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Full Length
Research Paper
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Effects of Angelica polysaccharide on erythrocyte
immunity and marrow hematopoiesis in chickens
Haifeng Hou1,2, Qian Li3, Wanyu Shi1
and Yongzhan Bao1*
1College
of Veterinary Medicine, Agricultural University of Hebei,
Baoding 071001, P. R. China.
2Baoding
Vocational and Technical College, Baoding 071051, P. R.
China.
3Hebei
Institute of Animal Science and Veterinary Medicine, Baoding
071000, P. R. China
*Corresponding author.
E-mail:
baoyongzhan2006@126.com. Tel: 86-312-7528375.
Accepted 14 March, 2012 |
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Abstract |
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To investigate the effects of Angelica
polysaccharide on erythrocyte immunity and
marrow hematopoiesis in chickens, Angelica
polysaccharide was used for the study of
immunity and hematinic mechanism in order to
provide basis for clinical reference. In this
experiment, three gradient dosages (50, 100, 150
mg/kg body weight) of Angelica
polysaccharide were drenched to the control
group and the anemia groups, respectively. The
anemia chickling model was made by abdominal
injection of cyclophosphamide (CY) for 6 days
(80 mg/kg·day).Red blood cell-C3b receptor
(RBC-CR1) and red blood cell-immune complex
(RBC-IC) rosette rates were measured and
analyzed. Ectogenetic semi-solid culture medium
of bone marrow hemopoietic progenitor cells was
used to observe Angelica polysaccharide
and separated serum from Angelica
polysaccharide treatment on the
proliferation of colony-forming unit-erythrocyte
(CFU-E), burst-forming unit-erythrocyte (BFU-E)
and colony-forming unit-granulocyte macrophage (CFU-GM).
The results showed that Angelica
polysaccharide can significantly increase
RBC-CR1 rosette rate in the healthy chicken
groups (p<0.01), but had no more effect on
RBC-IC rosette rate (p>0.05). At the same time,
Angelica polysaccharide can restore the
RBC- CR1 rosette rate and the RBC-IC rosette
rate caused by cyclophosphamide to the normal
level. Serum containing Angelica
polysaccharide can significantly facilitate the
proliferation on CFU-E (p<0.01), BFU-E (p<0.01)
and CFU-GM (p<0.01), but Angelica
polysaccharide had no more direct proliferation
on CFU-E, BFU-E and CFU-GM. This indicated that
Angelica polysaccharide had the
proliferation on hemopoietic progenitor cells of
marrow by the change of hemopoietic factor.
Key words:
Polysaccharide, chicken, erythrocyte, immunity, hematopoiesis. |
