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Full Length
Research Paper
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Biomechanical evaluation of fracture healing following
administration of Piper sarmentosum in ovariectomised
rats
Mohamed Abdalla Estai1, Farihah Suhaimi1,
Ahmad Nazrun Shuid2, Srijit Das1,
Shahrum Abdullah3 and Ima-Nirwana Soelaiman2*
1Department
of Anatomy, Faculty of Medicine, Universiti Kebangsaan
Malaysia, Jalan Raja Muda Abdul Aziz,
50300 Kuala
Lumpur, Malaysia.
2Department
of Pharmacology, Faculty of Medicine, Universiti Kebangsaan
Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur,
Malaysia.
3Department
of Material and Mechanical Engineering, Faculty of
Engineering, Universiti Kebangsaan Malaysia,
43600 Selangor,
Malaysia.
*Corresponding
author. E-mail:
imasoel@medic.ukm.my ,
abdalla177@gmail.com . Tel:
03-92897263. Fax: 03-26989506.
Accepted 7
December, 2011 |
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Abstract |
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The
influence of osteoporosis on fracture healing is
a complex phenomenon. Oxidative stress is
considered as a pathogenic factor for bone loss
and delayed fracture healing. Piper
sarmentosum is known to possess an
antioxidant and anti-inflammatory properties.
This study aimed to investigate the fracture
healing properties of P. sarmentosum
aqueous extract in ovariectomised rats by
observing changes in the biomechanical
properties of femora. Forty female
Sprague-Dawley rats (200 to 250 g) assigned into
4 groups: Sham-operated; ovariectomised-control;
ovariectomised + conjugated
equine oestrogen,
100 µg/kg/day, and ovariectomised + P.
sarmentosum extract, 125 mg/kg/day. All the
rats underwent mid-diaphyseal closed fracture of
the right femur with K-wire fixation 6 weeks
post-ovariectomy. Following the fracture, all
the rats received the aforementioned treatment
orally for 6 weeks. Biomechanical analysis
revealed that, the flexure load, flexure stress
and Young’s modulus for the P. sarmentosum-treated
group increased significantly compared to the
control group (P<0.05). However, the flexure
strain was consistent among all the groups
(P>0.05). The biomechanical strength parameters
in the sham, oestrogen and P. sarmentosum
groups were identical (P>0.05). In conclusion,
effective supplementation of P. sarmentosum
extract restored the biomechanical property of
the healed bone. Hence, it may be beneficial for
fracture healing in the oestrogen deficient
state.
Key words:
Antioxidant, biomechanics, fractures healing, osteoporosis,
Piper sarmentosum.
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