Rofiee et al. Involvement of CYP450 system in hepatoprotective activity of Malaysian Agricultural Research and Development Institute (MARDI)-produced virgin coconut oils. Afr. J. Pharm. Pharmacol. 22 December, 2011

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Afr. J. Pharm. Pharmacol.

Vol. 5 No. 23

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Full Length Research Paper

Involvement of CYP450 system in hepatoprotective activity of Malaysian Agricultural Research and Development Institute (MARDI)-produced virgin coconut oils

M. S. Rofiee¹, Z. A. Zakaria²*, M. N. Somchit², A. Zuraini², A. K. Arifah³, L. K. Teh⁴, M. Z. Salleh⁴ and K. Long⁵

¹Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universiti Teknologi MARA, 42300 Puncak Alam, Kuala Selangor, Malaysia.

²Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

³Department of Veterinary Preclinical Sciences, Faculty of Veterinar, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

⁴Pharmacogenomics Centre (PROMISE), Faculty of Pharmacy, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor DE, Malaysia.

⁵Biotechnology Research Centre, Malaysian Agriculture Research and Development Institue, 50744 Kuala Lumpur, Malaysia.

*Corresponding author. E-mail: zaz@medic.upm.edu.my or dr_zaz@yahoo.com.

Accepted 21 April, 2011.

Abstract

The present study aims to determine the role of cytochrome P450 (CYP450) enzyme system in hepatoprotective activity of virgin coconut oils produced by Malaysian Agricultural Research and Development Institute (MARDI). Paracetamol (PCM)-induced hepatotoxic rat was used as a model. Liver injury induced by 3 g/kg PCM increased the liver weight and liver enzymes (e.g. alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphate (ALP)) and decreased cell viability indicating liver damage. Histological observation also confirms liver damage indicated by the presence of inflammations and necrosis. Pre-treatment with VCOA or VCOB reversed the significantly (P < 0.05) reversed PCM toxic effect. Groups pre-treated with virgin coconut oil (VCOs) followed by inhibitor or inducer of CYP450 demonstrated significant (P < 0.0.5) increase in liver weight, liver enzymes levels and decrease in cell viability, which are, however, significantly (P < 0.05) less remarkable as compared to group treated with PCM alone. In conclusion, VCO possessed hepatoprotective effect, which is believed to be mediated via a non-CYP450 system and might be associated partly with the antioxidant potential of the oil. Further studies are warranted to determine the actual mechanisms of hepatoprotection involved.

Key words: Malaysian Agricultural Research and Development Institute (MARDI), virgin coconut oil, liver toxicity, paracetamol, cytochrome P450, hepatoprotection.

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