The traditional Chinese medicine Huang-Lian-Jie-Du-Tang
inhibits hypoxia-induced neuronal apoptosis
Qichun Zhang, Zhilei Qian, Linmei Pan, Huan Li and Huaxu
College of Pharmacy, Nanjing University of Chinese Medicine,
Nanjing, PR China.
*Corresponding author. E-mail:
Accepted 6 December, 2011.
Huang-Lian-Jie-Du-Tang (HLJDT) has been used
clinically for cerebral ischemia therapeutics.
Here, we aimed to demonstrate whether and how
HLJDT regulates neuronal apoptosis under
hypoxia/ischemia. Apoptosis analysis was
performed in vitro in PC12 cells with
flow cytometry and in vivo in MCAO rats
using TUNEL staining. The levels of caspase 9,
caspase 3, Bcl-2, Bax and HIF-1α were
demonstrated with western blotting. HLJDT
remarkably inhibited apoptosis of neurons, both
in vitro and in vivo. Caspase 9,
caspase 3m Bcl-2, Bax and HIF-1α play important
roles in anti-apoptotic effect of HLJDT on
neuronal apoptosis hypoxia and ischemia induced.
Huang-Lian-Jie-Du-Tang, apoptosis, HIF-1, ischemia.