Two new phenolic constituents from Clematis connata
Lin Yuan1, Wenzhong Huang1, Yinhai Ma*
and Zhizhi Du2
Science and Technology Department, Kunming University,
Kunming 650214, P. R. China.
Key Laboratory of Phytochemistry and Plant Resources in West
China, Kunming Institute of Botany, Chinese Academy of
Sciences, Kunming 650204, P. R. China.
*Corresponding author. E-mail:
email@example.com. Tel: +86-871-5098482. Fax:
Abbreviations: NMR, Nuclear magnetic resonace; UV,
ultraviolet; IR, infrared; TLC, thin layer chromatography;
1H-1H COSY: 1H-1H
correlation spectroscopy; HSQC, heteronuclear single quantum
correlation; HMBC, heteronulear multiple bond correlation.
Accepted 20 March, 2012
Two new phenolic compounds,
acetate (2), together with fifteen known
compounds, 3 to 17, were isolated from the dried
whole plants of Clematis connata DC.
Their structures were elucidated by extensive
spectral analysis, especially 2D NMR techniques.
Compounds 1 to 5 were evaluated for their in
vitro cytotoxicities against acute
promyelocytic leukemia HL-60, hepatocellular
carcinoma Hep-G2, oropharyngeal epidermoid
carcinoma KB and breast cancer cells MDA-MB-231.
Compounds 1 and 3 exhibited the most potential
with IC50 values of 0.63 and 1.27 μM
against MDA-MB-231 and HepG2, respectively. The
antibacterial activities experiment of all the
isolated compounds showed that only Compounds 3
to 5 exhibited weak activities against
Staphylococcus aureus, methcillin-resistant
S. aureus and
S. aureus, and Compound 9 and 17 showed
weak activities against S. aureus.
DC., phenolic constituents, cytotoxicities, antibacterial