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Full Length
Research Paper
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The traditional Chinese medicine Huang-Lian-Jie-Du-Tang
inhibits hypoxia-induced neuronal apoptosis
Qichun Zhang, Zhilei Qian, Linmei Pan, Huan Li and Huaxu
Zhu*
College of Pharmacy, Nanjing University of Chinese Medicine,
Nanjing, PR China.
*Corresponding author. E-mail:
huaxuzhu@gmail.com.
Tel/Fax: 86-25-86798399.
Accepted 6 December, 2011. |
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Abstract |
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Huang-Lian-Jie-Du-Tang (HLJDT) has been used
clinically for cerebral ischemia therapeutics.
Here, we aimed to demonstrate whether and how
HLJDT regulates neuronal apoptosis under
hypoxia/ischemia. Apoptosis analysis was
performed in vitro in PC12 cells with
flow cytometry and in vivo in MCAO rats
using TUNEL staining. The levels of caspase 9,
caspase 3, Bcl-2, Bax and HIF-1α were
demonstrated with western blotting. HLJDT
remarkably inhibited apoptosis of neurons, both
in vitro and in vivo. Caspase 9,
caspase 3m Bcl-2, Bax and HIF-1α play important
roles in anti-apoptotic effect of HLJDT on
neuronal apoptosis hypoxia and ischemia induced.
Key words:
Huang-Lian-Jie-Du-Tang, apoptosis, HIF-1, ischemia.
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