Abbreviation: Afr. J. Biotechnol.
Start Year: 2002
Page 1 of 581, showing 20 records out of 11613 total, starting on record 1, ending on 20
DOI: 10.5897/AJB2016.15604[Article Number: 279B32A61165]
The babassu (Orbignya oleifera Burret) is a palm that contains oilseeds whose biomass can be used for biofuel production. Due to difficulties in germinating the species, in vitro cultivation techniques are sought as a viable alternative to expand studies on germination. The objective of this study was to evaluate different types of tube caps for in vitro growth of babassu seedlings. Thus, zygotic embryos were inoculated... Read more.
DOI: 10.5897/AJB2015.15122[Article Number: 9F7C65461169]
An on-line methanol sensor system was developed using a methanol probe, methanol sensor unit and peristaltic pump. The system was commanded using data acquisition (DAQ) and LabVIEW software. Calibration of the methanol sensor system was done in a medium environment with yeast cells during cells adaptation to methanol metabolism after glycerol feeding was stopped. The correlation equations between voltage output signal... Read more.
DOI: 10.5897/AJB2016.15366[Article Number: 1E1423A61173]
Hybrid promoters are created by shuffling of DNA fragments while keeping intact regulatory regions crucial of promoter activity. Two fragments of alcohol dehydrogenase (Adh) promoter from Zea mays were selected to generate hybrid promoter. Sequence analysis of both alcohol dehydrogenase promoter fragments through bioinformatics tools identified several crucial cis regulatory elements and transcription factors binding... Read more.
DOI: 10.5897/AJB2015.14610[Article Number: 5D9291061182]
Cellulases are hydrolases of great importance to industries, especially due to their ability to produce ethanol via hydrolysis of cellulolytic materials. Actinomycetes are the producers of these enzymes, particularly the genus Streptomyces sp. The present study is the first report on the production and characterization of cellulolytic complex secreted by Streptomyces capoamus, isolated from the rhizosphere soil of... Read more.
DOI: 10.5897/AJB2016.15256[Article Number: 6AC75B261055]
Diabetes is an endocrine chronic disease that causes high blood sugar level, produced by retardation in, or deficiency of, glucose metabolism in the body of the individual with the disease. Neuropathies and/or angiopathies are complications of diabetes that result in changes in the lower limbs which subsequently evolve for the diabetic foot. Diabetic foot represents one of the most devastating complications of diabetes... Read more.
DOI: 10.5897/AJB2016.15600[Article Number: E3EEA1E61057]
In this study, a wild (LPF-5) and a mutant (HN1) strain of A. niger were compared for lipase production. Several physical parameters (carbon source, nitrogen source, pH, temperature and incubation period) were optimized for maximization of lipase production. Lipase activity between wild type and mutant strain were compared. Among all carbon sources, mixture of glucose (1%, w/v) and olive oil (1%, v/v) exhibited maximum... Read more.
DOI: 10.5897/AJB2016.15541[Article Number: 15AD26761059]
Protease has gained a very important position in many industries such as food, pharmaceutical, chemical and leather industries. In this research, protease was obtained from bacteria. The bacterial strain was obtained from soil which was collected from different areas of Lahore, Pakistan. Fermentation medium (by using sub-merged fermentation technique) was incubated for 48 h at 37°C temperature and agitation speed of... Read more.
DOI: 10.5897/AJB2016.15404[Article Number: 9BD534561060]
North Eastern Ethiopia is a major sorghum-growing region. A total of 415 sorghum landraces were sampled to represent the range of agro-ecologies (three altitude ranges) as well as spatial heterogeneity, that is, 4 zones: North Welo, South Welo, Oromiya and North Shewa with each zone containing 2 to 5 districts. The landraces were genotyped with simple sequence repeats (SSR) and inter simple sequence repeats (ISSR)... Read more.
DOI: 10.5897/AJB2016.15612[Article Number: 810B07061063]
Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) has now become one of major rice diseases. During recent years, its occurrence has increased in Pakistan, particularly in ‘Kallar tract’ which is well-known for quality rice cultivation. Development of new resistant varieties by utilizing the available resistant gene-pool is most reliable breeding technique for the plant breeders. The... Read more.
DOI: 10.5897/AJB2016.15520[Article Number: 571CAEE61064]
This study aimed to evaluate the drying kinetics of Baru (Dipteryx alata Vog), fit different mathematical models to the experimental values as a function of the water content, determine the diffusion coefficient and energy of Baru almonds during the drying process. Baru almonds were dried at 50, 60 and 70°C temperatures in a tray dryer until the water content reached 13% b.u. A non-linear regression analysis was... Read more.
