|
Full Length
Research Paper
|
|
Investigation of quercetin stability in cell culture medium:
Role in in vitro experiment
Jun Hu1, Long Chen1, Fan Lei1,
Yu Tian1,2, Dong-Ming Xing1, Yu-Shuang
Chai1, Shuang Zhao1, Yi Ding1,2
and Li-Jun Du1*
1Protein
Science Laboratory of the Ministry of Education, Laboratory
of Pharmaceutical Sciences, School of Life Sciences and
School of Medicine, Tsinghua University, Beijing 100084,
China.
1Drug
Discovery Facility, Tsinghua University, Beijing 100084,
China.
*Corresponding author. E-mail:
lijundu@mail.tsinghua.edu.cn. Tel/Fax: 86-10-62773630.
Accepted 23 March, 2012 |
|
|
|
Abstract |
|
|
The aims of this work are to investigate the
in vitro stability of quercetin, a focused
compound in food components and a candidate
drug, and to supply the essential information
for the experimental researches of quercetin.
The stability of quercetin in Dulbecco’s
modified Eagle’s medium (DMEM) and H2O
at the cell culture pH range of 6 of 8 at
continued time points, as well as 6 frequently
used supplements in DMEM was investigated. The
assay of high-performance liquid chromatography
(HPLC) was used for quercetin determination.
Considering the stable conditions of quercetin,
the transport behavior of quercetin in Neuro-2a
(N2a) cells was investigated at the dosages of
58.8 and 7.83 μg/ml at 37°C for different time
points. It is shown that quercetin remained
stable in DMEM at pH of 6 with or without 10 mM/L
HEPES, 5% D (+)-glucose, 110 mg/L sodium
pyruvate, antibiotics (50 IU/ml penicillin and
0.05 mg/ml streptomycin), or 10% fetal bovine
serum (FBS), but was decreasing at pH of 7 or 8
with or without 3.7 g/L NaHCO3.
Quercetin transported through N2a cells in 10
min in both dosages of 58.8 and 7.83 μg/ml. Our
data demonstrate that the culture conditions of
quercetin in vitro should be considered
to acquire the convictive results in the
experiments.
Key words:
Quercetin, stability, high performance liquid chromatography
(HPLC), in vitro, transportation. |
