Full Length Research Paper

Investigation of antibacterial activity of Streptomycetes isolates from soil samples, West of Iran

Alireza Dehnad*, Lalle Parsa, Rouhollah Bakhshi, Samad abdi soofiani and Ahad Mokhtarzadeh

Department of genomics, Branch of Northwest and West region of Iran (Tabriz), Agriculture Biotechnology Research Institute of Iran (ABRII), Iran.

*Corresponding author. E-mail: dehnad@abrii.ac.ir. Tel: 00989143001930.

Fax: 00984113312613

Accepted 22 December, 2009

Abstract

In this research our goal is the determination of Streptomyces species with antimicrobial activity from some regions of northwest of Iran. The future studies will be performed to investigate the type of antimicrobial agents. In order to achieve to this aim, following the collection of the soil samples, the samples were diluted and cultured in SCA medium. The Actinomycetes were isolated considering morphological characteristics in macroscopic and microscopic levels and examined for the microbial activity. The antimicrobial positive bacteria were selected for further biochemical and molecular studies. Through the molecular studies, 16srDNA gene of the each bacterium was amplified and digested using TaqI endonuclease. The handling of RFLP pattern of 16srDNAs using Genedoc bioinformatics software determined the strains of the bacteria. As results of the study out of 150 isolated Actinomycete colonies, 12 different strains showed antimicrobial activity which 11 strain of them belonged to the Streptomycetes and were known as different strains of the Streptomyces genus. We identified 11 Streptomyces strains from mentioned regions of the northwest of Iran with high antimicrobial activity. To determine the antimicrobial agents further studies are needed. In this research we tried to study the ability of the production of anti-microbial agents by Streptomyces species from some regions of Northwest of Iran. Our results led to the 12 isolates with the different ability to produce antibiotics.

Key words: Actinomycetes, antibiotics, antimicrobial activity, Iran, Streptomycetes.