DOI: 10.5897/AJB2015.14981[Article Number: F970CBA61067]
The biochemistry and histopathology of intraperitoneally administered potassium permanganate was investigated in Clarias gariepinus. Acute toxicity of the KMnO4 was determined by intraperitoneally injecting the fish with 0.0, 1.5, 2.0, 4.0 and 6.0 mg/kg. The 96 h lethal concentration (LC50) value obtained from the intraperitoneal injection of the juveniles of C. gariepinus with KMnO4 was 2.001 mg/kg KMnO4 at 95%... Read more.
DOI: 10.5897/AJB2016.15275[Article Number: 6030A6F61069]
Bacterial strains, capable of producing ethanol by using cooking oil glycerol waste (derived from biodiesel production) as sole C-source were screened from soil samples. Based on 16S rRNA sequence, the selected strain was identified as Enterobacter aerogenes G2WG. The sequence was submitted to GenBank and the accession number KU315428 was obtained. The genetic nature of the wild type strain was altered for enhancing... Read more.
DOI: 10.5897/AJB2016.15614[Article Number: F1B3D0B61071]
Bioflocculant from marine Bacillus pumilus JX860616 was characterised and its flocculation mechanism determined. The bacterium was identified by 16S rRNA and the bioflocculant was obtained through solvent extraction after optimum medium composition and culture conditions were established. The physicochemical analysis of the bioflocculant were obtained by scanning electron microscopic (SEM) equipped with elemental... Read more.
DOI: 10.5897/AJB2016.15213[Article Number: 689DE0460810]
Enset is an important food crop produced in Ethiopia with great role in food security. The demand of the crop is increasing throughout the country. However, the production as a whole is decreasing due to devastation by enset bacterial wilt. The studies were conducted to develop the procedure for obtaining multiple disease free plantlets from infected enset plants. Shoot tip explants from infected suckers of three clones... Read more.
DOI: 10.5897/AJB2016.15278[Article Number: C3A068A60815]
Many quantitative trait loci (QTLs) in different barley populations were discovered for resistance to Puccinia hordei and heterologous rust species. Partial resistance (host basal resistance) and nonhost resistance (nonhost basal resistance) in barley to leaf rusts are based on prehaustorial mechanism of resistance which is associated with papillae formation. They are mainly governed by genes with relatively small,... Read more.
DOI: 10.5897/AJB2016.15623[Article Number: 3001A7F60834]
The objective of this work was to identify, through the use of multivariate analysis, the spontaneous accesses of castor bean that show promising characteristics for inclusion in genetic improvement programs of this culture in Brazil. The study was conducted at the Paulista State University, Jaboticabal Campus. The accesses of castor bean seeds obtained were identified as ACS-001-CRSP, ACS-001-JASP, ACS-001-MASP,... Read more.
DOI: 10.5897/AJB2016.15563[Article Number: 1346E3D60837]
Genetic diversity and relationship among 48 coconut individuals (Cocos nucifera L.) collections from the Coastal lowland of Kenya were analyzed using 15 simple sequence repeat (SSR) primer pairs. Diversity parameters were calculated using Popgene Software version 1.31. The gene diversity values ranged from 0.0408 (CAC68) to 0.4861 (CAC23) with a mean value of 0.2839. The polymorphic information content (PIC) values... Read more.
DOI: 10.5897/AJB2016.15418[Article Number: A542F8260840]
Due to the intensive use of pesticides within the greenhouse-rose production, remediation of polluted soils has become a hot topic for researchers in recent decades. Several bacterial strains having the ability to utilize various pesticides as a sole source of carbon and energy were isolated from pesticide-contaminated soils of Urcuquí, Ecuador. According to phenotypical, physiological, and biochemical... Read more.
DOI: 10.5897/AJB2016.15484[Article Number: 7EDFC2760844]
The present work aims to optimize the heat drying conditions of a bioactive feed ingredient derived from the African opaque sorghum beer. The bioactive ingredient was dried at various temperatures 35 to 50°C and times 5 to 24 h. The effects of the drying conditions on the dry matter, water activity, pH, titratable acidity, bacteriocin, ethanol, lactic acid, yeasts, lactic acid bacteria contents and antimicrobial... Read more.
DOI: 10.5897/AJB2016.15580[Article Number: 9A26B8160847]
Chloris virgata, an alkali-tolerant halophyte, was chosen as the test material for our research. The seedlings of C. virgata were treated with varying salt and alkali stress. First, the composition and content of organic acids in shoots were analyzed and the results indicated that there was not only a significant increase in total organic acids, but there were also obvious changes in different components of organic... Read more.
Page 1 of 581, showing 20 records out of 11613 total, starting on record 1, ending on 20