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Full Length
Research Paper
Cytotoxicity of extracts from fruit plants against leukemic
cell lines
Chadarat Ampasavate1,
Siriporn Okonogi1 and Songyot Anuchapreeda2*
1Department
of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai
University, Chiang Mai, 50200, Thailand.
2Division
of Clinical Microscopy, Department of Medical Technology,
Faculty of Associated Medical Sciences, Chiang Mai
University, Chiang Mai, 50200, Thailand.
*Corresponding author. E-mail:
songyot@chiangmai.ac.th.
Accepted
3 December, 2009
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Abstract |
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This
study examined the antileukemic activity of non edible parts
of 13 common Thai tropical fruits. Their ethanolic extracts
were tested for cytotoxic effects on U937, K562, HL60, Molt
4 and normal human peripheral blood
mononuclear cells (PBMCs). Three of 20 crude
plant extracts (kaffir lime leaves, mangosteen peels, and
wampee leaves) had strong
cytotoxic effects on K562, U937, and Molt4 cells. The IC50
values of kaffir lime leaves on those cells were 26.1, 9.0
and 11.9 µg/ml respectively, whereas those of mangosteen
peel were 23.6, 4.5 and 10.1 µg/ml, and those of wampee
leaves were 71.9, 13 and 70.4 µg/ml. Furthermore,
pomegranate peel extract had a potent cytotoxic effect on
HL60 cells (IC50 of 8.0 µg/ml), but was non-toxic
to normal PBMCs, indicating that as a potential source of
antileukemic agents.
Key
words:
Fruit
plants, cytotoxicity, leukemic cell line. |
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