Full Length Research Paper

Isolation and identification of endophytic bacteria antagonistic to Camellia oleifera anthracnose

Li He^1,2, Guoying Zhou^1,2*,  Lili Lu² and Junang Liu^{1, 2}

¹Biotechnology Core Facilities, Central South University of Forestry and Technology, Changsha, 410004, China.

²College of Forestry, Central South University of Forestry and Technology, Changsha, 410004, China.

*Corresponding author. E-mail: csuftlihe@163.com.

Accepted 4 June, 2009

   Abstract

Anthracnose is a common Camellia oleifera disease caused by Colletetrichum gloeosporioide. This study was to isolate antagonistic bacteria from Camellia oleifera tissues to control the anthracnose. Antagonistic bacteria was screened from 175 endophytic bacteria with confront culture method. The results of the study showed that the antagonistic effect of YB128 strain was the best with 86.1% inhibitory rate. According to the results of the researching on morphology, cultural characteristics, physiological and biochemical properties, 16S gene sequence and molecular phylogenetic tree, YB128 was identified as Bacillus licheniformis.

Key words: Camellia oleifera, anthracnose, antagonistic bacteria, biological control