. The results suggested that EMP... Read more.
https://doi.org/10.5897/AJB09.173[Article Number: A6A97398677]
Several fish samples from the Lagos lagoon, Nigeria were analyzed for the presence of polychlorinated biphenyls (PCBs). The fish species analyzed includeTilapia zilli (red belly Tilapia), Ethmalosa fimbriata (Bonga shad) and Chrysichthys nigrodigitatus (catfish). Eight PCB congeners were identified and quantified in muscle of the species analyzed. The concentration of total PCBs in... Read more.
https://doi.org/10.5897/AJB2009.000-9312[Article Number: 7398B218684]
The study investigated the effect of cassava effluent on Okada natural water. It was observed that the colour, taste and odour of the water changed after cassava effluent had been discharched into it. This was an indication of pollution. The physico-chemical analysis showed that the characteristics of water analysed varied and higher than federal environmental protection agency of Nigeria (FEPA). The BOD was in the... Read more.
https://doi.org/10.5897/AJB2009.000-9313[Article Number: F05C29A8689]
Total phenolic content of some fruits and vegetables namely; garden egg (Allium sativvum L.), ducanut (Irvingia wombolu), lemon (Citrus limonia), garden egg (Solanum melongena), grape (Citrus paradise) and carrot (Daucus carota L.) purchased from an open market in Benin City, Nigeria were determined. The results obtained showed that ducanut had the highest amount of total phenolics (98.77... Read more.
https://doi.org/10.5897/AJB08.1158[Article Number: 52C73DE8696]
Three varieties of sorghum grains (SK 5912, KSV 400 and KSV 8) undergoing malting process were screened for lactic acid bacteria (LAB) with antimicrobial activity and characterized using standard methods. Out of fifty-seven lactic acid bacteria isolates, eighteen isolates with antimicrobial producing potential were selected for further study. The isolates displayed significant (p < 0.05) inhibitory... Read more.
Page 461 of 599, showing 20 records out of 11977 total, starting on record 9201, ending on 9220